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Quick Search     Adv Search 2021 Vol. 38, No. 10 Published: 2021-10-26 Review and lecture Original Article          Original Article 1448 Mechanism of LncRNA HOTAIR in Photodynamic Therapy of Colorectal Cancer GONG Hui, LIAO Ping, YANG Qing-long, et al DOI: 10.3969/j.issn.1671-7171.2021.10.003 【Objective】To explore the mechanism of long non-coding RNA(LncRNA)-HOX transcribed antisense RNA(HOTAIR) in photodynamic therapy (PDT) of colorectal cancer. 【Methods】The expression of lncrna hotair in colorectal cancer cells (CRCs) treated with PDT was detected by real-time fluorescence quantitative PCR (qPCR). CCK8 kit and plate clone formation experiment were used to detect the proliferation of CRCs after interfering with the expression of LncRNA HOTAIR and treated with PDT. Meanwhile, western blot assay (WB) was used to detect the expression of cell proliferation-related proteins p38 and p-p38.Then, the intersection of LncRNA HOTAIR downstream regulatory protein and PDT sequencing data was analyzed by Starbase database to obtain both the regulatory protein of LncRNA HOTAIR and the down-regulated protein after PDT treatment. Finally, after interfering with the expression of LncRNA HOTAIR and overexpressing ENPP5, the cell proliferation level was detected by CCK8 kit and plate cloning experiment, and the expression of p38 and P-38 protein were detected by WB experiment.【Results】After PDT treatment, the cell morphology of CRCs changed significantly, and the expression of LncRNA HOTAIR was significantly down regulated. Interference with LncRNA HOTAIR expression in CRCs can improve the sensitivity to PDT. LncRNA HOTAIR in CRCs can positively regulate ENPP5 expression. Interfering with the expression of LncRNA HOTAIR in CRCs can down regulate the expression of ENPP5, affect the p38 signal pathway and promote the sensitivity to PDT.【Conclusion】Interference with LncRNA HOTAIR expression increased CRCs sensitivity to PDT, and LncRNA HOTAIR positively regulated ENPP5 to affect PDT sensitivity to CRCs. It provides a new idea for improving the efficacy of PDT in colorectal cancer. 2021 Vol. 38 (10): 1448-1452 [Abstract] ( 42 ) HTML (1 KB)  PDF (2247 KB)  ( 55 ) 1453 Analysis of Medical Malpractice in Certain Area of Shanghai during 2014-2018 to Provide Possible Regulatory Strategies SUN Chang DOI: 10.3969/j.issn.1671-7171.2021.10.004 【Objective】To provide suggestions for the prevention and treatment of medical injury through analyzing the causes of medical injury liability cases of 2014-2018.【Methods】Retrospective analysis of 446 cases for medical injury disputes in certain area of Shanghai from 2014 to 2018 was performed by expert judgement and statistical analysis methods to find out loopholes in medical quality and safety management and provide detailed suggestions for medical malpractice prevention. 【Results】Medical damage incidence were higher in tertiary hospitals. Surgical departments had more medical injury cases than non-surgical departments. There were 5 high-risk medical injury departments, such as emergency department and orthopedics department. There were 5 causes for high-risk medical injury such as inadequate information and lax observation of medical condition. 【Conclusion】Health administrative departments and medical institutions should strengthen the quality management of medical records, strengthen trainings of medical humanistic care and communication. ,and strengthen the supervision and inspection of medical quality and safety incidents. 2021 Vol. 38 (10): 1453-1456 [Abstract] ( 40 ) HTML (1 KB)  PDF (1168 KB)  ( 59 ) 1457 Effect of Perioperative Group Counseling on Improving Shoulder Function after Arthroscopic Rotator Cuff Surgery WANG Hong-Tao, WANG Jing, JIANG Hao, et al DOI: 10.3969/j.issn.1671-7171.2021.10.005 【Objective】To explore the clinical effect of perioperative group counseling for patients who had shoulder arthroscopic rotator cuff repair. To provide the theory basis for the application of perioperative group counseling mode and lay the theoretical foundation for clinical promotion. 【Methods】A total of 180 patients who had the shoulder arthroscopic rotator cuff repair in Hunan Provincial People's Hospital from August 2017 to May 2018 were randomly divided into two groups which were the routine rehabilitation group (the control group) and the counseling group (the observation group). And each group had 90 patients. The pain score (VAS), range of motion of shoulder, American Shoulder and Elbow Surgical Scoring Scale (ASES), UCLA shoulder Scoring System (UCLA) and other indicators were compared before the operation, 1 month, 3 months and 6 months after surgery. 【Results】 (1) There were no significant differences in preoperative VAS score, UCLA score and ASES score between 2 groups (P>0.05); (2) At 1, 3 and 6 months after surgery, the VAS score, UCLA score, ASES score and range of motion of shoulder in the observation group were better than those in the control group (P<0.05); (3) The difference and absolute value of difference between pre-operation and 6 months after surgery showed that the VAS score, UCLA score, ASES score, range of motion of shoulder joint and practice rate of patients in the observation group were better than those in the control group (P<0.05). 【Conclusion】 (1) Pain relief, treatment satisfaction, shoulder function and range of motion are improved in both groups. (2) Compared to the conventional mode, perioperative group counseling mode has better effects on postoperative patients. (3) Intervention mode and intervention time are the main factors to improve pain degree, shoulder function and range of motion. There is no interaction between Intervention mode and intervention time. 2021 Vol. 38 (10): 1457-1461 [Abstract] ( 39 ) HTML (1 KB)  PDF (1451 KB)  ( 56 ) 1462 Application of Lidocaine Combined with Epinephrine in Carpal Tunnel Release Surgery with WALANT YE Ming, ZHAO Qiao-yan DOI: 10.3969/j.issn.1671-7171.2021.10.006 【Objective】 To explore the value of lidocaine combined with epinephrine in carpal tunnel Release surgery with wide awake local anesthesia no tourniquet (WALANT). 【Methods】A total of 100 patients with carpal tunnel syndrome treated in our hospital from January, 2018 to January, 2021 were selected as research objects, and they were randomly divided into the observation group and the control group according to random number table method, with 50 cases in each group. The observation group received WALANT anesthesia with lidocaine combined with epinephrine, while the control group received brachial plexus block anesthesia with lidocaine alone. The operation time, blood loss, anesthesia onset time, anesthesia duration, lidocaine dosage and wrist function recovery time were recorded and compared between the two groups. We also compared Ramsay sedation (RSS) and pain visual analogue scale (VAS) scores before surgery (T0), at the end of (T1) and 30 min after (T2) operation between the two groups, The results of postoperative electromyography, excellent and good rate of carpal tunnel function recovery and complication rate were compared between the two groups. 【Results】The lidocaine dosage in the observation group was significantly less than those in the control group, and the anesthesia duration was significantly shorter than that in the control group, both with statistical significance (P<0.05).RSS and VAS scores at T1 were significantly higher than those at T0 and T2, and the difference was statistically significant (P<0.05).The electromyography parameters such as MCV(motor conduction velocity), DML, CMAP (compound muscle action potential), SCV (sensory conduction velocity), ML and SNAP (sensory nerve Action potential) of sensory nerve were compared between the two groups, and none of them showed significant difference (P>0.05).The excellent and good rates of carpal tunnel function recovery in the two groups were 84.00% and 74.00%, respectively, with no significant difference (P>0.05).The total incidence of postoperative complications in the observation group was 12.00%, which was no significantly difference with the control group (16.00%, P>0.05). 【Conclusion】Lidocaine combined with epinephrine is of high value in WALANT anesthesia for carpal tunnel loosening surgery. 