厄多司坦对急性肺损伤小鼠肺内Nrf2表达的影响

doi:10.3969/j.issn.1671-7171.2017.09.003

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Abstract 【Objective】To investigate the effect of erdosteine on the expression of antioxidant transcription factor Nrf2 in lung of acute lung injury (ALI) mice induced by lipopolysaccharide (LPS).【Methods】The healthy male C57bl/6 mice were randomly divided into control group, ALI group and erdosteine group (n=8). The mouse model of ALI was reproduced by intratracheal injection of LPS (5 mg/kg). Erdosteine (150 mg/kg) was given 30 min before delivery and the mice were sacrificed 12 h later. HE staining was used to observe the morphological changes of lung tissue in mice. The content of malondialdehyde (MDA) and superoxide dismutase (SOD) were measured to reflect the oxidative stress in lung tissue. The expression of Nrf2 in lung tissue was detected by Western blot. Real-time PCR was used to detect the contents of HO-1 and SOD mRNA in lung tissue.【Results】Erdosteine could improve the histopathological changes of lung tissue induced by LPS in ALI mice, decrease the content of MDA in lung tissue and increase the activity of SOD. Erdosteine could also significantly increase the expression of Nrf2 in the nucleus of ALI mice and significantly increase the expression of HO-1 and SOD mRNA.【Conclusion】Erdosteine can alleviate LPS-induced ALI in mice.The mechanism may be due to the enhancement of the nuclear translocation and transcription activity of the antioxidant transcription factor Nrf2 in the lung of ALI mice.

Key words： Lung/IN Thioglycolates/PD Thiophenes/PD Acute Disease Lipopolysaccharides Lung Diseases/CI Oxidative Stress

Received: 14 August 2017

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R563

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	DONG Liang
	ZHOU Yi-qi
	MAO Zhen-yu
	et al

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DONG Liang,ZHOU Yi-qi,MAO Zhen-yu, et al. Effect of Erdosteine on the Expression of Nrf2 in Mice Acute Lung Injury[J]. JOURNAL OF CLINICAL RESEARCH, 2017, 34(9): 1673-1676.

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http://www.jcr.net.cn/EN/10.3969/j.issn.1671-7171.2017.09.003 OR http://www.jcr.net.cn/EN/Y2017/V34/I9/1673

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