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Quick Search     Adv Search 2017 Vol. 34, No. 9 Published: 2017-09-30 Article          Article 1665 Clinical Analysis of Percutaneous Transhepatic Cholangioscopic Lithotomy (PTCSL) in the Treatment of Complicated Hepatolithiasis MA Pu-yu, JIANG Chao, CHEN Chen,et al DOI: 10.3969/j.issn.1671-7171.2017.09.001 【Objective】To analyze clinical therapeutic effects of percutaneous transhepatic cholangioscopic lithotomy (PTCSL) in the treatment of hepatolithiasis.【Methods】A total of 120 cases of patients with complicated hepatic bile duct stones treated in our hospital from February 2014 to December 2016 were selected as the research objects. Sixty cases were treated with PTCSL, while another sixty cases were treated with conventional treatment. The observation group (group PTCSL) was treated with percutaneous transhepatic cholangioscopic lithotomy (PTCSL) with Holmium laser. The routine group was treated with traditional open surgery; The calculi removal rate, intraoperative blood loss and postoperative complications of the two groups were statistically analyzed.【Results】In the group PTCSL, the calculi removal rate was 80.0%.In the routine group, the calculi removal rate was 31.67%,which was lower than that of group PTCSL; the difference between the two groups was statistically significant (P＜0.05).The mean bleeding volume of group PTCSL (18.31±10.12) mL was significantly lower than that of the routine group (317.76±41.30)mL; there was significant difference between two groups (P＜0.05). The incidence of postoperative complications in group PTCSL was 15.23%, lower than that of the routine group, which was was 40.67%; the difference was significant (P＜0.05). In group PTCSL, the postoperative hospital stay was shorter than the routine group;there was significant difference between two groups (P＜0.05).【Conclusions】Percutaneous transhepatic cholangioscopic lithotomy (PTCSL)is a safe and reliable method for the treatment of complicated hepatolithiasis. It has the advantages of high stone removal rate, less bleeding, fewer complications, shorter postoperative hospital stay, and is suitable for clinical use. 2017 Vol. 34 (9): 1665-1667 [Abstract] ( 60 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1668 Protective Effect of Endostatin on Acute Lung Injury of Septic Mice PENG Yue, GAO Min, OU Hao,et al DOI: 10.3969/j.issn.1671-7171.2017.09.002 【Objective】To investigate the protective effect of endostatin on acute lung injury in septic mice and to explore its mechanisms behind these protective effects.【Methods】One hundred and fifty male (n=150) Kunming mice were randomly divided into the following groups: sham, septic model and endostatin treatment groups. The septic model was established by cecal ligation and puncture(CLP). Mice in the treatment group were administrated endostatin (2mg/kg, ip) after the operation while those in the sham and septic model group were treated with equivalent amount of saline instead. Survival rates were observed for up to 3 days. The effects of endostatin on the expression of TNF-α, IL-6 and VEGF in the serum were assessed. In addition, we examined the effects of endostatin on Evans blue leakage, wet to dry weight ratio, histology and the phosphorylation of p38 MAPK proteins in lung tissues of septic mice.【Results】We found that endostatin increased the survival of septic mice and reduce the Evans blue leakage of lung tissues, lung wet to dry weight ratio and morphologic changes. In addition, endostatin reduced serum TNF-α, IL-6 and VEGF-C levels in septic mice and inhibit phosphorylation of p38 in lung tissues of septic mice.【Conclusions】Endostatin can inhibit inflammation and improve vasopermeability and regulate the p38 MAPK signaling pathway in lung and exert protective effect on acute lung injury of septic mice. 2017 Vol. 34 (9): 1668-1672 [Abstract] ( 50 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 1673 Effect of Erdosteine on the Expression of Nrf2 in Mice Acute Lung Injury DONG Liang, ZHOU Yi-qi,MAO Zhen-yu,et al DOI: 10.3969/j.issn.1671-7171.2017.09.003 【Objective】To investigate the effect of erdosteine on the expression of antioxidant transcription factor Nrf2 in lung of acute lung injury (ALI) mice induced by lipopolysaccharide (LPS).【Methods】The healthy male C57bl/6 mice were randomly divided into control group, ALI group and erdosteine group (n=8). The mouse model of ALI was reproduced by intratracheal injection of LPS (5 mg/kg). Erdosteine (150 mg/kg) was given 30 min before delivery and the mice were sacrificed 12 h later. HE staining was used to observe the morphological changes of lung tissue in mice. The content of malondialdehyde (MDA) and superoxide dismutase (SOD) were measured to reflect the oxidative stress in lung tissue. The expression of Nrf2 in lung tissue was detected by Western blot. Real-time PCR was used to detect the contents of HO-1 and SOD mRNA in lung tissue.【Results】Erdosteine could improve the histopathological changes of lung tissue induced by LPS in ALI mice, decrease the content of MDA in lung tissue and increase the activity of SOD. Erdosteine could also significantly increase the expression of Nrf2 in the nucleus of ALI mice and significantly increase the expression of HO-1 and SOD mRNA.