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Quick Search     Adv Search 2017 Vol. 34, No. 3 Published: 2017-03-30 Article          Article 417 Study on the Relationship between DNA Damage Repair Gene Polymorphism and 308nm Excimer Laser Treatment Effect for Vitiligo ZHAO Lian-ying, LIU Dong-qing, LIU Bao-guo,et al DOI: 10.3969/j.issn.1671-7171.2017.03.001 【Objective】 To explore the relationship between DNA damage repair gene polymorphism and 308nm excimer laser treatment for vitiligo. 【Methods】 A total of 66 patients diagnosed with vitiligo from July 2015 to July 2016 were selected as the research subjects. All patients received the 308nm excimer laser treatment. Peripheral blood (1 mL) was drawn on the fasting morning before treatment to extract genome DNA. Direct sequencing was used for genotype of two SNP sites (ERCC1 C118T and XPC Lys939Gln). The relationship between DNA damage repair gene polymorphism and laser treatment effect of vitiligo was analyzed. 【Results】 Twenty-six out of sixty-six (39.39%) patients received less than or equal to 4 months treatment. Patients with more than 50% of vitiligo area skin color restoring accounted for 34.85%. The first skin color restoring time for less than or equal one month accounted for 75.76%. ERCC1 C118T rs11615 was correlated with color restoring effect, first color restoring time, and duration of treatment (P<0.05). The higher percentage of CC genotype of ERCC1 C118T SNP showed more efficacy in skin color recovery (restoring area >50%), shortening of first restoring color time (≤1 month), shorter time treatment (≤1 month) comparing to CT+TT genotype of this site (P<0.05). XPC Lys939Gln SNP site had nothing to do with color restoring effect (P>0.05). 【Conclusion】 ERCC1 C118T SNP site is significantly related to 308nm excimer laser treatment effect. Compared to the CT+TT genotype, the CC genotype of ERCC1 C118T SNP is more efficiency in color restoring effect. The XPC Lys939Gln SNP site has little effect on laser treatment effect. 2017 Vol. 34 (3): 417-421 [Abstract] ( 148 ) HTML (1 KB)  PDF  (0 KB)  ( 22 ) 421 Study on the Relationship between Vitamin D and Clinical Symptoms in Children with Autism Spectrum Disorders XU Ning-an, ZHONG Yan, ZHAO Sha,et al DOI: 10.3969/j.issn.1671-7171.2017.03.002 【Objective】To investigate the serum 25-hydroxy vitamin D [25 (OH) D] levels in children with Autism Spectrum Disorders (ASDs) and the relationship with clinical symptoms so as to provide clues and basis for the etiology and treatment of autism. 【Methods】Sixty cases of children with autism diagnosed in Hunan Children's Hospital from June 2014 to June 2016 were selected as the observation group , and other sixty healthy children for normal physical examination were selected as control group in the same period of time . Serum levels of 25 (OH) D of the two groups were determined by enzyme-linked immunosorbent assay (ELISA). Childhood autism Rating Scale (CARS) was used to assess the severity of autism. The levels of 25 (OH) D and CARS score were compared between the two groups. The incidence of sleep disorders and gastrointestinal disorders and their relationship with the level of 25 (OH) D between the two groups were also compared. 【Results】The 25 (OH) D level in the observation group was significantly lower than that in the control group, but it was not significantly associated with the CARS score. The incidence of sleep disorders and gastrointestinal dysfunction in the observation group was significantly higher than those in the control group (P<0.001), but the incidence of two of them was not significantly associated with levels of 25 (OH) D. 【Conclusion】The 25 (OH) D level of the observation group is significantly lower than that of the control, but there is no significant correlation with the severity of ASD, sleep disturbance and gastrointestinal dysfunction. 2017 Vol. 34 (3): 421-423 [Abstract] ( 99 ) HTML (1 KB)  PDF  (0 KB)  ( 15 ) 424 Effects of Growth Hormone on Follicular Development of Patients with Decreased Ovarian Reserve under In-Vitro Fertilization-Embryo Transfer YU Hong, YANG Ling, TANG Hui-zhen,et al DOI: 10.3969/j.issn.1671-7171.2017.03.003 【Objective】To explore the effects of growth hormone(GH)on follicular development in patients with decreased ovarian reserve(DOR)under in-vitro fertilization-embryo transfer (IVF-ET).【Methods】A total of 20 DOR patients received IVF-ET therapy were divided into observation group (11 cases, GH) and control group (9 cases, without GH) according to the wishes of patients. On the day of oocyte retrieval, follicular fluid of single dominant follicle (diameter ≥16 mm) of the two groups was collected , and by Western Blot method, semi quantitative detection of each follicle insulin-like growth factor -1 (IGF-1), IGF-1 receptor (IGF-1R) and GH were conducted; and their correlation with egg quality, embryo quality and the outcome of pregnancy were assessed.【Results】 Total Gonadotropin (Gn) , Gn duration , good embryo rate and cycle cancellation rate of the two groups had no significant difference (P>0.05); the number of dominant follicles on the day of oocyte retrieval , the number of oocytes obtained and MII oocytes of the observation group were significantly higher than those of the control group (P<0.05);The clinical pregnancy rate of the GH group was 25% , while the control group had no clinical pregnancy. GH level of follicular fluid of the two groups was positively correlated with that of IGF-1 and IGF-1R (R=0.55 and 0.58). GH levels of follicular fluid in the observation group were significantly higher than those in the control group (P<0.05); comparing with the level of IGF-1 and IGF-1R, the difference was not statistically significant (P>0.05).IGF-1R and GH levels with MII follicular fluid were higher than those without MII follicular fluid in the same group (P<0.05), and were higher than that of the control group with MII follicular fluid (P<0.05) ; comparison of IGF-1, IGF-1R without MII follicular fluid in the GH group with those in control group with follicle showed no significant difference (P>0.05),but higher than that of the control group without MII follicular fluid.【Conclusion】 GH addition to DOR patients underwent IVF therapy could increase the level of IGF-1, IGF-1R in follicular fluid,and is conducive to improving follicle maturation and follicle qualities. 2017 Vol. 34 (3): 424-427,430 [Abstract] ( 136 ) HTML (1 KB)  PDF  (0 KB)  ( 20 ) 427 Sex Hormone Binding Globulin in Evaluating Abnormal Glucose Metabolism and Insulin Resistance in Patients with Polycystic Ovary Syndrome JIANG Xiu-ying DOI: 10.3969/j.issn.1671-7171.2017.03.004 【Objective】 To investigate the value of sex hormone binding globulin (SHBG) in evaluating abnormal glucose metabolism and insulin resistance (IR) in patients with polycystic ovary syndrome (PCOS). 