Abstract：【Objective】To construct tumor necrosis factor alpha-induced protein 1 (TNFAIP1)-shRNA lentiviral vector.【Methods】Four shRNA sequences were designed and synthesized according to the sequence of human TNFAIP1 gene. These recombinant plasmids were transfected into 293T cells. RT-PCR assay was applied to detect the mRNA expression level of TNFAIP1 in each group and thus to choose the most effective TNFAIP1-shRNA. The HSH018143-LVRH1P-RNAi was constructed and confirmed by DNA sequencing. The selected TNFAIP1-shRNA-0S523409 was transfected into 293T cells. After 72 hours, the green fluorescence expression was observed and the lentiviral titer was tested.【Results】The TNFAIP1-shRNA lentiviral vector had been transfected into 293T cells efficiently and stably, the titer of lentivirus was 5×108 TU/mL.【Conclusion】The TNFAIP1-shRNA lentiviral expression vector has been constructed successfully, which lays a foundation for exploring for the role of TNFAIP1 in diabetic retinopathy.
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2018, Vol. 35 
