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Quick Search     Adv Search 2017 Vol. 34, No. 7 Published: 2017-07-30 Article          Article 1249 Cytotoxicity of Oridonin on Human Bladder Cancer T24 and 5637 Cells SHI Hua-juan, ZHU Yun-guo, GENG He,et al DOI: 10.3969/j.issn.1671-7171.2017.07.001 【Objective】To investigate the the cytotoxicity of oridonin on different malignant types of human bladder cancer cells. 【Methods】With mitomycin as a control, different concentrations of oridonin were treated to human bladder cancer T24 (invasive, G3) and 5637 (non-invasive, G2) cells. Cell proliferation inhibition was measured by MTT assay and IC50 values were calculated for comparison. Cell morphologic changes and apoptosis were observed by inverted microscope and fluorescence staining. 【Results】Oridonin and mitomycin inhibited cell proliferation and killed both T24 and 5637 cells, both showing time- and concentration-dependent effects. The proliferation inhibition and killing effects of oridonin at 64μM were significantly better than those of mitomycin at 150μM after treating T24 and 5637 cells for 12 h and 24 h. Compared with highly malignant invasive bladder cancer cells T24, low malignancy non-invasive bladder cancer cells 5637 were more sensitive to oridonin and mitomycin treatment. 【Conclusion】 Compared with mitomycin, oridonin induces apoptosis, inhibits proliferation, and kills bladder cancer T24 and 5637 cells to a greater extend. It is worthy of further research and development. 2017 Vol. 34 (7): 1249-1253 [Abstract] ( 193 ) HTML (1 KB)  PDF  (0 KB)  ( 14 ) 1253 Streptozotocin-induced Diabetic Itching Model in C57BL/6 Mice PAN Bing-bing, XIAO Dan, HUANG Xiao-ling, et al DOI: 10.3969/j.issn.1671-7171.2017.07.002 【Objective】 To investigate the optimal dosage of STZ (streptozotocin) in inducing diabetic itch in C57BL/6 mice. 【Methods】Twenty-four male aged 8 weeks old C57BL/6 wild type mice (the weight 20~28 g) were randomly divided into 4 groups. A. Control group (mice were given the same dose solvent by intraperitoneal injection); B. High dose group (mice were intraperitoneally injected with STZ 160mg/kg); C. Low dose group (mice were intraperitoneally injected with STZ 40mg/kg 5 times continuously and the interval time was 24 hours); D. Mild moderate dose group (mice were intraperitoneally injected with STZ 85mg/kg + 65mg/kg and the interval time was 24 hours). Mice in the Group A were fed with normal food and B、C、D groups mice were fed with high-fat food from 4 weeks before the injection to the end of experiment. At the time of 1D before the injection and 1,2,3,4 weeks after injection, the fasting blood glucose, body weight and scrathing bouts in 30 minutes were detected. 【Results】Compared with the group A, the blood glucose level of other groups increased to the diabetic standard(＞200mg/dl)at different time point (P＜0.05). The weight of the group B was lower than that of the group A, group C and group D (P＜0.05), and the number of scratching in the group D was increased more than that of A, B and C at second, third weeks after STZ injection (P＜0.05).【Conclusions】It is a suitable way to establish the diabetic itch model of mice induced by intraperitoneal injection 85mg/kg+65mg/kg STZ combining with feeding high-fat food. 2017 Vol. 34 (7): 1253-1255 [Abstract] ( 105 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1256 Clinical Value of Dynamic Monitoring of Reticulocyte Count in the Evaluation of Bone Marrow Suppression Induced by Chemotherapy of Breast Cancer LI Sheng DOI: 10.3969/j.issn.1671-7171.2017.07.003 【Objective】To explore the value of dynamic monitoring of reticulocyte count in the evaluation of bone marrow suppression induced by chemotherapy on breast cancer. 【Methods】One hundred and ten patients with breast cancer received chemotherapy. 21d was a course of treatment, and the patients were intervened for 1 ~ 4 courses. The automatic blood cell analyzer was used to determine the percentages of reticulocyte, high fluorescencereticulocyte (HFR) and moderate fluorescencereticulocyte (MFR) before each course of chemotherapy, at 1 week, 2 weeks and 3 weeks after chemotherapy. 【Results】 Compared with those before chemotherapy, RET% and HFR at 1 week and 2 weeks after the 1st ~ 4th course of hcemotherapy were significantly lower, and the difference was significant (P＜0.05); At 3 weeks after the 1st ~ 3rd course of hcemotherapy, there were no significant differences in RET%, HFR and MFR, compared with those before chemotherapy (P＞0.05);There were no significant differences in MFR at 2 weeks after the 3rd ~ 4th course of chemotherapy, compared with that before chemotherapy (P＞0.05); At 3 weeks after the 4th course of chemotherapy, HFR was significantly lower than that before chemotherapy (P＜0.05). 