HMGB1/RAGE在肝缺血再灌注大鼠的表达及奥曲肽的预处理作用

doi:10.3969/j.issn.1671-7171.2017.09.004

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Abstract 【Objective】To investigate the expression of HMGB1/RAGE in hepatic ischemia-reperfusion injury (HIRI) and the preconditioning effect of octreotide (Oct) in rats.【Methods】Seventy-two healthy adult male SD rats were randomly divided into three groups equally (n=24): sham operation group(group S), ischemia reperfusion group (group I/R), and octreotide group(group Oct). 30 minutes before operation, the Group Oct received intraperitoneal injection of saline containing Oct 0.25 mg/kg, while the group S and the group I/R were intraperitoneally injected with the same amount of normal saline. HIRI model was established by Pringle 's maneuver method. Blood sampling was collected to detect serum alanine transaminase (ALT) and the change of high mobility group box protein 1 (HMGB1) by immunohistochemistry at liver ischemia 30 min (T₁), reperfusion time was 1 h (T₂), 6 h (T₃) and 12 h (T₄). The liver tissue was taken at 12 h after reperfusion, the expression of HMGB1 was detected by immunohistochemistry; The expression of mRNA and protein levels of HMGB1 and advanced glycation end products receptor (RAGE) were detected by real-time quantitative PCR and Western blot respectively.【Results】Serum ALT levels increased from T₁ to T₄ in the group I/R and the group Oct, all of them were higher than those in the group S, the I/R group was significantly higher than the Oct group, the difference was statistically significant (P＜0.05); The level of HMGB1 increased from T₁ to T₃, T₃ peaked and T₄ began to decrease, but all were higher than those in group S. The I/R group was significantly higher than the Oct group, the difference was statistically significant (P＜0.05). The HMGB1 positive cells showed the cytoplasm of nucleus and cytoplasm were brownish yellow. The positive expression of group I/R and group Oct were (6.8 ± 1.2), (4.1 ±1.5), significantly higher than that of group S (3.2 ± 1.3), and the I/R group was significantly higher than the Oct group, the difference was statistically significant (P＜0.05). Compared with group S, the expression of HMGB1, RAGE, mRNA and protein in I/R liver tissue increased significantly (P＜0.05); Compared with group I/R, the expression of HMGB1, RAGE and mRNA in Oct group decreased significantly after pretreatment with Oct (P＜0.05).【Conclusion】Oct preconditioning has obvious protective effect on HIRI in rats, which may be related to the inhibition of the expression of HMGB1 and RAGE.

Key words： Liver/IN Liver Diseases/PC Reperfusion Injury Octreotide/PD High Mobility Group Proteins/ME Glycosylation End Products, Advanced/ME

Received: 19 January 2017

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R364.12

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http://www.jcr.net.cn/EN/10.3969/j.issn.1671-7171.2017.09.004 OR http://www.jcr.net.cn/EN/Y2017/V34/I9/1677

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