【Conclusion】Erdosteine can alleviate LPS-induced ALI in mice.The mechanism may be due to the enhancement of the nuclear translocation and transcription activity of the antioxidant transcription factor Nrf2 in the lung of ALI mice. 2017 Vol. 34 (9): 1673-1676 [Abstract] ( 75 ) HTML (1 KB)  PDF  (0 KB)  ( 9 ) 1677 Expression of HMGB1/RAGE in Hepatic Ischemia-reperfusion Injury (HIRI) and the Preconditioning Effect of Octreotide in Rats SUN Hui-ping, YANG Jin-feng, ZOU Shuang-fa,et al DOI: 10.3969/j.issn.1671-7171.2017.09.004 【Objective】To investigate the expression of HMGB1/RAGE in hepatic ischemia-reperfusion injury (HIRI) and the preconditioning effect of octreotide (Oct) in rats.【Methods】Seventy-two healthy adult male SD rats were randomly divided into three groups equally (n=24): sham operation group(group S), ischemia reperfusion group (group I/R), and octreotide group(group Oct). 30 minutes before operation, the Group Oct received intraperitoneal injection of saline containing Oct 0.25 mg/kg, while the group S and the group I/R were intraperitoneally injected with the same amount of normal saline. HIRI model was established by Pringle 's maneuver method. Blood sampling was collected to detect serum alanine transaminase (ALT) and the change of high mobility group box protein 1 (HMGB1) by immunohistochemistry at liver ischemia 30 min (T1), reperfusion time was 1 h (T2), 6 h (T3) and 12 h (T4). The liver tissue was taken at 12 h after reperfusion, the expression of HMGB1 was detected by immunohistochemistry; The expression of mRNA and protein levels of HMGB1 and advanced glycation end products receptor (RAGE) were detected by real-time quantitative PCR and Western blot respectively.【Results】Serum ALT levels increased from T1 to T4 in the group I/R and the group Oct, all of them were higher than those in the group S, the I/R group was significantly higher than the Oct group, the difference was statistically significant (P＜0.05); The level of HMGB1 increased from T1 to T3, T3 peaked and T4 began to decrease, but all were higher than those in group S. The I/R group was significantly higher than the Oct group, the difference was statistically significant (P＜0.05). The HMGB1 positive cells showed the cytoplasm of nucleus and cytoplasm were brownish yellow. The positive expression of group I/R and group Oct were (6.8 ± 1.2), (4.1 ±1.5), significantly higher than that of group S (3.2 ± 1.3), and the I/R group was significantly higher than the Oct group, the difference was statistically significant (P＜0.05). Compared with group S, the expression of HMGB1, RAGE, mRNA and protein in I/R liver tissue increased significantly (P＜0.05); Compared with group I/R, the expression of HMGB1, RAGE and mRNA in Oct group decreased significantly after pretreatment with Oct (P＜0.05).【Conclusion】Oct preconditioning has obvious protective effect on HIRI in rats, which may be related to the inhibition of the expression of HMGB1 and RAGE. 2017 Vol. 34 (9): 1677-1680 [Abstract] ( 31 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 1681 Effect of Maixuekang Capsules on Quality of Life in Patients with Subacute Cerebral Infarction BIAN Ying, DU Ju-mei, YAN Jiang DOI: 10.3969/j.issn.1671-7171.2017.09.005 【Objective】T o observe the effect of Maixuekang capsules on quality of life of patients with subacute cerebral infarction.【Methods】Sixty patients with subacute cerebral infarction from January 2015 to January 2016 in our hospital were randomly divided into the control group and the observation group, 30 cases in each group. The control group received conventional treatment such as simvastatin and aspirin, the treatment group received Maixuekang capsule additionally. All were treated for 15 days.The Barthel index (BI) and the modified Rankin scale (mRS) were used to evaluate the daily living ability, prognosis and functional disability of the two groups before and after treatment. The life quality of two groups before and after treatment was evaluated by SS-QOL and SF-36 Health Survey (SF-36).【Results】Before treatment, there was no significant difference between the two groups in BI, mRS score, SS-QOL scale and SF-36 scale score (P＞0.05). After 15d treatment, BI, mRS score, SS-QOL scale and SF-36 scale score of the two groups were better than those before treatment. In the observation group, the BI, mRS score, the SS-QOL scale items like physical activities, language ability, self-care, upper limb function and the SF-36 scores like physiological function, body pain, general health, vitality were improved significantly, the difference was statistically significant (P＜0.05).【Conclusion】Maixuekang capsule can significantly improve the quality of life of patients with subacute cerebral infarction, and is worthy of clinical application. 2017 Vol. 34 (9): 1681-1683 [Abstract] ( 72 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 1684 Effect of Postoperative Chemotherapy Combined with Endocrine Therapy on the Expression of ERβ in Cancer Tissues of Advanced Prostate Cancer WANG Jin-en, CHENG Fan DOI: 10.3969/j.issn.1671-7171.2017.09.006 【Objective】To investigate the effect of postoperative chemotherapy combined with endocrine therapy on the advanced prostate cancer and the correlation of ERβ expression in cancer tissues.【Methods】A total of 120 patients with advanced prostate cancer were treated in our hospital from January 2015 to March 2017, and randomly divided into the experimental group and the control group, 60 cases in each group . The experimental group received chemotherapy and endocrine therapy, and the control group received routine chemotherapy. The curative effect difference between the two groups was observed. The correlation between the effect of treatment and the expression of ERβ in cancer tissue was analyzed according to the difference between the two groups.