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Quick Search     Adv Search 2016 Vol. 33, No. 10 Published: 2016-10-26 Article          Article 1873 Epidemiological Investigation of HPV Infections and Subtype Analysis of 6,617 Female Cases in Wuhan Area from 2011 to 2015 QIAO Bin, LI Yan, WANG Ming,et al DOI: 10.3969/j.issn.1671-7171.2016.10.001 【Objective】To investigate the epidemic and subtype distribution of human papilloma virus (HPV) of female outpatients in Wuhan in order to provide guidance for prevention and treatment of HPV infection. 【Methods】 HPV infection and typing were detected by DNA molecular rapid diversion hybridization gene chip technology, and the positive or negative and genotype were judged according to the location of the subtype distribution in the chip.【Results】 HPV typing detection was performed in 6,617 patients, and 1,856 of them were positive for HPV, and the positive rate was 28.05%; 1,856 patients were infected with HPV subtype 2,543 times, The most common types of infection were HPV16 (30.28%), HPV52 (18.75%), HPV58 (15.03%), HPV18 (8.78%) and HPV33 (8.51%) ; Among them, the infection rate of the high-risk combined with low-risk subtype was 91.11%, and increased with age. HPV infected women mainly concentrated in the age of 31~50 years old, accounting for 66.16%; the highest positive rate was among those younger or equivalent to 20 years old, reached 35.05%; The single subtype of HPV infection took the majority , accounting for 73.06%. Among the multiple infections, the dual infection played a main role, and the common model for dual infection were HPV16+HPV52 and HPV16+HPV58.【Conclusion】 The HPV infection rate of female outpatients in Wuhan is rather high, and the population is concentrated on 31~50 years old. The main infection genotypes include high risk subtypes like HPV16, HPV52, HPV58, HPV18 and HPV33. 2016 Vol. 33 (10): 1873-1876 [Abstract] ( 124 ) HTML (1 KB)  PDF (1232 KB)  ( 84 ) 1876 Expression and Significance of FHIT and Survivin in Tissues of Sinonasal Inverted Papilloma JING Yang, YANG Xiao-rong, WANG Yu-juan,et al DOI: 10.3969/j.issn.1671-7171.2016.10.002 【Objective】To detect and analyze the expression level and significance of fragile histidine three (FHIT) and Survivin in the tissues of sinonasal inverted papilloma (SNIP) .【Methods】 Fifty-eight cases of tissue samples of SNIP (SNIP group) , 20 cases of tissue samples of squamous cell carcinoma in the nasal cavity and nasal sinuses (Squamous Cell Carcinoma group) and 20 cases of normal nasal mucosa specimens (normal group) from Jan.- Dec.2015 in our hospital were collected. The expression levels of FHIT and Survivin in the three groups were observed by immunohistochemical method, and the clinical pathological characteristics of the patients with SNIP were analyzed.【Results】The positive expression rate of FHIT protein in the SNIP group was significantly lower than that in the normal group (P<0.05), and was higher than that in the squamous cell carcinoma group (P<0.05); the positive expression rate of Survivin protein in the SNIP group was significantly higher than that in the normal group (P<0.05); the positive expression rate of FHIT protein and the positive rate of Survivin protein in the SNIP group were significantly correlated with the clinical stage of the SNIP group (P<0.05).【Conclusion】Downregulation of FHIT protein expression and upregulation of Survivin protein play an important role in the occurrence and development of SNIP. 2016 Vol. 33 (10): 1876-1879 [Abstract] ( 104 ) HTML (1 KB)  PDF (1880 KB)  ( 73 ) 1880 Effect of Ghrelin on the Expression of Breast Cancer Resistance Protein (BCRP) in Breast Cancer Cells QU Wen-Zhi, LI Zi-Hao, ZHANG Yun-Wei,et al DOI: 10.3969/j.issn.1671-7171.2016.10.003 【Objective】 To study the effects of ghrelin on the expression of breast cancer resistance protein(BCRP) in breast cancer cell line. 【Methods】Human breast cancer cell line MDA-MB-231 were cultured and treated with ghrelin, doxorubicin, and ghrelin combined with doxorubicin. The tunnel method was used to detect cell apoptosis. The Western-blot method was used to detect p-P38, P38, and BCRP protein levels. 【Results】Tunnel assay showed that under the addition of ghrelin, the human breast cancer cell MDA-MB-231 proliferated and the apoptosis rate was significantly lower than when doxorubicin was used. Ghrelin combined with doxorubicin inhibits the killing effect of doxorubicin on the human breast cancer cell line MDA-MB-231(P<0.001). The P38 and p-P38 protein levels increased in the Ghrelin Group, and the protein expression of BCRP increased significantly as well. The P38 and p-P38 protein levels and BCRP protein expression decreased in the Ghrelin combined with doxorubicin group, but the decrease was less compared to the doxorubicin group (P<0.05). 【Conclusion】The P38MAPK signaling pathway activated by ghrelin promotes breast cancer cell BCRP expression, inhibiting the killing effects of doxorubicin-induced apoptosis of breast cancer cells. 2016 Vol. 33 (10): 1880-1882 [Abstract] ( 74 ) HTML (1 KB)  PDF (1237 KB)  ( 96 ) 1883 Drug Resistance Monitoring Analysis of Pulmonary Tuberculosis Complicated with Lung Cancer HAN Na, ZHAO Lei, WANG Li, et al DOI: 10.3969/j.issn.1671-7171.2016.10.004 【Objective】To investigate drug resistance monitoring in 72 cases of pulmonary tuberculosis complicated with lung cancer in our hospital. 【Methods】Seventy-two patients with pulmonary tuberculosis complicated with lung cancer were included in the pulmonary tuberculosis with lung cancer group. Another 94 patients with simple pulmonary tuberculosis admitted in the same period were included in the pulmonary tuberculosis group. The drug resistances of Mycobacterium tuberculosis to isoniazid (INH), rifampicin (RFP), streptomycin (SM) and ethambutol (EMB) were analyzed. 【Results】The acquired drug resistance rate of pulmonary tuberculosis with lung cancer group (89.47%) was significantly higher than that of the pulmonary tuberculosis group (P<0.05). The probabilities of resistance to RFP and EMB in the pulmonary tuberculosis with lung cancer group were significantly higher than that in the pulmonary tuberculosis group (P<0.05). The probability of multi-drug resistance in the pulmonary tuberculosis with lung cancer group (71.93%) was significantly higher than that in the pulmonary tuberculosis group (39.04%) (P<0.05). 【Conclusion】Compared to simple pulmonary tuberculosis, the acquired drug resistance rate, multi-drug resistance rate, and frequency of resistance to RFB and EMB of pulmonary tuberculosis with lung cancer are higher. 2016 Vol. 33 (10): 1883-1885 [Abstract] ( 87 ) HTML (1 KB)  PDF (1205 KB)  ( 60 ) 1885 Application value of Five-transverse -section Echocardiography Ultrasound in Screening of Fetal Cardiac Malformation SUN Dong-yan, DONG Ming-hua, XIAO Ying ,et al DOI: 10.3969/j.issn.1671-7171.2016.10.005 【Objective】 To study the clinical value of five-transverse -section echocardiography ultrasound in fetal heart malformation screening.【Methods】Eight thousand one hundred and fifty-six cases of pregnant women in our hospital from January 2011 to June 2014 were selected as the research objects, the detection of five-transverse-section of the fetus heart (abdominal transverse section, heart four chamber view, left ventricular outflow tract, right ventricular outflow tract, three blood vessels - trachea view) was conducted to compare the results with those of the autopsy and the echocardiography of the newborn infants, and to analyze the abnormal karyotype . 【Results】 8,156 cases of pregnant women were successfully completed the heart five-transverse-section ultrasound examination; Congenital heart defects were detected in 43 cases, the detection rate was 0.53%; Autopsy or postnatal echocardiography of the newborn infants showed 5 cases of missed diagnosis and 3 cases of misdiagnosis, among which 2 cases of the inner cushion defect were misdiagnosed as simple ventricular septal defect(ASD), and 1 case of tetralogy of Fallot misdiagnosed as simple atrial septal defect (ASD). The specificity, sensitivity, negative predictive value and positive predictive value of the five -transverse-section Ultrasound heart examination were 99.96%(8088/8091), 88.89%(40/45), 99.93%(8088/8093) and 93.02%(40/43) respectively. Within 43 cases of fetal heart malformation, 19 cases performed chromosome karyotype examination, and abnormal karyotype was found in 9 cases.【Conclusion】 The routine prenatal heart five-transverse-section Ultrasound examination can be used to screen for fetal congenital cardiac malformation more accurately, and it is conducive to infer the fetal chromosomal abnormalities. 2016 Vol. 33 (10): 1885-1887 [Abstract] ( 105 ) HTML (1 KB)  PDF (1604 KB)  ( 77 ) 1888 Effect of Whole Brain Radiotherapy on Cognitive Function and Prospective Memory in Patients with Brain Metastases HU Chan-chan, LIAN Xiang-rao, WANG Rui DOI: 10.3969/j.issn.1671-7171.2016.10.006 【Objective】 To explore the effect of whole brain radiotherapy on the cognitive function and prospective memory in patients with brain metastases.【Methods】 A total of 100 patients underwent whole brain radiotherapy for brain metastases(WBRT) admitted in the hospital from April 2010 to May 2015 were selected. In 1 week before the whole brain radiotherapy, 1 week and 1~6 months after radiotherapy, the overall cognitive function included mini-mental state Examination (MMSE), Vocabulary fluency test (VFT), digit span test (DS) and the prospective memory including event-based prospective memory (EBPM) and event-based prospective memory (TBPM ) were assessed and compared.