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Quick Search     Adv Search 2014 Vol. 31, No. 3 Published: 2014-03-26   417 Correlation Between FoxO1 Gene rs17446593 Polymorphism and Type 2 Diabetes Mellitus in Li-aoning Province [Objective]To explore the correlation between FOXO1 gene rs17446593 polymorphism and type 2 diabetes mellitus(T2DM ) .[Methods] A total of 395 patients with T2DM and 366 control subjects were chosen .MassARRAY method was used to determine FOXO1 gene rs17446593 polymorphism .SPSS13 .0 sta-tistical software was used for the analysis .[Results] The frequencies of genotype GG ,GA and AA of FOXO1 gene rs17446593 in T2DM group were 1 .72% ,27 .09% and 71 .14% ,respectively ,and those in control group were 1 .64% ,20 .22% and 78 .14% ,respectively (χ2 = 5 .0460 ,P =0 .080 between two groups) .The fre-quencies of G allele in two groups were 15 .32% and 11 .75% ,respectively (χ2 =4 .116 ,P =0 .042 between two groups) .[Conclusion]Allele G of FOXO1 gene rs17446593 may be the predisposing gene of T2DM . 2014 Vol. 31 (3): 417-419 [Abstract] ( 67 ) HTML (1 KB)  PDF  (0 KB)  ( 37 ) 420 Survey on the Prevalence of Hyperhomocysteinemia,Hyperferritinaemia and Hyperuricemia in Middle-age Male Patients with Hyperlipidemia LI Zong-feng%LI Hong-chen [Objective]To investigate and analyze the prevalence of hyperhomocysteinemia ,hyperferriti-naemia and hyperuricemia in middle-aged male patients with hyperlipidemia .[Methods]Male patients(aged 40~60 years old) with hyperlipoidemia and subjects with normal blood lipid and the same age in our hospital were chosen .All patients had no nephrosis and recently had not taken drugs affecting the metabolism of homo-cysteine(Hcy) ,ferritin(Ferr) and uric acid(UA) .The levels of Hcy ,Ferr and UA were determined .[Re-sults] The levels of Hcy(15 .83 ± 5 .79μmol/L) ,Ferr(330 ± 74ng/ml) and UA(380 ± 90μmol/L) in hyperlipi-demia group were higher than those in normal group(8 .58 ± 4 .26μmol/L ,241 ± 87ng/ml and 325 ± 86μmol/L , respectively) ,and there was significant difference( P < 0 .01) .The morbidity of hyperhomocysteinemia(21 . 3% ) ,hyperferritinaemia(21 .6% ) and hyperuricemia(36 .5% ) in hyperlipidemia group were higher than those in normal group(10 .2% ,8 .1% and 16 .8% ,respectively) ,and there was significant difference( P <0 .01) . Different age groups were compared .Hcy level in 50~60 age group was higher than that in 40~49 age group ( P <0 .01) ,but there was no significant difference in the morbidity of hyperhomocysteinemia between these two age groups( P>0 .05) .There was no significant difference in the levels of Ferr and UA and the morbidity of hyperferritinaemia and hyperuricemia between these two age groups ( P > 0 .05) .[Conclusion] Compared with normal blood lipid group ,the morbidity of hyperhomocysteinemia ,hyperferritinaemia and hyperuricemia in middle-aged male patients with hyperlipidemia is higher ,and the levels of Hcy ,Ferr and UA are also high-er .It is important to monitor the levels of Hcy ,Ferr and UA in these special people for clinical practice . 2014 Vol. 31 (3): 420-422 [Abstract] ( 32 ) HTML (1 KB)  PDF  (0 KB)  ( 30 ) 423 Effect of Statins on the Prognosis of Diabetic Patients with Severe Infection [Objective] To explore the effect of statins on the prognosis of diabetic (DM ) patients with se-vere infection .[Methods] Totally 210 patients with DM and severe infection in endocrinology department and intensive care unit(ICU) department of Zhaogezhuang hospital of Kailuan general hospital from July 2011 to July 2013 were selected .All patients were randomly divided into simvastatin group (group T ) and control group(group T ) with 105 patients in each group .Patients in two groups were given the treatment of primary disease ,anti-infection ,nutrition and symptomatic support therapy .On the basis of above treatment ,group T was given oral or nasal simvastatin 40mg from the day of enrollment for 14 days .Acute physiological and chro-nic health score , levels of CRP , TNF-α, IL-6 , IL-10 , the incidence of multiple organ dysfunction score (MODS) and 28d fatality rate in two groups before(T1 ) ,4 d(T2 ) and 7 d(T3 ) after medication were measured and compared .[Results] The levels of IL-6 ,TNF-αand CRP in group T at T2 and T3 were lower than those in group T at T1 and in group C at T2 and T3 ( P<0 .05) ,and the levels of IL-10 were higher than those in group T at T1 and group C at T2 and T3 ( P<0 .05) .The levels of IL-6 ,TNF-αand CRP in group C at T3 were low-er than those at T1 ( P <0 .05) ,but the levels of IL-10 were higher than those at T1 ( P <0 .05) .The inci-dence of MODS in group T at T3 was 8 .57% ,which was obviously lower than that in group C(35 .23% )( P<0 .01) .The 28d fatality rate in group T at T3 was lower than that in group C(3 .81% vs .17 .14% )( P <0 .05) .[Conclusion] Statins for the treatment of DM patients with severe infection can decrease inflammatory response ,reduce the incidence of MODS and the 28d fatality rate and further improve the prognosis of pa-tients .Therefore they have certain clinical application value . 2014 Vol. 31 (3): 423-426 [Abstract] ( 27 ) HTML (1 KB)  PDF  (0 KB)  ( 34 ) 426 Study on the Correlation Between IL-1βRs16944 Gene Polymorphism and Type 2 Diabetes Mel-litus in Han People of Liaoning Province [Objective] To explore the correlation between interleukin-1β(IL-1β) rs16944 gene polymor-phism and type 2 diabetes mellitus(T2DM ) .[Methods] The extraction genomic DNA and MassARRAY mole-cule weight array were used to analyze the gene polymorphism of IL-1βin healthy control group(n=366) and 583 T2DM patients .[Results]The distribution frequencies of 3 genotypes AA ,AG and GG of IL-1βin healthy control group were 22 .40% ,50 .00% and 27 .60% respectively ,while those inT2DM group were 20 .58% , 50 .09% and 29 .33% , respectively . The distribution frequencies of allele A in healthy control group and T2DM group were 47 .40% and 45 .63% ,respectively .The distribution frequencies of allele G in healthy con-trol group and T2DM group were 52 .60% and 54 .37% ,respectively .There was no significant difference in each genotype and allele genotype between two groups ( P>0 .05) .[Conclusion]IL-1βrs16944 gene polymor-phism is probably independent of T2DM in Liaoning province . 2014 Vol. 31 (3): 426-428 [Abstract] ( 26 ) HTML (1 KB)  PDF  (0 KB)  ( 33 ) 429 Study on the Institution and Application of Clinical Pathway in Common Hand-Foot-Mouth Disease [Objective]To evaluate the efficacy of clinical pathway in the treatment of common hand ,foot and mouth disease(HFMD) .[Methods] According to random digit table ,305 patients with HFMD in Xi'an Children's hospital were randomly divided into two groups .The days of hospitalization ,medical cost and other clinical data of 153 patients receiv-ing clinical pathway (experiment group) and concurrent 153 patients without receiving clinical pathway (control group) were compared and analyzed statistically .[Results] The days of hospitalization in experimental group and control group were (4 .56 ± 0 .45)d and (6 .18 ± 0 .63)d respectively ,and the average difference was 1 .6d .The medical cost in experi-mental group and control group were (2158 .57 ± 42 .65)yuan and (4041 .34 ± 35 .23)yuan respectively ,and the average difference was 1882 .77yuan .The days of hospitalization ,the cost of treatment ,examination and medicine and other mis-cellaneous expense in experiment group were obviously lower than those in control group ,and there were significant differences between two groups ( P<0 .01) .The satisfaction degree of experiment group was 98 .69% (151/153) ,which was far higher than that in control group (81 .04% ,124/153) ,and there were significant differences (χ2 =5 .569 ,P <0 .01) .The survey of clinical physicians showed that clinical pathway could reduce the work load .[Conclusion] Clinical pathway can obviously shorten the days of hospitalization ,reduce medical cost ,normalize active medical behavior of physi-cians and nurses ,decrease the repeatable labor and improve the work efficiency . 2014 Vol. 31 (3): 429-431 [Abstract] ( 72 ) HTML (1 KB)  PDF  (0 KB)  ( 30 ) 432 Study on the Correlation of Interleukin-1βGene rs1143627-C/T Polymorphism with Type 2 Dia-betes Mellitus [Objective]To explore the correlation between rs1143627-C/T polymorphism of interleukin-1β(IL-1β) gene and type 2 diabetes mellitus(T2DM) .[Methods]MassArray molecular weight array method was used to de-tect the rs1143627-C/T polymorphism of IL-1βgene in 240 patients with T2DM (DM group) and 245 normal controls (NC group) .Fasting blood-glucose ,blood lipid ,body mass index(BMI) and other clinical indicators were examined .