长非编码RNA HOTAIR在结直肠癌光动力治疗中的作用机制研究

doi:10.3969/j.issn.1671-7171.2021.10.003

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Abstract 【Objective】To explore the mechanism of long non-coding RNA(LncRNA)-HOX transcribed antisense RNA(HOTAIR) in photodynamic therapy (PDT) of colorectal cancer. 【Methods】The expression of lncrna hotair in colorectal cancer cells (CRCs) treated with PDT was detected by real-time fluorescence quantitative PCR (qPCR). CCK8 kit and plate clone formation experiment were used to detect the proliferation of CRCs after interfering with the expression of LncRNA HOTAIR and treated with PDT. Meanwhile, western blot assay (WB) was used to detect the expression of cell proliferation-related proteins p38 and p-p38.Then, the intersection of LncRNA HOTAIR downstream regulatory protein and PDT sequencing data was analyzed by Starbase database to obtain both the regulatory protein of LncRNA HOTAIR and the down-regulated protein after PDT treatment. Finally, after interfering with the expression of LncRNA HOTAIR and overexpressing ENPP5, the cell proliferation level was detected by CCK8 kit and plate cloning experiment, and the expression of p38 and P-38 protein were detected by WB experiment.【Results】After PDT treatment, the cell morphology of CRCs changed significantly, and the expression of LncRNA HOTAIR was significantly down regulated. Interference with LncRNA HOTAIR expression in CRCs can improve the sensitivity to PDT. LncRNA HOTAIR in CRCs can positively regulate ENPP5 expression. Interfering with the expression of LncRNA HOTAIR in CRCs can down regulate the expression of ENPP5, affect the p38 signal pathway and promote the sensitivity to PDT.【Conclusion】Interference with LncRNA HOTAIR expression increased CRCs sensitivity to PDT, and LncRNA HOTAIR positively regulated ENPP5 to affect PDT sensitivity to CRCs. It provides a new idea for improving the efficacy of PDT in colorectal cancer.

Key words： Colorectal Neoplasms/PA Photochemotherapy RNA,Long Noncoding

Received: 21 July 2021

PACS:

R735.37

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	Articles by authors
	GONG Hui
	LIAO Ping
	YANG Qing-long
	et al

Cite this article:

GONG Hui,LIAO Ping,YANG Qing-long, et al. Mechanism of LncRNA HOTAIR in Photodynamic Therapy of Colorectal Cancer[J]. JOURNAL OF CLINICAL RESEARCH, 2021, 38(10): 1448-1452.

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2021.10.003 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2021/V38/I10/1448

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