【Methods】A total of 150 patients with PCOS were divided into the low SHBG (SHBG < 18nmol / L) group (n=70) and normal SHBG (SHBG ≥ 18 nmol / L) group (n=80). Simultaneously, 95 healthy people were selected as the control group. The levels of serum SHBG, luteinizing hormone (LH), follicle stimulating hormone (FSH), fasting plasma glucose (FPG), fasting insulin (FINS), glucose metabolism, and IR in all groups were detected, and the correlation between SHBG and glucose metabolism, IR was analyzed. 【Results】SHBG levels of PCOS patients (including the low SHBG group and normal SHBG group) were significantly lower than that of control group (P<0.05), while the levels of estradiol (E2), testosterone (T), LH, and LH/FSH in PCOS patients were significantly higher than those in the control group. The levels of FINS, triglyceride (TG) and cholesterol (TC) in the low SHBG group and normal SHBG group of PCOS patients were significantly higher than those in the control group (P<0.05). Compared to the normal SHBG group, the insulin resistance index (HOMA-IR), PFG, FINS, TG and TC levels in the low SHBG group were significantly higher (P<0.05), while the insulin sensitivity index (ISI) was significantly lower (P<0.05). Correlation analysis showed that SHBG was negatively correlated with HOMA-IR, FINS, PFG, LH/FSH, LH, T, and TG (P<0.05) whereas it was positively correlated with ISI (P<0.05). 【Conclusion】 PCOS patients with low SHBG level are prone to imbalance of and disorders relating to sex hormones, glucose metabolism, and I- related indicators. Therefore, SHBG can be used as an important method to evaluate glucose metabolism and IR in patients with PCOS. 2017 Vol. 34 (3): 427-430 [Abstract] ( 72 ) HTML (1 KB)  PDF  (0 KB)  ( 23 ) 431 Clinical efficiency of Peripheral Blood T-SPOT.TB in Diagnosis of Tuberculosis Infections of Infants SUN Kai-sheng, LI Li-ping, YI Si-si DOI: 10.3969/j.issn.1671-7171.2017.03.005 【Objective】To assess the clinical efficiency of T-SPOT.TB in the diagnosis of tuberculosis infection within infants. 【Methods】 A total of 118 infants (less than 3 years old) with respiratory infectious diseases were enrolled in this study and underwent T-SPOT.TB, tuberculin skin test (TST) and serum tuberculosis antibody (TB-Ab IgG)detections. All the patients enrolled were from Hunan Children's Hospital from February 2013 to September 2015. The specificity and sensitivity of the three methods were compared .The diagnostic value of T-SPOT.TB applied in determining active tuberculosis was then evaluated. 【Results】According to the diagnostic criteria, 58 infants with active tuberculosis were eligible for analysis and enrolled as a tuberculosis group, and 60 infants were enrolled in non-TB group. The sensitivity and specificity of T-SPOT.TB for the diagnosis of tuberculosis infection within infants were 89.6% and 93.3% respectively, higher than those of TST (83.6% and 66.7%) and tuberculosis antibody tests(TB-Ab IgG) (65.5% and 68.3%), the difference was statistically significant (P<0.05 or P<0.01). The positive predictive value and negative predictive value of T-SPOT.TB were also higher than those of the other two methods. 【Conclusion】Peripheral blood T-SPOT.TB shows a high sensitivity and specificity for the diagnosis of tuberculosis infection within infants, which may represent a promising and faster strategy for tuberculosis diagnosis. 2017 Vol. 34 (3): 431-434 [Abstract] ( 84 ) HTML (1 KB)  PDF  (0 KB)  ( 25 ) 435 Analysis of Related Factors of Three Common Cerebrovascular Diseases ZHANG Qiong-yu, DONG Xiao-yu, NAO Jian-fei DOI: 10.3969/j.issn.1671-7171.2017.03.006 【Objective】To explore the risk factors of CSVD through the analysis of clinical characteristics of MRI markers of three common cerebral small vessel disease (CSVD) in patients, and speculate the mechanism and prediction of its development and evolution.【Methods】Patients of the three common CSVD MRI markers on the MRI with white WMH (white matter hypertensity), lacunar, perivascular spaces (perivascular space, PVS) were selected as the subjects; their general information was collected , and the fasting blood homocysteine (Hcy), serum uric acid (UA), three acyl glycerol (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) levels were analyzed. 【Results】In the CSVD group: age, smoking history, Hcy were the risk factors; in the WMH group: gender (male), age, history of diabetes, Hcy were the risk factors, HDL-C was the protective factor; in rhe lacunar group: age, smoking, LDL-C were the risk factors, and HDL-C was the protective factor. In the PVS group: age, smoking, Hcy, TG were the risk factors.【Conclusion】Advanced age, male, smoking history, Hcy, history of diabetes, high LDL-C, high TG are risk factors for CSVD. Smoking history is a high risk factor for CSVD, while HDL-C is a protective factor for CSVD. 2017 Vol. 34 (3): 435-438 [Abstract] ( 71 ) HTML (1 KB)  PDF  (0 KB)  ( 15 ) 438 Analysis of Radiofrequency Ablation in the Treatment of Locally-Advanced Pancreatic Carcinoma LI Peng, LI-Lan-bin, GUO-Hao,et al DOI: 10.3969/j.issn.1671-7171.2017.03.007 【Objective】To analysis of radiofrequency ablation in the treatment of locally-advanced pancreatic carcinoma. 【Methods】We retrospectively analyzed the clinical data of 123 patients with locally-advanced pancreatic carcinoma. All the patients were divided into a radiofrequency group and a control group according to the radiofrequency ablation surgery. And then the perioperative and prognosis data of the two groups were analyzed and compared. 【Results】In our research, all patients were successfully completed surgery and there were no cases of perioperative death. The incidence of postoperative complications of radiofrequency group was 21.7% (control group rate 10.38%) and the incidence of pancreatic fistula was 6.5% (control group rate 1.3%). There were no statistical differences between the two groups. The 6 months, 1 year and 2 years survival rates of radiofrequency group and control group were 82.4%, 43.5%, 7.6% and 55.8%, 28%, 0%, the average survival time were 13.5±0.8 months and 9.1±0.3 months. The prognosis of radiofrequency group was significantly better than that of the control group (P<0.05). 【Conclusion】Radiofrequency ablation surgery is safe and effective in the treatment of locally-advanced pancreatic carcinoma. 2017 Vol. 34 (3): 438-440 [Abstract] ( 99 ) HTML (1 KB)  PDF  (0 KB)  ( 21 ) 441 Clinical Efficacy of Docetaxel Combined with Oxaliplatin Chemotherapy Concurrent with Radiotherapy for Advanced Cervical Squamous Cell Carcinoma GAO Ya-li, ZHAO Zi-shen, GAO Ya-mei,et al DOI: 10.3969/j.issn.1671-7171.2017.03.008 【Objective】To investigate the clinical efficacy of docetaxel combined with oxaliplatin chemotherapy concurrent with radiotherapy for advanced cervical squamous-cell carcinoma. 