【Results】The effective rate of the prostate cancer patients in the experimental group was significantly higher than that of the control group, the difference was statistically significant (χ2=9.219,P＜0.05). The expression levels of ERβ in cancer tissues were lower in the effective patients of experimental group, the control group and the two groups than those of ineffective patients. The difference was also statistically significant (P＜0.05). According to the expression of ERβ in cancer tissue level and efficacy of single factor logistics regression analysis, the differences were statistically significant (P＜0.05), the maximum likelihood estimates of cancer ERβand OR＜0, which suggested that cancer ER beta expression levels were negatively correlated with the curative effect.【Conclusion】Postoperative chemotherapy combined with endocrine therapy is effective in the treatment of middle and advanced prostate cancer. The effect is negatively correlated with the expression of ER βbeta in cancer tissues. 2017 Vol. 34 (9): 1684-1686,1690 [Abstract] ( 51 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1687 Expression of HIF-1α、VEGF and MMP-9 in Non-Small Cell Lung Cancer LI Ai-ke, LI Qing-shan, LIN Ping-ping,et al DOI: 10.3969/j.issn.1671-7171.2017.09.007 【Objective】To investigate the expression of hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) in non-small cell lung cancer (NSCLC) and explore their clinical applications.【Methods】The expression of HIF-1α, VEGF and MMP-9 was measured by immunohistochemistry in 91 cases of NSCLC and corresponding adjacent normal tissues. The relationship between these three factors and the clinicopathological parameters of NSCLC was analyzed.【Results】The expression of HIF-1α, VEGF and MMP-9 in cancer tissue of NSCLC was higher than that in adjacent normal tissues (P＜0.05). The positive rates of HIF-1α, VEGF and MMP-9 in low differentiated NSCLC were higher than those in moderate and highly differentiated NSCLC (P＜0.05). The positive rates of HIF-1α, VEGF and MMP-9 in Ⅲ~Ⅳ stage were higher than those in Ⅰ~Ⅱ stage (P＜0.05). The positive rates of HIF-1α, VEGF and MMP-9 in NSCLC with lymph node metastasis were higher than those in non-lymph node metastasis (P＜0.05). The expression of HIF-1α, VEGF and MMP-9 in NSCLC tissue was not correlated with age, sex, tumor diameter and pathological type. The positive expression of HIF-1α, VEGF and MMP-9 in NSCLC was positively related with one another (P＜0.05).【Conclusion】HIF-1α, VEGF and MMP-9 promoted the occurrence and development of NSCLC; the combined detection of these three factors may provide a more valuable prognosis evaluation and target therapy of NSCLC. 2017 Vol. 34 (9): 1687-1690 [Abstract] ( 63 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1691 Effect and the Underlying Mechanism of miRNA-200a in Regulating TGF-β1-induced Endothelial-mesenchymal Transition of Human Aortic Endothelial Cells XIONG Ying, SUN Yan, LI Ying,et al DOI: 10.3969/j.issn.1671-7171.2017.09.008 【Objective】To investigate the effect and the underlying mechanism of miRNA(miR)-200a in regulating TGF-β1-induced endothelial-mesenchymal transition (EndMT) of human aortic endothelial cells (HAVC) by targeting inhibition of high-mobility group protein box1, (HMGB1) .【Methods】1. The expression of miR-200a in human aortic endothelial cell lysates (HAEC) was detected under different concentrations of TGF-β1. 2. The expressions of platelet endothelial cell adhesion molecule-1 (CD31), endothelium-cadherin (VE-cadherin), fibroblast-specific protein-1 (FSP-1) andα-smooth muscle actin (α-SMA) were determined after miR-200a mimics/mimic NC transfection occurred in HAEC with or without TGF-β1 treatment. 3. The expression levels of HMGB1 in miRNA-200a mimics/mimic NC-transfected HAECs that were and were not treated with TGF-β1 were determined. 4. The binding relationship between miR-200a and HMGB1 3'UTR was detected by luciferase assay. 5. The protein expressions of CD31, VE-cadherin, FSP-1 and α-SMA in HAEC transfected with miR-200a mimics and HMGB1 alone or in combination were detected.【Results】1. TGF-β1 inhibited the expression of miR-200a(P＜0.01). 2. miR-200a inhibited TGF-β1-induced EndMT, up-regulated CD31(P＜0.01) and VE-cadherin(P＜0.001), while down- regulating FSP-1 (P＜0.001) and α-SMA (P＜0.01) expression. 3. miR-200a inhibited the expression of HMGB1(P＜0.001), while TGF- β1 promoted the expression of HMGB1(P＜0.001). 4. miR-200a binded to the 3'UTR region of HMGB1. 5. HMGB1 attenuated miR-200a inhibition of EndMT and down-regulated the expression of CD31(P＜0.01) and VE-cadherin(P＜0.01), while up-regulated the expression of α-SMA(P＜0.01) and FSP-1(P＜0.001).【Conclusion】The transfection of HMGB1 plasmid promoted the EndMT process of human aortic endothelial cell.The transfection of miR-200a inhibited the expression of HMGB1 and inhibited EndMT of human aortic endothelial cell induced by TGF-β1.Therefore miRNA(miR)-200a targets the inhibition of high-mobility group protein box1, (HMGB1) in regulating TGF-β1-induced endothelial-mesenchymal transition. 