|
|
|
| Expression and Significance of IL-17 mRNA and IFN-γ mRNA in Rat Models of Endotoxin Induced Uveitis |
| DU Hao, ZHANG Meng, HU Zhi-tao |
| Department of Ophthalmology, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China |
|
|
|
|
Abstract 【Objective】To analyze the expression of interleukin-17 (IL-17) and interferon gamma (IFN- γ) messenger RNA (mRNA) in eyes of rats with endotoxin induced uveitis.【Methods】Forty SD rats were randomly divided into the blank control group (n=10) and the model group (n=30). Rats in the model group were treated with endotoxin to induce uveitis, and 5 rats were sacrificed at 6 h, 12 h, 18 h, 24 h, 48 h, 60 h, respectively;the control group was injected with phosphate buffered saline (PBS), and 10 rats were sacrificed after 24h. Uveal retina of all of them were taken from the both eyes. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the levels of IL-17 mRNA and IFN- γ mRNA of ocular tissues in the two groups. The inflammation of the eye tissue was observed under microscope and scored according to relevant criteria.【Results】① The ocular inflammation score in the model group at different time was higher than that in blank control group (P＜0.05);in the model group, the inflammatory scores were increased successively after 6 h, 12 h and 18 h (P＜0.05), and reached the peak at 18 h, then decreased successively at 24 h, 48 h, 60 h(P＜0.05). ② The IL-17 mRNA and IFN-γ mRNA levels in the model group at different times were higher than those in the blank control group (P＜0.05); levels of IL-17mRNA and IFN-γ mRNA in the model group at 6 h, 12 h, 18 h increased successively (P＜0.05), and reached the peak at 18 h , then successively decreased at 24 h, 48 h, 60 h (P＜0.05).【Conclusion】The expression levels of IL-17 mRNA and IFN-γ mRNA in the ocular tissues of uveitis rats are up regulated, which are consistent with the extent of lesions; IL-17, IFN-γ may be involved in the pathogenesis of uveitis.
|
|
Received: 22 January 2017
|
|
|
|
|
|
[1]王影,李洋,毕宏生,等.实验性自身免疫性葡萄膜炎炎性因子表达的动态观察[J].中华实验眼科杂志,2013,31(7):647-652.
[2]Ke Y,Sun D,Jiang G,et al.IL-22-induced regulatory CD11b+ APCs suppress experimental autoimmune uveitis [J].J Immunol,2011,187(5):2130-2139.
[3]侯彬,沈琳,王红,等.白细胞介素-17、白细胞介素-4及γ干扰素在实验性自身免疫性葡萄膜炎中的表达[J].中华眼底病杂志,2014,30(4):386-389.
[4]邹文军,武志峰,项晓丽,等.人类白细胞抗原-B27阳性急性前葡萄膜炎患者外周血中Th细胞亚群的表达及意义[J].中华实验眼科杂志,2012,30(12):1118-1121.
[5]Takeuchi M,Usui Y,Okunuki Y,et al.Immune responses to interphotoreceptor retinoid-binding protein and S-antigen in Behcet's patients with uveitis [J].Invest Ophthalmol Vis Sci,2010,51(6):3067-3075.
[6]钱丽丽,徐亚茹,胡芳,等.Th1、Th17细胞相关因子在实验性自身免疫性葡萄膜炎大鼠中的表达及作用[J].眼科新进展,2015,35(10):918-920.
[7]Sugita S,Kawazoe Y,Imai A,et al.Role of IL-22- and TNF-α-producing Th22 cells in uveitis patients with Behcet's disease[J].J Immunol,2013,190(11):5799-5808.
[8]肖青,粘红,魏瑞华,等.肽聚糖对树突细胞分泌促炎性细胞因子以及实验性自身免疫性葡萄膜炎辅助性T细胞17的影响[J].中华眼底病杂志,2013,29(6):600-604.
[9]陈琳,粘红,魏瑞华,等.TLR7激动剂CL097对实验性自身免疫性葡萄膜炎中Th17细胞活性的促进作用[J].中华实验眼科杂志,2015,33(4):294-299.
[10]王延梅.川芎嗪对大鼠视网膜缺血再灌注损伤的保护作用[J].医学临床研究,2013,30(5):910-911.
[11]孟祥达,颜华.白杨素对小鼠实验性自身免疫性葡萄膜炎的抑制作用及其机制[J].中华实验眼科杂志,2015,33(7):588-593.
[12]蔡莉,高玮,王颖维,等.IFN-α 治疗白塞病葡萄膜炎的新进展[J].中华眼视光学与视觉科学杂志,2016,18(7):446-448.
[13]滕达,毕宏生,崔彦,等.葡萄膜炎相关细胞因子的研究进展[J].中国实用眼科杂志,2013,31(11):1382-1386.
[14]Caraballo JN,Snyder MR,Johnston PB,et al.Vitreoretinal lymphoma versus uveitis: Cytokine profile and correlations[J].Ocul Immunol Inflamm,2014,22(1):34-41.
[15]袁立飞,张晓敏,李光达,等.间充质干细胞对大鼠实验性自身免疫性葡萄膜炎视网膜中白细胞介素17表达的影响[J].中华实验眼科杂志,2012,30(5):392-396. |
| [1] |
. [J]. JOURNAL OF CLINICAL RESEARCH, 2017, 34(6): 1240-1242. |
|
|
|
|
2017, Vol. 34 