2021 Vol. 38 (10): 1462-1465 [Abstract] ( 45 ) HTML (1 KB)  PDF (1180 KB)  ( 60 ) 1466 Effect of Succinylcholine Chloride on Central Venous Oxygen Saturation after Rapid Induction Endotracheal Intubation FEI Jian-ping, ZHAO Ying, JIN Wei-lin, et al DOI: 10.3969/j.issn.1671-7171.2021.10.007 【Objective】To compare the effects of depolarized muscle relaxants and non depolarized muscle relaxants on superior vena cava oxygen saturation. 【Methods】 A total of 50 patients with lower abdominal non endoscopic surgery and unilateral upper and lower limb elective surgery for non traumatic fractures were randomly divided into observation group and control group, with 25 cases in each group. Endotracheal intubation was rapidly induced by succinylcholine chloride (1.2mg/kg) and atracurium CIS benzenesulfonate (0.25 mg/kg), respectively. The superior vena cava blood was collected at the time of entry (T0), 1 min (T1), 3 min (T2), 10 min (T3) after endotracheal intubation and 10 min (T4) after operation for blood gas analysis. The changes of superior vena cava oxygen partial pressure (PcvO2), superior vena cava oxygen saturation (ScvO2), blood glucose (GLUcv) and lactic acid (LACcv) were compared between the two groups. 【Results】 There was no significant difference in PcvO2,ScvO2 ,GLUcv,LACcv between the two groups at T0 (P>0.05); At T1 , PcvO2 and ScvO2 in the control group were higher than those in the observation group (P<0.05). The incidence of mild cough, limb movement and prolonged retention time at the tracheal inlet in the control group was 40%, which was significantly higher than 8% in the observation group (χ2=8.47, P<0.05). 【Conclusion】Compared with atracurium CIS benzenesulfonate, low-dose succinylcholine induced rapid endotracheal intubation has better endotracheal intubation conditions, effectively inhibits the choking reflex caused by endotracheal intubation, and the indexes of circulation, PcvO2 and ScvO2 after endotracheal intubation are more stable, which is conducive to the balance of tissue oxygen supply and demand. 2021 Vol. 38 (10): 1466-1469 [Abstract] ( 36 ) HTML (1 KB)  PDF (1178 KB)  ( 43 ) 1470 Application of One-step Expansion Method to Establish Minimally Invasive Channel in Percutaneous Nephrolithotripsy XIE Chao-qun, LIU Xiao-bin, SU Zhou-hua, et al DOI: 10.3969/j.issn.1671-7171.2021.10.008 【Objiective】 To investigate the feasibility, safety, and clinical efficacy of one step expansion method to establish minimally invasive channel in percutaneous nephrolithotripsy.【Methods】Th clinical date of 90 patients with urinary tract stones in our hospital from January 2019 to December 2020 were analyzed retrospectively. According to different channel establishments, they were divided into one-step expansion group (the observation group) and step-by-step expansion group (the control group). There were 45 cases in the one-step group, using only F18 or F20 single fascia expansion sheath, and 45 cases in the step by step expansion group, using F5-20 fascia expansion kit. The differences in channel establishment time, operation time, blood loss, average hospital stay, cost of consumables, surgical complications, and stone removal rate were compared between the two groups. 【Results】The channel establishment time, operation time, surgical blood loss, average hospital stay, proportion of consumables in the observation group were better than those in the control group(P<0.05). There was no significant difference between the two groups in terms of average hospital stay, surgical complications and stone removal rate (P>0.05). 【Conclusions】The one step expansion method to establish minimally invasive channel in percutaneous nephrolithotripsy is safe, feasible and effective, and it can significantly shorten the channel establishment time, operation time, and reduce bleeding and consumable costs. 2021 Vol. 38 (10): 1470-1473 [Abstract] ( 41 ) HTML (1 KB)  PDF (1177 KB)  ( 64 ) 1474 Application Value of Pulmonary Ultrasound Score in Infants with Respiratory Syncytial Virus Pneumonia ZHOU Meng-jie, ZHOU Yu, LIU Jin-qiao, et al DOI: 10.3969/j.issn.1671-7171.2021.10.009 【Objective】To investigate the value of pulmonary ultrasound score in infant respiratory syncytial virus (RSV) pneumonia. 【Methods】The clinical data of 67 children with RSV pneumonia diagnosed and treated in Hunan children's Hospital from March 2020 to may 2021 were analyzed retrospectively. The correlation between pulmonary ultrasound score and disease severity and the need for respiratory support was analyzed.【Results】There was a positive correlation between the severity of RSV and pulmonary ultrasound score (P<0.05). The more serious the condition and the higher the pulmonary ultrasound score, the greater the possibility of requiring respiratory support. 【Conclusion】 Pulmonary ultrasound score can reflect the severity of RSV pneumonia, and can be used as an auxiliary method to evaluate the severity of RSV pneumonia. 2021 Vol. 38 (10): 1474-1476 [Abstract] ( 52 ) HTML (1 KB)  PDF (1604 KB)  ( 53 ) 1477 Application Value of High Parasternal Pulmonary Artery Section of Echocardiography in the Diagnosis of Chest Diseases LUO-Jia, CHEN Xiao-qiong, WANG Yue-ai, et al DOI: 10.3969/j.issn.1671-7171.2021.10.010 【Objective】 To evaluate the value of echocardiography in the diagnosis of chest diseases. 【Methods】 The ultrasonic imaging data of 3 cases of type Ⅱ (distal defect) main pulmonary septal defect (APSD), 30 cases of patent ductus arteriosus and 5 cases of thymic tumors diagnosed in our hospital from January 2013 to December 2019 were analyzed retrospectively. The application value of echocardiography in the diagnosis of chest diseases was analyzed. 【Results】Three cases of APSD type II were accurately diagnosed only on the parasternal high pulmonary artery section; In 30 cases of patent ductus arteriosus, the diameter of parasternal high pulmonary artery was significantly larger than that of parasternal large artery; Five cases of thymic tumors were located accurately in the high parasternal pulmonary artery section. 【Conclusion】The parasternal high pulmonary artery section of cardiac ultrasound is very important. It can improve the diagnosis of chest diseases and reduce missed diagnosis. It plays an important role in clinical diagnosis, treatment and operation. 2021 Vol. 38 (10): 1477-1479 [Abstract] ( 47 ) HTML (1 KB)  PDF (2103 KB)  ( 65 ) 1480 Study on the Relationship between Serum Uric Acid Level and the Risk of Cardiovascular Events in Patients with Type 2 Diabetes YAN Feng, CHANG Huan, LYU Yan, et al DOI: 10.3969/j.issn.1671-7171.2021.10.011 【Objective】To explore the relationship between serum uric acid (SUA) levels and the risk of cardiovascular events in patients with type 2 diabetes (T2DM). 【Methods】A total of 132 patients with type 2 diabetes who were admitted to our hospital from January 2017 to December 2018 were selected. According to whether a cardiovascular event occurred, patients were divided into the cardiovascular disease group (43 cases) and the non-cardiovascular disease group (89 cases).During the same period, 138 healthy people on physical examination were selected as the control group. SUA levels among groups were compared, and the correlation between the levels of SUA in T2DM patients and the occurrence of cardiovascular events was analyzed by Logistic multivariate analysis. 【Results】The level of SUA in the T2DM group was higher than that in the control group (P<0.05).The incidence of cardiovascular disease in T2DM patients was 32.58% (43/132), which was higher than 15.94% (22/132) in the control group (χ2=10.212,P=0.001).There were no significant differences in gender, age, course of diabetes, BMI, C-reactive protein (CRP), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), serum total cholesterol (TC), triglycerides (TG) and diastolic blood pressure (DBP) between the cardiovascular disease group and the non-occurring group (P>0.05).The levels of SUA, HbA1c and SBP in the cardiovascular disease group were higher than those in the non-cardiovascular disease group (P<0.05). The results of multivariate logistic regression analysis show that SUA, HbA1c and systolic blood pressure were independent risk factors for cardiovascular disease. 【Conclusion】SUA level is related to the risk of cardiovascular disease in 2T2DM patients. 