Abstract：【Objective】 To analyze the expression level of Cofilin-1 in glioma cell lines and explore its effects on proliferation, apoptosis and invasion of glioma cells. 【Methods】 Cofilin-1 overexpression plasmid was constructed and transfected into human glioma cell line U-87MG cells. The transfection efficiency of Cofilin-1 was detected by Real-time PCR and Western blot. The proliferation of U-87MG was assessed by MTT assay; Transwell assay was used to detect cell invasion; Contents of VEGF and MMP-9 in cell culture supernatant were measured by ELISA; Western blot was used to detect the protein levels of bax, Bcl-2, AKT, and p-AKT. 【Results】 Cofilin-1 overexpression plasmids were constructed by gene cloning technique and transfected into human glioma cell line U-87MG cell line. Real-time PCR and Western blot were used to test the effect of Cofilin-1 transfection. The levels of vascular endothelial growth factor (VEGFA) and matrix metalloproteinase-9 (MMP-9) in the supernatant of cultured cells were determined by ELISA assay. The expressions of bax, Bcl-2, AKT and p-AKT proteins were detected by Western blot. MTT assay was used to detect cell proliferation and transwell chamber assay was used to detect cell invasion ability.ELISA assay was used to detect vascular endothelial growth factor (VEGFA) and matrix metalloproteinase-9 (MMP-9) in supernatant of cell culture. The expression of apoptosis-related proteins (bax and Bcl-2, AKT and p-AKT) was detected by Western blot.【Conclusion】 Overexpression of Cofilin-1 can promote the proliferation and invasion of glioma cells. The mechanism may be that the activation of AKT pathway may play a role in Cofilin-1, which may become a potential therapeutic target for glioma.
赵云东, 孙冬弢, 陈振军. 过表达Cofilin-1对人胶质瘤细胞侵袭和增殖及凋亡的影响[J]. 医学临床研究, 2018, 35(9): 1665-1668.
ZHAO Yun-dong, SUN Dong-tao, CHEN Zhen-jun. Effect of Cofilin-1 Vverexpression on the Invasion, Proliferation and Apoptosis of Human Glioma Cells. JOURNAL OF CLINICAL RESEARCH, 2018, 35(9): 1665-1668.
[1] Stupp R, Brada M, van den Bent MJ,et al. High-grade glioma: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up[J].Ann Oncol,2014,25(Suppl 3): 93-101. [2] Ahmed R, Oborski MJ, Hwang M, et al. Malignant gliomas: current perspectives in diagnosis, treatment, and early response assessment using advanced quantitative imaging methods [J].Cancer Manag Res,2014,6:149-170. [3] Van Troys M, Huyck L, Leyman S, et al. Ins and outs of ADF/cofilin activity and regulation [J].Eur J Cell Biol,2008,87(8-9):649-667. [4] Wang F, Wu D, He F, et al. Study on the significance of Cofilin 1 overexpression in human bladder cancer [J].Tumori,2017,103(6):537-542. [5] Demuth T, Berens ME. Molecular mechanisms of glioma cell migration and invasion[J].J Neurooncol,2004,70(2):217-228. [6] Stupp R, Hegi ME, Mason WP, et al. Effects of radiotherapy with concomitant and adjuvant temozolomide versus radiotherapy alone on survival in glioblastoma in a randomised phase Ⅲ study: 5-year analysis of the EORTC-NCIC trial [J].Lancet Oncol,2009,10(5):459-466. [7] Sidani M, Wessels D, Mouneimne G, et al. Cofilin determines the migration behavior and turning frequency of metastatic cancer cells [J].J Cell Biol,2007,179(4):777-791. [8] Wang F, Wu D, Fu H, et al. Cofilin 1 promotes bladder cancer and is regulated by TCF7L2 [J].Oncotarget,2017,8(54):92043-92054.
2018, Vol. 35 