【Conclusion】The dynamic monitoring of reticulocyte related indexes during different periods of chemotherapy of breast cancer plays an important role in the evaluation of bone marrow suppression and recovery. 2017 Vol. 34 (7): 1256-1258,1260 [Abstract] ( 79 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 1258 Comparison of Prognosis of Patients with Liver Metastases of Colorectal Cancer Treated by Microwave Ablation, Transhepatic Arterial Chemotherapy and Embolization and Systemic Chemotherapy AI Dong-mei, QIAO Xiu-zhen, YU Hong-wei,et al DOI: 10.3969/j.issn.1671-7171.2017.07.004 【Objective】To compare the prognosis of patients with liver metastases of colorectal cancer treated by microwave ablation, transhepatic arterial chemotherapy and embolization (TACE) and systemic chemotherapy. 【Methods】According to different treatment methods, 80 cases of patients with liver metastases of colorectal cancer were divided into the observation group (treated by microwave ablation+TACE sequential therapy) and the control group (treated by systemic chemotherapy). The short-term curative effect and prognosis (survival) were compared between the two groups, and the changes of cell immune function before and after treatment were determined and recorded. 【Results】The short-term objective response rate (ORR) of the observation group was 66.67% (28/42)while that of the control group was 42.10%(16/38), and the difference was significant (P＜0.05). After initial treatment, positive rate of CD3+, CD4+ and the ratio CD4+/CD8+ were significantly higher, and CD8+ was significantly lower in the observation group than those in the same group before treatment and those in the control group after treatment (P＜0.05). The median survival time of the observation group was 26.00 months while that of the control group was 13.60 months (P＜0.05). 【Conclusion】On the basis of systemic chemotherapy , microwave ablation + TACE can significantly improve the effective rate of treatment for patients with liver metastases of colorectal cancer, significantly improve the immune function and prolong the survival time of patients. 2017 Vol. 34 (7): 1258-1260 [Abstract] ( 92 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 1261 Clinical Features and Prognosis of Scar Uterus Re-pregnancy Complicated with Placenta Previa WANG Rui-xia, WEI Ling, HE Jian-hua DOI: 10.3969/j.issn.1671-7171.2017.07.005 【Objective】To analyze the clinical features, mother and infant outcomes of scar uterus re-pregnancy complicated with placenta previa, and to explore the intervention measures. 【Methods】 A total of 174 cases with placenta previa treated in our hospital from March 2015 to March 2017 were analyzed retrospectively. According to the existence of maternal uterine scar, the patients were included in the uterine scar group (study group,n=81) and non scar uterus group (control group,n=93). The clinical characteristics and maternal outcome, the impact of uterine scar pregnancy complicated with placenta previa on maternal and neonatal outcomes in each group were analyzed. 【Results】 There was no significant difference between the two groups in the general clinical data such as the age of the pregnant women, the gestational age of admission, the type of placenta previa and the location of placenta previa (P＞0.05). The pregnancy rate of observation group was higher than that of control group, the difference was statistically significant (P＜0.05). The incidence of painless vaginal bleeding and the average amount of vaginal bleeding before operation in two groups were not statistically significant (P＞0.05). Two groups of pregnant women were terminated by caesarean section. The operation time and the amount of postpartum hemorrhage in the observation group were higher than those in the control group. The rate of intraoperative blood transfusion, the rate of intrauterine gauze filling, the rate of placental attachment, the rate of placenta accreta, the rate of suture and the rate of hysterectomy were also higher than those of the control group, the difference was statistically significant (P＜0.05); Premature birth rate of newborn, asphyxia rate and mortality rate of the observation group were slightly higher than that of the control group, but there was no significant difference between the two groups (P＞0.05).