2017 Vol. 34 (9): 1691-1696 [Abstract] ( 52 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 1697 Clinical Observation of Oxaliplatin Combined with 5-fluorouracil Concurrent Intensity-modulated Radiotherapy in Patients with Locally Advanced Nasopharyngeal Carcinoma HAO Jie, QI Hong-zhi, YANG Yu-gang,et al DOI: 10.3969/j.issn.1671-7171.2017.09.009 【Objective】To evaluate the efficacy of oxaliplatin combined with 5-fluorouracil concurrent intensity-modulated radiotherapy (IMRT) for locally advanced nasopharyngeal carcinoma (NPC).【Methods】Eighty-two patients with locally advanced nasopharyngeal carcinoma who were treated in our hospital from May 2012 to May 2014 were randomly divided into observation group (41 cases) and control group (41 cases). The two groups of patients were treated with three-dimensional conformal intensity-modulated radiation therapy(IMRT). The control group was treated with cisplatin on the basis of IMRT. The observation group received oxaliplatin combined with 5-fluorouracil on the basis of IMRT. The two groups were treated 21 d for 1 cycle, continuous 2~4 cycles. The short-term efficacy of the two groups, the local recurrence, distant metastasis and survival in 1 and 3 years, and the occurrence of side effects were compared.【Results】The total effective rate in the two groups was 100%, there was no statistically significant difference between the two groups (P＞0.05); 1-year local recurrence rate showed no significant difference (P＞0.05); 3-years local recurrence rate of the observation group was lower than that of the control group (P＜0.05); 1-year distant metastasis rate of the two groups showed no significant difference (P＞0.05); 3-year distant metastasis rate of the observation group was lower than that of the control group (P＜0.05); gastrointestinal reaction, liver function, renal function and oral mucosa reaction rate of the two groups had no significant difference (P＞0.05); 1-year survival rate of the two groups had no significant difference (P＞0.05); 3-year survival rate of the observation group was higher than that of the control group (P＜0.05).【Conclusion】Oxaliplatin combined with 5-fluorouracil concurrent intensity-modulated radiotherapy is effective in the treatment of locally advanced nasopharyngeal carcinoma. It can reduce local recurrence and metastasis, and prolong the survival time,which is worthy of clinical application. 2017 Vol. 34 (9): 1697-1699,1702 [Abstract] ( 48 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 1700 Correlation between Nutritional Risk, Corresponding Nutritional Support and Short-term Prognosis and Long-term Survival Time in Patients with Gastric Cancer LI Jin DOI: 10.3969/j.issn.1671-7171.2017.09.010 【Objective】To investigate the relationship between nutritional risk, nutritional support and short-term prognosis and long-term survival time in patients with gastric cancer.【Methods】Ninety-five cases of gastric cancer patients were given immediate assessment of nutritional risk after admission, and the status of nutritional support was recorded. The relationship between preoperative nutritional risk, postoperative nutritional support and complications and short-term efficacy was analyzed.【Results】There were 66.32% cases of nutritional risk in 95 patients with gastric cancer (63/95). Among them, 43 patients received nutritional support, accounting for 68.25% (43/63).The nutritional support was performed through parenteral nutrition support (PN) in 18 patients (28.57%), PN and enteral nutrition support (EN) in 25 patients (39.68%);The incidence of complications in patients with nutritional risk of gastric cancer was 42.86%.The mortality rate of 3 year was 31.75%, and 3-year recurrence rate of tumor metastasis was 36.51%, which were higher than those without nutritional risk, 21.88%, 12.5%, 15.63%, and the difference was significant (P＜0.05).Patients with nutritional risk and nutritional support got higher objective relief rate than those with non nutritional supporters.The complication rate, the 3 year mortality rate, the 3 year recurrence and metastasis rate were lower than those with nutritional risk but without nutritional support, and the difference was significant (P＜0.05).【Conclusion】Preoperative nutritional risk assessment and nutritional support can significantly reduce the incidence of complications and improve prognosis in patients with gastric cancer. 2017 Vol. 34 (9): 1700-1702 [Abstract] ( 50 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 1703 Expression and Significance of IL-17 mRNA and IFN-γ mRNA in Rat Models of Endotoxin Induced Uveitis DU Hao, ZHANG Meng, HU Zhi-tao DOI: 10.3969/j.issn.1671-7171.2017.09.011 【Objective】To analyze the expression of interleukin-17 (IL-17) and interferon gamma (IFN- γ) messenger RNA (mRNA) in eyes of rats with endotoxin induced uveitis.【Methods】Forty SD rats were randomly divided into the blank control group (n=10) and the model group (n=30). Rats in the model group were treated with endotoxin to induce uveitis, and 5 rats were sacrificed at 6 h, 12 h, 18 h, 24 h, 48 h, 60 h, respectively;the control group was injected with phosphate buffered saline (PBS), and 10 rats were sacrificed after 24h. Uveal retina of all of them were taken from the both eyes. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the levels of IL-17 mRNA and IFN- γ mRNA of ocular tissues in the two groups. The inflammation of the eye tissue was observed under microscope and scored according to relevant criteria.