2021 Vol. 38 (10): 1480-1482 [Abstract] ( 54 ) HTML (1 KB)  PDF (1160 KB)  ( 24 ) 1483 Mutation and Significance of EML4-ALK Fusion Gene in NSCLC Patients BAI Xue-mei, GUO Xuan, ZHAO Xin-han, et al DOI: 10.3969/j.issn.1671-7171.2021.10.012 【Objective】To investigate the mutation of echinoderms microtubule-associated protein-like 4-anaplastic lymphoma kinase (EML4-ALK) fusion gene in patients with non-small cell lung cancer (NSCLC) and explore its impact on the prognosis of individualized targeted therapy. 【Methods】A total of 110 NSCLC tissue specimens preserved in our hospital from February 2016 to February 2018 were selected. The mutation of EML4-ALK fusion gene was detected by real-time fluorescence quantitative PCR. The effect of the mutation on individualized targeted therapy were analyzed. 【Results】The mutation of EML4-ALK fusion gene was detected in 13 cases, the mutation rate was 11.82% (13/110). Of which, the mutation rates of EML4-ALK fusion gene were 19.23% and 23.81% in patients aged < 60 years and TNM stage Ⅲ,respectively, which were significantly higher than those in patients aged ≥ 60 years and stage Ⅰ-Ⅱ(P<0.05); The median survival time of EML4-ALK fusion gene mutation and non-mutation patients was 14.00 months (95%CI:11.71-16.29) and 13.00 months (95%CI:12.39-13.61), there was no significant difference (P> 0.05). 【Conclusion】The mutation of EML4-ALK fusion gene in NSCLC patients is related to patient's age and TNM stage, while it has no significant effect on the prognosis of individualized targeted therapy. 2021 Vol. 38 (10): 1483-1485 [Abstract] ( 40 ) HTML (1 KB)  PDF (1264 KB)  ( 58 ) 1486 A 3-year Follow-up Observation of Repeated Wheezing in Children under 5 Years Old GU Xiu-ming, DONG Wei, ZHANG Lan, et al DOI: 10.3969/j.issn.1671-7171.2021.10.013 【Objective】To investigate the onset, outcome and risk factors from 3-year follow-up of repeated wheezing in children under 5 years old. 【Methods】A total of 147 children under the age of 5 who were admitted to the Department of Pediatrics of our hospital due to recurrent wheezing were selected and followed up 3 years. According to the age of onset and remission of wheezing, the children were divided into transient wheezing group, persistent wheezing group and late-onset wheezing group. The general information, allergy-related indicators, and wheezing onset age of the three groups were compared. And the related risk factors of children with repeated wheezing were analyzed. 【Results】There was no significant difference in the number of wheezing among the three groups of children in the past 1 year when they were admitted to the hospital and the number of wheezing in the past 1 year during the follow-up (P>0.05). Children with transient wheezing did not relapse. However, the number of wheezing in the one-year follow-up in the persistent wheezing group and the late-onset wheezing group was significantly less than the number of wheezing in the past one year at admission (P<0.05).At admission, total virus positive, rhinovirus (RV) positive and respiratory syncytial virus (RSV) positive in the three groups of children were not significant (P>0.05). The previous rhinitis rates in the persistent wheezing group and late-onset wheezing group were significantly higher than that in the transient wheezing group (P<0.05),however, there was no significant difference between the persistent and late-onset wheezing group (P>0.05). The comparison of the three groups of children with the history of eczema and parental asthma did not show significant (P>0.05). The comparison of total allergen IgE was not significant (P>0.05). The serum allergen IgE of the transient wheeze group was significantly lower than that of the persistent wheezing group and the late-onset wheezing group (P<0.05),but there was no significant difference between the persistent wheeze group and the late-onset wheeze group (P> 0.05). Among the three groups of children, the positive rates of inhaled allergens and the percentage of peripheral blood eosinophils (EOS) counts from high to low were in order of transient wheeze group, persistent wheeze group to late-onset wheeze group. The differences were significant (P<0.05). However, the absolute count of EOS in the peripheral blood was not significant among three groups (P>0.05). Eczema, parental allergic rhinitis or asthma, passive smoking, viral infection, and mycoplasma infection were the main risk factors for wheezing in children. 【Conclusion】With the increase of age, the incidence of wheezing in children decreases significantly, and the outcome of wheezing is significantly correlated with allergies and rhinitis. 2021 Vol. 38 (10): 1486-1489 [Abstract] ( 46 ) HTML (1 KB)  PDF (1186 KB)  ( 423 ) 1490 Efficacy and Safety of Quetiapine Joint Midazolam in the Treatment of Alzheimer's Disease with Sleep Disturbance QIU Zheng, HE Bi- hua, LIN Li DOI: 10.3969/j.issn.1671-7171.2021.10.014 【Objective】To explore the more effective and safe way of quetiapine combined with midazolam in the treatment of Alzheimer's disease with sleep disturbance. 【Methods】A total of 127 patients with AD and sleep dysfunction were randomly divided into the observation group (n+65) and the control group (n=62). Patients in control group received quetiapine alone, while quetiapine plus midazolam were treated in the observation group. To Clinical efficacy and adverse effect between two groups were compared. 【Results】After 4 weeks of treatment, the total effective rate in the observation group was 96.92% (63/65), which was significantly higher than 80.65% (50/62) in the control group, and the difference was statistically significant (χ2=8.57, P<0.05). After 8 weeks of treatment, the total effective rate of the observation group was 100.00% (65/65), which was significantly higher than 87.10% (54/62) of the control group, and the difference was statistically significant (χ2=8.95, P<0.05). Before treatment, there were no statistically significant differences in the PSQI scores of sleep quality, time to fall asleep, daytime function, sleep disorder, sleep time, sleeping drugs, and total scores between the two groups of patients (all P>0.05). After 4 weeks and 8 weeks of treatment, the observation group's PSQI scores for sleep quality, time to fall asleep, daytime function, sleep time, sleeping drugs and other scores and total scores were significantly lower than those of the control group; And the differences were statistically significant (both P<0.05). However, there was no statistically significant difference in sleep disorder scores between the two groups (P>0.05). The total incidence of adverse reactions in the observation group was 3.07% (2/65), which was significantly lower than 14.52% (9/62) in the control group, and the difference was statistically significant (χ2=5.249, P=0.028).【Conclusion】Quetiapine combined with midazolam in the treatment of Alzheimer's disease with sleep disturbance was more effective and safer than singular quetiapine treatment and it was worth of application. 2021 Vol. 38 (10): 1490-1492 [Abstract] ( 41 ) HTML (1 KB)  PDF (1155 KB)  ( 33 ) 1493 Efficacy and Safety of Different Combinations of Mifepristone in the Induced Labor of the Second Trimester of Scarred Uterus WU Qun-ying, WANG Xiao-yu DOI: 10.3969/j.issn.1671-7171.2021.10.015 【Objective】To compare the efficacy and safety of different combinations of mifepristone in the induction of labor in the second trimester of scarred uterus. 【Methods】A retrospective analysis of 90 cases of women in second trimester of scarred uterus who received labor induction in Weinan Central Hospital from December 2015 to December 2019 was conducted. Patients were divided into the mifepristone + double balloon group (28 cases, group A) ,mifepristone + carprost methyl ester group(32 cases, group B) and mifepristone + esacridine lactate group (30 cases, group C) according to the method of labor induction. The cervical Bishop score, labor induction effect, labor induction bleeding and complications of the three groups were compared. 