【Methods】One hundred and twenty-five patients with locally advanced cervical squamous carcinoma in our hospital between July 2013-January 2015 were selected as the research subject. According to different therapeutic methods, patients were divided into the cisplatin group (59 cases) and Docetaxel＆Oxaliplatin group (TP group, 66 cases). The cisplatin group was given cisplatin chemotherapy concurrent with radiation therapy, while TP group was given docetaxel combined with oxaliplatin chemotherapy concurrent with radiotherapy. The curative effect and adverse reactions between the two groups were observed. 【Results】The total effective rate of TP group was 93.9% (62/66), which was significantly higher than that of the cisplatin group (81.3%, 48/59); the difference was statistically significant (P<0.05). The occurrence of gastrointestinal reaction, myelosuppression, radiation proctitis, and radiocystitis of TP group were 83.3%, 98.5%, 95.5% and 66.7%, respectively, while those in the cisplatin group were 91.5%, 96.6%, 91.5%, and 54.2%, respectively. There were no significantly statistical differences between the two groups (P>0.05). 【Conclusion】Docetaxel combined with oxaliplatin chemotherapy concurrent with radiotherapy for locally advanced cervical squamous cell carcinoma is both safe and effective. 2017 Vol. 34 (3): 441-470 [Abstract] ( 67 ) HTML (1 KB)  PDF  (0 KB)  ( 18 ) 444 Expression of miRNA-150, miRNA-146a in Serum of Children with Sepsis and its Clinical Significance YU Jie, QIAO Zhong-xiang, ZHOU Han-ming, et al DOI: 10.3969/j.issn.1671-7171.2017.03.009 【Objective】To investigate the expression of miRNA-150, miRNA-146a in serum of children with sepsis and its clinical significance.【Methods】Between February 2014 and February 2016, 62 patients with sepsis in the pediatric ICU were selected as sepsis group, and 30 children with SIRS in the same period of time were selected as SIRS group and other 30 healthy children as control group. miRNA-150, miRNA-146a, TNF- and IL-10 in peripheral blood of different levels of three groups of children with sepsis were compared. 【Results】Levels of miRNA-146a, TNF- and IL-10 in the peripheral blood of the sepsis group were significantly higher than those of the control group and the SIRS group, while the miRNA-150 level was significantly lower than that in the healthy control group and the SIRS group, the difference was statistically significant (P<0.05); levels of miRNA-146a, TNF- and IL-10 miRNA-150 in peripheral blood of different severity of sepsis in children were significantly different (P<0.05); MiRNA-150 was negatively related with the SOFA score, TNF- alpha and IL-10 (P<0.05); while MiRNA-146a was positively correlated with the SOFA score, TNF- alpha and IL-10 .【Conclusion】miRNA-150 in the peripheral blood of children with sepsis tends to be down-regulated , and the miRNA-146a tends to be up-regulated. The expression levels of the above two were closely related with levels of inflammatory factors and prognosis of the disease . 2017 Vol. 34 (3): 444-447 [Abstract] ( 85 ) HTML (1 KB)  PDF  (0 KB)  ( 28 ) 447 Value of Doppler Ultrasound in Examining Forearm Artery before Trans-Radial Coronary Intervention GUO Yan, ZHAO Zhen-xing, WU Yun-tao DOI: 10.3969/j.issn.1671-7171.2017.03.010 【Objective】 To explore the value of Doppler ultrasound in examining forearm artery before trans-radial coronary intervention (TRI). 【Methods】A total of 1358 patients undergoing elective TRI were randomly divided into ultrasound group (679 cases) and control group without ultrasound examination (679 cases). The surgical operation success rate and vascular complications were observed and compared between the two groups. 【Results】There were 129 cases of forearm artery anatomical variations in ultrasound group. The mutation rate was 19.0%. Out of these 129 cases, 120 cases underwent the left radial artery approach, while 9 patients underwent with the left radial artery approach instead. The success rate of ultrasound group was 98.7% (670/679). The control group underwent conventional right radial artery approach and the success rate was 98.5% (669/679). The postoperative arteriography of the control group showed that the incidence of forearm anatomical variation was 16.49% (112/679). There was no statistically difference between the two groups (P=0.284). The incidence of complications of the ultrasound group was 8.1% (55/679) which was lower than the control group ( 10.9%, 74/679), and the difference was statistically significant (χ2=3.092,P=0.015). 【Conclusion】Examination of forearm artery by Doppler ultrasound can not only be helpful in choosing the right artery, but also reducing vascular complication effectively. 2017 Vol. 34 (3): 447-449 [Abstract] ( 70 ) HTML (1 KB)  PDF  (0 KB)  ( 25 ) 450 Clinical Observation of Carotid Artery Peripheral Sympathetic Nerve Net Stripping Resection Combined with Bobath Rehabilitation Training in 3~6 Year-old Children with Cerebral Palsy LIU Zhi-gang, LI Hua, LI Ze,et al DOI: 10.3969/j.issn.1671-7171.2017.03.011 【Objective】To observe the clinical effect of carotid artery peripheral sympathetic nerve net stripping resection combined with Robath rehabilitation training in cerebral palsy children of 3~6 years old. 【Methods】Eighty-two cases of children with cerebral palsy were collected in our hospital from June 2010 to February 2015 as the research subjects. All children were divided into an experimental group and a control group randomly (n=41 cases each). Children in the experimental group were given carotid artery peripheral sympathetic nerve net stripping resection surgery and Bobath rehabilitation training. Children in the control group were given Bobath rehabilitation training alone. After a course of treatment, the therapeutic effect, Doppler detection of middle cerebral artery flow, and GMFM scores of the two groups were compared. 【Results】The effective rate of the experimental group was 95.12% (39/41), which was significantly higher than that of the control group (43.9%,18/41). The difference was statistically significant (χ2=5.0860.026,P＜0.026). There were no significant differences in the scores of GMFM between the two groups before treatment (P>0.05). After a course of treatment, the GMFM scores in the two groups were higher than those before treatment (P<0.05), and the GMFM scores of the experimental group scores were higher than those of the control group (P<0.05). Transcranial Doppler analysis showed that there was no significant difference in the middle cerebral artery blood flow between the two groups before treatment (P>0.05). After treatment, the mean arterial velocity, peak velocity, diastolic velocity of the middle cerebral artery in the experimental group were significantly higher than those in the control group (P<0.05). The experimental group's curative effect and the improvement of motor function were significantly higher than those of the control group. The middle artery blood flow of the experimental group also had obvious improvement after treatment. 【Conclusion】The carotid artery peripheral sympathetic nerve net stripping resection combined with Bobath rehabilitation training can obviously improve the patients' motor function as well as significantly enhance middle cerebral artery blood flow and increase clinical efficacy. 