[Results]Genotypic frequencies of IL-1βgene(rs1143627) were 28 .8% (TT) ,52 .5% (TC) and 18 .7% (CC) ,re-spectively in DM group and 23 .3% (TT) ,47 .3% (TC) and 29 .4% (CC) ,respectively in NC group .Allele T fre-quency in DM and NC group was 55 .0% and 46 .9% ,respectively .Allele C frequency in DM and NC group was 45 .0% and 53 .1% ,respectively .There was significant difference in genotypic frequency and allele gene of IL-1β(rs1143627) between two groups( P<0 .05) .[Conclusion]The rs1143627-C/T polymorphism of IL-1βgene may be associated with the incidence and genetic susceptibility of T 2DM . 2014 Vol. 31 (3): 432-436 [Abstract] ( 19 ) HTML (1 KB)  PDF  (0 KB)  ( 33 ) 434 Influence of Spinal-epidural Combined Anesthesia on Perioperative Hemodynamics of Renal Transplantation Patients LIU Xin-jie%GUO Qu-lian [Objective] To explore the perioperative hemodynamic change rule of the recipients undergoing renal transplantation under spinal-epidural combined anesthesia .[Methods]Totally 100 patients undergoing re-nal transplantation were randomly selected .Allograft renal transplantation was performed under spinal-epi-dural combined anesthesia .The changes of hemodynamics were observed .[Results]Systolic blood pressure (SBP) ,mean arterial pressure(MAP) and diastolic blood pressure(DBP) 10min after spinal-epidural combined anesthesia were obviously decreased( P <0 .05) .SBP ,MAP and DBP 20 min after blocking iliac vein were gradually restored to the level before anesthesia .SBP ,DBP and MAP after the opening of blood flow of trans-planted kidney were obviously decreased .[Conclusion]Spinal-epidural combined anesthesia in renal transplan-tation has ideal and exact efficacy .The influence of spinal-epidural combined anesthesia on hemodynamic chan-ges is different at different operation stages .Proper anesthesia management in each stage is the key to keep stable hemodynamic . 2014 Vol. 31 (3): 434-436 [Abstract] ( 21 ) HTML (1 KB)  PDF  (0 KB)  ( 40 ) 437 Effect of CNP-Activated cGMP-PKGIa Signal on Antagonizing 5-HT-induced Vascular Smooth Muscle Cell Contraction LI Yong-jie%ZENG Ding-yin [Objective]To explore the effect of C-type natriuretic peptide (CNP ) on antagonizing 5-hydroxytryptamine(5-HT)-induced aortic smooth muscle cell contraction and its mechanism .[Methods]CNP stimulated A10 aortic smooth muscle cells in vitro by different concentrations (10-5M-10-9M ,respectively ) at different time points(0 ,5 ,10 ,15 ,20 and 30 minutes) .Then enzyme-linked immunosorbent assay(ELISA) was used to detect the changes of cGMP concentration .The expressions of PKGIα and PKGIβ in cells after CNP stimulation were detected by Western blot .The effect of CNP on 5-HT-induced cell contraction was ob-served by confocal microscopy .[Results] The generation of cGMP in smooth muscle cells after CNP treatment was obviously increased in a dose dependent manner ,and markedly increased in the beginning (5min) and reached to the peak around 10min .Meanwhile ,the expression of cGMP downstream molecule ,cGMP-depend-ent protein kinase (PKG ) after CNP treatment was activated which presented the increased expression of PKGIα.After PKG specific inhibitor interfered ,the antagonistic effect of CNP on 5-HT-induced aortic vascu-lar smooth muscle cell contraction was decreased .[Conclusion]CNP can activate cGMP-PKGIa signal against 5-HT-induced aortic vascular smooth muscle cell contraction .It indicates that CNP can be used as an effective target for the prevention and treatment of atherosclerosis . 2014 Vol. 31 (3): 437-441 [Abstract] ( 28 ) HTML (1 KB)  PDF  (0 KB)  ( 31 ) 441 Value of MRI in the Diagnosis of Large Vascular Malformation in Children with Congenital Heart Disease [Objective]To explore the value of magnetic resonance imaging (MRI) in diagnosis of large vas-cular malformation in children with complex congenital heart disease (CCHD) .[Methods]In 22 CCHD children with large vascular malformation confirmed by operation underwent MRI examination of large cardiac vessels . The characteristics of MRI were analyzed .[Results]Of all 22 children with CCHD ,11 children were tetralogy of Fallot ,and 4 children were viscero-atrial heterotaxy syndrome ,and 2 children were vascular rings ,and 6 children were congenital coarctation confirmed by operation .The coincidence rate of results of MRI and pathol-ogy was 100% .Multi-view images and three-dimensional reconstruction image by MRI could clearly show int-racardiac anatomy ,anatomy and morphology of thoracic and abdominal organs and the position of large vessels around the roots .[Conclusion] MRI scanning technology is valuable in the diagnosis of large vascular malfor-mation in pediatric patients with CCHD .Preoperative diagnosis is essential for surgical treatment of congenital heart disease . 2014 Vol. 31 (3): 441-444 [Abstract] ( 26 ) HTML (1 KB)  PDF  (0 KB)  ( 41 ) 445 Expression of PTEN and hMSH2 in Young Women with Endometrial Carcinoma DENG Kai-yu%XU Da-bao%CHEN Yun [Objective] To explore the expressions of tumor suppressor gene PTEN and DNA mismatch repair gene hMSH2 in young women with endometrial carcinoma and their correlation .[Methods]The endometrial specimens of 66 women(≤45 years old) undergoing total hysterectomy for uterine diseases were collected .Among them ,there were 42 cases of endometrial carcinoma ,6 cases of atypical endometrial hyperplasia and 18 cases of normal endometri-um .The expressions of PTEN and hMSH2 in the tissues of endometrium were detected by immunohistochemical SP method .[Results] The expression rates of PTEN in normal endometrium ,atypical endometrial hyperplasia and endo-metrial carcinoma were decreased gradually ,and the absence rates were 16 .67% ,66 .67% and 85 .71% respectively , and there was significant difference ( P < 0 .01) .The expression rates of hMSH2 were 44 .44% ,33 .33% and 73 .81% ,respectively ,and there was significance difference among groups ( P <0 .05) .The expressions of PTEN and hMSH2 had no correlation with clinical stage ,histological grade ,myometrial invasion depth and lymphatic metas-tasis whether or not(rs=0 .271 ,P>0 .05) .There was no significant correlation between hMSH2 and PTEN in en-dometrial carcinoma( P < 0 .05) .The expression of hMSH2 was discordant with the expression of PTEN (χ2 =23 .04 ,P=0 .000) .[Conclusion] The combined detection of PTEN and hMSH2 is helpful for the early diagnosis and can infer the prognosis . 2014 Vol. 31 (3): 445-448 [Abstract] ( 25 ) HTML (1 KB)  PDF  (0 KB)  ( 36 ) 449 Clinical Research on the Risk Factors of Hidden Aspiration in Elderly Cerebral Infarction [Objective]To explore the risk factors and occurrence mechanism of hidden aspiration after elderly cerebral in-farction in order to provide the reference for risk assessment and early intervention of stroke associated pneumonia .[Methods]A total of 72 elderly inpatients with acute cerebral infarction in neurological department of our hospital from Aug .2011 to Oct . 2012 were enrolled .Clinical evaluation of swallowing function was completed within 24h after admission ,and the swallowing TV fluoroscopy(VFSS) was completed within 7d after admission .VFSS was used to confirm patients with or without aspira-tion .The incidence of hidden aspiration was recorded .The correlation of hidden aspiration with age ,lesion site and clinical swallowing abnormal appearance was analyzed .The risk factors and occurrence mechanism of hidden aspiration in senile cere-bral infarction were also analyzed .[Results] Among 72 patients completing VFSS ,32 patients were aspiration in which 13 pa-tients(40 .6% ) were hidden aspiration .The incidence of hidden aspiration was 44 .4% (32/72) .The incidence rates of hidden aspiration in patients aged 60~65 ,66~70 ,71~75 ,76~80 and over 80 years old were 7 .7% ,15 .4% ,23 .1% ,23 .1% and 30 .7% ,respectively .The incidence of hidden aspiration in patients with older age was higher .There was significant difference in incidence of aspiration between the patients aged over 80 years old groups and the patients aged 60~ 65 years old ( P <0 .05) .There was no significant difference among other age groups ( P>0 .05) .The hidden aspiration rate of patients with bi-lateral multiple infarction was 46 .6% (7/15) ,and that of patients with brain stem and small cerebral infarction was 40% ,and that of patients with cerebral hemisphere and basal ganglia infarction was 9 .5% .There was significant difference in hidden as-piration rate between the first two groups and cerebral hemisphere infarction combined with basal ganglia infarction group ( P<0 .01) ,but there was no significant difference between the first two groups ( P>0 .05) .[Conclusion]Senile patients with acute cerebral infarction are prone to have implicit aspiration .The incidence of hidden aspiration in patients with bilateral multiple cerebral infarction and older age is higher . 