【Results】After induction of labor, the cervical Bishop score of the mifepristone + double balloon group was > the mifepristone + carprost methyl group>the mifepristone + esacridine lactate group; the difference was statistically significant (P<0.05). The effective rate of the mifepristone + esacridine lactate group was lower than that of the mifepristone + double balloon group and the mifepristone + carboprost methylester group, while the efficiency rate of the mifepristone + esacridine lactate group was higher than that of the mifepristone + double balloon group and the mifepristone + carprost methyl ester group. The differences were statistically significant (P<0.05). However, there was no statistically significant difference in the effective rate and successful induction rate among the three groups (P> 0.05). The time from the beginning of the birth process to the delivery of the fetus plus the total induction time in mifepristone + esacridine lactate group was longer than those in the mifepristone + double balloon group and the mifepristone + carboprost methylester group (P<0.05). There were no statistically significant differences in the bleeding volume, the time from inducing labor to effective contractions, the incidence of complications and the incidence of adverse reactions in the three groups (P> 0.05). 【Conclusion】The combination of mifepristone with balloon, carprost methyl ester and ethacridine lactate is feasible in the induction of labor in the second trimester of scar uterus, however, the combination of mifepristone with double balloon has higher cervical maturity and shorter induction time. 2021 Vol. 38 (10): 1493-1496 [Abstract] ( 41 ) HTML (1 KB)  PDF (1178 KB)  ( 56 ) 1497 Effect of Botulinum Toxin Type A Combined with Routine Rehabilitation on Balance Ability and Daily Living Ability of Children with Spastic Hemiplegic Cerebral Palsy YUAN Li-ping, HE Jin-hua, HU Ji-hong, et al DOI: 10.3969/j.issn.1671-7171.2021.10.016 【Objective】To investigate the effect of botulinum toxin a combined with routine rehabilitation therapy on balance ability and daily living ability of children with spastic hemiplegic cerebral palsy. 【Methods】A total of 57 children with spastic hemiplegia treated in the rehabilitation center of Hunan children's Hospital from January 2017 to June 2020, including 38 males and 19 females, was divided into control group (n=26, given routine rehabilitation treatment) and observation group (n=31, injected with botulinum toxin a combined with routine rehabilitation treatment) according to the wishes of their families. The children in the two groups were treated for 3 months. The spasticity degree, balance ability and quality of life of the two groups were evaluated by comprehensive spasticity scale (CCS), Berg Balance Scale (BBS) and modified Barthel index scale before and after treatment. 【Results】 Before treatment, there was no significant difference between the two groups (P>0.05); After treatment, the scores of comprehensive spasm scale in the two groups were lower than those before treatment, and the observation group was lower than that in the control group, the difference was statistically significant (mean P<0.05). Before treatment, there was no significant difference in Berg Balance Scale score and ADL score between the two groups (P>0.05); After treatment, the Berg Balance Scale score and ADL score of the two groups were higher than those before treatment, and the observation group was higher than that of the control group, the difference was statistically significant (mean P<0.05). 【Conclusion】 Botulinum toxin a combined with routine rehabilitation treatment for children with spastic hemiplegic cerebral palsy can significantly improve the balance function and daily living ability of children with spastic hemiplegic cerebral palsy, with high safety, which can be popularized in clinic. 2021 Vol. 38 (10): 1497-1499 [Abstract] ( 36 ) HTML (1 KB)  PDF (1153 KB)  ( 27 ) 1500 Characteristics of Glucose Metabolism and Related Factors in Patients with Primary Aldosteronism HUANG Su-lan, GUO Ning, LIANG Li, et al DOI: 10.3969/j.issn.1671-7171.2021.10.017 【Objective】To explore the related factors of abnormal glucose metabolism in patients with primary aldosteronism (PA). 【Methods】The clinical data of 195 patients with PA first diagnosed in the Department of Cardiology of our hospital from January 2015 to December 2019 (observation group) were analyzed retrospectively, and 195 patients with essential hypertension were included as the control group. The blood glucose metabolism indexes, blood lipid level, consulting room blood pressure and ambulatory blood pressure of the two groups were compared. The effects of renin, aldosterone and aldosterone/renin activity ratio (ARR) on blood glucose metabolism indexes were analyzed by multiple linear regression. 【Objective】The course of hypertension, body mass index (BMI), the proportion of abnormal glucose metabolism, fasting blood glucose (FPG), glycosylated hemoglobin (HbAc1), K, aldosterone and ARR in the observation group were higher than those in the control group, and the renin level and the proportion of women in the observation group were lower than those in the control group (P<0.05). The consulting room blood pressure, 24-hour blood pressure, daytime and nighttime blood pressure in the observation group were higher than those in the control group, and the decrease rate of nighttime systolic blood pressure was lower than that in the control group (P<0.05). The proportion of FPG and abnormal glucose metabolism and diabetes in patients with PA adrenal hyperplasia were higher than those in adrenal adenomas (P<0.05). Multiple linear regression analysis showed that FPG and HbAc1 levels were independently correlated with aldosterone levels (P<0.05). 【Conclusion】The blood glucose level in PA patients is higher than that in pH patients. The aldosterone level is independently related to the increase of FPG and HbAc1. 2021 Vol. 38 (10): 1500-1503 [Abstract] ( 31 ) HTML (1 KB)  PDF (1186 KB)  ( 49 ) 1504 Prognostic Value of sTREM-1 Combined with CC16 in Elderly Patients with Chronic Obstructive Pulmonary Disease WEI Dan, CHEN Juan DOI: 10.3969/j.issn.1671-7171.2021.10.018 【Objective】To investigate the predictive effect of soluble myeloid cell trigger receptor-1 (sTREM-1) combined with Clara cell secretory protein (CC16) on poor prognosis in elderly patients with acute exacerbation of chronic obstructive pulmonary disease (COPD). 【Methods】A total of 206 patients with COPD treated in our hospital from February 2019 to March 2020 was selected, including 108 patients with acute exacerbation of COPD (AECOPD) (group A) and 98 patients with stable COPD (group B). In addition, 95 healthy volunteers (Group C) who underwent physical examination in our hospital at the same time were selected. The levels of serum sTREM-1 and CC16 in the three groups were compared; The incidence of poor prognosis in AECOPD patients was counted and divided into poor prognosis group and good prognosis group according to the prognosis; The clinical data of poor prognosis group and good prognosis group were compared; Logistic regression analysis was used to analyze the prognostic factors of AECOPD patients; The receiver operating characteristic curve (ROC) was made to analyze the predictive efficacy of sTREM-1 combined with CC16 in the poor prognosis of elderly AECOPD patients. 【Results】 The level of sTREM-1 in the group A and the group B was significantly higher than that in then group C (P<0.05), and the level of CC16 was significantly lower than that in the group C (P<0.05); The level of sTREM-1 in the group A was significantly higher than that in the group B (P<0.05), and the level of CC16 was significantly lower than that in the group B (P<0.05); The incidence of poor prognosis in AECOPD patients was 25.93% (28/108); The mechanical ventilation time, procalcitonin (PCT), C-reactive protein (CRP) and sTREM-1 levels in the poor prognosis group were significantly higher than those in the good prognosis group (P<0.05), while the vital capacity (FVC), forced expiratory volume in the first second (FEV1) and CC16 levels in the poor prognosis group were significantly lower than those in the good prognosis group (P<0.05); Logistic regression showed that CRP, sTREM-1 and CC16 were the influencing factors of poor prognosis in patients with AECOPD (P<0.05); ROC analysis showed that the best cutoff points for predicting the poor prognosis of elderly AECOPD patients by the levels of sTREM-1 and CC16 were 161.25 ng/L and 65.83μg/L , respectively; The specificity of the combination of sTREM-1 and CC16 was 96.25%, which was significantly higher than that of serum sTREM-1 and CC16 alone. The area under the curve (AUC) of the combination of sTREM-1 and CC16 in predicting the poor prognosis of elderly AECOPD patients was 0.923, which was higher than that of serum sTREM-1 and CC16 alone (P<0.05). 