【Results】 One week after the radiotherapy, MMSE, DS, VFT and EBPM were all significantly lower than those before radiotherapy , and the difference was statistically significant (t=3.033,11.294, 5.055, 3.966,P<0.05); 1 week after radiotherapy, TBPM had no significant change after radiotherapy(P>0.05); MMSE and EBPM were significantly lower than the baseline in patients without neurological symptoms before radiotherapy(P<0.05), 4 months after radiotherapy , their MMSE and EBPM went to valley, and then gradually picked up, Patients with neurological symptoms before radiotherapy (MMSE≤ 26), MMSE and EBPM were significantly higher than the baseline level after radiotherapy (P<0.05), 4 months after radiotherapy, they went to the peak, then gradually decreased.【Conclusion】Whole brain radiotherapy has a certain effect on the overall cognitive function and prospective memory in patients with brain metastases, especially on those event-based prospective memory . The cognitive impairment was more significant in 4 months after radiotherapy. Patients of metastatic brain cancer with neurological system symptoms before radiotherapy may meet single separation phenomenon in the change of cognitive function after radiotherapy. 2016 Vol. 33 (10): 1888-1891 [Abstract] ( 73 ) HTML (1 KB)  PDF (1257 KB)  ( 92 ) 1892 Correlation between HPV16/18 Protein Expression and Clinicopathologic Features and Prognosis in Patients of Uygur and Han nationalities in Xinjiang with Head and Neck Squamous Cell Carcinoma ZHU Cheng-bin, Turdi. Guli Rarl, ZHANG Heng,et al DOI: 10.3969/j.issn.1671-7171.2016.10.007 【Objective】To investigate the correlation between HPV16/18 protein expression and clinicopathological features and prognosis in patients of Uygur and Han nationalities in Xinjiang with head and neck squamous cell carcinoma. 【Methods】Thirty cases of Uygur patients with head neck squamous cell carcinoma and thirty cases of Han patients with head neck squamous cell carcinoma were selected in the study. The expression levels of HPV16/18 in the tissues of head and neck squamous cell carcinoma were detected and the relationship between HPV16/18 protein expression and clinicopathological features and prognosis in patients with head and neck squamous cell carcinoma was analyzed. 【Results】The positive rate of HPV16/18 protein expression in Han populations was significantly higher than that of Uygur populations in the tissues of head and neck squamous cell carcinoma (70% VS 37.7%,P<0.05). In Uygur populations, HPV16/18 expression in the tissues of head and neck squamous cell carcinoma was associated with smoking history, tumor stage, pathological grading and lymph node metastasis (LNM) (P<0.05). In Han populations, HPV16/18 protein expression in the tissue of head and neck squamous cell carcinoma cancer was associated with smoking history, drinking history, tumor stage and LNM (P<0.05). The prognosis of patients with head and neck squamous cell carcinoma in Uygur populations was related to tumor stage, LNM and the expression of HPV16/18 protein (P<0.05). The prognosis of patients with head and neck squamous cell carcinoma in Han populations was related to smoking history, LNM and the expression of HPV16/18 protein (P<0.05). 【Conclusion】The expression of HPV16/18 protein is related to the clinicopathological characteristics and prognosis of patients with head and neck squamous cell carcinoma in Uygur and Han nationalities in Xinjiang, which can be used as an important index for clinical treatment and prognosis evaluation for patients. 2016 Vol. 33 (10): 1892-1895 [Abstract] ( 73 ) HTML (1 KB)  PDF (2352 KB)  ( 38 ) 1896 Prophylactic Effect of Methylprednisolone Combined with Tropisetron on Patients at High Risk for Postoperative Nausea and Vomiting after Thyroid Surgery LI Yun, ZHAO Nan, FANG Bo DOI: 10.3969/j.issn.1671-7171.2016.10.008 【Objective】To explore the prophylactic effect of methylprednisolone combined with tropisetron on patients at high risk for postoperative nausea and vomiting (PONV) after thyroid surgery. 【Method】 A total 120 patients at high risk for postoperative nausea and vomiting scheduled for radical operation of thyroid carcinoma were randomly divided into four groups with 30 cases in each. At 15 min before end of the operations, patients in the four groups of the patients were intravenously injected methylprednisolone 40 mg plus tropisetron 5 mg (group MT), methylprednisolone 40 mg (group M), tropisetron 5 mg (group T), and normal saline (group C). Incidence and severity of nausea and vomiting were recorded within 48 hours after surgery, and the visual analogue scale (VAS) score was used to calculate the severity of PONV.【Results】There were no significant differences in age, body weight, or operation time amongst the four groups (P＞0.05). Compared to groups C and T, the incidences of PONV during T0~2 h and T2 h~6 h and VAS score at T2 h and T6 h were significantly lower (P＜0.05). Compared to group C, the incidence of headache in group MT was significantly lower (P＜0.05), but the incidences of dizziness, anxiety, and hyperglycemia showed no obvious changes (P＞0.05).【Conclusion】Methylprednisolone combined with tropisetron has significant preventative effect on patients at high risk for PONV and the adverse reactions do not increase. 2016 Vol. 33 (10): 1896-1898 [Abstract] ( 88 ) HTML (1 KB)  PDF (1235 KB)  ( 64 ) 1899 Serum Tartrate-Resistant Acid Phosphatase 5b (TRACP 5b) and Carboxyterminal Cross-Linked Telopeptide of Type I Collagen (ICTP) in the Diagnosis of Bone Metastasis of Breast Cancer PEI Lei, TANG Zhang-hua DOI: 10.3969/j.issn.1671-7171.2016.10.009 【Objective】To study the clinical significance of serum tartrate-resistant acid phosphatase 5b (TRACP 5b) and carboxyterminal cross-linked telopeptide of type I collagen (ICTP) in diagnosing bone metastasis of breast cancer. 【Methods】A total of 714 patients in the Breast and Thyroid Department of the Second Xiangya Hospital were included in this study and were divided into two groups based on the occurrence of bone metastasis. The bone metastasis group had 74 patients and the non-bone metastasis group had 640 patients. Serum ICTP and TRACP 5b were measured by using the ELISA method. The cut-off values were calculated using the receiver operating characteristic curve (ROC) method, and the sensitivity and specificity of each serological diagnostic method were compared. 【Results】The levels of serum ICTP and TRACP 5b were higher in the bone metastasis group than in the non-bone metastasis group with statistical significance (P<0.01). The cut-off values of serum ICTP and TRACP 5b were 5.2μg/L and 5.6 U/L, respectively. Sensitivity was 81.1% and 74.3%, and specificity was 89.4% and 90.8%, respectively. The sensitivity and specificity of the combined diagnosis was 93.2% and 88.0%, respectively, and was higher than the diagnosis using either solely ICTP or TRACP 5b. 【Conclusion】Serum ICTP and TRACP 5b are important biomarkers for the diagnosis of bone metastasis in breast cancer. Combination of the two biomarkers provides very meaningful for the diagnosis. 2016 Vol. 33 (10): 1899-1902 [Abstract] ( 76 ) HTML (1 KB)  PDF (1505 KB)  ( 63 ) 1902 Correlation Analysis of Thyroid Autoantibodies and Thyroid Function in Pregnant Women WEI Yue-xiao, HE Xue-juan, WANG Ji-yun DOI: 10.3969/j.issn.1671-7171.2016.10.010 【Objective】To discuss the positive rate of serum TPOAb and TGAb in pregnant women in Cangzhou area and its relationship with thyroid-stimulating hormone (TSH) level. 【Methods】A total of 786 cases of pregnant women were divided into T1, T2 and T3 groups according to gestational week. The serum levels of TSH, TPOAb and TGAb were determined by electrochemical immunoassay. According to whether TSH increased or not, they were divided into the TSH abnormal group and TSH normal group. Based on TPOAb and TGAb levels, they were divided into the TPOAb and TGAb double positive group, TPOAb positive group, TGAb positive group, and TPOAb and TGAb double negative group. 【Results】 In the T1, T2, T3 groups of pregnancy women, TPOAb positive rates were 9.95%, 11.07% and 11.22%, respectively, while TGAb positive rates were 8.14%, 9.09% and 8.65%, respectively. The positive rates of TPOAb and TGAb in the abnormal TSH group were higher than those in the TSH normal group, and the difference was statistically significant (P<0.05). The level of TSH in the TGAb and TPOAb double positive group was increased compared with that in the TPOAb positive group, TGAb positive group, and TPOAb and TGAb double negative group; the difference was statistically significant (P<0.05). 【Conclusion】The positive rates of TPOAb and TGAb were higher in the TSH abnormal group. The level of TSH in TGAb and TPOAb double positive group was increased. TPOAb, TGAb and TSH levels all can be used as indicators for assessing thyroid function in pregnant women. 2016 Vol. 33 (10): 1902-1904 [Abstract] ( 79 ) HTML (1 KB)  PDF (1233 KB)  ( 33 ) 1905 Diagnostic Value of Tissue Doppler Imaging for Left Ventricular Diastolic Function LI De-yi, YANG Shi-li, TAO mao,et al DOI: 10.3969/j.issn.1671-7171.2016.10.011 【Objective】To explore the value of tissue Doppler imaging(TDI)in diagnosing left ventricular diastolic function. 