2014 Vol. 31 (3): 449-451 [Abstract] ( 63 ) HTML (1 KB)  PDF  (0 KB)  ( 35 ) 452 Relationship Between Blood Pressure Variability and Left Ventricular Hypertrophy in Patients with Essential Hypertension WA NG Li%LI Zhi-ping%ZHA NG Hui [Objective] To explore the correlation between blood pressure variability and left ventricular hypertrophy(LVH) in patients with essential hypertension .[Methods] Totally 60 patients with essential hy-pertension were monitored by echocardiogram and dynamic blood pressure .According to left ventricular mass index(LVMI) ,all patients were divided into left ventricular hypertrophy (LVH) group and non-LVH group . Mean blood pressure and blood pressure variability were compared between two groups .[Results] The 24h systolic blood pressure (24hSBP) ,24h diastolic blood pressure (24hDBP) ,daytime systolic blood pressure (dSBP) ,daytime diastolic blood pressure (dDBP) ,night systolic blood pressure (nSBP) and night diastolic blood pressure(nDBP) in LVH group were markedly higher than those in non-LVH group ,and there was sig-nificant difference( P <0 .05) .Compared with non-LVH group ,the indexes of blood pressure variability in LVH group except dDBP were increased ,and there was significant difference( P <0 .05) .[Conclusion] The occurrence of LVH in patients with essential hypertension is closely related to blood pressure variability .The increasing of blood pressure variability is valuable for predicting the risk of LVH . 2014 Vol. 31 (3): 452-454 [Abstract] ( 28 ) HTML (1 KB)  PDF  (0 KB)  ( 37 ) 454 Research on the Correlation of Carotid Atherosclerosis with Pulse Pressure and Pulse Pressure Index in Patients with Hypertension XUE Hai-ping [Objective] To explore the correlation of carotid atherosclerosis with pulse pressure (PP) and pulse pressure in-dex(PPI) in patients with hypertension .[Methods] Totally 162 patients with hypertension were selected .Color Doppler ul-trasound was used to measure carotid intima-media thickness(IMT) in all patients .According to IMT ,all patients were divid-ed into carotid atherosclerosis group(n=114) and carotid normal group(n=48) .The dynamic blood pressure was monitored . PP and PPI were calculated and compared with the results of color Doppler ultrasound .[Results] Systolic blood pressure (SBP) ,mean arterial blood pressure (MBP) ,PP and PPI in carotid atherosclerosis group were markedly higher than those in normalcarotidgroup,andtherewassignificantdifference(P<0.05).IMTinpatientswithPP>60mmHgwas1.37±0.43 cm ,which was markedly higher than that in patients with PP <60mmHg(0 .83 ± 0 .12cm) ,and there was significant differ-ence( P<0 .05) .PP and PPI showed a positive correlation (normal carotid group:r=0 .902 ,P=0 .001;carotid atheroscle-rosis group :r=0 .894 ,P=0 .002) .[Conclusion]Carotid atherosclerosis is closely related with PP and PPI .The combination detection is important to prevent hypertension with carotid atherosclerosis . 2014 Vol. 31 (3): 454-456 [Abstract] ( 33 ) HTML (1 KB)  PDF  (0 KB)  ( 38 ) 457 Study on the Correlation of Serum Lipoprotein (a),pro-BNP,Troponin I and Total Bilirubin with the Prognosis of Patients with Chronic Heart Failure [Objective]To explore the correlation of serum lipoprotein (a) ,pro-BNP ,troponin I and total bilirubin with the prognosis of patients with chronic heart failure .[Methods] A total of 94 patients diagnosed as chronic heart failure in our hospital from Jan .2012 to April 2013 were enrolled as observation group ,and 94 healthy people were included as control group .Serum levels of lipoprotein(a) ,pro-BNP ,troponin I and to-tal bilirubin of two groups were measured and compared .The NYHA classification was applied to evaluate heart function of patients with chronic heart failure ,and the correlation between the above four serum biomar-kers and heart failure was analyzed .[Results]Serum levels of lipoprotein (a) ,pro-BNP ,troponin I and total bilirubin in observation group were higher than those in control group ( P <0 .05) .In observation group ,the above indexes of patients with high grade NYHA were higher than those of patients with low grade NYHA ( P<0 .05) .Logistic regression analysis showed that serum levels of lipoprotein (a) ,pro-BNP ,troponin I and to-tal bilirubin were closely related with the disease ( P <0 .05) .[Conclusion]Compared with control group ,ser-um lipoprotein(a) ,pro-BNP ,troponin I and total bilirubin in patients with chronic heart failure are obviously increased and closely correlated with NYHA class .These biomarkers can reflect the severity of the disease , which suggests that they can be used as the indicators for assessing the prognosis of the disease ,and have clin-ical application value . 2014 Vol. 31 (3): 457-459 [Abstract] ( 60 ) HTML (1 KB)  PDF  (0 KB)  ( 42 ) 460 Analysis of Clinical Efficacy of Laparoscopy Combined with Choledochoscopy for the Treatment of Choledocholithiasis HUANG Rong-miao%ZHU Ming [Objective] To observe clinical efficacy of laparoscopy combined with choledochoscopy for the treatment of choledocholithiasis .[Methods]Totally 120 patients with choledocholithiasis scheduled for opera-tion treatment were randomly divided into two groups with 60 patients in each group .The experiment group underwent the calculi removal by laparoscopy combined with choledochoscopy ,while the control group under-went open operation .The curative effect was compared between two groups .[Results] The operation time , bleeding volume ,postoperative T-tube placement time ,the recovery time of postoperative intestinal function and hospitalization time in experiment group were better than those in control group ,and there were significant differences between two groups( P <0 .05) .The adverse reactions occurred in 6 patients of experiment group (10 .00% ) ,which were significantly lower than those of control group (11 patients ,18 .33% )( P <0 .05) .[Conclusion] Laparoscopy combined with choledochoscopy for the treatment of choledocholithiasis has obvious advantages ,good prognosis and low incidence of adverse reaction . 2014 Vol. 31 (3): 460-462 [Abstract] ( 40 ) HTML (1 KB)  PDF  (0 KB)  ( 31 ) 462 Study on the Relationship Between Pregnancy-associated Plasma Protein A and Ischemic Cere-bral Disease LING Dan-yun%CAO Guo-liang%WA NG Chuan-hui [Objective] To explore the relationship between pregnancy-associated plasma protein A (PAPP-A) and ischemic cerebral disease .[Methods] A total of 48 patients with transient ischemic attack(TIA) and 120 patients with ischemic stroke were enrolled in the study .According to infarction size ,patients with ische-mic stroke were divided into small infarction lesion group( n=58) ,middle infarction lesion group( n=32) and large infarction lesion group( n=30) .Serum PAPP-A and intima-media thickness(IMT) in each group were measured and compared .[Results] Serum PAPP-A and IMT in infarction group were obviously higher than those in TIA group( P<0 .05) .Meanwhile ,PAPP-A level was positively correlated with infarction size ( r =0 .345 ,P <0 .05) .[Conclusion] PAPP-A may be an useful biomarker of ischemic stroke in different degrees . 2014 Vol. 31 (3): 462-464 [Abstract] ( 65 ) HTML (1 KB)  PDF  (0 KB)  ( 37 ) 465 Observation on the Efficacy of Transurethral Resection for the Treatment of Female Patients with Overactive Bladder Caused by Bladder Trigone Mucosal Disease [Objective]To explore the efficacy of electroresection for the treatment of female patients with overactive bladder (OAB) caused by bladder mucosa inflammation .[Methods] A total of 70 female patients with OAB caused by bladder mucosa inflammation diagnosed by clinical examination ,cystoscopy and pathology underwent transurethral resection .[Results] The operation time of 70 patients was 18~30 min with the aver-age of 21 .6 ± 5 .7min .The intraoperative bleeding volume was less than 5ml .The indwelling catheter after the operation lasted for 7 days .Compared with before operation ,OABSS score and QOL score one month after operation were improved( P <0 .05) .[Conclusion] Transurethral resection for the treatment of OAB caused by bladder mucosal inflammation has advantages such as less damage ,less blood loss and satisfactory efficacy . 2014 Vol. 31 (3): 465-466 [Abstract] ( 58 ) HTML (1 KB)  PDF  (0 KB)  ( 36 ) 467 Effect of Acute Insulin Treatment on Regulating PI 3K/Akt and ERK Pathway ZHOU Wei%CHEN Xiao-lan%LIU Si-de [Objective] To investigate the effect of acute insulin treatment on the regulation of PI 3K/Akt and ERK pathway and its significance .[Methods] HepG2 cells and 6 BALB /C male mice aged 8 weeks were treated with acute insulin and phosphate buffered saline (PBS) ,respectively .Total protein was extracted from cells and liver tissues in mice . Akt and ERK phosphorylation were examined by using Western blotting .Immunoprecipitation method was used to detect TERT phosphorylation .