【Conclusion】sTREM-1 combined with CC16 is effective in predicting the poor prognosis of elderly AECOPD patients, and can be used to predict the poor prognosis of elderly AECOPD patients. 2021 Vol. 38 (10): 1504-1507 [Abstract] ( 40 ) HTML (1 KB)  PDF (1217 KB)  ( 24 ) 1508 Clinical Analysis of Transcranial Direct Current Stimulation Combined with Non Convulsive Electroconvulsive and Conventional Antidepressants in the Treatment of Severe Depression LI Xiao-ling, LIAO Hai-rong, LIANG Jia-quan, et al DOI: 10.3969/j.issn.1671-7171.2021.10.019 【Objective】To investigate the clinical efficacy of transcranial direct current stimulation (tDCS) combined with non convulsive electroconvulsive (MECT) and conventional antidepressants in the treatment of severe depression (MDD), and to analyze the effects on the levels of serum brain-derived neurotrophic factor (BDNF) and neuropeptide (NPY). 【Methods】 A total of 110 patients with MDD treated in our hospital from March 2017 to October 2020 was randomly divided into observation group (n=57) and control group (n=53). Both groups were treated with conventional western medicine and MECT. The observation group was treated with transcranial DC stimulation on the basis of the control group. Hamilton Depression Scale (HAMD) was used to evaluate the clinical efficacy before and after treatment. Wechsler artificial memory scale and Wechsler Adult Intelligence Scale were used to evaluate the cognitive function of the two groups before and after treatment, and the levels of serum brain-derived neurotrophic factor (BDNF) and neuropeptide (NPY) were compared between the two groups. 【Results】 After treatment, the HAMD score of the two groups was significantly lower than that before treatment, and that of the observation group was lower than that of the control group (P<0.05). The total effective rate in the observation group was 92.98% (53/57), which was higher than 77.36% (41/53) in the control group (χ2=5.394, P=0.02 < 0.05). After treatment, the WMS and WAIS-RC scores of the two groups were higher than those before treatment, and the observation group was higher than that of the control group (P<0.05); The level of serum BDNF in the two groups was higher than that before treatment, and the level of serum NPY was lower than that before treatment, and there was significant difference between the observation group and the control group (all P<0.05). 【Conclusion】 tDCS combined with MECT and conventional antidepressants is effective in the treatment of MDD. It can adjust the levels of serum BDNF and NPY and effectively improve the cognitive function of patients, which is worthy of clinical reference. 2021 Vol. 38 (10): 1508-1511 [Abstract] ( 41 ) HTML (1 KB)  PDF (1183 KB)  ( 78 ) 1512 Clinical Effect of Transurethral Plasma Resection of Prostate and Vaporization Resection of Prostate in the Treatment of Benign Prostatic Hyperplasia Complicated with Chronic Prostatitis SONG Xiao-lin, WANG Wen-zhen DOI: 10.3969/j.issn.1671-7171.2021.10.020 【Objective】To investigate the clinical effect of transurethral plasma resection of prostate and vaporization resection of prostate in the treatment of benign prostatic hyperplasia complicated with chronic prostatitis. 【Methods】A total of 80 patients with benign prostatic hyperplasia complicated with chronic prostatitis treated in Jinan Fifth People's Hospital from August 2018 to August 2020 was randomly divided into observation group (transurethral plasma resection of prostate) and control group (vaporization resection of prostate), with 40 cases in each group. The general conditions of operation and the incidence of complications were observed. The decreased values of hemoglobin (HB) and hematocrit, international prostate symptom (IPSS) score and maximum urinary flow rate (Qmax) were compared between the two groups before and after operation. 【Results】 The total blood loss and average blood loss in the control group were lower than those in the observation group, the difference was statistically significant (P<0.05). There was no significant difference in operation time and total prostate resection between the two groups (P>0.05). The decrease of Hb and hematocrit in the control group were lower than those in the observation group (P<0.001). Six months after operation, the IPSS score of the control group was lower than that of the observation group, and the Qmax was higher than that of the observation group (mean P<0.05). There was no significant difference in the incidence of postoperative bleeding, bladder irritation, urethral stricture and capsule perforation between the two groups(χ2=1.053,P=0.305＞0.05). 【Conclusion】 Vaporization electroresection of prostate is effective in the treatment of patients with benign prostatic hyperplasia complicated with chronic prostatitis. It can significantly improve the symptoms of patients without increasing the incidence of complications. It has good application value. 2021 Vol. 38 (10): 1512-1514 [Abstract] ( 30 ) HTML (1 KB)  PDF (1155 KB)  ( 60 ) 1515 Effects of Acute Cerebral Infarction on the Levels of Dendritic Cells and Inflammatory Factors in Rats ZHONG Zhen-zhen, XU Ping, WANG Kai, et al DOI: 10.3969/j.issn.1671-7171.2021.10.021 【Objective】 To to study the effects of acute cerebral infarction on the levels of dendritic cells (DCS) and inflammatory factors in rats.【Methods】 Twenty 8-week-old female SD rats were randomly divided into experimental group and control group, with 10 rats in each group. The middle cerebral artery occlusion (MACO) rat model was made by suture method. The operation steps of the control group were the same as those of the experimental group, but the suture was not inserted. Immunofluorescence, immunohistochemistry and ELISA were performed 7 days after operation. Expression levels of DCs and interleukin-1β (IL-1 β), Interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) in the experimental group and the control group were observed.【Results】 Compared with the control group, the quantity of positive cells of CD74 and integrinαE in the ischemic brain tissue of the experimental group was bigger, and the difference was statistically significant (P<0.05);The expression of IL-1β,IL-6 and TNF-α in ischemic brain tissue of rats in experimental group was significantly higher than that in the control group (P<0.05);The difference of the contents of serum IL-1β,IL-6 and TNF- α of the two groups was statistically significant (P<0.05). 【Conclusion】Cerebral infarction induces the expression of DCs and inflammatory factors in rats, and the inflammatory response is a pathological process of acute cerebral infarction. 2021 Vol. 38 (10): 1515-1518 [Abstract] ( 42 ) HTML (1 KB)  PDF (2221 KB)  ( 58 ) 1519 Value of Preserving Intercostal Brachial Nerves in Reducing the Postoperative Discomfort During Radical Mastectomy ZHAO Yu-bin, XU Chao DOI: 10.3969/j.issn.1671-7171.2021.10.022 【Objective】To evaluate the value of preserving intercostal brachial nerves in reducing the postoperative discomfort during radical mastectomy. 【Methods】 The clinical data of 90 patients with breast cancer admitted in our hospital from February 2017 to March 2019 were retrospectively analyzed. All patients underwent radical mastectomy. According to the axillary lymph node metastasis, the patients were divided into the observation group (preserving intercostal brachial nerve) and the control group (without intercostal brachial nerve), with 45 cases in each group. The operation condition, comfort, complication rate and sensory disturbance of upper arm were compared between the two groups. 【Results】There was no significant difference in the average operation time, the number of lymph nodes and the amount of intraoperative bleeding between the two groups (P>0.05). The incidence of complications in the observation group was 13.33% (6/45), lower than 15.56% (7/45) in the control group, but the difference was not statistically significant(χ2=0.090,P=0.764＞0.05). The incidence of upper arm sensory impairment in the observation group was lower than that in the control group at 1 week and 1, 3 and 6 months after operation (P<0.05). The scores of physiology, psychology, social culture and environment in the observation group were significantly higher than those in the control group (P<0.05). 