【Methods】A total of 100 hypertensive patients in our hospital from March 2015 to June 2015 were selected as the study object. The early diastolic velocity (Ea), late diastolic velocity (Aa) and Ea / Aa of hypertension patients were detected by TDI. The early diastolic flow velocity (E), late diastolic velocity (A) and E/A were detected by color Doppler flow imaging (CDFI). In addition, 50 healthy subjects were selected as the control group. 【Results】CDFI results showed that the differences of E/A (0.59±0.13) in abnormal relaxation type patients (E/A<1) or E/A (2.24±0.17) in limited abnormal diastolic function declined type patients (E/A≥2)were statistically significant (P<0.05) from E/A (1.23±0.11) in the control group . The difference of each index between pseudo-normalization (E/A1~2) patients (91.20±7.12cm/s、69.17±6.06 cm/s、1.27±0.08) and the control group patients (85.90±9.21 cm/s、67.78±8.77 cm/s、1.23±0.11) was not significant (P>0.05). TDI results showed that the differences in Ea/Aa of abnormal relaxation in diastolic function weakened type patients (0.59±0.06) and pseudo-normalization patients (0.55±0.04) had statistically significant (P<0.05) from the control group patients (1.38±0.07).The differences in Ea and Aa of limited abnormal diastolic function declined type patients (4.01±0.31 cm/s、3.17±0.24 cm/s, respectively) and control group patients (15.12±0.87 cm/s、10.12±0.53 cm/s, respectively) were statistically significant (P<0.05). 【Conclusion】Compared to color Doppler flow imaging, TDI is more sensitive to the mitral annulus movement spectrum in patients with hypertension. It can more accurately reflect the changes of mitral annulus diastolic velocity, which is valuable in the evaluation of left ventricular diastolic function. 2016 Vol. 33 (10): 1905-1908 [Abstract] ( 82 ) HTML (1 KB)  PDF (1766 KB)  ( 64 ) 1908 Clinical and Pathological Analysis of P16 and Ki-67 in Cervical Intraepithelial Neoplasia WANG Li-hong, WANG Wen DOI: 10.3969/j.issn.1671-7171.2016.10.012 【Objective】To investigate the clinical significance of Ki-67 and P16 protein expression in the diagnosis of cervical intraepithelial neoplasia. 【Methods】Ninety-six patients with cervical intraepithelial neoplasia (CIN) were selected from our hospital from January 2013 to January 2016. Cervical biopsy specimens of all the patients were collected. According to the results of cervical biopsy, the patients were divided into a CIN Ⅰ group with22 cases, a CIN Ⅱ group with 26 cases, a CIN group with 28 cases and 20 cases of benign lesions. The expressions of P16 and Ki-67 protein in each group were analyzed and compared. 【Results】The difference in positive rates of P16 and Ki-67 were statistically significant (P<0.05); in the CIN Ⅱ and CIN Ⅲ groups the positive rates of P16 and Ki-67 were higher than that of CIN Ⅰ group (P<0.05), while the positive rate of the benign group was significantly lower than those of the other three groups (P<0.05). The positive rates of the CIN Ⅱ and Ⅲ groups were higher than that of the CIN Ⅰ group (P<0.05); the positive rate of CIN Ⅲ group was higher than that of CIN Ⅱ group (P<0.05). No positive expression was detected in the benign lesion group. The positive expressions of high-risk HPV-DNA in CIN Ⅰ, Ⅱ and Ⅲ groups were 31.82% (7/22), 65.38% (17/26) and 85.71% (24/28), respectively, and the differences among each group were statistically significant (χ2=15.464,P=0.000). 【Conclusion】The expression of Ki-67 and P16 protein is closely related to the progression of cervical intraepithelial neoplasia; the combined detection of Ki-67 and P16 can be used as an important method for the diagnosis of cervical intraepithelial neoplasia. 2016 Vol. 33 (10): 1908-1910 [Abstract] ( 114 ) HTML (1 KB)  PDF (1237 KB)  ( 54 ) 1911 Protective Effect of Adipose Derived Stem Cells on Apoptosis of Rat Pancreatic Islet Cells WANG Wen -yue, YANG Cheng-can, WANG Bing DOI: 10.3969/j.issn.1671-7171.2016.10.013 【Objective】To investigate the protective effect of adipose derived stem cells (ADSCs) on the apoptosis of rat pancreatic islet tumor cells (INS-1) induced by palm acid (PA).【Methods】The experiments were divided into three groups. Blank control group: normal medium +INS-1 to be cultured alone (the upper layer contained medium only); Group PA: high fat medium containing 0.5 mmol/L PA + INS-1 to be cultured alone; INS-1+ADSCs group: high fat medium +INS-1+ADSCs for culture. After treatment, The survival rate of the cells, the basal insulin secretion (BIS) and the glucose stimulated insulin secretion (GSIS) were detected after 24 h treatment ; The expression of Ins-1, Glut-2 and Pdx-1 in pancreatic islet cells was detected by RT-PCR; levels of IL-6, TNF-α, HGF, TIMP-1 and TGF-βwere detected by Elisa.【Results】Cell survival rate of PA group and PA+ADSCs group was lower than that in control group (P<0.01), but the survival rate of the PA+ADSCs group was significantly higher than that of the PA group (P<0.01). Compared with the control group, BIS in the PA group and the PA+ADSCs group increased (P<0.01), while the GSIS were reduced (P<0.01); Compared with the PA group, there was no significant difference in BIS cells in the PA+ADSCs group (P>0.05), Compared with the control group, the levels of Pdx-1、Ins-1、Glut-2 mRNA in the PA group and the PA+ADSCs group were significantly decreased (P<0.01); Compared with the PA group, the levels of Pdx-1 and Glut-2 mRNA in the PA+ADSCs group were significantly increased (P<0.01); while there was no significant difference in the level of Ins-1mRNA (P>0.05). The contents of IL-6, TNF-α, HGH, TIMP-1 and TGF- in the PA+ADSCs group were significantly higher than those in the other two groups.【Conclusion】PA can induce the apoptosis of INS-1 cells and inhibit the expression of Ins-1, Glut-2 and Pdx-1. ADSCs can protect the islet cells by the mechanism of side secretion and by the increase of the expression of Glut-2 and Pdx-1. 2016 Vol. 33 (10): 1911-1914 [Abstract] ( 63 ) HTML (1 KB)  PDF (1382 KB)  ( 90 ) 1915 Prenatal gene diagnosis of Thalassemia of 103 cases in Chenzhou District TAN Ju-fang, LI Cai-yun, LEI Dong-zhu DOI: 10.3969/j.issn.1671-7171.2016.10.014 【Objective】 To study on the prenatal Thalassemia gene analysis for pregnant women in order to avoid and reduce birth defects by intermediate and severe Thalassemia. 【Methods】 One hundred and three high-risk couples diagnosed as the same Thalassemia in our hospital from January 2013 to April 2015 were selected. According to the different pregnancy, amniotic fluid or umbilical cord blood was extracted to analyze fetal Thalassemia gene. Gap-PCR was used to detect the 3 common deletional α- Thalassemia, and 17 common β-Thalassemia and 3 non deletional α- Thalassemia were detected by PCR-RDB.【Results】 Among 103 couples with the same Thalassemia, 90 cases received prenatal diagnosis, the prenatal gene diagnose rate was 87.38 %. The results showed that 12 cases(13.33 %) with severe α-thalassemia, 8 cases(8.98 %) with intermediate α-Thalassemia and 14 cases(15.56%) with severe β-Thalassemia . Among the intermediate or severe thalassemia fetuses, except for 3 HbH fetuses whose parents selected to continue pregnancy, the rest chose to terminate pregnancy under informed consent. The results of induced labor were consistent with the prenatal diagnosis. 【Conclusion】 Prenatal diagnosis on high-risk couples with the same Thalassemia can effectively block the Bart 's fetal edema and the birth of children with severe Thalassemia. 2016 Vol. 33 (10): 1915-1917 [Abstract] ( 101 ) HTML (1 KB)  PDF (1206 KB)  ( 70 ) 1917 Correlation between the Intact Parathyroid Hormone(iPTH)Levels of Children Primary Nephrotic Syndrome (PNS) and 25 Hydroxy Vitamin D MENG Xian-mei,DANG Xi-qiang DOI: 10.3969/j.issn.1671-7171.2016.10.015 【Objective】 To understand the correlation between the intact parathyroid hormone(iPTH)levels of children primary nephrotic syndrome (PNS) and 25 hydroxy vitamin D( 25 (OH) D) so as to investigate the status of parathyroid function in children with PNS.【Methods】One hundred and thirty-one PNS cases with complete clinical data in our hospital from June 2013 to March 2015 were enrolled as the study group,and 60 healthy check-up cases in the same period of time were enrolled as the control group. The relationship between serum iPTH levels and serum 25 (OH) D were analyzed .【Results】 (1)The 25 (OH) D of the primary group (28.31±16.67) /L nmol was lower than that of the recurrence group (38.99±21.41) /L nmol, the difference was statistically significant (P<0.05); the iPTH of the non frequent recurrence (2.48±1.50) was lower than that of the frequent recurrence (3.83±2.35) pmol/L, the difference was statistically significant (P<0.05). (2) Pearson correlation analysis showed that blood iPTH was positively correlated with 25 (OH) D (r= 0.4737, P<0.05) in the low the iPTH group of the recurrence group, and was positively correlated with the product of calcium and phosphorus, (r=0.5046, P<0.05). (3) 4 cases with PNS were followed up for 4 months to one year and a half, and the function of the parathyroid gland was recovered.【Conclusion】 (1) Children with PNS present a lower level of serum iPTH partially. (2) Serum iPTH level is correlated with serum phosphorus, the product of calcium and phosphorus and 25 (OH) D levels; (3) Decline phenomenon of PNS parathyroid function may be temporary, and is recoverable after calcium supplements and treatment of calcitriol. 