[Results]Compared with PBS group ,the levels of p-Akt and p-ERK in HepG2 cells and liver tissues of acute insulin treatment group were up-regulated .TERT activity was decreased after inhibiting PI3K/Akt signa-ling pathway .[Conclusion]Acute insulin treatment can activate PI3K/Akt and ERK pathway .Inhibition of PI3K pathway can decrease the activity of TERT ,which may provide the evidence for the diagnosis and treatment of liver cancer . 2014 Vol. 31 (3): 467-470 [Abstract] ( 52 ) HTML (1 KB)  PDF  (0 KB)  ( 28 ) 471 Application of Free Flap in Patients Undergoing Breast Reconstruction LEI Rui%LI Wei-han [Objective] To explore the application methods and efficacy of free flap in breast reconstruction for patients undergoing breast cancer operation .[Methods]A total of 40 inpatients undergoing breast cancer operation in our department from Jan .2009 to Jan .2011 were randomly divided into study group and control group .The study group underwent breast reconstruction by using inferior epigastric perforating artery free flap ,while the control group underwent breast reconstruction by using transferred lower abdominal transverse rectus abdominis myocutaneous flap .The efficacy was compared between two groups .[Results]The recon-structed breasts in the study group showed no obvious atrophy ,and had soft texture ,and the color and shape were close to normal breasts .Abdominal donor sites recovered well ,and no abdominal weak phenomenon oc-curred .The operation time of the study group was 6 .1 ± 0 .7h ,and postoperative average hospitalization time was 7 .2 ± 1 .1d ,and there was no significant difference between the study group and control group ( P >0 .05) .No postoperative abdominal complications such as subcutaneous hematocele in abdominal donor sites occurred in the study group ,and there was significant difference between the study group and control group ( P<0 .05) .[Conclusion] The inferior epigastric perforating artery free flap for breast reconstruction has certain efficacy ,and can reduce the probability of abdominal complications and not affect the treatment of the tumors , and achieve the requirement for chest shaping .Therefore it is worthy of clinical promotion . 2014 Vol. 31 (3): 471-473 [Abstract] ( 73 ) HTML (1 KB)  PDF  (0 KB)  ( 36 ) 474 Study on the Efficacy of Semiconductor Laser Combined with Sanyrene for the Treatment of Phlebitis Caused by Venous Indwelling Needle [Objective] To explore the efficacy of semiconductor laser combined with sanyrene on phlebitis caused by venous indwelling needle .[Methods]A total of 80 patients with phlebitis caused by venous indwell-ing needle were randomly divided into observation group( n =40) and control group ( n =40) .The observa-tion group was given semiconductor laser irradiation combined with sanyrene besmear in skin region of the af-fected area ,and supplemented by massage .The control group was given routine hydropathic compress with 50% magnesium sulfate .The efficacy of two groups 7d after treatment was observed and compared .[Results]The efficacy of observation group was obviously better than that of control group .The excellent rate of obser-vation group was 82 .5% (33/40) ,which was obviously higher than that of control group(70% ,28/40) ,and there was significant difference between two groups ( P <0 .01) .[Conclusion] Semiconductor laser combined with sanyrene For the treatment of phlebitis caused by venous indwelling needle has certain efficacy ,simple operation and good safety ,and is easy to be accepted by patients ,so it is worthy of clinical promotion . 2014 Vol. 31 (3): 474-476 [Abstract] ( 24 ) HTML (1 KB)  PDF  (0 KB)  ( 21 ) 476 Analysis of Current Situation of Blood Glucose Control and Complications in Patients with Type 1 Diabetes Mellitus in Zhongshan City [Objective] To understand the current situation of blood glucose control and chronic complica-tions in patients with type 1 diabetes mellitus(T1DM ) in Zhongshan city .[Methods]Clinical data of T1DM pa-tients in 5 hospitals of Zhongshan city were registered ,collected and analyzed to understand the current situa-tion of blood glucose control assessed by glycosylated hemoglobin (HbA1c) as well as acute and chronic compli-cations .[Results] Totally 168 T1DM patients were registered and investigated .There were 79 males and 89 females .The mean age was (31 .78 ± 13 .16) years old .The patients were mainly young and middle age .The onset age of the disease was (24 .65 ± 12 .32) years old .The duration of the disease was (7 .14 ± 6 .14) years old .Body mass index(BMI) was (20 .22 ± 3 .25)kg/m2 ,and HbA1c was 9 .20 ± 2 .62% .Only 43(25 .59% ) patients reached the age specific target levels ,and 33 (19 .64% ) patients had more than one kind of chronic complications .About 24(14 .29% ) patients were combined with ketosis .[Conclusion] Blood glucose control of many patients with T1DM does not reach the targeted level .The patients have high incidence of chronic complications . 2014 Vol. 31 (3): 476-478 [Abstract] ( 22 ) HTML (1 KB)  PDF  (0 KB)  ( 34 ) 479 Comparative Study of Amisulpride vs . Olanzapine for the Treatment of Schizophrenia [Objective]To explore clinical efficacy and safety of amisulpride in the treatment of schizophre-nia .[Methods] Totally 121 patients with schizophrenia were randomly assigned to amisulpride group ( n=61) and olanzapine group( n=60) .Two groups were treated with amisulpride and olanzapine respectively for 12 weeks .The efficacy and side reactions were evaluated with positive and negative syndrome scale (PANSS) and treatment emergent symptom scale (TESS ) .[Results] After 12 weeks of treatment , the effective rate of amisulpride and olanzapine were 83 .6% and 86 .7% respectively ,but there was no significant difference be-tween two groups( P > 0 .05) .Compared with before treatment ,the total scores of PANSS in amisulpride group and olanzapine group after treatment were significantly decreased [(41 .79 ± 9 .66) vs .(91 .76 ± 13 .06) , (46 .56 ± 9 .88) vs .(94 .83 ± 15 .67)]( P <0 .01) .The negative symptom scores of PANSS in amisulpride group after 12 weeks of treatment were decreased more significant than those in olanzapine group [(9 .61 ± 3 .86) vs .(12 .56 ± 6 .15)]( P <0 .05) .There was no significant difference in the incidence of side reaction between two groups ( P > 0 .05) .[Conclusion]The total efficacy of amisulpride is similar to olanzapine ,. Amisulpride can better improve negative symptoms ,and has mild side reaction and high safety . 2014 Vol. 31 (3): 479-483 [Abstract] ( 23 ) HTML (1 KB)  PDF  (0 KB)  ( 31 ) 481 Efficacy of Dust Mite Sublingual Drops for the Treatment of Children with Allergic Asthma ZHOU Li-ling [Objective] To explore clinical efficacy of dust mite sublingual drops for the treatment of chil-dren with allergic asthma and its impact on lung function indices .[Methods]A total of 125 children with aller-gen skin test-positive in our hospital were randomly divided into observation group ( n=63) and control group ( n = 63) .The control group received conventional anti-allergy treatment ,while the observation group re-ceived dust mite sublingual drops based on the treatment of control group .Clinical efficacy and the changes of lung function indices in two groups were observed and compared .[Results]Dyspnea ,cough ,pulmonary rales and other clinical symptoms in observation group were markedly better than those in control group ,and there were significant differences( P<0 .05) .There was no significant difference in clinical symptom score and lung function indices before treatment between two groups ( P >0 .05) .Compared with before treatment ,clinical symptom score and lung function indices in two groups after treatment were improved ,and the improvement of observation group was better than the control group ,and there was significant difference( P<0 .05) .[Conclu-sion]Dust mite sublingual drops can effectively increase clinical therapeutic effect of allergic asthma in pediat-ric patients and improve lung function ,so it is helpful for the prognosis of pediatric patients . 2014 Vol. 31 (3): 481-483 [Abstract] ( 30 ) HTML (1 KB)  PDF  (0 KB)  ( 36 ) 484 Clinical Value of P Wave Dispersion for Predicting Atrial Fibrillation in Patients with Hypertension and Left Ventricular Hypertrophy ZHA NG Xiao-hui%XU Wen-bo%LIU Yan [Objective] To explore clinical value of P wave dispersion for predicting atrial fibrillation in patients with hypertension and left ventricular hypertrophy .