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| Effect of Sivelestat on the Expression of Inflammatory Factors, ALT and AST in Rats with Severe Acute Pancreatitis |
| TAO Jia-li, LUO Man |
| Emergency Department,Huai'an First People's Hospital affiliated to Nanjing Medical University,Huai'an,Jiangsu 223300,China |
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Abstract 【Objective】To study the effect of sivelestat on the expression of inflammatory factors, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in rats with severe acute pancreatitis. 【Methods】A total of 30 SD rats were divided into control group, model group and intervention group. Rats in the control group underwent a ventral midline incision to exposure pancreas and duodenum without clamping. Severe acute pancreatitis (SAP) was established in the model group and the intervention group by retrograde injection of 5% sodium taurocholate from the duodenal papilla into the biliopancreatic duct. The control group and model group were injected with saline solution by jugular vein catheter, and the intervention group received the sivelestat treatment. The levels of serum inflammatory factors (IL-2, IL-6 and IL-8), ALT and AST were measured at 6h and 12h after SAP initiation. At the same time points, the pathological score of pancreatic tissue was evaluated. 【Results】At 6h and 12h after SAP initiating, the pathological score of pancreatic tissue and serum levels of amylase (AMY), ALT, AST, IL-2, IL-6 and IL-8 in the model group rats were significantly higher than those in the control group(t=10.129~21.664, P<0.01). However, the pathological score of pancreatic tissue and serum levels of AMY, ALT, AST, IL-2, IL-6 and IL-8 in the intervention group were significantly lower than those in the model group (t=6.055~12.551,P<0.01). 【Conclusion】Sivelestat can alleviate pancreatic damage of severe acute pancreatitis in rat, inhibit inflammation, and protect liver function.
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Received: 14 January 2017
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2017, Vol. 34 