2017 Vol. 34 (3): 450-452 [Abstract] ( 71 ) HTML (1 KB)  PDF  (0 KB)  ( 22 ) 453 Effect of Intraperitoneal Bevacizumab Combined with Intraperitoneal Hyperthermic Perfusion Chemotherapy on APE1 / Ref-1 Gene Expression in Ovarian Cancer Patients LI Shao-xing DOI: 10.3969/j.issn.1671-7171.2017.03.012 【Objective】To explore the application of intraperitoneal bevacizumab (abdominal Neibeifazhu monoclonal antibody) combined with intraperitoneal hyperthermic perfusion chemotherapy in ovarian cancer patients and to detect the changes of APE1/Ref-1 gene expression before and after the treatment. 【Methods】Eighty cases of ovarian cancer were randomly divided into control group (n=40) and observation group (n=40). Two groups of patients were treated with paclitaxel and carboplatin chemotherapy. Patients in the control group received intraperitoneal hyperthermic perfusion chemotherapy treatment, and patients in the observation group received intraperitoneal hyperthermic perfusion chemotherapy combined with intraperitoneal bevacizumab (Neibeifazhu monoclonal antibody). The clinical efficacy and APE1/Ref-1 gene expression were compared between the two groups.【Results】After 4 weeks of treatment, the curative effect of the observation group was 90%, which was higher than that of the control group (67.50%), and the difference was significant (P<0.05). The adverse reaction rate had no significant difference between the observation group (6/40, 15%) and control group(7/40,17.5%) (P>0.05). Real-time PCR showed that APE1/Ref-1 gene expression was significantly increased after treatment in the two groups compared to that before treatment. The changes of APE-1/Ref-1 expression level in the observation group was significantly higher than that in the control group (P<0.05). 【Conclusion】Compared to intraperitoneal hyperthermic perfusion alone, the clinical effect of bevacizumab combined with intraperitoneal hyperthermic perfusion chemotherapy in ovarian cancer patients is better. During this combo treatment, APE1/Ref-1 gene expression was significantly enhanced in ovarian cancer tissues to improve the sensitivity to chemotherapy. It is worthy of popularization and application. 2017 Vol. 34 (3): 453-456,458 [Abstract] ( 72 ) HTML (1 KB)  PDF  (0 KB)  ( 22 ) 456 Correlation between Ultrasound Imaging Features and Sex Hormones/ Insulin Resistance Index in Polycystic Ovary Syndrome ZHANG Jie, ZHANG Xiang, YAN Jing,et al DOI: 10.3969/j.issn.1671-7171.2017.03.013 【Objective】To investigate the correlation between ultrasonographic features and sex hormones and insulin resistance index in patients of polycystic ovary syndrome (PCOS). 【Methods】 Ninety patients with PCOS from February 2014 to May 2016 in our hospital were selected as observation group, and 53 cases in the same period for health examination were selected as control group. Ultrasonic imaging detection was performed in the two groups of patients, the detecting indexes included ovarian total area (TA), ovarian stromal area (SA);At the same time the ratio of SA/TA was calculated, sex hormone levels, blood glucose levels and other related indicators were detected in the two groups of patients , and correlation between the sonographic characteristics and sex hormone index and the HOMA-IR of patients in the observation group was analyzed. 【Results】 Compared with the control group, the luteinizing hormone (LH), testosterone (T), estradiol (E2) and fasting blood glucose (FBG) and HOMA-IR of the observation group of patients increased significantly, the differences were statistically significant (P<0.05);compared with the control group, TA, SA and SA/TA of the observation group of patients increased significantly, the difference was statistically significant (P<0.05);Person correlation analysis showed that SA of the observation was positively correlated with LH, T, E2 significantly(r=0.280,0.610,0.389, P<0.05);SA/TA, and LH, LH, T,E2 also showed a significant positive correlation (r=0.265,0.378,0.332, P<0.05);there were significant positive correlation between TA and E2 (r=0.315, P<0.05).SA/TA ratio was positively correlated with HOMA-IR (r=0.712, P<0.05). 【Conclusion】 Ovarian SA and TA of patients with PCOS are significantly higher than those of healthy physical examination, and the ultrasonic image characteristics are significantly correlated with sex hormones and HOMA-IR. 2017 Vol. 34 (3): 456-458 [Abstract] ( 82 ) HTML (1 KB)  PDF  (0 KB)  ( 21 ) 459 Effect of Ginsenoside Rg3 Combined with Neoadjuvant Chemoradiotherapy on the Expression of VEGF and MTA1 Protein in Gastric Cancer Tissues and Prognosis LI Xiang-wei, ZHANG Zheng-yuan, ZHOU Guang-rong DOI: 10.3969/j.issn.1671-7171.2017.03.014 【Objective】To study the effect of ginsenoside Rg3 combined with neoadjuvant chemoradiotherapy on the expression of VEGF and MTA1 protein in gastric cancer tissues, immune function and prognosis. 【Methods】A total of 84 patients with gastric cancer were randomized into the control group and the treatment group according to different methods of treatment. The control group was given neoadjuvant concurrent radiotherapy and chemotherapy before operation, while the experimental group was treated with ginsenoside Rg3 in addition to concurrent radiotherapy and chemotherapy. The changes of expression of VEGF and MTA1 protein, immune function and prognosis were compared between the two groups before and after treatment. 【Results】The RO resection rate and the total effective rate of the treatment group were higher than those of the control group (P<0.05). Compared to pretreatment, the positive rates of VEGF and MTA1 post-treatment in both groups were significantly reduced (P<0.05), and the decrease in the experimental group was significantly greater than that in the control group (P<0.05). After treatment, the percentage of CD3+ and CD4+, CD4+/CD8+ value and conversion rate of T cells in the experimental group increased significantly compared to the control group (P<0.05), while the percentage of CD8+ decreased significantly (P < 0.05). There was no significant difference in the incidence of bone marrow suppression between the two groups (P>0.05). The recurrence rate in the experimental group was low than the control group (P<0.05). The 1-year, 3-year and 5-year survival rates of the experimental group were higher than those of the control group (P<0.05). 【Conclusion】Ginsenoside Rg3 combined with neoadjuvant chemoradiotherapy in patients with gastric cancer shows higher RO resection rate. It can significantly lower the expression of VEGF and MTA1, improve body immunity, reduce recurrence rate, and increase the survival rate of patients. The effect is significant in clinical application. 