[Methods] Totally 204 patients with hypertension and left ventricular hy-pertrophy in our hospital from April 2010 to March 2013 were chosen .According to the patient's pathological features , 104 patients with atrial fibrillation were regarded as the observation group and 100 patients without atrial fibrillation were regarded as the control group .Maximum P wave duration(Pmax) ,minimum P wave duration(Pmin) and P wave disper-sion(Pd=Pmax -Pmin) in two groups were measured .Electrocardiogram(ECG) and color pulse Doppler ultrasonography indexes such as left ventricular mass index (LVMI) in two groups were compared .[Results] P wave dispersion in obser-vation group and control group were (45 .3 ± 10 .8)ms and (30 .7 ± 11 .0)ms ,and there was significant difference between two groups( P<0 .05) .Pmax value in observation group was obviously higher than that in control group ,and there was significant difference between two groups ( P < 0 .05) .There was significant difference in LVMI value between two groups[(152 .4 ± 14 .9)g/m2 vs (101 .1 ± 9 .2)g/m2 ]( P<0 .05) .[Conclusion]P wave dispersion and Pmax are the pre-dictive factors of atrial fibrillation in patients with hypertension and left ventricular hypertrophy .It provides critical value reference for the diagnosis of atrial fibrillation in patients with hypertension and left ventricular hypertrophy . 2014 Vol. 31 (3): 484-486 [Abstract] ( 26 ) HTML (1 KB)  PDF  (0 KB)  ( 27 ) 486 Analysis of Clinical Features of Patients with Acute Abdomen During Pregnancy XIAO Ju%FU Bin%LIU Yu-ren [Objective] To explore the clinical features and treatment methods of patients with acute abdo-men during pregnancy .[Methods] Totally 100 cases of pregnancy complicated with acute abdomen in our hos-pital from Oct .2010 to Oct .2012 were selected .Clinical data were analyzed .[Results] Of 100 patients with acute abdomen during pregnancy ,44 patients were acute appendicitis (44% ) ,and 24 patients were cholelithia-sis or acute cholecystitis(24% ) ,and 16 patients were urinary tract stones (16% ) and 8 patients were acute gastroenteritis(8% ) ,and 8 patients were ovarian cyst torsion (8% ) .Among them ,52 patients underwent the operation(52% ) and 48 patients underwent conservative treatment (48% ) .Spontaneous abortion occurred in 4 patients ,and premature delivery occurred in 4 patients .Sixteen patients underwent the surgical treatment and caesarean operation concurrently ,and 76 patients continued pregnancy .No maternal death was found .[Con-clusion]Clinical symptoms of patients with acute abdomen during pregnancy are complex and changeable ,and easily misdiagnosed .The disease condition should be highly paid attention by clinicians .According to auxiliary examination and clinical manifestations ,correct diagnosis for patients should be promptly made to formulate the corresponding treatment measures in order to reduce maternal mortality . 2014 Vol. 31 (3): 486-488 [Abstract] ( 28 ) HTML (1 KB)  PDF  (0 KB)  ( 45 ) 489 Analysis of Drug Resistance of Escherichia Coli in 208 Cases of Urinary Tract Infection LIA NG Jian%ZENG Ling%LI Jiang [Objective] To explore the detection rate and drug resistance of Escherichia coli in urinary tract infection of our region in order to provide the evidence for clinical rational administration .[Methods]The drug susceptibility results of 208 strains of Escherichia coli and extended-spectrum beta-lactamases(ESBLs) isolated from medistream urine of inpatients with urinary tract infection in our hospital between Jan .2010 and Dec . 2011 were analyzed .[Results] The 208 strains of Escherichia coli were isolated and identified .Among them , 109 strains were producing-ESBLs(52 .4% ) .The sensitivity of producing-ESBLs from Escherichia coli to Mer-openem ,imipenem ,Cefoperazone/sulbactam ,Amikacin ,piperacillin/tazobactam and cefoxitin were higher , and the drug resistance rate of producing-ESBLs strains from Escherichia coli was obviously higher than that of non-ESBLs strains .[Conclusion] The drug resistance of Escherichia coli in urinary tract infection is high ,es-pecially for producing ESBLs strains .Strengthening the monitoring of producing ESBLs strains is of important significance for clinical medication and the controlling of the spread of resistant strains . 2014 Vol. 31 (3): 489-494 [Abstract] ( 44 ) HTML (1 KB)  PDF  (0 KB)  ( 39 ) 491 Expression of IL-24 and VEGF-C in Cervical Squamous Carcinoma and Its Clinical Significance LIU Fang%WU Yi-lin [Objective]To explore the expression of interleukin-24(IL-24) and vascular endothelial growth fac-tor-C(VEGF-C) in invasive cervical squamous carcinoma(CSC) and its significance .[Methods]Immunohistochemi-cal method was used to detect the expression of IL-24 and VEGF-C in normal cervix(group A) ,cervical intraepi-thelial neoplasia(CIN ,group B) and invasive cervical squamous carcinoma(group C) .The significance was ana-lyzed .[Results] The expression rate of IL-24 in group A was markedly higher than that in group B and group C , and that in group B was also markedly higher than that in group C .The expression rate of VEGF-C in group A was markedly lower than that in group B and group C ,and that in group B was markedly lower than that in group B ,and there was significant difference( P<0 .05) .The expression of IL-24 in CSC was decreased with the devel-opment of clinical stage ,but was not correlated with age ,pathological stage and interstitial invasion depth ( P>0 . 05) .The expression of IL-24 in cervical cancer group without lymph node metastasis was markedly higher than that in cervical cancer group with lymph node metastasis ( P <0 .05) .There was no significant difference in the expression of VEGF-C in different clinical stage and pathological grade of CSC ( P >0 .05) ,but the expression of VEGF-C in lymph node metastasis group was markedly higher than that in no lymph node metastasis group ( P<0 .05) .The expression of IL-24 in invasive CSC group was negatively related with VEGF-C( P<0 .05) .[Conclu-sion]The expression of IL-24 in normal cervix tissue ,CIN and CSC is gradually decreased ,and it is negative in CSC group with lymph node metastasis .The expression of VEGF-C in CSC is increased gradually and higher in CSC group with lymph node metastasis .There is obviously negative correlation between IL-24 and VEGF-C .It suggests that IL-24 and VEGF-C may be associated with the development and lymph node metastasis of CSC . 2014 Vol. 31 (3): 491-494 [Abstract] ( 59 ) HTML (1 KB)  PDF  (0 KB)  ( 39 ) 495 Clinical Efficacy and Safety of Escitalopram Citalopram Combined with Olanzapine for the Treatment of Senile Schizophrenia with Depression [Objective] To explore clinical efficacy and safety of escitalopram citalopram combined with olanzapine for the treatment of senile schizophrenia with depression .[Methods] Eighty elderly patients with schizophrenia and depression were randomly divided into control group and observation group .The observation group( n=40) was given escitalopram citalopram combined with olanzapine treatment ,while the control group ( n = 40) was given olanzapine treatment .The treatment lasted for 12 weeks .Hamilton depression scale (HAMD) and treatment emergent symptom scale(TESS) was used to assess before and 4 ,8 and 12 weeks af-ter treatment .[Results]There was no significant difference in HAMD scores between two groups before treat-ment( P >0 .05) .HAMD scores of observation group and control group 4 ,8 and 12 weeks after treatment were lower than those before treatment ( P < 0 .05) .HAMD scores of observation group were lower than those of control group at 4 ,8 and 12 weeks(P<0 .05) .The effective rate of observation group was higher than that of control group(85 .0% vs .60 .0% ,P<0 .05) .There was no significant difference in the incidence of adverse reactions between two groups ( P > 0 .05) .[Conclusion]Escitalopram citalopram combined with olanzapine for the treatment of senile schizophrenia with depression has good efficacy and high safety . 2014 Vol. 31 (3): 495-497 [Abstract] ( 38 ) HTML (1 KB)  PDF  (0 KB)  ( 30 ) 497 Observation on the Efficacy of Biofeedback Electric Stimulation Combined with Vaginal Dumb-bell for Pelvic Function Recovery of Patients after Hysterectomy LUO Ping [Objective]To explore the efficacy of biofeedback electric stimulation combined with vaginal dumbbell for pelvic function recovery of patients after hysterectomy .[Methods] Totally 160 patients after open abdominal hysterectomy were divided into 4 groups with 40 patients in each group .Group A received ke-gel training .Group B received vaginal dumbbell training .Group C received biofeedback electric stimulation therapy .Group D received biofeedback electric stimulation combined with vaginal dumbbell training .Pelvic muscle strength in 4 groups was compared before and after treatment .