生长激素释放肽对乳腺癌耐药蛋白表达的影响及意义

doi:10.3969/j.issn.1671-7171.2016.10.003

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Abstract 【Objective】 To study the effects of ghrelin on the expression of breast cancer resistance protein(BCRP) in breast cancer cell line. 【Methods】Human breast cancer cell line MDA-MB-231 were cultured and treated with ghrelin, doxorubicin, and ghrelin combined with doxorubicin. The tunnel method was used to detect cell apoptosis. The Western-blot method was used to detect p-P38, P38, and BCRP protein levels. 【Results】Tunnel assay showed that under the addition of ghrelin, the human breast cancer cell MDA-MB-231 proliferated and the apoptosis rate was significantly lower than when doxorubicin was used. Ghrelin combined with doxorubicin inhibits the killing effect of doxorubicin on the human breast cancer cell line MDA-MB-231(P<0.001). The P38 and p-P38 protein levels increased in the Ghrelin Group, and the protein expression of BCRP increased significantly as well. The P38 and p-P38 protein levels and BCRP protein expression decreased in the Ghrelin combined with doxorubicin group, but the decrease was less compared to the doxorubicin group (P<0.05). 【Conclusion】The P38MAPK signaling pathway activated by ghrelin promotes breast cancer cell BCRP expression, inhibiting the killing effects of doxorubicin-induced apoptosis of breast cancer cells.

Key words： Growth Hormone-Releasing Hormone Peptides Breast Neoplasms/DT Drug Tolerance Tumor Cells, Cultured

Received: 31 December 2015

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R737.9

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http://www.jcr.net.cn/EN/10.3969/j.issn.1671-7171.2016.10.003 OR http://www.jcr.net.cn/EN/Y2016/V33/I10/1880

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