2017 Vol. 34 (3): 459-461 [Abstract] ( 44 ) HTML (1 KB)  PDF  (0 KB)  ( 25 ) 462 Changes of Pregnancy-related Anxiety and Its Influencing Factors in 4116 cases of Pregnant Women in Zigong City ZENG Liu-ping, LI Jun, ZENG Ru-jue DOI: 10.3969/j.issn.1671-7171.2017.03.015 【Objective】 To investigate changes of pregnancy-related anxiety and its influencing factors in 4116 cases of pregnant women in Zigong City. 【Methods】A total of 4116 cases of pregnant women who possessed obstetric cards with regular inspection in our hospital were recruited for this research. Subjects were divided into an anxiety group and a non-anxiety group according to maternal anxiety scoring. We analyzed the general information, clinical data and anxiety-influencing factors in the two groups of pregnant women to determine the independent influencing factors. 【Results】There were 603 anxiety cases in a total of 4116 pregnant women, and the incidence rate was 14.7%. Age distribution, family income, smoking and drinking history, marital status, history of abortion, planned pregnancy, fetal gender expectation, determine the mode of delivery, worries of inherited diseases and health status of the fetus were influencing factors of anxiety in pregnant women determined by single factor analysis. Multiple-factor analysis showed that smoking and drinking history, marital status, history of abortion, planned pregnancy, fetal gender expectation, worries of inherited diseases and health status of the fetus were the independent influencing factors of anxiety in pregnant women. 【Conclusion】Smoking and drinking history, marital status, history of abortion, planned pregnancy, fetal gender expectation and worries of inherited diseases and health status of the fetus were the independent influencing factors of anxiety in pregnant women. Pregnant women with different anxiety-influencing factors should be given different psychological intervention to effectively reduce the incidence of anxiety during pregnancy. 2017 Vol. 34 (3): 462-465 [Abstract] ( 59 ) HTML (1 KB)  PDF  (0 KB)  ( 27 ) 465 Application of Dezocine and Sufentanil Combined with Different Doses of Dexmedetomidine during Surgery in Abdominal Hysterectomy WANG Dan DOI: 10.3969/j.issn.1671-7171.2017.03.016 【Objective】To investigate the application of dezocine and sufentanil combined with different doses of dexmedetomidine in abdominal hysterectomy surgery. 【Methods】A total of 160 cases after the abdomen womb excision surgery were divided into four groups, with 40 cases in each group. Group A, group B and group C were given dezocine and sufentanil combined with 0.5 μg/kg, 1.0 μg/kg and 1.5 μg/kg dexmedetomidine ,respectively,and group D was only given dezocine and sufentanil. Degree of pain (VAS), degree of comfort (BCS), Ramsay sedation score and incidence of adverse reactions were compared postoperative 1h (T1), 6h (T2), 12h (T3), 24h (T4), 48h (T5) amongst the four groups of patient-controlled intravenous analgesia (PCIA). 【Results】Compared with group A and D, the VAS scores at T2, T3 of group B and C were significantly decreased (P<0.05) and the BCS score at T3, T4, T5 were dramatically increased (P<0.05). Compared to g T3, T4, T5 moment BCS scores were significantly increased (P<0.05). Compared to group C, the Ramsay scores of group B at T3 and T4 were significantly decreased (P<0.05). The incidence of adverse reactions in group A and group B were lower than those of group C and D (P<0.05). 【Conclusion】 In abdominal hysterectomy, dezocine and sufentanil combined with 1.0 μg/kg dexmedetomidine postoperative analgesia has better effect, fewer adverse reactions, and more comfort; it is worth clinical attention. 2017 Vol. 34 (3): 465-467 [Abstract] ( 77 ) HTML (1 KB)  PDF  (0 KB)  ( 24 ) 468 Effect of Retroperitoneal Laparoscopic Radical Nephrectomy on Hepatic, Renal Function and Myocardial Enzymes in Perioperative Patients MAO Fei, GU Shuo, ZHONG Bin,et al DOI: 10.3969/j.issn.1671-7171.2017.03.017 【Objective】To investigate the effect of retroperitoneal laparoscopic radical nephrectomy on liver,renal function and myocardial enzymes in patients with in perioperative period. 【Methods】A total of 80 patients with renal carcinoma who received surgical treatment in our hospital were analyzed.Forty two cases undergoing retroperitoneal laparoscopic radical nephrectomy were enrolled in the observation group, and 38 cases undergoing open radical nephrectomy were enrolled in the control group. The changes of liver function (ALT,AST), renal function (BUN,Cr ), myocardial enzymes (CK,CK-MB,LDH) and inflammatory factors (CRP,TNF-α, IL-6 ) were compared between the two groups. The operation complications in the two groups were recorded. 【Results】The intraoperative blood loss, hospitalization time and incidence of complications in the observation group were significantly less and lower than those in the control group (P<0.05). There were no significant level changes of ALT, AST, BUN, Cr, CK, CK-MB and LDH in observation group between pre and 24h post operation (P>0.05). While levels of ALT, AST and LDH at 24h post-operation in the control group were increased significantly (P<0.05). At 24h post-operation, the differences in ALT, AST and LDH between the two groups were significant (P<0.05). The levels of TNF-α, IL-6 and CRP at 24h post-operation in the two groups were significantly higher than those before operation (P<0.05), however, the above indexes in observation group were significantly lower than those in the control group (P<0.05). 【Conclusion】Retroperitoneal laparoscopic radical nephrectomy has the advantages of minimal trauma, faster postoperative recovery and few complications. It has little effect on hepatic and renal function as well as myocardial enzymes. Meanwhile, it has less influence on inflammatory factors, if compared to open operation. 2017 Vol. 34 (3): 468-470 [Abstract] ( 75 ) HTML (1 KB)  PDF  (0 KB)  ( 15 ) 471 Ultrasonographic Study of Renal Hemodynamics in Neonates with Mild Asphyxia and Meconium Aspiration Syndrome HU Wen-wen, CHEN Hong-tian, XIA Xiao-hui, et al DOI: 10.3969/j.issn.1671-7171.2017.03.018 【Objective】To investigate the ultrasonographic characteristics of renal hemodynamics in mild asphyxia neonates with meconium aspiration syndrome (MAS). 【Methods】Thirty cases of full-term newborns with mild asphyxia from July 2015 to July 2016 in our hospital were collected for the research. According to whether or not they had meconium aspiration syndrome (MAS), the patients were divided into two groups: group A (15 cases without MAS) and Group B (15 cases with MAS). At the same time, 16 healthy full-term newborn infants were selected as group C (the control group). The hemodynamic parameters of the bilateral main renal artery were detected by color Doppler while the baby was sleeping quietly. Systolic peak systolic velocity (PSV), end - diastolic velocity (EDV) and resistance index (RI) were measured on d1, d3 and d7 after birth. 