2016 Vol. 33 (10): 1917-1920 [Abstract] ( 97 ) HTML (1 KB)  PDF (1287 KB)  ( 45 ) 1921 Predictive Value of Color Doppler Ultrasound (CDU) on Preeclampsia by Testing Arteriae Uterine REN Li-juan, KONG Yan-liang DOI: 10.3969/j.issn.1671-7171.2016.10.016 【Objective】To explore the predictive value of Color Doppler Ultrasound (CDU) detection of pregnant uterine and arterial hemodynamic changes in pregnant women with preeclampsia.【Methods】A total of 90 cases of women were selected for this study. All of these women had their regular prenatal exams and delivery in our hospital. The patients were divided into three groups based on their pre-delivery characteristics: the hypertension group, preeclampsia group, and control group. All pregnant women were given the color Doppler ultrasonography during 22 ~ 24 weeks of gestation. The S/D(systolic/diastolic), PI(pulse index), RI(resistance index), and V-wave of the early diastole of the uterine arteries were compared between the three groups.【Results】The S/D, PI, and RI of patients in the hypertension and preeclampsia groups were much higher than in patients of the control group (P<0.05 ). No subjects in the control group, with the normal pregnant women, was found to have the V-wave form in either side of the uterine artery. There were 18 subjects in the hypertension group who were found to have the V-wave characteristics in both sides of the uterine artery, 12 were found with the V-wave on only the left side, and 2 were found to have the V-wave only on the right side. In the preeclampsia group, 21 women had the V-wave notch in both sides, 7 only on the left, and 2 only on the right.【Conclusion】The use of color Doppler ultrasound in testing uterine artery hemodynamics of pregnant women can lead to an early prediction of preeclampsia and provide valuable evidence for its early diagnosis and treatment. 2016 Vol. 33 (10): 1921-1923 [Abstract] ( 76 ) HTML (1 KB)  PDF (1207 KB)  ( 93 ) 1923 A Comparison of the Curative Effect of Different Hemostatic Schemes in Cesarean Section Women with Placenta Previa PAN Chang-qing, WANG Dan, ZHANG Yao ,et al DOI: 10.3969/j.issn.1671-7171.2016.10.017 【Objective】To investigate the curative effects of different hemostatic schemes in cesarean section women with placenta previa. 【Methods】A retrospective analysis of 60 patients in our hospital with placenta previa with bleeding amount greater than 500 ml during the cesarean section operation were included in this study. According to whether or not they used the Bakri balloon catheter uterine tamponade hemostasis, the 60 cases were divided into the control group and the study group, with 30 cases in each group. The study group used Bakri uterine tamponade hemostasis balloon catheter, and the control group just used routine hemostatic method for hemostasis. Twenty-four hours postpartum, the bleeding volume, operation time, intraoperative blood transfusion rate, hemostatic efficiency, and hysterectomy were analyzed. 【Results】The rate of hysterectomy, blood transfusion, amount of bleeding, and the rate of blood transfusion was significantly lower in the study group than in the control group. The operation duration in the study group (45.2 min±7.5 min) was significantly shorter than in the control group (67.3 min±5.9 min). The hemostasis rate in the study group was 93.3%, which was significantly higher than that in the control group of 76.7%.【Conclusion】Bakri balloon catheter uterine tamponade hemostasis can effectively control the placenta previa cesarean section womens' intraoperative and postoperative blood loss, intraoperative bleeding, and rate of blood transfusion during the operation. It can also improve hemostatic efficiency, and reduce the maternal uterus resection rate. 2016 Vol. 33 (10): 1923-1925 [Abstract] ( 76 ) HTML (1 KB)  PDF (1233 KB)  ( 51 ) 1926 Effect of PFM with Different Materials on the Levels of Inflammatory Factors in Periodontal Tissue and Gingival Crevicular Fluid of Patients YANG Tian-tian, GONG Zheng-lin DOI: 10.3969/j.issn.1671-7171.2016.10.018 【Objective】To investigate the effects of porcelain fused to metal crown (PFM) of different materials on the levels of inflammatory factors in periodontal tissue and gingival sulcus. 【Methods】A total of 60 patients who underwent dental restoration were selected from January 2013 to June 2015 and divided according to the repairing materials into group A (nichrome procelain crowns), group B (nickel-chrome gold-plated porcelain fused-to-metal crowns) and group C (gold-deposited porcelain crowns), with 20 cases in each group. The amount of Gingival crevicular fluid (GCF), sulcus probing depth (GCD), gingival index (GI) and tumor necrosis factor (TNF-α) alpha in gingival crevicular fluid were measured before and 6 months after repair. Interleukin -1 beta (IL-1β), interleukin -6 (IL-6) and interleukin -8 (IL-8) level were measured. 【Results】Before restoration, A group, B group and C group showed no significant difference in GCF, GCD and GI indexes (P>0.05). Six months after repair, the GCF, GCD, and GI indexes of patients in the A group were significantly increased (P<0.05); they were significantly higher than those of B group and C group (P<0.05). Before restoration, differences in the levels of TNF-α, IL-1β, IL-6 and IL-8 in gingival crevicular fluid of patients in A group, B group and C group had no statistical significance (P>0.05); 6 months after repair, TNF-α, IL-1 beta and IL-8 levels in patients in the A group were significantly increased compared to before restoration (P<0.05) and were significantly higher than those of B group and C group (P<0.05). 【Conclusion】The impact of nichrome alloy PFM on periodontal tissue in patients is more pronounced with higher levels of inflammatory factors in GCF. 2016 Vol. 33 (10): 1926-1928 [Abstract] ( 56 ) HTML (1 KB)  PDF (1230 KB)  ( 38 ) 1929 Study on Blood Purification Scheme in Patients with Acute Decompensated Heart Failure Secondary to Type 1 Cardiorenal Syndrome CHEN Dong-ying, LI Zhi-liang DOI: 10.3969/j.issn.1671-7171.2016.10.019 【Objective】 To investigate the blood purification scheme in patients with acute decompensated heart failure(ADHF) secondary to Type 1 cardiorenal syndrome (CRS).【Methods】 One hundred and ninety-six patients with acute decompensated heart failure(ADHF) secondary to Type 1 cardiorenal syndrome (CRS) admitted by the Department of Cardiology and the Department of Renal Medicine in our hospital were selected from February 2012 to February 2015. According to different blood purification scheme they were divided into 4 groups: group A (n=40, pure ultrafiltration solution), group B (n=42, slow continuous ultrafiltration), group C (n=52, intermittent hemodialysis) and group D(n=62, continuous venovenous hemofiltration, CVVH). Serum urea (Urea), creatinine (SCr), B type natriuretic peptide (BNP), cardiac troponin I (cTn-I), left ventricular end diastolic diameter (LDV), cardiac output (CO), left ventricular ejection fraction (LVEF) and central venous pressure (CVP) of patients were detected before and after treatment .【Results】Before treatment, the difference of comparison in serum Urea, SCr, BNP, cTn-I of the four groups of patients was not statistically significant (mean P>0.05); After treatment, levels of the serum Urea, SCr, BNP and cTn-I in the four groups were lower than those before treatment (except for the cTn-I level in the Group A), and the difference was statistically significant (mean P<0.05); After treatment, the differences between the groups were statistically significant (mean P<0.05). In comparison of LDV, CO, LVEF, CVP of the four groups of patients before treatment , the difference was not statistically significant (mean P>0.05); After treatment, CVP, LDV of the four groups of patients were lower than those before treatment, and LVEF, CO were higher than those before treatment, and the differences between the groups were statistically significant (mean P<0.05).【Conclusion】The adoption of continuous venovenous hemofiltration (CVVH) for blood purification in patients with acute decompensated heart failure(ADHF) secondary to Type 1 cardiorenal syndrome (CRS) is of high security with good curative effect ; Slow continuous ultrafiltration and intermittent hemodialysis have similar effects in terms of safety and efficacy; And the safety and efficacy of ultrafiltration alone is not satisfactory. 2016 Vol. 33 (10): 1929-1931 [Abstract] ( 66 ) HTML (1 KB)  PDF (1236 KB)  ( 48 ) 1932 A Comparative Study of Etomidate and Propofol in Anesthesia for Patients Undergoing Cardiothoracic Surgery WEN Xiao-juan DOI: 10.3969/j.issn.1671-7171.2016.10.020 【Objective】To compare the application effects of etomidate and propofol in anesthesia for patients undergoing cardiothoracic surgery. 【Methods】Ninety-two patients who underwent elective intracardiac surgery under extracorporeal circulation i.e. cardiopulmonary bypass (CPB) were randomly divided into group A and group B, with 46 cases in each. Group A was given etomidate anesthesia while group B was given propofol anesthesia. The hemodynamic indexes (SBP, DBP, HR) were measured before anesthesia (T1), 1 minute after induction (T2), 5 minutes after intubation (T3), 1 minute and 5 minutes after incision (T4 and T5), 1 minute and 5 minutes after splitting the sternum (T6 and T7), before CPB (T8), before the CPB aortic block (T9), 5 minutes after the CPB aortic block was released (T10), at anesthetic machine shutdown (T11), at the end of the surgery (T12) and 24 hours after the surgery (T13). Inflammatory factors (TNF-α, IL-6, IL-8, IL-1) and lipid peroxidation indexes (SOD, MDA) of the two groups before induction (t1), after induction (t2), 5 minutes after the aortic block was released (t3), at anesthetic machine shutdown (t4), 4 hours after anesthetic machine shutdown (t5), and 24 hours after surgery (t6) were compared. 