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Abstract [Objective] To investigate the effect of acute insulin treatment on the regulation of PI 3K/Akt and ERK pathway and its significance .[Methods] HepG2 cells and 6 BALB /C male mice aged 8 weeks were treated with acute insulin and phosphate buffered saline (PBS) ,respectively .Total protein was extracted from cells and liver tissues in mice . Akt and ERK phosphorylation were examined by using Western blotting .Immunoprecipitation method was used to detect TERT phosphorylation .[Results]Compared with PBS group ,the levels of p-Akt and p-ERK in HepG2 cells and liver tissues of acute insulin treatment group were up-regulated .TERT activity was decreased after inhibiting PI3K/Akt signa-ling pathway .[Conclusion]Acute insulin treatment can activate PI3K/Akt and ERK pathway .Inhibition of PI3K pathway can decrease the activity of TERT ,which may provide the evidence for the diagnosis and treatment of liver cancer .
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2014, Vol. 31 