【Results】The PSV and EDV values of group A and B on d1 were lower than those of group C (P<0.05), while RI values were higher than that of group C (P<0.05). The EDV of group B on d1 was lower than that of group A, and the RI was higher than that of group A (P<0.05). Both PSV and EDV of group B on d3 were lower than those of group A and group C; the difference was statistically significant (P<0.05). There were no significant differences in PSV,EDV and RI on d7 between group A and group B (P>0.05). The PSV and EDV of group A were the lowest and the RI was the highest on d1 after birth, after which the PSV and EDV increased gradually while the RI decreased gradually on d3 and d7. Finally the differences of PSV,EDV and RI of group A between d1 and d7 were statistically significant (P<0.05). The PSV and EDV of group B were both low and RI was the highest on d1, then the PSV on d3 was further reduced while the EDV was slightly increased and RI became the lowest on d3. On d7, all of PSV, EDV and RI in group B were increased; PSV increased the most. The differences of PSV,EDV and RI of group B between d1 and d7 were statistically significant (P<0.05). In group C, PSV and EDV showed a tendency to increase gradually, while RI showed a decreasing tendency, but the different trends among them were not statistically significant(P＞0.05). 【Conclusion】In neonates with mild asphyxia, the renal blood supply is reduced. it is more obvious in diastolic (EDV). When accompanied with MAS, the renal blood flow decreases even more significantly, and it will last longer and take slower recovery. Our research suggested that color Doppler ultrasonography can detect the change of renal blood supply to provide useful information for clinical treatment. 2017 Vol. 34 (3): 471-474 [Abstract] ( 78 ) HTML (1 KB)  PDF  (0 KB)  ( 28 ) 475 Application of Dexmedetomidine and Midazolam Combined with Sufentanil in Patients with Visible laryngoscopy Conscious Tracheal Intubation XU Hui-juan, PEN Qing-xiong, PAN Dao-bo,et al DOI: 10.3969/j.issn.1671-7171.2017.03.019 【Objective】To investigate the efficacy and safety of dextromethorphan and midazolam combined with sufentanil in patients with difficult airway using visible laryngoscopy (glidescope) conscious tracheal intubation.【Methods】Forty patients with difficult airway (Mallampati grade >Ⅲ) from the First Hospital of Changsha were randomly divided into two groups: the dexmedetomidine and midazolam combined with sufentanil (group DM), and the midazolam combined with sufentanil group (group M), with 20 cases in each group. At 15min before tracheal intubation, group DM was treated with dexmedetomidine (1μg/kg) and midazolam intravenous infusion (0.01mg/kg), while group M received intravenous infusion of midazolam 0.02mg/kg. At 5min before intubation group DM and group M were given sufentanil (0.1μg/kg, dilute with saline at 1ug/ml, intravenous drip). After the infusion, patients of two groups underwent thyrocricoid puncture injection of 2% lidocaine (3ml). All patients were selected to be intubation through mouth by gliscope (visible laryngoscopy). Heart rate (HR), systolic blood pressure (SBP),and diastolic blood pressure (DBP) were recorded at entering OR 10min (T0), before thyrocricocentesis (T1), post-thyrocricocentesis (T2), video laryngoscope (T3), and tracheal catheter insert the glottis (T4). Ramsay sedation score of the two groups of patients at T0, T1 and T2 were recorded as well. Lethargy, respiratory depression, hypoxia and cough (choking) in the process of intubation were recorded as well as postoperative hoarseness and incidence of sore throat. 【Results】At T4, the HR, SBP and DBP in group DM were significantly lower than those in group M (P<0.05). In both group DM and group M, the values of HR, SBP and DBP at T1 were significantly lower than those at T0 (P<0.05). The values of HR, SBP and DBP at T4 in group M was significantly higher than those at T0. There was no significant difference in sedation score between the two groups in the T0. The sedation scores at T1 and T2 in group M were higher than those in group DM (P<0.05). In both group DM and group M, the sedation scores at T1 and T2 were significantly higher than those at T0 (P<0.05). The incidence of drowsiness (lethargy) and cough (choking) of group M were significantly higher than those of group DM (P<0.05). There was no significant difference in the incidence of respiratory depression and sore throat between the two groups (P> 0.05). There was no hoarseness in both groups.【Conclusion】Compared to midazolam combined with sufentanil, dexmedetomidine and midazolam combined with sufentanil for patients with difficult airway undergoing visible laryngoscopy (glidescope) conscious tracheal intubation has more advantages. It can keep blood pressure and heart rate more stable and lowering incidence rate of drowsiness, cough (choking), and other adverse reactions. 2017 Vol. 34 (3): 475-478,480 [Abstract] ( 76 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 478 Effects of Different Anesthesia Methods on Postoperative Agitation and Hemodynamics in Postoperative Recovery Period of Children with Tonsillectomy SU Yi-feng DOI: 10.3969/j.issn.1671-7171.2017.03.020 【Objective】To explore the effects of different anesthesia methods on postoperative restlessness and hemodynamic changes in children during recovery period after tonsillectomy. 【Methods】A total of 120 children with tonsillectomy in our hospital were divided into group A, B and C (40 cases in each) according to different anesthesia. Children in group A received sevoflurane with a low dose of dexmedetomidine; group B with sevoflurane plus a single ketamine; group C received sevoflurane and saline. Changes of hemodynamics before and after surgery, and postoperative restlessness were observed and compared among the three groups. 【Results】There were no significant differences in mean arterial pressure (MAP), heart rate (HR) and blood oxygen saturation (SpO2) among the three groups before and after anesthesia induction (P>0.05). The MAP of group A was higher than that of group C at the beginning of operation and at the end of operation (P<0.05). However, there was no significant difference between group A and group B (P>0.05). The HR and SpO2 of group A were lower than those of group B and group C at the beginning of operation and at the end of operation (P<0.05). The Ramsay sedation score of group A was significantly higher than that of group B and group C (P<0.05). The extubation and discharge time in group A were shorter than those in group B and group C. significant difference (P<0.05). 【Conclusion】Dexmedetomidine can effectively reduce postoperative agitation in children after tonsillectomy recovery period, stabilize hemodynamics and reduce hemodynamic fluctuations during operation. It has good sedation and analgesic effect, it is profound clinical application. 