TLR4/NF-κB通路及相关细胞因子表达水平变化与急性胰腺炎病情发展的关系

doi:10.3969/j.issn.1671-7171.2022.12.023

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摘要【目的】探讨Toll样受体4(TLR4)/核因子κB(NF-κB)通路及相关细胞因子表达水平变化与急性胰腺炎(AP)病情发展的关系。【方法】将本院收治的127例AP患者依据病情严重程度分为AP组(75例)和重症急性胰腺炎(SAP)组(52例),并选择同一时间段在本院进行体检的80例健康志愿者作为对照组。比较三组对象的临床资料、TLR4、NF-κB表达水平及血清炎性因子水平。采用Pearson相关性分析TLR4/NF-κB通路及相关细胞因子表达水平变化与AP床旁严重度指数(BISAP)的关系。【结果】AP组和SAP组血清脂肪酶、淀粉酶均显著高于对照组(P<0.05)。AP组和SAP组TLR4 mRNA、NF-κB mRNA水平均高于对照组(P<0.05),且SAP组TLR4 mRNA、NF-κB mRNA均显著高AP组(P<0.05)。AP组和SAP组的白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)及白细胞介素-1β(IL-1β)水平均显著高于对照组(P<0.05);SAP组IL-6、TNF-α及IL-1β水平均显著高于AP组(P<0.05)。TLR4 mRNA、NF-κB mRNA、TNF-α及IL-1β水平与BISAP评分均呈正相关(P<0.05),IL-6水平与BISAP评分无相关性(P>0.05)。【结论】TLR4/NF-κB通路及相关细胞因子表达水平变化与AP病情发展具有一定的相关性,可作为临床评估AP疾病进展的参考依据。

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	王永锋
	安翠红
	徐焰

关键词 ：胰腺炎, 急性病, Toll样受体4, 信号传导

Abstract：【Objective】To explore the relationship of Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) pathway with related cytokine expression levels and the development of acute pancreatitis (AP). 【Methods】A total of 127 AP patients admitted to our hospital were divided into the AP group (75 cases) and the severe acute pancreatitis (SAP) group (52 cases) based on the severity of the illness. And 80 healthy volunteers who underwent physical examination in our hospital during the same time period were selected as the control group. The clinical data,TLR4,NF-κB expression levels and serum inflammatory factor levels were compared among the three groups. Pearson correlation was used to analyze the relationship of TLR4/NF-κB pathway with related cytokine expression levels and acute pancreatitis bedside severity index (BISAP). 【Results】The serum lipase and amylase of the AP group and SAP group were significantly higher than those of the control group (P<0.05). TLR4 mRNA and NF-κB mRNA in the AP group and the SAP group were higher than those in the control group (P<0.05). TLR4 mRNA and NF-κB mRNA in the SAP group were significantly higher than those in the AP group (P<0.05). The levels of interleukin-6 (IL-6),tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the AP group and SAP group were significantly higher than those in the control group (P<0.05). The levels of IL-6,TNF-α and IL-1β in the SAP group were significantly higher than those in the AP group (P<0.05). The levels of TLR4 mRNA,NF-κB mRNA,TNF-α and IL-1β were all positively correlated with BISAP (P<0.05),while there was no correlation between IL-6 levels and BISAP (P>0.05). 【Conclusion】The changes in TLR4/NF-κB pathway and related cytokine expression levels have a certain correlation with the development of AP disease,which can be a reference for clinical evaluation of AP disease progression.

Key words： Pancreatitis Acute Disease Toll-Like Receptor 4 Signal Transduction

收稿日期: 2022-03-15

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R576.1

通讯作者: * E-mail:183614223@qq.com

引用本文:

王永锋, 安翠红, 徐焰. TLR4/NF-κB通路及相关细胞因子表达水平变化与急性胰腺炎病情发展的关系[J]. 医学临床研究, 2022, 39(12): 1841-1843.
WANG Yong-feng, AN Cui-hong, XU Yan. Relationship of TLR4/NF-κB Pathway with Related Cytokine Expression and Development of Acute Pancreatitis. JOURNAL OF CLINICAL RESEARCH, 2022, 39(12): 1841-1843.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2022.12.023 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2022/V39/I12/1841

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