【Conclusion】 Reserving intercostal brachial nerve in radical mastectomy for breast cancer patients can effectively improve the postoperative quality of life, reduce the incidence of postoperative upper arm medial sensory disturbance, and improve postoperative comfort. 2021 Vol. 38 (10): 1519-1521 [Abstract] ( 49 ) HTML (1 KB)  PDF (1151 KB)  ( 38 ) 1522 Effects of Bone Marrow-derived Mesenchymal Stem Cells on Dendritic Cells in Patients with Multiple Myeloma MA Yan-yan DOI: 10.3969/j.issn.1671-7171.2021.10.023 【Objective】To investigate effects of multiple myeloma (MM) patients with bone marrow derived mesenchymal stem cell (MSC) on differentiation, maturation and cytokinese cretion of dendritic cell (DC) and its effect on DC helper T cell skilling myeloma cells. 【Methods】MSC cells from MM patients and healthy volunteers were mixed and cultured with DC, T cells and myeloma cell line U266 cells. DC immunophenotype was detected by flow cytometry, and cell apoptosis were measured by Annexin V-FITC/PI double labeling. 【Results】Culture supernatant TGF-β1 level of healthy persons' MSC cells was (60.52±2.83)pg/mL, which was significantly lower than that in MM patients (302.51±70.52) pg/mL; Tthe difference was statistical significance (P<0.05). Co-culture of MSC and immature/ mature DC cells showed that CD1a,CD11C,CD80,CD83,CD86, IL-12 secretion and HLA-DR expression were significantly suppressed(P<0.05). When T cells+ DC+ U266 were cultured together, the apoptosis rate in U266 cells was (72.24±8.63)%; While co-culturing T cell+DC+U266+ MSC from MM patients, the apoptosis rate in U266 cells was (45.56±9.22)%; When mixing T cell+DC,U266 cells with MSC from healthy persons, the apoptosis rate in U266 cells was (25.53±7.31)%. All differences were statistically significant (P<0.05). 【Conclusion】MSC from MM patients can significantly inhibit DC differentiation, maturation and cytokine secretion, and inhibit the killing effect of DC helper T cells. 2021 Vol. 38 (10): 1522-1525 [Abstract] ( 34 ) HTML (1 KB)  PDF (1712 KB)  ( 119 ) 1526 Analysis of Drug Resistance and Related Drug Resistance Genotyping of Pseudomonas Aeruginosa MA Ai-kuang, GAO Hua-li, WANG Min, et al DOI: 10.3969/j.issn.1671-7171.2021.10.024 【Objective】 To analyze the drug resistance and related drug resistance genotypes of Pseudomonas aeruginosa (PA). 【Methods 】 A total of 270 PA strains isolated from the laboratory department of Shanghai Electric Power Hospital were collected, the sensitivity of PA to 15 commonly used antibiotics was analyzed, and the expression of mexxy and mexab resistance genes was detected by RT-PCR. 【Results】The elderly aged 60 and above were most infected with Pseudomonas aeruginosa, 221 people, accounting for 81.86%, followed by 37 cases aged 30 ~ 60, accounting for 13.70%, and 12 cases aged 30 and below, accounting for 4.44%. The main source of PA was sputum, the detection rate was 47.04%, followed by urine (26.67%), wound (11.85%) and pharyngeal swab (11.85%). PA is mainly distributed in respiratory medicine, urology and neurology, of which 81 are most widely distributed in respiratory medicine, accounting for 30.00%. The resistance rates of PA to antibiotics from high to low were ticarcillin/clavulanic acid (24.07%), ciprofloxacin (23.70%), levofloxacin (22.96%), piperacillin (22.59%), ceftazidime (21.86%), imipenem (19.26%), aztreonam (19.26%), meropenem (18.52%), gentamicin (15.93%), tobramycin (15.56%) and cefepime (11.11%) , Cefoperazone/sulbactam (11.11%), piperacillin/tazobactam (10.37%), amikacin (8.15%) and polymyxin B (0.00%). Mexxy was expressed in 30% of PA strains, mexab was expressed in 42% of strains, and 16% of them were co expressed.【Conclusion】The elderly aged 60 and above in respiratory department ward are most vulnerable to PA infection. Overexpression of mexxy and mexab in active efflux system is one of the mechanisms of PA multidrug resistance. 2021 Vol. 38 (10): 1526-1529 [Abstract] ( 36 ) HTML (1 KB)  PDF (1175 KB)  ( 41 ) 1530 Analysis of Cardiopulmonary Resuscitation between Abdominal Pressure Lifting Device and Barehanded Chest Compressions SUN Mu, ZHAO Jia-jun DOI: 10.3969/j.issn.1671-7171.2021.10.025 【Objective】To explore the effect of cardiopulmonary resuscitation (CPR) between abdominal pressure lifting device and hands-only chest compressions on prehospital cardiopulmonary resuscitation.【Methods】A total of 80 patients with cardiopulmonary resuscitation who were treated with cardiac arrest from January 2017 to 2020 were selected. According to the different methods of cardiopulmonary resuscitation before sending to hospital, they were divided into the hands-only CPR group (A group) and the CPR group with abdominal pressure lifting device (B group), with 40 cases in each group. The effects of cardiopulmonary resuscitation in the two groups were analyzed and compared. 【Results】The mean arterial pressure, heart rate, arterial PcO2 and end-expiratory PcO2 in the B group were significantly higher than those in the A group (P<0.05). The pulmonary oxygen partial pressure and blood lactic acid in the B group were significantly lower than those in the A group (P<0.05). After treatment, the time from cardiac arrest to spontaneous respiration in the B group was significantly shorter than that in the A group, and the CPR success rate, spontaneous circulation recovery rate, 24-hour survival rate and survival discharge rate of the B group were significantly higher than those of the A group (P<0.05). 【Conclusion】Compared to hands-only CPR with chest compressions, CPR with abdominal pressure lifting device is more beneficial to increase pulmonary ventilation, better hemodynamic effect, better recovery rate of spontaneous circulation and success rate of CPR, and better prognosis. 2021 Vol. 38 (10): 1530-1532 [Abstract] ( 31 ) HTML (1 KB)  PDF (1152 KB)  ( 43 ) 1533 Correlation between Th1, Th2 Cells, Cytokines and Pulmonary Function in Children with Respiratory Syncytial Virus Pneumonia LEI Rong, SHEN Jie DOI: 10.3969/j.issn.1671-7171.2021.10.026 【Objective】To investigate the correlation between helper T cells (Th) 1, Th2 cells, cytokines and lung function in children with respiratory syncytial virus (RSV) pneumonia. 【Methods】A total 80 children with respiratory syncytial virus pneumonia treated in our hospital from November 2017 to December 2020 were selected as RSV group, and 52 infants who underwent physical examination in our hospital at the same time were selected as control group. The percentages of Th1 and Th2 cells in peripheral blood of the two groups were detected by flow cytometry, and Th1/Th2 were calculated; the cytokine interferon (IFN-γ) and interleukin (IL) - 4 levels secreted by Th1 and Th2 cells was detected by enzyme-linked immunosorbent assay (ELISA) ; the pulmonary function of the two groups was detected by pulmonary function tester, and the Th1/Th2 and serum IFN-γ,IL-4 level and pulmonary function index were compared between the two groups; Pearson was used to analyze the correlation between Th1/Th2 ,serum IFN-γ, IL-4 level and pulmonary function indexes. 【Results】 There was no significant difference in gender, age and body mass index (BMI) between the two groups (P>0.05); Percentage of Th1 cells, Th1/Th2 and IFN-γ in peripheral blood of the RSV group. The percentage of Th2 cells and IL-4 in peripheral blood were significantly higher than those in the control group (P<0.05); Minute ventilation (MV), tidal volume (VT), inspiratory time (TI), expiratory time (TE), respiratory ratio (Ti/TE) and peak expiratory flow rate (PEF) in the RSV group were significantly lower than those in the control group, and respiratory rate (RR) was significantly higher than those in the control group (P<0.05); Th1/Th2 was positively correlated with MV, VT, TE and PEF (r=0.86, 0.57, 0.34, 0.67, P<0.05), and negatively correlated with RR (r=-0.69, P<0.05); IFN-γ was positively correlated with VT, Ti/TE and PEF (r=0.47, 0.64, 0.70, P<0.05), and negatively correlated with RR (r=-0.81, P<0.05); IL-4 was negatively correlated with MV, VT, Ti/TE and PEF (r=-0.47, -0.60, -0.32, -0.63, P<0.05). 