2017 Vol. 34 (3): 478-480 [Abstract] ( 41 ) HTML (1 KB)  PDF  (0 KB)  ( 22 ) 481 Correlation between Cytokine Levels in Amniotic Fluid of Second Trimester with Spontaneous Preterm Delivery in Middle-aged Pregnant Women LIU Jing DOI: 10.3969/j.issn.1671-7171.2017.03.021 【Objective】To study the correlation between cytokine levels in amniotic fluid during the second trimester and spontaneous preterm delivery in middle-aged women. 【Methods】Forty-three pregnant women with spontaneous preterm delivery who were admitted to our hospital from March 2014 to November 2015 were selected as the premature group. Forty normal pregnant women were selected as the control group. The levels of cytokines (IL-16, IL-8, IL-6, CRP, and TNF-α ) were measured from amniotic fluid of the two groups, and the correlation of cytokines' level with spontaneous preterm delivery was analyzed. 【Results】The levels of IL-16, IL-8, IL-6, CRP and TNF-α in premature group were (201.37±18.74)pg/mL、(786.14±146.28)pg/mL,(141.25±13.87)pg/mL,(176.22±12.34)ng/mL, and(114.27±15.64)pg/mL, respectively, significantly higher than those in the control group[(157.46±10.43)pg/mL,(413.91±84.67)pg/mL,(122.94±8.63)pg/mL,(132.18±8.64)ng/mL, and(76.48±7.69)pg/mL], respectively. The differences were statistically significant (P<0.05). Through the logistic analysis of influencing factors, the levels of IL-16, IL-8, IL-6, CRP and TNF-α were found to be significantly correlated with the occurrence of spontaneous preterm birth in middle-aged women (P<0.05). 【Conclusion】 The level of cytokines in amniotic fluid of middle pregnancy is closely related to the occurrence of spontaneous preterm delivery in middle-aged pregnant women, and it can be used as early clinical screening indicators. 2017 Vol. 34 (3): 481-483 [Abstract] ( 32 ) HTML (1 KB)  PDF  (0 KB)  ( 24 ) 484 Application of Preoperative Three-dimensional CT Simulated Puncture in Percutaneous Nephrolithotomy LIU Lin, ZHU Hai-tao, MIAO Long,et al DOI: 10.3969/j.issn.1671-7171.2017.03.022 【Objective】To study the value of preoperative three-dimensional (3D) CT reconstruction simulated puncture for percutaneous nephrolithotomy. 【Methods】A total of 100 cases of patients with upper urinary stones who received percutaneous nephrolithotomy surgery in our hospital were collected. Patients were divided according to whether or not they received three-dimensional CT reconstruction simulation into the control group (without 3D CT simulation) and the observation group (with 3D CT simulation). Clinical data (the puncture success rate, the stone clearance rate, and complications) were compared between the two groups. 【Results】There were no statistical significant differences in hydronephrosis and stone location in patients of the two groups (P>0.05). In the observation group, percutaneous puncture time, channel number, postoperative hemoglobin value drop and colon injury were significantly lower than those in the control group (P<0.05), while the stone clearance rate and one-time puncture success rate were significantly higher than those in the control group, with statistical significance (P<0.05). 【Conclusion】Percutaneous renal surgery under the guidance of 3D CT reconstruction of simulated puncture can effectively interpret the morphology of stones and kidney collection system. This method can improve the accuracy of preoperative evaluation and provide the most effective method of puncture, increasing puncture success rate and stone clearance probability with minimal unnecessary damage to surrounding tissue. 2017 Vol. 34 (3): 484-486 [Abstract] ( 60 ) HTML (1 KB)  PDF  (0 KB)  ( 27 ) 486 Pathogenic Analysis of Chronic Suppurative Otitis Media and the Influencing Factors of Non-dry Ear after Surgery WANG Fang, HU Xiao-yan, JIANG Liang DOI: 10.3969/j.issn.1671-7171.2017.03.023 【Objective】To analyze the pathogen characteristics of chronic suppurative otitis media and postoperative factors affecting of occurance of non-dry ear in order to improve the cure rate of chronic suppurative otitis media and provide a basis to reduce the complications.【Methods】A total of 243 patients who were treated with surgery for chronic suppurative otitis media in our hospital from Jan 2013 to Jan 2015 were included in this study. Etiology monitoring was conducted on the middle ear cavity discharge , and analysis was carried out on pathogenic bacterial distribution and drug sensitivity;The patients were divided into dry ear group and non-dry ear group; statistical analysis was made on age, gender, smoking, alcohol abuse, basic disease, nutritional status, clinical data and laboratory results; the postoperative risk factors of non-dry ear were evaluated.【Results】 Pathogenic bacteria was detected in 213 of 255 ear pus samples, the detection rate was 83.5%; A total of 268 strains of pathogens were detected , including 154 Gram- positive bacteria which accounted for 57.5% of total pathogens, and 92 Gram- negative ones which accounted for 34.3%; and 22 fungi ones accounting for 8.2%. Drug sensitive tests showed that Gram- positive bacteria was sensitive to glycopeptide antibiotics , and the Gram-negetive was sensitive to polymyxin B and carbapenems; Fungi was sensitive to antifungal drug. Non-dry ear after surgery was associated with smoking, diabetes and fungal infections (especially mucormycosis related), the difference of the two groups was statistically significant (P<0.05).【Conclusion】The treatment of chronic suppurative otitis media should be based on sensitive antibiotic. Attention should be paid to the possibility of fungal infection, and patients are strongly advised to stop smoking and strictly control blood sugar. 2017 Vol. 34 (3): 486-489 [Abstract] ( 67 ) HTML (1 KB)  PDF  (0 KB)  ( 22 ) 490 Effect of Sivelestat on the Expression of Inflammatory Factors, ALT and AST in Rats with Severe Acute Pancreatitis TAO Jia-li, LUO Man DOI: 10.3969/j.issn.1671-7171.2017.03.024 【Objective】To study the effect of sivelestat on the expression of inflammatory factors, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in rats with severe acute pancreatitis. 【Methods】A total of 30 SD rats were divided into control group, model group and intervention group. Rats in the control group underwent a ventral midline incision to exposure pancreas and duodenum without clamping. Severe acute pancreatitis (SAP) was established in the model group and the intervention group by retrograde injection of 5% sodium taurocholate from the duodenal papilla into the biliopancreatic duct. The control group and model group were injected with saline solution by jugular vein catheter, and the intervention group received the sivelestat treatment. The levels of serum inflammatory factors (IL-2, IL-6 and IL-8), ALT and AST were measured at 6h and 12h after SAP initiation. At the same time points, the pathological score of pancreatic tissue was evaluated. 【Results】At 6h and 12h after SAP initiating, the pathological score of pancreatic tissue and serum levels of amylase (AMY), ALT, AST, IL-2, IL-6 and IL-8 in the model group rats were significantly higher than those in the control group(t=10.129~21.664, P<0.01). However, the pathological score of pancreatic tissue and serum levels of AMY, ALT, AST, IL-2, IL-6 and IL-8 in the intervention group were significantly lower than those in the model group (t=6.055~12.551,P<0.01). 