【Results】 SBP and DBP of both groups fluctuated significantly at T2, T7, T8, T9,T10 and T11 compared to T1 of the same group (P<0.05). At T2, T7 and T8, the levels of group A were higher than those of group B (P<0.05). HR of the two groups fluctuated significantly at T4, T5, T8, T9, T11 and T12 compared to T1 of the same group (P<0.05), but there was no significant difference between the two groups (P>0.05). TNF-α of the two groups at t4 and t5, IL-6 at t3, t4 and t5, IL-8 at t4, IL-10 at t3 and t4 were higher compared to t1 of the same group (P<0.05), while TNF-α of group A at t4 was higher than that of group B and IL-10 at t3 and t4 were lower than those of group B (P<0.05). In the two groups, SOD at t4 and MDA at t5 were higher than those at t1 (P<0.05), while MDA in group A at t5 was higher than that in group B (P<0.05). 【Conclusion】 Etomidate is more conducive to the maintenance of the stability of hemodynamics in patients undergoing CPB open heart surgery, while propofol can play a role in myocardial protection by reducing inflammation and lipid peroxidation, which is suitable for patients with cardiac function impairment. 2016 Vol. 33 (10): 1932-1935 [Abstract] ( 61 ) HTML (1 KB)  PDF (1236 KB)  ( 68 ) 1935 Changes of Serum Lipids in Schizophrenic Patients after Withdrawal of Risperidone Treatment CAI Li-li, LI Li-qun, LI Yang,et al DOI: 10.3969/j.issn.1671-7171.2016.10.021 【Objective】To investigate the changes of serum lipids in schizophrenic patients after withdrawal of risperidone treatment.【Methods】 Ninety-three cases of schizophrenia in our hospital were divided into 3 groups, 33 patients (group A) were not treated with antipsychotic drugs, 31 patients (group B) were treated with antipsychotic drugs, and 30 patients ceased antipsychotic treatment the risperidone for at least 3 months or more (group C). In addition, 32 healthy volunteers were selected as normal control group (group D).【Results】 The general clinical data and demographic data of all the patients were collected, and levels of body weight, waist and hip circumference, fasting serum three triglycerides (TG), total cholesterol (TC), high density lipoprotein (HDL-C), low density lipoprotein cholesterol (LDL-C), apolipoprotein A1 (apoA1) and apolipoprotein A2 (apoA2) were measured. BMI of the Group C was significantly higher than that of the Group A, the difference was statistically significant (P<0.05); The serum HDL level of the Group B and the Group C was significantly lower than that of A group, and the difference was statistically significant (P<0.05).【Conclusion】 The withdrawal of risperidone treatment in schizophrenic patients can not reverse the abnormal serum lipids in the short term. 2016 Vol. 33 (10): 1935-1937 [Abstract] ( 108 ) HTML (1 KB)  PDF (1232 KB)  ( 91 ) 1938 Application of Patient-controlled Hydromorphone Analgesia in Elderly Patients with Esophageal Cancer after Thoracotomy GAO Min, LIU Wen-xiong, NING Jun-ping DOI: 10.3969/j.issn.1671-7171.2016.10.022 【Objective】To explore the application value of patient-controlled hydromorphone analgesia in elderly patients with esophageal cancer after thoracotomy. 【Methods】Eighty-two cases of elderly patients with esophageal cancer who underwent elective thoracotomy were studied. According to the random number table method, the patients were divided into the control group and the observation group, with 41 cases in each. The control group was given routine patient-controlled intravenous analgesia, while the observation group was given patient-controlled epidural hydromorphone analgesia. The changes of T lymphocytes (Th1 and Th2) in the two groups were measured. Changes of Cor, GSH-Px, Cu-Zn SOD and other stress indexes in the two groups before and after operation were recorded. The pain and sedation degree in the two groups were evaluated by VAS and Ramsay scale 4h, 8h, 12h, 24h and 48h after operation. The incidence rates of adverse reactions in the two groups were recorded. 【Results】 ① After operation, Th1 in the two groups decreased, while Th2 increased. Compared to those before operation, the differences were statistically significant (P<0.05). The decreasing range of Th1 in observation group was lower than that in the control group, and the increasing range of Th2 was also lower than that in the control group (P<0.05). ②After operation, Cor in the two groups were increased, while GSH-PX and Cu-Zn SOD were decreased. Compared to those before operation, the differences were statistically significant (P<0.05). The varied ranges of indexes in the observation group were lower than those in the control group (P<0.05). ③ Compared to postoperative 4h, VAS scores of the two groups were decreased (P<0.05). The VAS scores in different periods were lower than those of the control group (P<0.05). ④Compared to postoperative 4h, the Ramsay scores of the two groups were decreased (P<0.05). The Ramsay scores of the observation group in postoperative 4h and 8h were lower than those of the control group (P<0.05). There was no significant difference between the two groups in the scores at other time periods (P>0.05). ⑤ The incidence rates of different adverse reactions in observation group were lower than those in the control group. However, only the difference in the incidence of respiratory inhibition was statistically significant (P<0.05). 【Conclusion】The overall analgesic effect of patient-controlled hydromorphone analgesia in elderly patients with esophageal cancer after thoracotomy is good. It can relieve pain degree of patients and the sedative effect is also good. It can narrow stress factors, and has little effect on immune regulation, with high safety. 2016 Vol. 33 (10): 1938-1940 [Abstract] ( 80 ) HTML (1 KB)  PDF (1234 KB)  ( 70 ) 1941 Clinical Effect of Oxycodone Hydrochloride Injection on Postoperative Analgesia after Esophageal Carcinoma Operation XU Tao, LI Guang-shun DOI: 10.3969/j.issn.1671-7171.2016.10.023 【Objective】To observe the effect of oxycodone hydrochloride injection on postoperative analgesia after esophageal carcinoma operation. 【Methods】A total of 80 patients at our hospital for surgery as treatment of esophageal cancer surgery were divided according to postoperative analgesic drugs used into a control group of 40 patients and an observation group of 40 cases. The control group received morphine sulfate + tropisetron saline mixed analgesic, while the observation group received oxycodone hydrochloride injection in addition to the tropisetron + saline mixed analgesic. The postoperative analgesia and incidence of adverse reactions were compared between the two groups. 【Results】 The two groups showed no difference in blood pressure and heart rate level 5 min post-operation, but after 30 min, blood pressure was lower in the observation group than in the control group, while heart rate level was higher (P<0.05). VAS (Visual Analog Score) of patients in the observation group 2h, 4h, 12h was significantly lower than that of the control group (P<0.05). The recovery time of patients in the observation group was significantly shorter than that of patients in the control group (P<0.05). The rate of occurrence of postoperative adverse events in the observation group was 7.5%, significantly lower than the rate of 22.5% in the control group (P<0.05). 【Conclusion】Postoperative esophageal carcinoma treated with oxycodone hydrochloride injection + tropisetron over saline mixed had good analgesic effect and results in a low incidence of adverse reactions. It should be widely applied. 2016 Vol. 33 (10): 1941-1943 [Abstract] ( 109 ) HTML (1 KB)  PDF (1209 KB)  ( 79 ) 1943 SOX Neoadjuvant Chemotherapy on the Surgical Removal and Postoperative Complications of Gastric Cancer Surgery in Advanced Stage Patients SONG Hua-yong, ZHANG Fan, MA Lei DOI: 10.3969/j.issn.1671-7171.2016.10.024 【Objective】To explore the effects of the SOX neoadjuvant chemotherapy on the surgical clearance and complications in patients with advanced gastric cancer stage Ⅱ~Ⅲ and undergoing gastric cancer surgery. 【Methods】One hundred and fifty cases of advanced gastric cancer were admitted into the study. The study subjects were randomly divided into the observation group (54 cases) and control group (51 cases). The control group was only given the tradition gastric cancer surgery. The observation group underwent SOX neoadjuvant chemotherapy before undergoing traditional gastric cancer surgery. After the procedure, the postoperative clearance and complications were compared between the two groups. 【Results】The observation group, through TNM staging, showed that tumor stage was significantly lower (P<0.05). The observation group's surgical removal rate (90.74%) was significantly higher than that of the control group (P<0.05). The operative time (167.92 min ± 46.62 min) and intraoperative blood loss (239.45 mL±32.96 mL) were significantly higher than in the control group (P<0.05). The postoperative complication rate was not significantly different between the two groups (P>0.05). 