Abstract：【Objective】To investigate the correlation between the expression level of microRNA-27a (miR-27a) and the prognosis of Crohn's disease (CD). 【Methods】A retrospective analysis was conducted on the clinical data of 147 patients with CD admitted to our hospital from December 2019 to December 2021. The patients were divided into a good prognosis group (n=79) and a poor prognosis group (n=68) based on follow-up results. The receiver operating characteristic (ROC) curve was used to analyze the value of miR-27a in predicting poor prognosis in CD patients; unconditional logistic stepwise regression was used to analyze the risk factors for poor prognosis in CD patients. 【Results】The proportion of patients with age >40 years, disease type of stenosis and penetrating, and perianal lesions in the good prognosis group was lower than that in the poor prognosis group (P<0.05). The expression level of miR-27a after treatment in the good prognosis group was higher than that in the poor prognosis group (P<0.05). ROC curve analysis showed that the area under the curve for miR-27a expression level predicting poor prognosis in CD patients was 0.954 (95%CI 0.918-0.991), with an optimal cutoff value of 0.335, sensitivity of 0.911, and specificity of 0.941 (P<0.05). Multivariate logistic regression analysis showed that disease behavior of stenosis and penetrating, perianal lesions, and miR-27a ≤ 0.335 were risk factors for poor prognosis in CD patients (P<0.05). 【Conclusion】Stenosis and penetrating disease behavior, perianal lesions, and miR-27a ≤ 0.335 are risk factors for poor prognosis in CD patients. Close attention should be paid to such patients in clinical practice.
[1] YU M, ZHU W, WANG J, et al. Caveolin-1 alleviates crohn's disease-induced intestinal fibrosis by inhibiting fibroblasts autophagy through modulating sequestosome 1[J].Inflamm Bowel Dis,2022,28(6):923-935. [2] KIM H S, IN S K, KIM B S, et al. Chronic ulcer on flank region as first manifestation of crohn's disease[J].J Wound Manag Res,2020,16(2):117-120. [3] 胡菊香, 郑蓉, 周宇. 非编码RNA与炎症性肠病关系的研究进展[J].新医学, 2016, 47(7):425. [4] SARE V, GERT D H, JAN V D G, et al. Gene and mirna regulatory networks during different stages of crohn's disease[J].J Crohn's Colitis,2019,13(7):916-930. [5] ZHU Y, LIU S, WANG F . MicroRNA MiR-27a-5p alleviates the cerulein-induced cell apoptosis and inflammatory injury of AR42J cells by targeting Traf3 in acute pancreatitis[J].Inflammation,2020, 43(5):1988-1998. [6] 中华医学会病理学分会清化病理学组筹备组,中华医学会消化病学分会炎症性肠病学组.中国炎症性肠病组织病理诊断共识意见[J].中华病理学杂志,2014,43(4):268-274. [7] 王木岭,万苹,李娅琳,等.克罗恩病发病机制及其药物治疗的研究进展[J].中国医院药学杂志,2020,40(17):1891-1895. [8] 陈媛媛,魏娟,孙晓伟,等.5-氨基水杨酸和免疫抑制剂药物预防上消化道型克罗恩病复发的疗效与安全性分析[J].医学研究生学报,2021,34(3):252-257. [9] 沈佳佳,范一宏,吕宾,等.血清学标志物检测在克罗恩病分型管理中的价值[J].中华消化杂志,2018,38(1):14-19. [10] 黄超群,王德欣.结肠型与非结肠型克罗恩病的预后及预后不良相关危险因素分析[J].胃肠病学和肝病学杂志,2018,27(5):518-522. [11] 刘理静,钱红,胡柯,等.miR-27a-3p通过Wnt3a/β-catenin信号通路抑制肺成纤维细胞Ⅰ、Ⅲ型胶原合成[J].中国药理学通报,2019,35(2):229-234. [12] 李宁宁,戴冰冰,雷蕾,等.原发性痛风性关节炎患者miR-23a、miR-24-2和miR-27a的表达水平及调节机制[J].河北医学,2019,25(8):1233-1237.
2023, Vol. 40 