【Conclusion】 Th2 dominant response is the main manifestation of immune response in children with RSV pneumonia. Th1, Th2 cells and their secreted cytokines are significantly correlated with pulmonary function indexes, which can be used as indexes for judging the condition and evaluating the prognosis. 2021 Vol. 38 (10): 1533-1536 [Abstract] ( 36 ) HTML (1 KB)  PDF (1185 KB)  ( 50 ) 1537 Clinical Effect of Cerebrospinal Fluid Replacement in the Treatment of Subarachnoid Hemorrhage in Children ZHANG Jin-wei, WU Shui-hua, SU Jun, et al DOI: 10.3969/j.issn.1671-7171.2021.10.027 【Objective】To investigate the clinical effect of cerebrospinal fluid replacement in the treatment of subarachnoid hemorrhage in children.【Methods】 A total of 98 children with subarachnoid hemorrhage treated in our hospital from June 2019 to January 2021 was randomly divided into observation group and control group, with 49 cases in each group. The children in the control group were given routine treatment, and the children in the observation group were treated with cerebrospinal fluid replacement on the basis of routine treatment. The recovery of consciousness, clinical neurological deficit, complications and ability of daily living were compared between the two groups. 【Results】 The recovery time of neck stiffness and consciousness in the observation group were lower than those in the control group. The proportion of cerebrospinal fluid returning to normal after one week of treatment was higher than that in the control group (P<0.05). The proportion of children with nerve defect in the observation group was 6.12% (3/49), which was significantly lower than 20.41% (10/49) in the control group(χ2=4.346,P=0.037＜0.05).The incidence of complications in the observation group was 8.16% (4/49), which was lower than 22.45% (11/49) in the control group(χ2=3.857,P=0.049＜0.05).After treatment, the scores of physiological function, physiological function, physical pain, general health and energy in the two groups were higher than those before treatment, and the scores in the observation group were higher than those in the control group (P<0.05). 【Conclusion】 The clinical effect of cerebrospinal fluid replacement in the treatment of subarachnoid hemorrhage in children is significant, which can effectively improve the nerve defect and reduce the incidence of complications. 2021 Vol. 38 (10): 1537-1539 [Abstract] ( 35 ) HTML (1 KB)  PDF (1159 KB)  ( 22 ) 1540 Related Influencing Factors of Arch Height in Patients with High Myopia after PPC-ICL Implantation ZHANG Xue, LI Hai-yan, MA Bo, et al DOI: 10.3969/j.issn.1671-7171.2021.10.028 【Objective】To explore the influencing factors of arch height changes in patients with high myopia after phakic posterior chamber implantable contact lens (PPC-ICL) implantation. 【Methods】Patients with high myopia who underwent PPC-ICL implantation in our hospital from May 2016 to January 2018 were recruited as study subjects, including 56 cases (58 eyes) with high arch height (＞750 μm, the high arch height group) and 64 cases (76 eyes) with low arch height (＜250 μm, the low arch height group). Meanwhile, 140 patients (140 eyes) with normal arch height were selected as the healthy control group. The preoperative related parameters were compared among the three groups. Multivariate regression analysis of arch height changes in patients with high myopia after PPC-ICL implantation was performed. 【Results】There were no significant differences in age, spherical equivalent (SE), height of ciliary processes (T value) and mean keratometry (Km) among the three groups (P>0.05), however, the differences in white-to-white distance (WTW), posterior angle, anterior chamber depth (ACD), horizontal distance of sulcus-to-sulcus (STS), lens thickness (LT), ICL size and axial length (AL) were statistically significant (P<0.05) among groups. Multivariate logistic regression analysis showed that WTW, ACD, horizontal and vertical distance of STS, LT and ICL size were independent influencing factors of abnormal arch height (P<0.05). SE and horizontal distance of STS were independent influencing factors of arch height variation after PPC-ICL implantation (P<0.05). 【Conclusion】The abnormal arch height in high myopia patients after PPC-ICL implantation is related to multiple parameters such as preoperative WTW, ACD, STS, LT and ICL size. The arch height variation is closely related to SE and horizontal distance of STS. 2021 Vol. 38 (10): 1540-1543 [Abstract] ( 75 ) HTML (1 KB)  PDF (1180 KB)  ( 146 ) 1544 Correlation between Changes of Serum GDF-15, Lp-PLA2 and Cardiac Function and Ventricular Remodeling in Patients with CHF GE Zhen-zhong, GONG Zhao-wei, NING Yi, et al DOI: 10.3969/j.issn.1671-7171.2021.10.029 【Objective】 To investigate the relationship between the changes of serum growth differentiation factor-15 (GDF-15) and lipoprotein associated phospholipase A2 (Lp-PLA2) and cardiac function and ventricular remodeling in patients with chronic heart failure (CHF). 【Methods】 A total of 140 patients with CHF Treated in Wuxi People's Hospital from January 2017 to March 2019 (observation group) and 70 healthy volunteers in the same period (control group). The levels of serum GDF-15, Lp-PLA2, N-terminal pro brain natriuretic peptide (NT-proBNP) and cardiac function indexes [left ventricular ejection fraction (LVEF%), left ventricular short axis shortening (FS%) and left ventricular mass index (LVMI)] were compared between the two groups, and their correlation was analyzed.【Results】The levels of serum GDF-15, Lp-PLA2 and NT-proBNP in the observation group were significantly higher than those in the control group (P<0.05). The level of LVMI in the observation group was significantly higher than that in the control group, and the levels of LVEF and FS were significantly lower than that in the control group (P<0.05). The levels of serum GDF-15 and Lp-PLA2 in CHF patients with NYHA grade Ⅱ, Ⅲ and Ⅳ increased gradually, and there were significant differences between the groups (mean P<0.05). The levels of serum GDF-15 and Lp-PLA2 in CHF were significantly negatively correlated with LVEF and FS (P<0.05), and positively correlated with LVMI and serum NT-proBNP (P<0.05). 【Conclusion】 The levels of serum GDF-15 and Lp-PLA2 in patients with CHF are significantly increased, which are closely related to cardiac function and ventricular remodeling. 2021 Vol. 38 (10): 1544-1546 [Abstract] ( 33 ) HTML (1 KB)  PDF (1159 KB)  ( 40 ) 1547 Experimental Study of Astragaloside IV Regulating p38 MAPK Signaling Pathway in the Treatment of Renal Tubulointerstitial Fibrosis ZHOU Shu-juan, LI Wei DOI: 10.3969/j.issn.1671-7171.2021.10.030 【Objective】To investigate the effect of astragaloside Ⅳ(AS-Ⅳ) regulating p38 mitogen activated protein kinase (MAPK) pathway on renal tubulointerstitial fibrosis.【Methods】 The animal model of tubulointerstitial fibrosis induced by unilateral ureteral ligation (UUO) was established in C57BL/6 mice and divided into sham operation group (group A), model group (group B) and treatment group (Group C). Group C was gavaged with 20 mg/(kg·d) as-iv for 7 days, and group A and B were gavaged with the same dose of physiological salt water. After 2 weeks, the pathological changes of the kidney were observed (Masson staining), and the transforming growth factor was detected by real-time quantitative TGF-β1 mRNA expression by PCR. According to different cell culture media, NRK-49F cells were divided into negative control group ,TGF-β1 Group (with TGF-β1 10 ng/mL stimulated cells), TGF-β1 + AS-Ⅳ group (before TGF-β1 stimulation, add doses of 50, 100 and 200 μg/mL AS-Ⅳ treatment for 1 h respectively), and the level of p-p38 protein in NRK-49F cells was observed (Western method).【Results】 The relative expression of TGF-β1 mRNA in renal tissue of group A , B and C was 2.65±0.38, 28.71±4.05 and 20.29±3.10, and the group A was at a lower level. Compared with the group A, the renal TGF -β1 mRNA expression in the group B and C was significantly higher than that in the group A (P<0.01), the expression in the group C was significantly lower than that in the group B(P<0.05). The results of in vitro test showed that p-p38 protein level, TGF-β1 GrouP>negative control group (P<0.01), TGF-β1 GrouP>TGF-β1 + AS-Ⅳ group (P<0.01), TGF-β1 + AS-Ⅳ group 200 μg/mL< 100 μg/mL<50 μg/mL(P<0.01). 