【Conclusion】Application of SOX neoadjuvant chemotherapy in advanced gastric cancer patients undergoing gastric cancer surgery reduces the tumor stage, increases the surgical removal, and does not increase the risk of postoperative complications. 2016 Vol. 33 (10): 1943-1945 [Abstract] ( 98 ) HTML (1 KB)  PDF (1231 KB)  ( 76 ) 1946 Curative Effect of Minimally Invasive Surgery in Treatment of Hypertensive Intracerebral Hemorrhage and Study on the Influencing Factors of Rebleeding HU Xue-an, HU Shi-jie, LV Chao,et al DOI: 10.3969/j.issn.1671-7171.2016.10.025 【Objective】To study the therapeutic effect of stereotactic combined with minimally invasive surgical treatment of hypertensive cerebral hemorrhage, and the risk factors of postoperative hemorrhage of hypertensive intracerebral hemorrhage. 【Methods】Retrospective analysis of clinical data of 80 cases of hypertensive cerebral hemorrhage admitted by the Department of Neurosurgery in our hospital from January 2014 to January 2016 was conducted. According to the different operation method, they were divided into observation group and control group, 40 cases in each group. The observation group was treated by stereotactic combined with minimally invasive surgery, the control group was treated with conventional big bone flap craniotomy, and the curative effect was compared between the two groups. Multivariate Logistic regression analysis was used to analyze the risk factors of postoperative rebleeding.【Results】 Operation time, hematoma absorption time, ambulation time and hospitalization time with the observation group were shorter than those of the control group, while the amount of intraoperative bleeding, hematoma size were less than the control group, the differences were statistically significant (mean P<0.05). The score of neurological deficits in the observation group was (7.5±2.5), which was significantly lower than that of the control group (11.6±2.6), and the difference was statistically significant (t=4.05,P<0.05). Incidence of postoperative rebleeding, upper gastrointestinal bleeding and central high fever in the observation group was 2.5% (1/40), significantly lower than that of the control group 22.5% (9/40), the difference was statistically significant (χ2=5.36,P<0.05). The combined diabetes mellitus, blood coagulation dysfunction, intraventricular hematoma, hematoma >40.0 mL , basal ganglia sited bleeding and irregular shape hematoma were the risk factors of the recurrence of hypertensive intracerebral hemorrhage, and all of them have statistical difference (mean P<0.05, OR>1). Among them, blood coagulation dysfunction, basal ganglia sited bleeding and irregular shape hematoma were high risk factors (>2 OR). Whether coronary heart disease was complicated with and stereotactic combined minimally invasive surgery consisted of the protective factors of rebleeding (P<0.05, OR<1).【Conclusion】 Compared with the traditional therapy, stereotactic combined with minimally invasive surgical treatment for hypertensive intracerebral hemorrhage has the advantages of shorter operative time, less bleeding, earlier bed-off , shorter hospital stay, fewer complications and less nervous dysfunction. In order to reduce the risk factors of rebleeding, particular attention should be paid to the high risk factors, such as the abnormal coagulation function, the bleeding site and the shape of hematoma. 2016 Vol. 33 (10): 1946-1949 [Abstract] ( 77 ) HTML (1 KB)  PDF (1230 KB)  ( 171 ) 1949 Clinical Effects of Laparoscopic Surgery on the Removal of Tumors on Tail of Pancreas LIU Yong-cun,GUO Cheng, ZHOU Hui DOI: 10.3969/j.issn.1671-7171.2016.10.026 【Objective】To explore the clinical effects of laparoscopic surgery on the removal of tumors on the tail of the pancreas. 【Methods】Fifty cases of pancreatic cancer patients who underwent pancreatic tail resection in our hospital were retrospectively analyzed. Of the 50 total patients, 27 had laparoscopic surgery (laparoscopic group), and 23 patients had laparotomy (abdomen-open group). The perioperative and postoperative complications were compared between the two groups of patients. 【Results】The operative time and transfusion rates were not significantly different between the two groups (P>0.05). The blood loss, incision length, postoperative analgesia rate, postoperative exhaust time, eating time, and hospital stay were significantly lower in the laparoscopic patients than in the laparotomy patients (P<0.05). Complication rates in laparoscopic patients (18.52%) were significantly lower than in the laparotomy patients (47.83%) (χ2= 4.903,P<0.05). 【Conclusion】Laparoscopic resection of cancer tumors on the pancreatic tail results in faster recovery time and fewer postoperative complications. 2016 Vol. 33 (10): 1949-1951 [Abstract] ( 93 ) HTML (1 KB)  PDF (1225 KB)  ( 97 ) 1952 Application Value of Fibroscan Combined with APRI in the Diagnosis of Cirrhosis Portal Hypertensive Gastropathy ZHAO Zhi, ZHANG Bao-hua, LIU Xiao-li DOI: 10.3969/j.issn.1671-7171.2016.10.027 【Objective】To study the relationship between Fibroscan, aspartate aminotransferase to platelet ratio index (APRI), and cirrhosis portal hypertensive gastropathy (PHG) and analyze the clinical significance of combined detection of the two methods in the diagnosis of PHG. 【Methods】A total of 116 cases of hepatitis B infection-related liver cirrhosis were detected for liver stiffness measurement (LSM), APRI, degree of esophageal varices (EV), portal vein diameter (PVD), platelet count/spleen diameter (PC/SD), and PHG. The relationships between LSM, APRI and EV, PVD, and PC/SD were analyzed and the differences of LSM and APRI were compared in varying degrees of PHG patients. Then, the receiver operating characteristic curves (ROC) of LSM and APRI were drawn to diagnose PHG. 【Results】The differences were statistically significant for LSM and APRI in varying degrees of EV. LSM and APRI have correlation with PVD and PC/SD. Also, LSM and APRI in patients with varying degrees of PHG were statistically significant. AUROC of Fibroscan for PHG was 0.80(0.71~0.89, the sensitivity was 78.91%, and the specificity was 73.08%. The AUROC of APRI for PHG was 0.80(0.70~0.90), the sensitivity was 65.83%,and the specificity was 92.27%. The AUROC of the combined method for PHG was 0.95(0.89~1.00), the sensitivity was 86.77%,and the specificity was 98.10%. 【Conclusion】FibroScan and APRI reflected the degree of portal hypertension and PHG in liver cirrhosis patients. The combined detection of the two methods was more helpful to improve the accuracy of diagnosis, and it had certain clinical value for the noninvasive diagnosis of PHG. 2016 Vol. 33 (10): 1952-1955 [Abstract] ( 109 ) HTML (1 KB)  PDF (1333 KB)  ( 68 ) 1955 Risk Factors of Nosocomial Infection in Patients after Hysteroscopy Operation YE Feng, WU Jin, YU Yan-zi DOI: 10.3969/j.issn.1671-7171.2016.10.028 【Objective】To analyze the risk factors of nosocomial infection in patients after hysteroscopy operation.【Methods】Retrospective analysis of 3150 cases of nosocomial infection after surgery in our hospital from January 2011 to June 2015 was conducted, Risk factors associated with nosocomial infection in patients was analyzed by univariate factor screening, Multivariate logistic regression analysis was used to determine the independent risk factors of nosocomial infection in patients after hysteroscopy surgery.【Results】A total of 32 cases with nosocomial infection were observed, yielding a postoperative infection rate of 1.02%(32/3150), the main infection types were myositis uterus, pelvic inflammatory disease, which accounting for 37.50% and 28.12%. The main pathogens were Gram-positive and Gram-negative bacteria, fungi each accounting for 60.98%, 31.71%, 7.32%. Univariate analysis showed that preoperative inflammation, operative time, hospital stay, surgical grade, complicated chronic disease, prophylactic use of antimicrobial agents were closely associated with postoperative nosocomial infection (P<0.05). Furthermore, multivariate logistic regression analysis showed that preoperative inflammation, prolonged operative time, higher surgical grade (Ⅳ grade), complicated chronic disease were independent risk factors associated with nosocomial infection in patients (P<0.05).【Conclusion】Factors like preoperative complicated inflammation, prolonged operative time, higher surgical grade and complicated chronic disease can increase the risk of nosocomial infection in patients with hysteroscopy. Thus, it needs to take the appropriate measures to reduce postoperative infection rates. 2016 Vol. 33 (10): 1955-1957 [Abstract] ( 78 ) HTML (1 KB)  PDF (1236 KB)  ( 104 ) 1958 Clinical Efficacy of Dexmedetomidine and Sufentanil Assisted as Topical Anesthesia in Relieving Cardiovascular Response in Endotracheal Intubation ZHAO Qi-bing, HUANG Zhao-sheng, HUANG Tai-lin DOI: 10.3969/j.issn.1671-7171.2016.10.029 【Objective】To compare the clinical efficacy of dexmedetomidine and sufentanil assisted as topical anesthesia in relieving cardiovascular response in endotracheal intubation.【Methods】 Ninety-two awake tracheal intubation patients admitted in our hospital were randomly (single blind) divided into observation group (47 cases) and control group(45 cases).Under supplementary topical anesthesia of Lidocaine and Dlcalne, the observation group was infused with dexmedetomidine before intubation, and the control group conducted intravenous injection of sufentanil. The changes of the following of the two groups were recorded and compared: Surface anesthesia (T0), immediately after intubation (T1), 3 min after intubation (T2), 5 min after intubation (T3), norepinephrine (NE), systolic blood pressure (SBP), diastolic blood pressure (DBP) and heart rate (HR).