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| Effects of Acute Cerebral Infarction on the Levels of Dendritic Cells and Inflammatory Factors in Rats |
| ZHONG Zhen-zhen, XU Ping, WANG Kai, et al |
| First People's Hospital of Changde City,Changde 415000,China |
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Abstract 【Objective】 To to study the effects of acute cerebral infarction on the levels of dendritic cells (DCS) and inflammatory factors in rats.【Methods】 Twenty 8-week-old female SD rats were randomly divided into experimental group and control group, with 10 rats in each group. The middle cerebral artery occlusion (MACO) rat model was made by suture method. The operation steps of the control group were the same as those of the experimental group, but the suture was not inserted. Immunofluorescence, immunohistochemistry and ELISA were performed 7 days after operation. Expression levels of DCs and interleukin-1β (IL-1 β), Interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) in the experimental group and the control group were observed.【Results】 Compared with the control group, the quantity of positive cells of CD74 and integrinαE in the ischemic brain tissue of the experimental group was bigger, and the difference was statistically significant (P<0.05);The expression of IL-1β,IL-6 and TNF-α in ischemic brain tissue of rats in experimental group was significantly higher than that in the control group (P<0.05);The difference of the contents of serum IL-1β,IL-6 and TNF- α of the two groups was statistically significant (P<0.05). 【Conclusion】Cerebral infarction induces the expression of DCs and inflammatory factors in rats, and the inflammatory response is a pathological process of acute cerebral infarction.
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Received: 28 July 2021
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2021, Vol. 38 