【Results】① The ocular inflammation score in the model group at different time was higher than that in blank control group (P＜0.05);in the model group, the inflammatory scores were increased successively after 6 h, 12 h and 18 h (P＜0.05), and reached the peak at 18 h, then decreased successively at 24 h, 48 h, 60 h(P＜0.05). ② The IL-17 mRNA and IFN-γ mRNA levels in the model group at different times were higher than those in the blank control group (P＜0.05); levels of IL-17mRNA and IFN-γ mRNA in the model group at 6 h, 12 h, 18 h increased successively (P＜0.05), and reached the peak at 18 h , then successively decreased at 24 h, 48 h, 60 h (P＜0.05).【Conclusion】The expression levels of IL-17 mRNA and IFN-γ mRNA in the ocular tissues of uveitis rats are up regulated, which are consistent with the extent of lesions; IL-17, IFN-γ may be involved in the pathogenesis of uveitis. 2017 Vol. 34 (9): 1703-1705 [Abstract] ( 46 ) HTML (1 KB)  PDF  (0 KB)  ( 14 ) 1706 Comparison of Propofol and Propofol Combined with Fentanyl in Painless Induced Abortion XIE Bin DOI: 10.3969/j.issn.1671-7171.2017.09.012 【Objective】To compare the safety and effectiveness of propofol and propofol combined with fentanyl in painless induced abortion.【Methods】Sixty cases of patients with painless induced abortion were randomly divided into the propofol anesthesia group ( the Group A ) and the propofol plus fentanyl anesthesia group ( the Group B). The changes of heart rate (HR), mean arterial pressure (MAP) and oxygen saturation (SpO2) were observed before and after anesthesia in the two groups. The drug efficiency evaluation, cervical looseness, postoperative incidence rate of adverse reactions were compared in the two groups.【Results】The changes of HR, MAP and SpO2 in the A group were higher than those of the group B (P＜0.05). The pharmacodynamic initial play time and duration of action of the group A were longer than those of the group B (P＜0.05). The drug total efficiency of the group A was lower than that of the group B[30%(9/30) vs. 83.33%(25/30)](P＜0.05). The postoperative incidence rate of adverse reactions in the group A was higher than that in the group B(P＜0.05).【Conclusion】For painless induced abortion, the effect of fentanyl + propofol anesthesia is more satisfactory, and it is worthy of clinical promotion. 2017 Vol. 34 (9): 1706-1708 [Abstract] ( 47 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1708 Thyroid Adenoma and Nodular Goiter in Clinical Pathological Diagnosis Research LIU Xin-ya, LI Guo, TANG Zhi-rong,et al DOI: 10.3969/j.issn.1671-7171.2017.09.013 【Objective】To analyze the clinical pathological features and fine needle aspiration diagnosis of thyroid adenoma (TA) and single nodular goiter (NG).【Methods】Data of 734 cases of thyroid diseases confirmed by operation and pathology in our hospital were collected. According to the latest pathological classification standard of WHO, the re analysis and analysis were mainly based on the pathological morphology.【Results】The results of the re examination showed that in 734 cases of thyroid diseases, nodular NG cases 178, TA cases 368, thyroid cancer 84cases, thyroiditis 40cases, diffuse toxic goiter 62 cases, Lymphoma 2 cases; compared with the prior examination, nodular NG increased in 18 cases, while TA reduced in 18 cases, the rest remained unchanged; the accuracy of intraoperative frozen section diagnosis was 100%, and the accurate rate was 88.24%. The accuracy of fine needle aspiration diagnosis was low, only 77.78% (70/90), whereas the diagnosis of thyroid follicular carcinoma was the most difficult.【Conclusion】Most of TA and single nodular NG have cystic changes, and the pathological differential diagnosis is difficult, therefore the accuracy of fine needle aspiration biopsy is not so high.Thus an accurate diagnosis should be made in combination with the history and laboratory findings. 2017 Vol. 34 (9): 1708-1710 [Abstract] ( 103 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 1711 Clinical Analysis of Modified Radical Cystectomy Combined with Orthotopic Ileal Neobladder for Invasive Bladder Cancer in Male Patients WANG Zhi, SHEN Kai, LIAO You-gang DOI: 10.3969/j.issn.1671-7171.2017.09.014 【Objective】To investigate the clinical efficacy of extraperitoneal modified retrograde cystectomy combined with orthotopic ileal neobladder in the treatment of invasive bladder cancer in men.【Methods】In our hospital, 78 cases of invasive bladder cancer in male patients were randomly divided into observation group (39 cases) and control group (39 cases).They were treated with extraperitoneal modified retrograde cystectomy combined with orthotopic ileal neobladder and conventional cystectomy combined with orthotopic ileal neobladder. The blood loss, operation time, gastrointestinal function recovery time and postoperative hospital stay were compared between the two groups, and the urine control during the day and night 3 months and 6 months after operation in the two groups was also observed.The urine flow dynamics and complications were compared between the two groups at 6 months after operation.【Results】The intraoperative blood loss, operation time, gastrointestinal function recovery time and postoperative hospital stay in the observation group were significantly smaller than those in the control group (P＜0.05);day and night urinary continence had no significant difference in the two groups 3 months and 6 months after the operation (P＞0.05). However, the urinary continence during the daytime was better than that in the night, and the difference was statistically significant (P＜0.05).There was no significant difference in urodynamic parameters between the two groups at 6 months after operation (P＞0.05).The total incidence of complications in the observation group was 5.13%, significantly less than that in the control group 20.51% , and the difference was statistically significant (P＜0.05).