【Results】 (1)After intubation, NE, SBP and HR in the two groups showed a transient increase compared with the basal values, all of the indexes were basically restored to the level of basic values at T3;(2) At T1, NE, SBP, DBP, HR index of the control group were higher than those of the observation group, and the differences were significant (P<0.05); The difference of NE and SBP in the two groups at T2 was significant (P<0.05); there was significant difference between the two groups in NE at T3 (P<0.05).【Conclusion】Compared with Sufentanil, Dexmedetomidine used in endotracheal intubation assisted with topical anesthesia has comparative advantages to relieve cardiovascular responses. 2016 Vol. 33 (10): 1958-1960 [Abstract] ( 49 ) HTML (1 KB)  PDF (1208 KB)  ( 74 ) 1960 Analysis of Protective Effect of Trimetazidine on Renal Function in Hypertensive Patients with Coronary Heart Disease ZHANG Yi-ping DOI: 10.3969/j.issn.1671-7171.2016.10.030 【Objective】 To investigate the protective effect of Trimetazidine on renal function in hypertensive patients with coronary heart disease. 【Methods】Eighty-six cases of hypertension with coronary heart disease were randomly divided into two groups, each of 43 cases. The control group was treated with routine therapy; while on the basis the control group, the observation group was treated with Trimetazidine. One course of treatment for 4weeks, 3 courses were intervened. Blood pressure, heart function and renal function and adverse reaction conditions were compared between the two groups before and after treatment.【Results】After treatment, DBP, SBP levels, LVEDD, LVESD, SCr one week after treatment, BUN, Cys C, hs-CRP and IL-6 levels in the two groups were significantly decreased than those before treatment while LVEF was significantly increased than that before treatment (P<0.05). 3 courses after the treatment, LVEDD, LVESD, LVEF, SCr one week after treatment, BUN, Cys C, hs-CRP, IL-6 and NO levels in the observation group were significantly improved than those in the control group (P<0.05). There were no significant differences in the incidence of adverse reactions between the two groups (P>0.05). 【Conclusion】 Trimetazidine can significantly improve the cardiac function in hypertensive patients with coronary heart disease. It has also protective effect on renal function. This may be related to the drug functions of inflammation reduction and vascular endothelium improvement. 2016 Vol. 33 (10): 1960-1962 [Abstract] ( 92 ) HTML (1 KB)  PDF (1230 KB)  ( 47 ) 1963 Investigation of Sentinel Lymph Node Biopsy by Radioisotope, Blue Dye and Fluorescence Methods for Breast Cancer ZHANG Kai-tong, GUAN Shan, WANG Yu DOI: 10.3969/j.issn.1671-7171.2016.10.031 【Objective】The primary purpose of this study was to provide practical recommendations through determining the detection rate of three methods of sentinel lymph node (SLNB) biopsy: ICG fluorescence imaging, radioisotope(99mTc) and blue dye.【Methods】Thirty-four women with clinically negative-node breast cancer underwent SLNB using radioisotope, blue dye and ICG. Detection rates of every method and combinations of any two or three tracers were analyzed. 【Results】 A total of 106 sentinel lymph nodes (SLNs) were dissected, with average SLN detecting number of 3.12 in each patient. Using the radioisotope method, 91 SLNs were identified and 2.68 SLNs were detected in each patient, with a detection rate of 85.8%. The SLN detection rates for ICG fluorescence imaging and blue dye method were 94.3% and 43.4%, respectively, with 91 and 46 SLNs were detected, respectively and 2.94 and 1.35 SLNs per patient, respectively. The identification rate for ICG fluorescence method in combination with the blue dye method was 94.3%, while it was 100% for ICG in combination with the radioisotope method. The number of identified SLNs was not significantly different (P>0.05) between the radioisotope and ICG method, but the number of identified SLNs was significantly different (P<0.05) between the isotope method in combination with the blue dye method and the fluorescence method in combination with blue dye method. SLNs were identified in 33/34 patients (97.1%) with either ICG fluorescence method alone or ICG fluorescence method in combination with the blue dye method; the detection rate was equivalent to the 99mTc-labeled radiotracer method. The detection rate was 34/34 (100%) when the radioisotope method was combined with ICG or blue dye. 【Conclusion】Radioisotope method alone and combined with blue dye or fluorescence is preferred in detecting SLNs. Using fluorescence alone or combined with blue dye is a feasible and convenient procedure for clinical practice. 2016 Vol. 33 (10): 1963-1966 [Abstract] ( 95 ) HTML (1 KB)  PDF (1228 KB)  ( 74 ) 1966 Predictive Value of GRACE score, TIMI risk score, and BNP on Adverse Cardiovascular Events in Emergency Patients with Chest Pain LI Yang DOI: 10.3969/j.issn.1671-7171.2016.10.032 【Objective】To analyze the predictive value of GRACE (global registry of acute coronary events) score, TIMI (thrombolysis in myocardial infarction) risk score, and BNP (B-type natriuretic peptide) on adverse cardiovascular events in emergency patients with chest pain. 【Methods】Patients with acute chest pain who were treated in our hospital were observed. GRACE risk score, TIMI risk score, and BNP level were analyzed in patients with major adverse cardiovascular events (MACE). GRACE, TIMI risk score, and BNP were predictors of cardiovascular events during hospitalization. 【Results】The rate of MACE in patients of different GRACE scores and TIMI scores ranged from high to low and were sorted into a high risk group, medium risk group, and low risk group, with statistically significant differences amongst the groups (P<0.05). The BNP of patients who experienced MACE or death were significantly higher than that of patients who did not experience MACE and surviving patients (P<0.05). GRACE score, TIMI risk score, and BNP can predict the risk of cardiovascular events to a fitting degree. 【Conclusion】GRACE score, TIMI risk score, and BNP have good predictive values for adverse cardiovascular events in emergency patients with chest pain, and can be used as clinical indicators. 2016 Vol. 33 (10): 1966-1968 [Abstract] ( 88 ) HTML (1 KB)  PDF (1233 KB)  ( 61 ) 1969 Clinical efficacy of integrated treatment (Yiqiyangxue compound and Western medicine) on RITP and the changes of platelet membrane glycoprotein CD41 and CD61 JI Xiao-hong, LI Cao-ni, GAO Ye-ping,et al DOI: 10.3969/j.issn.1671-7171.2016.10.033 【Objective】 To investigate the clinical efficacy of integrated traditional Chinese and Western medicine in the treatment of refractory immune thrombocytopenia (RITP) and the level changes of platelet membrane glycoprotein CD41 and CD61 before and after treatment.【Methods】 Seventy-eight cases of RITP in our hospital from May 2010 to December 2014, were randomly divided into control group and observation group, 39 cases in each group.The control group was given prednisone tablets, and the observation group was given the combined treatment prednisone tablets + yiqiyangxue decoction. After 6 months of treatment, the clinical effects of the two groups and the changes of platelet count, platelet membrane glycoprotein CD41, CD61 and T cell subsets were compared before and after treatment. 【Results】 After treatment, the total efficiency of the observation group was significantly higher than the control group, the statistical difference was significant (χ2=20.146,P<0.05); After treatment, PLT, PCT, CD41, CD61 of the two groups were significantly higher than those before treatment (P<0.05), and the increase rate of the observation group was higher than that of the control group (P<0.05); After treatment, CD3+、CD4+、CD4+/ CD8+ of the two groups were significantly higher than those before treatment, CD8+ was significantly lower than that before treatment (P<0.05), the change rate of the observation group was greater than that of the control group (P<0.05); Compared with the control group, the side effects of glucocorticoid in the observation group were significantly improved (P<0.05).【Conclusion】 The clinical efficacy of combination of traditional Chinese and Western medicine for treatment of RITP is better than simple western medicine treatment. The integrated therapy can improve the cellular immune function of patients and reduce the side effects of glucocorticoid, and improve the level of platelet membrane glycoprotein CD41 and CD61. 2016 Vol. 33 (10): 1969-1972 [Abstract] ( 51 ) HTML (1 KB)  PDF (1231 KB)  ( 60 ) 1972 Clinical Analysis of 3D Power Doppler Ultrasound in Diagnosis of Benign and Malignant Thyroid Tumors MA Yu-xia, MA Ji-xian DOI: 10.3969/j.issn.1671-7171.2016.10.034 【Objective】To explore clinical value of three dimensional (3D) power Doppler ultrasound in the diagnosis of benign and malignant thyroid tumors. 【Methods】 A retrospective analysis of clinical data was carried out in 76 patients with thyroid tumors confirmed by operation or pathology in our hospital from May 2011 to July 2015, all patients had received three dimensional power Doppler ultrasound examination after admission, and the imaging data were complete, features of blood flow of benign and malignant thyroid tumors were observed, and diagnostic value of three dimensional power Doppler ultrasound for thyroid benign and malignant tumors were evaluated. 