[Results] There was significant differ-ence in pelvic muscle strength between group D and other 3 groups( P<0 .05) .[Conclusion]Biofeedback e-lectrical stimulation combined with vaginal dumbbell can effectively improve pelvic function of patients after hysterectomy . 2014 Vol. 31 (3): 497-499 [Abstract] ( 69 ) HTML (1 KB)  PDF  (0 KB)  ( 36 ) 500 Observation on the Efficacy of Tiotropium Combined with Seretide for the Treatment of Elderly Patients with Moderate to Severe COPD CHENG Li-xin [Objective] To explore clinical efficacy of tiotropium combined with seretide for the treatment of elderly patients with severe chronic obstructive pulmonary disease (COPD) .[Methods] Totally 46 elderly patients with moderate to severe COPD were randomly divided into experimental group and control group with 23 patients in each group .Patients received tiotropium combined with seretide (experimental group) and simple seretide respectively on the basis of conventional therapy .Clinical symptom score ,lung function and arterial blood gas analysis changes in two groups 6 month after treatment were observed and compared .[Results]Compared with before treatment ,clinical symptom score of both groups after treatment decreased significant-ly ,while lung function and arterial blood gas analysis improved significantly ,and there were significant differ-ences between before and after treatment ( P <0 .05) .After treatment ,clinical symptom score ,lung function and arterial blood gas analysis of experimental group improved more obvious than the control group ,and there was significant difference between two groups ( P <0 .05) .[Conclusion] Tiotropium combined with seretide for the treatment of elderly patients with moderate to severe COPD has satisfactory efficacy and high safety ,so it is worthy of clinical application . 2014 Vol. 31 (3): 500-502 [Abstract] ( 32 ) HTML (1 KB)  PDF  (0 KB)  ( 36 ) 502 Observation on the Efficacy of Three Cavity Nasal Jejunal Feeding Tube Nursing for the Recov-ery of Nutrition Status in Patients with Total Gastrectomy WANG Xiao-bin [Objective]To explore the efficacy of three cavity nasal jejunal feeding tube nursing (TCNJ-FTN) for the recovery of nutrition status in patients with total gastrectomy .[Methods]A total of 86 patients with total gastrectomy were chosen .Among them ,43 patients treated with indwelling stomach tube and rou-tine care were selected as control group ,and 43 patients treated with TCNJ-FTN were selected as observation group .The recovery status after operation ,wound healing time ,hospitalization time and nursing satisfaction were compared between two groups .[Results] The postoperative recovery in observation group was markedly better than the control group ,and there was significant difference ( P < 0 .05) .The average wound healing time and hospitalization time of observation group were markedly less than those of control group ,and there was significant difference( P<0 .05) .Nursing satisfaction rate of observation group was markedly higher than that of control group ,and there was significant difference( P <0 .05) .[Conclusion] For patients with total gastrectomy ,TCNJ-FTN treatment and care can promote the rehabilitation of patients and increase nursing satisfaction .It is worthy of clinical promotion . 2014 Vol. 31 (3): 502-504 [Abstract] ( 50 ) HTML (1 KB)  PDF  (0 KB)  ( 39 ) 505 Comparison of the Efficacy of Different Methods for Relieving Cystospasm after Transurethral Prostatic Resection [Objective] To observe the efficacy of patient-controlled intravenous analgesia (PCA ) ,succinic acid solifenacin tablet and tolterodine tartrate sustained release tablet combined with PCA for the treatment of cystospasm after transurethral prostatic resection(TURP) .[Methods] Totally 80 patients after TURP in our hospital were ran-domly divided into 4 groups .The control group was given pethidine 50mg and anisodamine 10mg by muscle injection . PCA group was given continuous venous injection with sufentanil 100μg and granisetron 6mg by vein analgesia pump , which were diluted to 100mL with normal saline ,and the dosage was adjusted into 2mL by constant speed for 72h . Solifenacin group was given oral succinic acid solifenacin tablet 5mg for once a day .The combination group was given oral tolterodine tartrate sustained release tablet 2mg for once a day and PCA therapy .Clinical efficacy was compared among groups .[Results]The frequency and mean duration of cystospasm and bladder washout times in 3 experimental groups d1 ,d2 and d3 after operation were shorter than those in control group .The frequency and mean duration of cystospasm in combination group were shorter than those in PCA group and solifenacin group ,and there was signifi-cant difference among groups (all P<0 .05) .There was no significant difference in all indicators between PCA group and solifenacin group( P>0 .05) .[Conclusion]PCA ,succinic acid solifenacin tablet and tolterodine tartrate sustained release tablet combined with PCA for the prevention and treatment of early cystospasm after TURP have good effica-cy .The combination group has more significant efficacy and should be promoted in clinical practice . 2014 Vol. 31 (3): 505-507 [Abstract] ( 83 ) HTML (1 KB)  PDF  (0 KB)  ( 31 ) 507 Effect of Comprehensive Sleep Nursing Care on Sleep Quality of Inpatients with Asthma YA NG Yu%WA NG Na%ZHA NG Jun-li [Objective]To explore the effect of comprehensive sleep nursing care on sleep quality of inpa-tients with asthma .[Methods]According to the order of admission ,216 inpatients with asthma from Oct . 2011 to Nov .2013 were involved in this research and were divided into control group and intervention group . The control group( n =110) was given conventional nursing care ,while the intervention group( n=106) was given comprehensive sleep nursing care including changing sleep environment ,developing good sleep habits , rationally using drugs and implementing psychological care based on conventional nursing care . Pittsburgh sleep quality index scale(PSQI) was used to evaluate sleep quality 1~2d after admission and at the discharged day .[Results]Compared with the admission ,the PSQI score in intervention group at the discharged day was markedly decreased[(6 .87 ± 1 .27) vs .(9 .01 ± 1 .73)] and obviously lower than those in control group ,and there was significant difference( P<0 .01) .There was no significant difference in PSQI score of control group between before and after nursing care( P>0 .05) .[Conclusion]Comprehensive sleep nursing care can improve the sleep quality of inpatients with asthma . 2014 Vol. 31 (3): 507-509 [Abstract] ( 40 ) HTML (1 KB)  PDF  (0 KB)  ( 36 ) 510 Application of Cervical Plexus Block Combined with General Anesthesia in Carotid Endarterec-tomy [Objective]To observe the application of cervical plexus block combined with general anesthesia in carotid endarterectomy .[Methods] According to anesthesia method ,40 patients undergoing carotid endar-terectomy in our hospital from Jan .2012 to Aug .2013 were randomly divided into control group(G group ,n=20) undergoing general anesthesia group and observation group (GL group ,n = 20) undergoing cervical plexus block combined with general anesthesia .Hemodynamics ,awakening time and the dose of analgesics in two groups were observed and analyzed .Mini-mental state examination(MMSE) was used to observe cognitive function 1d before and 1d ,3d and 6d after operation .[Results] The fluctuation of hemodynamics in GL group at each time point was obviously lower than that in G group ( P<0 .05) ,and the dose of intraoperative vasoac-tive agents was also lower than that in G group( P<0 .05) .The awakening time and the dose of analgesics in G group were markedly more than those in GL group( P<0 .05) .Compared with baseline value before opera-tion ,MMSE score at 1d after operation in two groups was obviously decreased( P<0 .05) ,and MMSE score in G group was lower than that in GL group ,and there was significant difference( P <0 .05) .There was no significant difference between MMSE score in two groups at 3d and 6d after operation and preoperative baseline vale( P > 0 .05) .[Conclusion] Patients undergoing carotid endarterectomy under cervical plexus block com-bined with general anesthesia have stable hemodynamics , short awakening time , little dose of analgesics group ,small impact on neurological function after operation and satisfactory efficacy ,and can obviously im-prove the safety and quality of anesthesia . 2014 Vol. 31 (3): 510-513 [Abstract] ( 70 ) HTML (1 KB)  PDF  (0 KB)  ( 34 ) 513 Preliminary Study on the Indications for Gallbladder-preserving Cholelithotomy NANG Yun-guang%DING Hai-bo%LIU Da-lin [Objective] To explore the correlation of gallbladder contraction dysfunction and cholecystokinin (CCK ) A re-ceptor(AR) with gallbladder stone in order to provide the theoretical basis for identifying the indications of gallbladder-preser-ving cholelithotomy .