【Conclusion】The treatment of male invasive bladder cancer with modified extraperitoneal cystectomy combined with orthotopic ileal neobladder is of remarkable clinical efficacy, which can effectively improve the patient's urine control with less complications. The therapy is safe and reliable and worthy of clinical application. 2017 Vol. 34 (9): 1711-1714 [Abstract] ( 28 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1714 Analysis of Clinical Features and Detection Rate of Colon Cancer in 1595 Cases of Colorectal Polyps ZHANG Yu, ZHOU Wei, LIU Hua-zhu,et al DOI: 10.3969/j.issn.1671-7171.2017.09.015 【Objective】To analyze the clinical features of colorectal polyps and detection of colon cancer in 1595 cases.【Methods】The clinical general data of 1595 cases with colorectal polyps (1805 polyps) who underwent colonoscopy were collected and analyzed.【Results】 There was no significant difference in age distribution between patients with colorectal polyps of different gender (P＞0.05). The proportion of patients with colorectal polyps increased with the increase of age, and the proportion in 41~50 years old was significantly higher. Compared with patients ≤ 40 years old (P＜0.05), the proportion of patients ＞ 60 years old was the highest. Among the 1805 polyps, there were 993 (55.01%) adenomatous polyps,which was more that the non adenomatous polyps 812 (44.99%)(P＜0.05). The polyps in ascending, descending, transverse and sigmoid colon and rectum were adenomatous polyps mainly, while polyps at ileocecus were non adenomatous polyps mainly, there were significant differences between the two (P＜0.05). There were 304 cases with colon cancer (312 polyps) detected, and the detection rate was 19.06%. There were significant differences in age, polyp size, pathological types and polyp location between patients with canceration of colorectal polyps and those without canceration (P＜0.01).【Conclusion】The risk of colorectal polyps increases with the increase of age. The adenomatous polyps in the ascending, descending, transverse and sigmoid colon and rectum and non adenomatous polyps at ileocecus are common. Advanced age, large polyps, adenomatous polyps in the ascending colon and rectum can increase the risk of colon cancer. 2017 Vol. 34 (9): 1714-1716 [Abstract] ( 36 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 1717 A Control Study on the Efficacy and Safety of Morinda Officinalis Oligose Capsules ersus Escitalopram in Patients with Depression CHEN Jing-yu, ZHANG Tao, LI Ying, et al DOI: 10.3969/j.issn.1671-7171.2017.09.016 【Objective】To compare the efficacy and safety between Morinda officinalis oligose capsule and Escitalopram in the patients with depression.【Methods】An open-label controlled clinical trial was conducted. A total of 76 cases of outpatients with depression were randomly divided into a study group (Morinda officinalis oligose capsule) with 38 cases and a control group (Escitalopram) with 38 cases. The dose of study group was 300mg or 600mg/d of morinda officinalis oligose capsule, while the control group received 10mg or 20mg/d of escitalopram. The treatment in both groups lasted 6 weeks. Hamilton Depression Rating Scale 17-Item (HAMD-17), TCM Kidney Deficiency Quantitative Score Form and Clinical Global Impression Scale (CGI) were used to evaluate the efficacy, and the assessment of adverse events, physical examination, laboratory tests and ECG were evaluated to determine safety.【Results】A total of 35 cases had completed a 6-week treatment observation in the study group, while 34 cases did so in the control group. The scores of HAMD-17 in the observation group and the control group at the 2nd,4th and 6th week were significantly lower than those before treatment (all P＜0.01). There was no significant difference in the HAMD-17 scores between the observation group and the control group at the 2nd,4th and 6th week after treatment (P＞0.05). The total score of HAMD-17 in the observation group and the control group were both significantly reduced at the end of 6 weeks, and score-reducing rate was (57.82 ± 16.93) % and (60.21 ± 15.40) %, respectively. The difference was not statistically significant (t=0.243,P=0.809). The clinical total response rates of two groups were 71.42% (25/35) and 78.37% (29/37), respectively. The difference was not statistically significant; TCM symptoms effective rates of the observation group and the control group were 68.57% and 70.27%, respectively. The difference was not statistically significant (P=0.876). The incidence rates of drug-related adverse reactions were 25.71% (9/35) and 24.32% (9/37), respectively. The difference between the two groups was not statistically significant (χ2= 0.019,P=0.892).【Conclusion】Morinda officinalis oligose capsule and escitalopram have equivalent efficacy in the treatment of mild to moderate depression and have mild adverse reactions and good safety. 2017 Vol. 34 (9): 1717-1719,1723 [Abstract] ( 77 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 1720 Clinical Value of Transvaginal Color Doppler Sonography plus Human Epididymis Protein 4 Test for Endometrial Cancer Diagnosis LIU Qun, SU Wen-hui, ZHANG Qiong-zhen DOI: 10.3969/j.issn.1671-7171.2017.09.017 【Objective】To study the clinical value of transvaginal color Doppler sonography(TV-CDS)plus human epididymis protein 4(HE4)test for endometrial cancer diagnosis.