【Results】 ①Gray scale values (MG) of patients with benign thyroid tumors were significantly higher than patients with malignant thyroid tumors (P<0.05), the vascular index (VI), blood flow index (FI) and vascular blood flow index (VFI) were significantly lower than those with malignant tumor (P<0.05); ②there was no significant difference in the proportion of blood flow grading gradeⅡ in benign and malignant thyroid tumors (P>0.05), the proportion of grade Ⅰin patients with benign tumors was significantly higher than that of the malignant , and the proportion of grade Ⅲ was significantly lower than that of the malignant (P<0.05); ③the proportion of peripheral blood flow distribution , the vascular rule and the spatial distribution symmetry in benign thyroid tumors were significantly higher than those with the malignant (P<0.05); ④31 cases of benign thyroid tumors were detected by 3D power Doppler ultrasound, including 29 cases confirmed and 2 cases misdiagnosed; 45 cases of malignant tumors, among which 40 cases were confirmed and 5 misdiagnosed , and the overall diagnostic accordance rate was 90.79%(69/76). 【Conclusion】 The diagnosis of benign and malignant tumors of the thyroid gland with three-dimensional power Doppler ultrasound is in good agreement with the pathological diagnosis, which can clearly show the tumor blood flow distribution, vascular characteristics and internal structure. The operation is simple without wound, it is worth clinical application. 2016 Vol. 33 (10): 1972-1975 [Abstract] ( 115 ) HTML (1 KB)  PDF (1251 KB)  ( 63 ) 1976 Effects of Low Molecular Weight Heparin on the Proliferation and SDF-1 Expression of Primary Gastric Cancer Cells PAN Chang, WANG Shu-qing, QIU Ming-ke, et al DOI: 10.3969/j.issn.1671-7171.2016.10.035 【Objective】To investigate the effect of low molecular weight heparin (LMWH) on the proliferation and the SDF-1expression of primary cultured gastric carcinoma cells. 【Methods】Poorly differentiated adenocarcinoma of the stomach tissues and normal gastric mucosa tissues were made into primary cells and then divided into four groups:group A had gastric cancer cells, group B had gastric cancer cells + LMWH, group C had gastric mucosal cells, and group D had gastric mucosal cells + LMWH. The cell proliferation was detected by MTT assay, the cell cycle distribution and apoptosis was detected by V-FITC/PI assay, and the expression of SDF-1 mRNA was examined by semi-quantitative RT-PCR.【 Results】 The proliferation of gastric carcinoma cells in group B was much lower than in group A. At the same time, cell apoptosis was higher in group B than in group A. In the G0 and Gl phases,there was a higher ratio of cells but a lower ratio was observed in the S period. There was no significant difference in cell ratios in the G2 and M phases between these two groups.For groups C and D, there was no significant difference for cell proliferation, cell cycle, or apoptosis. The expression of SDF-1 mRNA was lower in group B than in group A. There was no significant difference in the expression of SDF-1 mRNA in groups C and D. 【Conclusion】LMWH can inhibit the proliferation of primary gastric carcinoma cells and also increase the cell apoptosis, changing the structure of the cell cycle and reducing the expression of SDF-1 mRNA in primary cells of gastric cancer. 2016 Vol. 33 (10): 1976-1979 [Abstract] ( 67 ) HTML (1 KB)  PDF (1232 KB)  ( 64 ) 1979 Effect of Colchicine on Early Recurrence and Inflammatory Factors after Catheter Ablation of Paroxysmal Atrial Fibrillation (AF) YANG Hua, XU Chen-hong, LI Xin DOI: 10.3969/j.issn.1671-7171.2016.10.036 【Objective】To investigate the effect of colchicine on early recurrence and inflammatory factors after catheter ablation of paroxysmal atrial fibrillation (AF).【Methods】A total of 80 patients with paroxysmal AF who had received catheter ablation were randomly divided into treatment group (n=40, colchicine group) and control group (n=40, placebo group).Three month after the ablation treatment, the early recurrence rate of paroxysmal AF were determined.Changes of the serum high sensitivity C-reactive protein (hs-CRP) and interleukin-6(IL-6 )levels were observed before and after the ablation treatment. 【Results】After ablation, the recurrence rate of the observation group was lower than that of the control group (P<0.05). The levels of hs-CRP and IL-6 in the observation group were significantly lower than those in the control group (P<0.05), and compared with preoperative, postoperative hs-CRP and IL-6 levels were decreased in the observation group (P<0.05), the level of hs-CRP and IL-6 in the control group was higher than that before operation (P<0.05). The treatment group was further divided into recurrent group and non recurrent group, the postoperative recurrence rate of hs-CRP and IL-6 in the non recurrence group was greater than that in the recurrence group (P<0.05).【Conclusion】 Colchicine can prevent early recurrence of paroxysmal AF after catheter ablation treatment. This effect seems to be associated with a significant decrease in inflammatory reaction. 2016 Vol. 33 (10): 1979-1981 [Abstract] ( 44 ) HTML (1 KB)  PDF (1236 KB)  ( 64 ) 1982 Study on the Gene Distribution and Relationship of Human Leukocyte Antigen (HLA) in Patients with Severe Skin Allergy Reaction Induced by Anti-Epileptic Drugs FANG Deng-fu, ZHANG Bo, CHEN Xiao-rong DOI: 10.3969/j.issn.1671-7171.2016.10.037 【Objective】To analyze the gene distribution of human leukocyte antigen (HLA) in patients with severe skin allergy reaction induced by anti-epileptic drugs. 【Methods】 Sixty patients with skin allergy reaction induced by anti-epileptic drugs who were treated between March 2013and March 2016 were selected as study subjects, including 35 patients with mild skin allergy reaction(mild group) and 25 patients with severe skin allergy reaction (severe group). At the same time, another 40 normal healthy persons were selected as control group. The distribution of HLA-B* 1502 in the three groups was determined by PCR-SSP method. The frequency of HLA-B* 1502, HLA-A* 1101、HLA-A* 3101 was compared between the three groups and the relationship between skin allergy reaction induced by anti-epileptic drugs and HLA genotype was analyzed. 【Results】The frequency of HLA-B* 1502 gene in the severe group (56.00%) was significantly higher than that in the mild group and the control group (11.43%, 7.50%) (P<0.05); Skin allergy reaction induced by anti-epileptic drugs was related to HLA-B * 1502, HLA-A * 1101, HLA-A * 3101 and HLA-B * 1511 (P<0.05), while mild skin allergy reaction had no significant correlation with genes mentioned above (P>0.05) (except for HLA-A * 3101). The severe skin allergy reactions in 25 cases were caused by the aromatic anti-epileptic drugs. 【Conclusion】 The main HLA genes of severe skin allergy reaction induced by anti-epileptic drugs are HLA-B * 1502 and HLA-B* 1511. Severe skin allergy reaction induced by anti-epileptic drugs is significantly related to positive HLA-B * 1502 and other genes. The detection of HLA-B *1502 and other genotypes is suggested before anti-epileptic drug therapy. If it is positive, aromatic antiepileptic drug treatment ought to be avoided. 2016 Vol. 33 (10): 1982-1985 [Abstract] ( 53 ) HTML (1 KB)  PDF (1234 KB)  ( 58 ) 1985 Study on the Correlation Between Inhibin A and Preeclampsia and Eclampsia LU Jing-xiang,SHI Jin-qiu, ZHOU Ming,et al DOI: 10.3969/j.issn.1671-7171.2016.10.038 【Objective】 To explore the tudy on the correlation between inhibin A and preeclampsia and eclampsia. 【Methods】Twenty-five cases of preeclampsia and 22 cases of severe preeclampsia treated were selected in our hospital from May 2014 to May 2015 as observation group, and normal pregnant women admitted in the hospital in the same period of time as the control group. Gene and protein levels were determined on the expression of the inhibin A in peripheral blood of the patients with preeclampsia and severe preeclampsia by fluorescence quantitative PCR, enzyme linked immunosorbent assay and Western-blotting technique,and the expression amount was detected by immunohistochemical staining technique. 【Results】Compared with the mRNA expression amount of inhibin A in the serum of the control group, the inhibin A level in the serum of pregnant women with preeclampsia and severe eclampsia were significantly elevated, there was significant difference between the observation group and the control group (P<0.05); Protein detection results showed that, compared with the serum inhibin A levels[(6.8±0.6)μg/L] of in pregnant women in the control group, the content of serum inhibin A in both preeclampsia [(12.6±1.2)μg/L] and severe preeclampsia [(38.4±1.3) μg/L] was significantly increased, and there were significant differences in the levels of inhibin A in both of them (P<0.05). By immunohistochemistry, the expression of inhibin A in the severe preeclampsia was higher and eclampsia, this was consistent with the results of RT-PCR,ELISA and Western-blotting.【Conclusion】 There is a correlation between the presence of inhibin A and the incidence of eclampsia, As inhibin A levels rise, the eclampsia illness is aggravating gradually. 2016 Vol. 33 (10): 1985-1989 [Abstract] ( 61 ) HTML (1 KB)  PDF (1734 KB)  ( 55 )

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