[Methods]Type-B ultrasound was used to measure gallbladder wall thickness ,length ,width and trans diameter in gallbladder polyps group and stone groups .Gallbladder contraction efficiency was calculated .Enzyme immunohis-tochemical method was used for staining .The expression of CCK-AR in gallbladder wall was observed .[Results]The thick-ness of gallbladder wall in gallbladder stone group was obviously higher than that in gallbladder polyps group ( P <0 .05) . Gallbladder contraction efficiency in gallbladder stone group was obviously lower than that in gallbladder polyps group ( P <0 .05) .There was significantly negative correlation between gallbladder contraction efficiency and the thickness of gallbladder wall in gallbladder stone group .The expression of CCK-AR in gallbladder stone group was lower than that in gallbladder pol-yps group( P<0 .0) .There was significantly positive correlation between gallbladder contraction efficiency and the cell num-ber of positive expression of CCK-AR in smooth muscle of gallbladder stone group .[Conclusion]Gallbladder contraction effi-ciency is associated with the decreasing of CCK-AR .There is negative correlation between gallbladder wall thickness and gall-bladder contraction efficiency in patients with gallbladder stone .The basic conditions for gallbladder-preserving cholelithotomy are gallbladder contraction efficiency>50% and gallbladder wall thickness<0 .30cm . 2014 Vol. 31 (3): 513-516 [Abstract] ( 26 ) HTML (1 KB)  PDF  (0 KB)  ( 34 ) 517 Observation of the Efficacy of Calcium Dobesilate Combined with Olmesartan for the Treatment of Proteinuria in Diabetic Nephropathy [Objective]To explore the clinical efficacy of calcium dobesilate combined with olmesartan for the treatment of proteinuria in diabetic nephropathy (DN) .[Methods]A total of 70 DN patients with proteinu-ria in our hospital from Feb .2012 to April 2013 were randomly divided into observation group ( n =35) and control group( n=35) .The control group received regular treatment including olmesartan and other hypogly-cemic hypotensive treatment .The observation group was additionally given calcium dobesilate based on the treatment of control group .The 24h-urinary albumin excretion rates(UAER) ,monocyte chemotactic protein-1 (MCP-1) and urinary α1-microglobulin(α1-MG) before and 12 weeks after treatment were compared between two groups .[Results] The 24h-UAER in observation group after 12 weeks of treatment was decreased more obviously ,and there was significant difference( P <0 .05) .The total effective rate of observation group was 88% ,which was markedly higher than that of control group(68% ) ,and there was significant difference( P<0 .05) .[Conclusion]Calcium dobesilate combined with olmesartan for the treatment of proteinuria in DN has good efficacy and safety . 2014 Vol. 31 (3): 517-521 [Abstract] ( 81 ) HTML (1 KB)  PDF  (0 KB)  ( 32 ) 519 Self-made Traditional Chinese Medicine Ped-bath for the Treatment of Elderly Patients with Obstructive Atherosclerosis of Lower Limbs LIU Feng-ge%YA NG Wen-ju%DONG Jun-li [Objective]To explore the clinical efficacy of traditional Chinese medicine ped-bath for the treatment of obstructive atherosclerosis of lower limbs .[Methods]Totally 120 patients were randomly divided into treatment group and control group .The control group was given buflomedil 10mg once daily .The treat-ment group was additionally given traditional Chinese medicine ped-bath prescription once daily .The frequen-cy ,duration and degree of pain and ankle brachial index (ABI) before and at the end of 1 and 2 weeks after treatment were observed .[Results] The decreasing of the frequency ,duration and degree of pain ,the im-provement of ABI and the reducing of the recurrence in traditional Chinese medicine group were obviously bet-ter than those in single buflomedil group .[Conclusion] Traditional Chinese medicine ped-bath combined with buflomedil for the treatment of obstructive atherosclerosis of lower limbs has good efficacy .symptom . 2014 Vol. 31 (3): 519-521 [Abstract] ( 57 ) HTML (1 KB)  PDF  (0 KB)  ( 35 ) 522 Clinical Research on the Correlation Between Preoperative Pulmonary Function and the Recov-ery after Lobectomy ZHANG Xue-feng%TANG Zhi [Objective]To evaluate preoperative pulmonary function ,and to explore the correlation be-tween preoperative pulmonary function and the recovery after lobectomy .[Methods] Totally 68 patients under-going lobectomy in our hospital from Jan .2007 to Jan .2012 were chosen .Among them ,48 patients under-went single lobectomy and 20 patients underwent pneumonectomy .According to maximal voluntary ventilation (MVV) and forced expiratory volume in one second(FEV1) ,preoperative pulmonary function was divided into good ,medium and poor .Postoperative recovery time (inhaling oxygen ,ICU and extubation) was discussed and compared .The corresponding statistical analysis was taken .[Results] The most of patients were satisfied with the operation efficacy .Postoperative efficacy was good .There were significant differences in preoperative pulmonary function and postoperative recovery time ( P <0 .05) .For different surgical resection extent ,there was no significant difference in few analysis .But there were some different trends .[Conclusion] Preoperative pulmonary function evaluation is closely related to the recovery status after lobectomy .The recovery time of patients with better preoperative pulmonary function after lobectomy is shorter . 2014 Vol. 31 (3): 522-526 [Abstract] ( 37 ) HTML (1 KB)  PDF  (0 KB)  ( 35 ) 524 Role of TCT and Electronic Colposcopy in the Diagnosis of High -level Cervical Intraepithelial Neoplasia LI Bi-rong%LI Jie%WEI Ying-ying [Objective] To evaluate the value of thinprep cytologic test (TCT ) ,electronic colposcopy and colposcopically directed biopsy pathology in the diagnosis of high - level cervical intraepithelial neoplasia (CIN) .[Methods]A total of 247 patients with high squamous intraepithelial neoplasia(HSIL) diagnosed by TCT in our hospital from Jan .2009 to May 2013 received electronic colposcopy ,and were diagnosed by ima-ges .Cervical biopsies were performed for pathological examination .The results of TCT were compared with the results from electronic coloposcopy and colposcopically directed biopsy pathology .The pathology of 156 cases by colposcopy was compared with the pathology by paraffin section .[Results]Among 247 cases of HSIL by TCT ,221 cases(89 .5% ) were high -level CIN (CINⅡ and CINⅢ ) diagnosed by colposcopically directed biopsy pathology ,and 5 cases(2 .0% ) were infiltrating carcinoma .The sensitivity and positive predictive value of electronic colposcopy for the diagnosis of cervical disease were 98 .7% (231/234) and 95 .9% (231/241) ,re-spectively .There was no significant difference between colposcopy inspection and colposcopically directed bi-opsy pathology( P>0 .05) .There was significant difference between colposcopically directed biopsy pathology and postoperative paraffin section pathology ( P<0 .05) .[Conclusion] TCT is an effective method for screen-ing high-level CIN .Electronic colposcope is an important and reliable clinical examination method for the auxiliary diagnosis of high -level CIN .Typical abnormal cervical images under colposcopy have a critical diag-nostic value in high -level CIN and cervical cancer .Single colposcopically directed biopsy pathology can not take place of postoperative pathological diagnosis . 2014 Vol. 31 (3): 524-526 [Abstract] ( 29 ) HTML (1 KB)  PDF  (0 KB)  ( 35 ) 527 Determination and Significance of Serum TNF-α and NF-κB in Patients with Essential Hyper-tension JIANG Chao-hui%WANG Jian-jun%CHEN Wen-min [Objective]To explore the changes and clinical significance of serum tumor necrosis factor-α(TNF-α) and nuclear factor-κB(NF-κB) in patients with essential hypertension (EH) .[Methods] Patients with gradeⅠ( n=30) ,Ⅱ(n=30) and Ⅲ( n=30) EH were selected .Other 30 healthy patients were selected as the controls .Enzyme-linked immunosorbent assay (ELISA) was used to detect serum levels of TNF-αand NF-κB .[Results]Serum levels of TNF-αand NF-κB in EH group were obviously higher than those in normal group ( P<0 .01) .There was significant difference in serum levels of TNF-αand NF-κB among patients with different grade of EH ( P<0 .05 or P<0 .01) . Serum levels of TNF-αand NF-κB increased with the exacerbation of EH .Serum levels of TNF-αin EH group and EH groups with different grade were positively correlated with NF-κB( P<0 .05) .