【Methods】Clinical information of 206 patients with irregular vaginal bleeding, who were examined in our hospital from March,2014 to March, 2017, were studied and divided into the endometrial cancer (EC)group and the benign diseases(BD)group. The uterine morphology was observed, the thickness and blood flow of endometrium were measured and the endometrial volume was calculated by TV-CDS examination. The serum HE4 was detected by Elisa assay.【Results】The uterine maximum diameter, endometrial thickness and volume of the EC group were significantly larger than those of the BD group(P＜0.05). The level of serum HE4 in the EC group was significantly higher than that in the BD group(P＜0.05). The accuracy rates of TV-CDS plus serum HE4 test for diagnosis of endometrial cancer and benign diseases were 94.02% and 92.13%,respectively, which were significantly higher than those of either method alone(P＜0.05). The false negative rate and the false positive rate were 5.98% and 7.87%, which were significantly lower than those of either method alone(P＜0.05). The sensitivity of TV-CDS plus serum HE4 for diagnosing endometrial cancer was 94.02%, the specificity was 4.38%, positive predictive value was 95.65%, negative predictive value was 92.31% and Youden index was 0.884,which were all significantly higher than those of TV-CDS or serum HE4 test alone(P＜0.05).【Conclusion】TV-CDS plus serum HE4 test can efficiently improve the diagnostic efficacy for endometrial cancer more than using either of the two methods alone. Therefor it is a useful tool for clinical practice. 2017 Vol. 34 (9): 1720-1723 [Abstract] ( 45 ) HTML (1 KB)  PDF  (0 KB)  ( 9 ) 1724 Comparative Study of Multi-slice Spiral CT Coronary Calcification Score and Dynamic Electrocardiogram Monitoring the Early Diagnosis of Coronary Artery Disease ZHU Da-hua, YING Long-lin DOI: 10.3969/j.issn.1671-7171.2017.09.018 【Objective】Catheter coronary angiography was set as the "gold standard" to investigate the value of multi-slice spiral CT coronary calcification score and dynamic electrocardiogram (ECG) monitoring in the early diagnosis of coronary artery disease.【Methods】A total of 55 cases of patients with coronary heart disease in our hospital from April 2013 to January 2014 were collected for the study. All patients underwent CT angiography (CTA, for multi-slice spiral CT coronary calcification score) and dynamic ECG detection. The results were compared with those of CAG "gold standard". The sensitivity and specificity of the two different diagnostic methods were analyzed.【Results】The sensitivity of dynamic ECG monitoring was 97.06% (33/34); the specificity was 90.48% (19/21), and the positive predictive value was 94.29% (33/35). The sensitivity of multi-slice spiral CT coronary angiography was 100% (32/32); the specificity was 86.96% (20/23), and the positive predictive value was 91.43% (32/35). There were no statistical differences between the two groups (P＜0.05).【Conclusion】Both dynamic electrocardiogram and multi-slice spiral CT coronary calcification score are highly accurate for early diagnosis of coronary heart disease. Dynamic ECG monitoring, however, is more practical in clinical settings due to its simplicity, repeatability, and non-invasive nature. 2017 Vol. 34 (9): 1724-1726 [Abstract] ( 54 ) HTML (1 KB)  PDF  (0 KB)  ( 14 ) 1726 Risk Factors of Nasopharyngeal Ulcer after Radical Intensity Modulated Radiation Therapy for Nasopharyngeal Carcinoma HUANG Gang, WANG Jun, LI Yan DOI: 10.3969/j.issn.1671-7171.2017.09.019 【Objective】To investigate the risk factors of nasopharyngeal ulcer after radical intensity modulated radiation therapy (IMRT) for nasopharyngeal carcinoma.【Methods】A retrospective analysis of 80 nasopharyngeal carcinoma patients (ulcer group) with nasopharyngeal ulcer 1 year after radical IMRT was performed in our hospital from August 2014 to August 2015.At the same time, clinical data of 80 patients with nasopharyngeal carcinoma (non ulcer group) without nasopharyngeal ulcer 1 year after radical IMRT was also analyzed(P＜0.05).【Results】There was no significant difference between the two groups in age, sex, education level, N stage, drinking status and so on (P＞0.05). Single factor analysis showed that T staging, chemotherapy, no nasal irrigation, nasopharynx dose＞72 Gy, no use of antibiotics, smoking, malnutrition were the dangerous factors of the incidence of nasopharyngeal ulcer after radical IMRT in nasopharyngeal carcinoma patients (P＜0.05).【Conclusion】T stage, chemotherapy, nasopharynx dosage ＞72 Gy, smoking, malnutrition and other factors are closely related to the incidence of nasopharyngeal ulcer after radical IMRT in nasopharyngeal carcinoma patients, which should be paid attention to. 2017 Vol. 34 (9): 1726-1728 [Abstract] ( 42 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 1729 Study of COX-2 and Ki-67 Expression in Hepatobiliary Calculus with Cholangiocarcinoma CHEN Chen, YANG Xi-wen, LUO Liang, JIANG Bo,et al DOI: 10.3969/j.issn.1671-7171.2017.09.020 【Objectives】To explore the expression of COX-2 and Ki-67 in patients of hepatobiliary calculus with Cholangiocarcinoma (HCWC) and their clinical significance. Relationship between the expression of COX-2、Ki-67 and the onset and progress of HCWC was investigated to lay an experimental base for the prevention and treatment of HCWC.