【Conclusion】AS-Ⅳ can reduce TGF-β1 level, reduce renal tubulointerstitial fibrosis, and its protective effect may be related to the inhibition of p38 MAPK phosphorylation by AS-Ⅳ. 2021 Vol. 38 (10): 1547-1549 [Abstract] ( 32 ) HTML (1 KB)  PDF (1675 KB)  ( 31 ) 1550 Correlation Between Plasma Alkaline Phosphatase Level and Hemorrhagic Transformation of Cerebral Infarction YU Da-lin, XIAO Ling, TAN Si-yi, et al DOI: 10.3969/j.issn.1671-7171.2021.10.031 【Objective】To investigate the correlation between different alkaline phosphatase (ALP) levels and hemorrhagic transformation of cerebral infarction. 【Methods】 A total of 718 patients with cerebral infarction hospitalized in our hospital from January 2018 to January 2020 were selected. The ALP level was measured within 48 hours and the general baseline data were collected. According to the European collaborative study on acute stroke (ECASS Ⅲ), the hemorrhagic transformation (HT) of cerebral infarction was divided into hemorrhagic cerebral infarction (HI,n=33) and cerebral parenchymal hemorrhage (PH,n=123). Among them, the aggravation of neurological dysfunction caused by hemorrhagic transformation (NIHSS score increased by ≥ 4 points) was defined as symptomatic intracranial hemorrhage (sICH). The differences of ALP levels between hemorrhagic transformation group (HTG group) and non hemorrhagic transformation group (NHTG group) were compared. The correlation between different ALP levels and different types of hemorrhagic transformation was evaluated and analyzed by Quantile logistic regression analysis. 【Results】 There was no significant difference in the level of ALP between HI group, pH group and nhtg group (P>0.05). The level of ALP in the sICH group was significantly higher than that in NHTG group (P<0.05). Quantile regression analysis showed that different levels of ALP had no correlation with hi and pH, while high levels of ALP had significant correlation with sICH (P<0.05). 【Conclusion】Patients with cerebral infarction combined with high-level ALP are more prone to sICH transformation. 2021 Vol. 38 (10): 1550-1552 [Abstract] ( 31 ) HTML (1 KB)  PDF (1162 KB)  ( 24 ) 1553 Clinical Efficacy of Tiapride Hydrochloride Tablets Combined with Wuling Capsule in the Treatment of Alcoholic Mental Disorder ZHANG Cai-ping, SHEN Hui-zhen, FANG Fang DOI: 10.3969/j.issn.1671-7171.2021.10.032 【Objective】To investigate the clinical efficacy of tiapride hydrochloride tablets combined with Wuling capsule in the treatment of alcoholic mental disorder. 【Methods】 A total of 86 patients with alcoholic mental disorders treated in our hospital from March 2017 to July 2020 was randomly divided into observation group (treated with tiapride hydrochloride tablets combined with Wuling capsule) and control group (treated with tiapride hydrochloride tablets), with 43 cases in each group. The efficacy, positive and Negative Syndrome Scale score (PANSS), depression (SDS), self rating Anxiety Scale score (SAS), P300 parameters and safety were compared between the two groups. 【Results】 The total effective rate of the observation group was 90.70% (39/43), which was higher than 72.09% (31/43) of the control group(χ2=4.914,P=0.027＜0.05). After treatment, the scores of PANSS positive symptoms, negative symptoms, general psychopathological symptoms, SDS and SAS in the two groups were lower than those before treatment, and the observation group was lower than that in the control group (P<0.05); The latency of P300 parameters N1, N2 and P3 in the two groups were lower than those before treatment, and the amplitudes of N1, N2 and P3 were higher than those before treatment (P<0.05); The latency of P300 parameters N1, N2 and P3 in the observation group were lower than those in the control group, and the amplitudes of N1, N2 and P3 in the observation group were higher than those in the control group (P<0.05). The total incidence of adverse reactions in the observation group was 2.33% (1/43), which was lower than 9.30% (4/43) in the control group, but the difference was not statistically significant( χ2 =0.849,P=0.357＞0.05). 【Conclusion】 Tiapride hydrochloride tablets combined with Wuling capsule is effective in the treatment of alcoholic mental disorder, can reduce the symptoms of anxiety and depression, and has good safety. 2021 Vol. 38 (10): 1553-1555 [Abstract] ( 29 ) HTML (1 KB)  PDF (1155 KB)  ( 110 ) 1556 Effect of Paclitaxel Combined with Cisplatin Intraperitoneal Circulation Thermoperfusion Chemotherapy on Tumor Markers and Prognosis in Patients with Ovarian Cancer CUI Hong-jun, XIN Xiao-sen, ZHANG Shu-ze, et al DOI: 10.3969/j.issn.1671-7171.2021.10.033 【Objective】To investigate the effect of paclitaxel combined with cisplatin intraperitoneal thermal perfusion chemotherapy on tumor markers and prognosis in patients with ovarian cancer.【Methods】80 women diagnosed with ovarian cancer in our hospital were randomly divided into observation group and control group, with 40 cases in each group. The control group was treated with paclitaxel combined with cisplatin intravenous drip, and the observation group was treated with paclitaxel combined with cisplatin intraperitoneal circulation thermal perfusion. The curative effects of the two groups after 4 months of treatment were compared; The levels of serum carcinoembryonic antigen (CEA), alpha fetoprotein (AFP), neuron specific enolase (NSE), carbohydrate antigen 125 (CA125) and kappa functional state score (KPS) were evaluated and compared between the two groups before and after treatment; The long-term survival and adverse reactions of the two groups were compared.【Results】the objective remission rate (ORR) in the observation group was 80.00% (32/40), which was significantly higher than 57.50% (23/40) in the control group (P<0.05). CEA, NSE and CA125 in the observation group were significantly lower than those before treatment and after treatment in the control group, and KPS scores were significantly higher than those before treatment and after treatment in the control group (P<0.05). The 1-year survival rate in the observation group was significantly higher than that in the control group (P<0.05). The total incidence of adverse reactions in the observation group was 80.00% (32/40), and there was no significant difference compared with 67.50% (27/40) in the control group (P>0.05).【Conclusion】Paclitaxel combined with cisplatin for intraperitoneal circulation thermal perfusion is more effective and safe, and has a positive impact on improving the prognosis of patients. 2021 Vol. 38 (10): 1556-1559 [Abstract] ( 32 ) HTML (1 KB)  PDF (1177 KB)  ( 64 ) 1560 Significance of DKK-1, β-catenin and Caspase 3 Protein Expression in Patients with DLBCL DING Feng-Jiao, CAO Hong-you DOI: 10.3969/j.issn.1671-7171.2021.10.034 【Objective】To observe the expression of DKK-1, β-catenin and caspase 3 in patients with diffuse large B-cell lymphoma (DLBCL) and analyze the influencing factors. 【Methods】The patients with DLBCL who were treated in our hospital from February 2014 to August 2019were enrolled. The expressions of DKK-1, β-catenin and caspase 3 in cancer tissues and paracancerous tissues of patients with DLBCL were detected and their effects were analyzed. 【Results】The positive expression rates of DKK-1, β-catenin and caspase 3 in cancer tissues of DLBCL patients were significantly higher than those in cancer adjacent tissues. The difference was statistically significant (P<0.05). The clinical stage in Ⅲ+Ⅳ, PS score ≥2, and IDH increased. The positive expression rates of DKK-1, β-catenin and caspase 3 in DLBCL patients with clinical stage Ⅲ+Ⅳ, PS (performance status) score ≥2, high IDH (isocitrate dehydrogenase), and high risk of IPI (international prognostic index) were much higher (P<0.05). There were no significant differences in the positive expression rates of DKK-1, β-catenin and caspase 3 in patients with different ages and genders. DKK- patients with DLBCL 1. Multivariate logistica repression showed that clinical stage and IDH were the factors that affected the expression of DKK-1, β-catenin and caspase 3 expression in DLBCL patients (OR=4.865, 5.013). 【Conclusion】The positive expression rates of DKK-1, β-catenin and caspase 3 in patients with diffuse large B-cell lymphoma are high, which are affected by clinical stage and IDH. 2021 Vol. 38 (10): 1560-1562 [Abstract] ( 36 ) HTML (1 KB)  PDF (1160 KB)  ( 37 )

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