[Conclusion] TNF-αand NF-κB may be involved in the incidence and progress of EH .The monitoring of serum TNF-αand NF-κB levels can be used as the indicators for assessing the condition of EH . 2014 Vol. 31 (3): 527-529 [Abstract] ( 30 ) HTML (1 KB)  PDF  (0 KB)  ( 33 ) 529 Analysis of the Incidence and Risk Factors of Depression in Women after Breast Cancer Surgery WANG Li-xin%XUE Feng [Objective]To explore the incidence and risk factors of postoperative depression for breast cancer in women .[Methods] Totally 486 patients undergoing breast cancer surgery in breast surgery department of our hospi-tal from April 2000 to April 2013 were chosen .The incidence of depression after breast cancer surgery in women was discussed .The related factors of postoperative depression were analyzed .[Results]Of 486 patients undergoing breast cancer surgery ,184 patients had postoperative depression ,and the incidence rate of depression was 37 .86% .There was significant difference in age and personal income between depression patients and non-depression patients after breast cancer surgery ( P<0 .05) .Clinical data such as postoperative time ,surgical method and clinical stage in de-pression patients after breast cancer surgery were similar to those in non-depression patients ,and there was no signifi-cant difference between groups .[Conclusion] The postoperative depression in breast cancer has high incidence rate , which is correlated with general social characteristics such as age and income ,but is not obviously correlated with clin-ical characteristics of patients .Mastering the related risk factors of depression after breast cancer surgery can contrib-ute to the targeted intervention to reduce the incidence of postoperative depression ,improve the quality of life of pa-tients and promote the prognosis . 2014 Vol. 31 (3): 529-531 [Abstract] ( 32 ) HTML (1 KB)  PDF  (0 KB)  ( 33 ) 532 Investigation and Countermeasures of Health Quotient of Inpatients with Type 2 Diabetes Mellitus on Admission Day XIAO Shi-qi%XUE Peng-shi [Objective]To investigate the situation of health quotient of inpatients with type 2 diabetes mellitus (T2DM ) on admission day and put forward the countermeasures .[Methods]Health quotient questionnaires and the common situation survey scales were given out in 140 inpatients with T2DM in our hospital on admission day . The situation of health quotient was analyzed by using SPSS 11 .5 statistical software .[Results] The scores of health knowledge among the factors of health quotient questionnaires in 140 T2DM patients on admission day were the lowest ,which were alarming .The method of acquiring physical and mental health was obtained from the scores of all dimensions of health quotient questionnaire .The scores of 5 dimensions including consciousness of physical and emotional state ,knowledge of health maintenance ,health risk factors and knowledge of health moni-toring tools ,smoking ,drinking and drug taking were the lowest ,which were alarming .[Conclusion]The alarming health quotient factors and dimensions of inpatients with T 2DM on admission day should be focused on in order to better carry out health education guided by the patients'needs . 2014 Vol. 31 (3): 532-534 [Abstract] ( 63 ) HTML (1 KB)  PDF  (0 KB)  ( 34 ) 534 Effect of Serum Interleukin-6 on Atherosclerosis of Patients with Impaired Glucose Tolerance JI Hong-mei [Objective] To explore the relationship of carotid intima-media thickness(IMT) with serum inter-leukin-6(IL-6) in patients with impaired glucose tolerance(IGT) .[Methods] According to carotid IMT ,297 IGT patients were divided into normal IMT group(n=103 ,IMT<1 .0mm) ,IMT thickening group( n=99 ,1 .0mm 1 .3mm) .Other 100 healthy subjects were chosen as control group .Serum IL-6 was determined .[Results] Serum IL-6 in IGT group was higher than that in control group(p<0 .01) ,but there was no significant difference in IL-6 between normal IMT group and control group( P>0 .05) .In IGT group ,serum IL-6 in IM T thickening patients was markedly higher than that in normal IMT group( P <0 .01) .The positive correlation between IL-6 and IMT was shown in both IMT thickening group and atherosclerotic plaque group(r was 0 .567 and 0 .682 respectively ,P<0 .01) .[Conclusion] Serum IL-6 in IGT patients may have some correlation with early atherosclerosis . 2014 Vol. 31 (3): 534-536 [Abstract] ( 46 ) HTML (1 KB)  PDF  (0 KB)  ( 23 ) 537 Level of H2O2, T-Aoc and 25(OH)D3 in Obese Men with Normal Glucose Tolerance and Its Re-lationship with Insulin Resistance [Objective] To observe the levels of hydrogen peroxide (H2 O2 ) ,total antioxidant ability (T-Aoc) and 25(OH)D3 in obese neb with normal glucose tolerance(NGT) and its relationship with insulin resist-ance .[Methods] Totally 86 young men(aged 20~45 years old) with NGT were enrolled in the study ,and di-vided into obese group( n=44 ,BMI≥25kg/m2 ) and normal control group( n=42 ,BMI<25kg/m2 ) .H2 O2 , T-Aoc and 25(OH)D3 were measured .The risk factors of insulin resistance with insulin resistance index (Ho-ma-IR) as dependent variable were analyzed by using multiple linear regression .[Results] Diastolic blood pressure(DBP) ,triglyceride(TG) ,Fasting insulin(FINS) ,insulin after 2h-sugar load(2hINS) ,H2O2 and Homa-IR in obese group were higher than those in control group ( P <0 .05) ,while high density lipoprotein (HDL) ,T-Aoc and 25(OH)D3 were lower than those in control group( P<0 .05) .T-Aoc showed a negative correlation with BMI( r = -0 .321) ,DBP( r = -0 .330) and Homa-IR( r = -0 .342)( P < 0 .05) ,and a positivecorrelationwithHDL(r =0.484)and25(OH)D3(r =0.312)(P <0.05).H2O2 showedapositive correlation with BMI( r =0 .302) and Homa-IR( r =0 .381)( P <0 .05) .Multiple linear regression showed that the correlation of 25(OH)D3 and T-AOC with Homa-IR was the strongest .[Conclusion] Obese young men with NGT have the metabolic disorder of blood lipid and blood pressure ,insulin resistance ,vitamin D de-creasing and oxidative stress increasing .Oxidative stress and vitamin D are the important risk factors of insulin resistance . 2014 Vol. 31 (3): 537-540 [Abstract] ( 18 ) HTML (1 KB)  PDF  (0 KB)  ( 28 ) 540 Detection and Analysis of Leukotriene E4 and Tumor Necrosis Factor of Patients with Seasonal Allergic Rhinitis [Objective] To explore changes and clinical significance of leukotriene E 4(LTE4) and tumor necrosis factor-alpha(TNF-α) in seasonal allergic rhinitis(SAR) .[Methods] Totally 40 patients who met the SAR diagnosis standard were regarded as observation group ,while 40 healthy people were regarded as control group .Serum levels of LTE4 and TNF-αin each season were detected .[Results]Compared with control group ,serum levels of LTE4 and TNF-αof observation group in spring and winter were increased significantly (LTE4:P <0 .05 in spring and P <0 .01 in winter)(TNF-α:all P <0 .01 in spring and winter) .There was no significant difference in serum levels of LTE4 and TNF-αin summer and autumn between two groups ( P>0 .05) .Serum LTE4 had no obvious correlation with TNF-α.[Conclusion] Serum levels of LTE4 and TNF-α play an important role in the pathogenesis of SAR through local inflammatory response .They are of great reference value in clinical prevention and treatment . 2014 Vol. 31 (3): 540-542 [Abstract] ( 32 ) HTML (1 KB)  PDF  (0 KB)  ( 30 ) 542 Study on First Ambulation Status of Patients with Colorectal Cancer After Operation WANG Ping [Objective] To explore the status of first ambulation of patients with colorectal cancer ,and to analyze the related factors .[Methods]Totally 100 patients undergoing elective surgery for colorectal cancer were enrolled in the stud-y .According to the accomplishment status of early ambulation ,all patients were divided into accomplishment group and non-accomplishment group .General condition questionnaire ,the log of postoperative early ambulation ,pain self-rating scale and specific condition questionnaire were used to observe and analyze first ambulation status .[Results] Among all patients ,82 patients completed first ambulation and 28 patients did not complete . .The time of first postoperative ambu-lation of all patients after operation was (76 .01 ± 11 .24)h ,and the distance of first walk was 38m .There was no signifi-cant difference in sex ratio and age ratio ( P>0 .05) .There was significant difference in operative site and average time of initial ambulation .Statistical analysis showed that dizziness and pain intensity had greater impact on initial ambulation .[Conclusion]When the patients with colorectal cancer do the initial ambulation after operation ,wound pain and dizziness should be closely observed to ensure the safety of patients . 2014 Vol. 31 (3): 542-544 [Abstract] ( 34 ) HTML (1 KB)  PDF  (0 KB)  ( 32 )

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