【Methods】Eighteen cases (n=18) of normal bile duct tissue from operations of hemangiomas of liver or liver trauma were used as the control (group A);fifty-five (n=55) cases of the bile duct tissue surrounding calculus of intra-hepatic were selected as group B, and forty-four (n=44) cases of tumor tissue of HCWC (group C) were randomly sampled and collected between January 2000 to December 2016 in the Department of Hepatobiliary Surgery of Hunan Provincial People's Hospital. Immunohistochemistry was used to detect the expression of COX-2 and Ki-67 as well as their expression in cholangiocarcinoma in correlation with clinicopathological features. The results were statistically analyzed.【Results】In group A,B,C, the positive rates of the expression of COX-2 and Ki-67 were 5.56%,32.72%, 70.45%, and 0, 27.27%,79.55%, respectively. The positive rate of the expression of COX-2 and Ki-67 in the carcinoma tissue of HCWC was significantly higher compared with the control group (P＜0.05); the positive rate of COX-2 and Ki-67 expression in poorly differentiated HCWC cases was higher compared with highly differentiated cases (P＜0.05); the positive rate of COX-2 and Ki-67 in extra-hepatic or lymphatic metastasis HCWC cases was higher compared with cases without metastasis (P＜0.05). There was no significant difference in the expression rate of COX-2 and Ki-67 between the different age groups, sex, duration, tumor size and pathological type of group C (P＞0.05). The positive expression rate of Ki-67 in COX-2 positive cases of group C was 90.32% (28/31). Spearman-related analysis showed a strong positive correlation between the expression of COX-2 and Ki-67 in HCWC(r=0.413,P=0.005).【Conclusions】 There is a significantly positive correlation between the expression of COX-2 and Ki-67 in HCWC. The high expression of COX-2 and Ki-67 play a certain role in the occurrence and progress of HCWC. 2017 Vol. 34 (9): 1729-1732 [Abstract] ( 96 ) HTML (1 KB)  PDF  (0 KB)  ( 8 ) 1733 Pregnancy Outcome Analysis of in Vitro Fertilization-Embryo Transfer in Elderly Women of Different Ages DENG Zhao-hui, YU Hong, PAN Xiang-yang, et al DOI: 10.3969/j.issn.1671-7171.2017.09.021 【Objective】To investigate the influence of age of elderly female patients on the outcome of in vitro fertilization-embryo transfer (IVF-ET).【Methods】In this retrospective analysis, a total of 1559 elderly infertility patients who received fresh embryo transfer between Jan 2013 and Dec 2015 were analyzed . The elderly patients, with a total of 1559 cycles, were divided according to the ageinto 3 groups: ages 35~39 (group A, 914 cycles), ages 40~44 (group B, 542 cycles), and ages ≥45 (group C, 103 cycles). Results of insemination rate, clearage rate, rate of high quality embryo, cycle cancelation rate , implantation rate, pregnancy rate, live birth rate and abortion rate were compared among the three groups.【Results】There was no significant difference in the insemination rate, clearage rate and the rate of high quality embryo among three groups (P＞0.05). In the order of group A, B to C the cycle cancelation rate (27.13%、43.73%、63.11%, respectively), implantation rate (27.63%、13.29%、1.39%, respectively),pregnancy rate (46.83%、29.51%、2.63%, respectively), live birth rate (33.78%、15.41%、0%, respectively) and abortion rate (22.44%、38.67%、100%, respectively) increased or decreased; there were significant differences (P＜0.05) between each group.【Conclusion】In elderly infertility women, the age of the patients has an important effect on the success of IVF-ET. 2017 Vol. 34 (9): 1733-1735 [Abstract] ( 45 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1736 Distribution and Risk Factors of Type H Hypertension in the Elderly in Deyang Area PU Cheng-kun, LI Bin-bin, LIU Yun-bo,et al DOI: 10.3969/j.issn.1671-7171.2017.09.022 【Objective】To investigate the epidemiological characteristics and risk factors of type H hypertension in the elderly in Deyang.【Methods】Two thousand and one hundred residents more than 60 years in the city of Deyang were selected for the questionnaire. Blood pressure measurement and laboratory were investigated using stratified random sampling principle to understand the incidence of type H hypertension, and the risk factors were analyzed by multivariate Logisitic regression.【Results】The prevalence of type H hypertension in the urban elderly in Deyang was 32.71% (687/2100). In men and women with type H hypertension, there was significant difference in smoking rate, alcohol consumption rate, addicted to meat rate, fasting blood glucose (GLU), uric acid (UA) and creatinine (Cr) (P＜0.05).Univariate analysis showed that the onset of type H hypertension in the elderly was related to the duration of hypertension, smoking rate , addicted to meat and salt rate, systolic blood pressure (SBP), diastolic blood pressure (DBP), and plasma homocysteine (Hcy). Multivariate Logistic regression analysis showed that smoking and high Hcy were independent risk factors for type H hypertension in elderly patients.【Conclusion】The detection rate of type H hypertension in elderly people over 60 years of age in Deyang is higher, which is closely related to smoking, addicted to meat rate and high Hcy. These are independent risk factors for the disease. 2017 Vol. 34 (9): 1736-1738 [Abstract] ( 81 ) HTML (1 KB)  PDF  (0 KB)  ( 7 )

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