【Conclusion】Sivelestat can alleviate pancreatic damage of severe acute pancreatitis in rat, inhibit inflammation, and protect liver function. 2017 Vol. 34 (3): 490-493,495 [Abstract] ( 72 ) HTML (1 KB)  PDF  (0 KB)  ( 14 ) 493 A Controlled Study of Hemodialysis Combined with Hemoperfusion for the Treatment of Microinflammation in Diabetic Nephropathy GOU Yan DOI: 10.3969/j.issn.1671-7171.2017.03.025 【Objective】The aim of this study was to assess the effect of hemodialysis combined with hemoperfusion for the treatment of microinflammation in diabetic nephropathy patients. 【Methods】A total of 125 patients with diabetic nephropathy (DN) were divided into two group randomly. The observation group (n=66) received hemodialysis combined with hemoperfusion, and the control group (n=59) received hemodialysis therapy only. The status of curative effect and microinflammation which reflected as serum amyloid A(SAA),interleukin-6 (IL-6),IL-1β,tumor necrosis factor (TNF-α) and transforming growth factor (TGF-β1) were compared. 【Results】The effective rate of observation group was 60.61%, which was significantly higher than that in control group (45.760%) (P<0.05). After treatment,the levels of SAA, IL-6, IL-1β, TNF-α and TGF-β1 were significantly lower in the observation group than those in the control group. The differences were statistically significant (P<0.01). 【Conclusion】Hemodialysis combined with hemoperfusion can effectively decreased microinflammation status in diabetic nephropathy. 2017 Vol. 34 (3): 493-495 [Abstract] ( 76 ) HTML (1 KB)  PDF  (0 KB)  ( 22 ) 496 Expression and Significance of Gonadal Hormone Levels in Infertility Patients LI Hai-yan, YU Ling-hui, CAO Deng-cheng DOI: 10.3969/j.issn.1671-7171.2017.03.026 【Objective】 To investigate the clinical value of the detection of serum donadal hormone levels in the diagnosis and treatment of infertility. 【Methods】A total of 48 cases of infertility patients from August 2013 to December 2015 in our hospital constituted the observation group. At the same time, 30 cases of healthy women were selected as the healthy control group. The expression levels of serum sex hormone related factors such as luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone (T), progesterone (P), estradiol (E2), Prolactin (PRL) and related antibodies such as endometrial antibody (EMAb), antisperm antibody (AsAb), anti zona pellucida antibodies (AzpAb), anti-ovarian Antibody (antiovary antibody, AoAb) were observed and compared between the two groups. 【Results】Compared to the control group, the T and PRL levels of the observation group were significantly increased, P and E2 levels were significantly reduced (P<0.05); while EMAb, AsAb, AzpAb and AoAb positive expression rate was significantly higher (P<0.05). 【Conclusion】 Sex hormone levels and positive expression of related antibodies have important clinical significance in the diagnosis and treatment of infertility patients. 2017 Vol. 34 (3): 496-498,501 [Abstract] ( 83 ) HTML (1 KB)  PDF  (0 KB)  ( 23 ) 498 Relationship of NINJ2 Gene Polymorphism with Ischemic Stroke in Xi'an Area KONG Xiang-li, ZHANG Lei, LI Jun-chao DOI: 10.3969/j.issn.1671-7171.2017.03.027 【Objective】To investigate the relationship between the nerve injury induced protein 2 gene (NINJ2) polymorphism and ischemic stroke in Xi'an area. 【Methods】From May 2014 to April 2016, 218 cases of ischemic stroke patients in our hospital were selected as observation group, at the same time, 124 healthy persons were selected as the control group. Polymerase chain reaction-restriction fragment length polymorphism was used to detect the genotype in locus of rs10849373, rs12425791, rs11833579 and rs11063806 in gene NINJ2 , the differences of genotype and allele frequencies between the two groups were compared. 【Results】 Frequency distribution of GG, GA and AA genotype in rs10849373, rs12425791, rs11833579 and rs11063806 locus accords with the Hardy- Weinberg equilibrium in the observation group; the frequency of rs10849373 locus AA , GA genotype and allele A of the observation group were 11.47%, 41.28% and 32.11%, significantly higher than those of the control group; distribution difference of rs12425791, 11833579 and rs11063806 locus between the observation group and the control group was not statistically significant (P＞0.05). 【Conclusion】 rs10849373 locus of gene NINJ2 is associated with ischemic stroke in Xi'an area.The exact mechanism needs further researches. 2017 Vol. 34 (3): 498-501 [Abstract] ( 94 ) HTML (1 KB)  PDF  (0 KB)  ( 24 ) 502 Blood pressure variability in patients with coronary heart disease and hypertension and ambulatory blood pressure ZHANG Yong-qiang, CHI Qing-bing DOI: 10.3969/j.issn.1671-7171.2017.03.028 【Objective】To analyze the changes of blood pressure variability and ambulatory blood pressure in patients with hypertension and coronary heart disease.【Methods】Clinical data of 160 patients with hypertension in our hospital from June 2014 to June 2015 were selected.According to the results of coronary angiography (CGA) or coronary CT imaging (CTA), the patients were divided into coronary heart disease with hypertension (n=80, the observation group) and single hypertension group (n=80, the control group). The dynamic blood pressure and blood pressure variability were compared between the two groups.【Results】In the observation group, daytime average systolic blood pressure, nighttime average systolic blood pressure, 24h mean systolic blood pressure, daytime average pulse pressure, nighttime average pulse pressure, 24h average pulse pressure, daytime average systolic blood pressure standard deviation, average nighttime systolic blood pressure standard deviation, 24h average systolic blood pressure standard deviation were significantly higher than those in the control group, the difference was statistically significant (all P<0.05); in the comparison of the daytime average diastolic blood pressure, nighttime average diastolic blood pressure, 24h mean diastolic blood pressure, daytime average diastolic blood pressure standard deviation, average nighttime diastolic blood pressure standard deviation and 24h mean diastolic blood pressure standard deviation , there were no significant differences in the two groups(P>0.05).【Conclusion】Patients with hypertension complicated with coronary heart disease have more dynamic blood pressure fluctuation,and the blood pressure variability increases correspondingly.To strengthen the monitoring of ambulatory blood pressure and blood pressure variability has a certain role in the diagnosis and treatment of coronary heart disease with hypertension. 2017 Vol. 34 (3): 502-504 [Abstract] ( 105 ) HTML (1 KB)  PDF  (0 KB)  ( 17 )

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