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Abstract [Objective]To explore the effect of C-type natriuretic peptide (CNP ) on antagonizing 5-hydroxytryptamine(5-HT)-induced aortic smooth muscle cell contraction and its mechanism .[Methods]CNP stimulated A10 aortic smooth muscle cells in vitro by different concentrations (10-5M-10-9M ,respectively ) at different time points(0 ,5 ,10 ,15 ,20 and 30 minutes) .Then enzyme-linked immunosorbent assay(ELISA) was used to detect the changes of cGMP concentration .The expressions of PKGIα and PKGIβ in cells after CNP stimulation were detected by Western blot .The effect of CNP on 5-HT-induced cell contraction was ob-served by confocal microscopy .[Results] The generation of cGMP in smooth muscle cells after CNP treatment was obviously increased in a dose dependent manner ,and markedly increased in the beginning (5min) and reached to the peak around 10min .Meanwhile ,the expression of cGMP downstream molecule ,cGMP-depend-ent protein kinase (PKG ) after CNP treatment was activated which presented the increased expression of PKGIα.After PKG specific inhibitor interfered ,the antagonistic effect of CNP on 5-HT-induced aortic vascu-lar smooth muscle cell contraction was decreased .[Conclusion]CNP can activate cGMP-PKGIa signal against 5-HT-induced aortic vascular smooth muscle cell contraction .It indicates that CNP can be used as an effective target for the prevention and treatment of atherosclerosis .
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2014, Vol. 31 