【Conclusion】The clinical features of scarred uterus secondary to placenta previa are similar to those of simple placenta previa, but its effect on the outcome of the m aternal is more severe than that of the simple placenta previa. Therefore, attention should be payed to active intervention to ensure the safety of mother and baby. 2017 Vol. 34 (7): 1261-1264,1266 [Abstract] ( 95 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 1264 Effect of Central Venous Catheter Disinfection Methods and Catheterization on the Incidence of Catheter-related Infections of Hemodialysis Patients GUO Hai-li DOI: 10.3969/j.issn.1671-7171.2017.07.006 【Objective】 To investigate the application value of an improved disinfection method of central venous catheter on the prevention and reduction of catheter-related infections of hemodialysis patients. 【Methods】 A total of 200 cases of patients received central venous catheter and hemodialysis in our hospital from January 2016 to January 2017; they were selected as the research objects and were divided into the conventional disinfection method (Group A) and improved disinfection method (Group B) using the single blind method and random number table method, with 100 cases in each group. The catheter retention, the incidence of catheter-related infection (CRI), and the catheter local infection rate at different catheterization sites were compared between the two groups. 【Results】 The retention time of catheter in Group B was (32.96 ± 14.51) d and was significantly longer than that in Group A (25.38 ± 10.19) d. The catheter local infection rate in Group B was 6.00 % significantly lower than that of 17.00 % in Group A (P＜0.05). The incidence of CRI in Group A and Group B was 5.00% and 2.00%, respectively, and the difference was not statistically significant (P＞0.05). The infection rate of internal jugular vein and the rate of femoral vein in Group B were significantly lower than those in Group A (P＜0.05). 【Conclusion】 the improved disinfection method is conducive to prolonging the retention time of catheters and reducing the catheter local infection rate in hemodialysis patients, so it is suggested to be popularized and applied in clinical practice. 2017 Vol. 34 (7): 1264-1266 [Abstract] ( 85 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1267 Finite Element Analysis of Improved Anatomical Titanium Mesh Cage in ACCF Surgery OUYANG Zhi-hua SUN Jian-te, WANG Cheng,et al DOI: 10.3969/j.issn.1671-7171.2017.07.007 【Objective】To evaluate biomechanical property of improved anatomical titanium mesh cage (IMTMC) in anterior cervical corpectomy and fusion (ACCF).【Methods】A finite element model of intact cervical spine (C3~7) was developed to simulate the ACCF operation process, establishing both traditional titanium mesh cage and improved anatomical titanium cage. Tests were made with pre-load of 40N and 1.5Nm torque to the model, which caused motion of flexion, extension, left, right bending and left, right rotation of the model. The maximum stress, stress distribution of both two kinds of cages, cervical vertebra and TMC-endplate contact were recorded.【Results】Under 6 different conditions of flexion, extension, left, right, left turn, right turn , the maximum equivalent stress values of the traditional cervical titanium mesh were 73.782, 177.36, 124.42, 100.85, 191.64 and 221.91 MPa, respectively; the maximum equivalent stress values of the new modified titanium mesh were 31.028, 62.236, 46.053, 44.337, 41.549 and 39.255 MPa, respectively; The stress distribution of the vertebral body of the new modified titanium mesh was much smaller than that of the traditional titanium mesh; The stress of C4 and C6 endplate contact surface of the modified titanium mesh was less than that of traditional titanium mesh contact point.【Conclusion】The application of modified anatomic cervical titanium mesh in ACCF can effectively avoid the stress concentration of endplate, and can reduce the incidence of titanium mesh depression theoretically. 2017 Vol. 34 (7): 1267-1270 [Abstract] ( 96 ) HTML (1 KB)  PDF  (0 KB)  ( 14 ) 1271 Study of Autophagy and Expression of Ubiquitin-related Proteins on Lipid-Storage Myopathy ZHU Yong, BI Fang-fang, LONG Fen,et al DOI: 10.3969/j.issn.1671-7171.2017.07.008 【Objective】 To investigate the effects of autophagy and expression of ubiquitin-related proteins on lipid storage myopathy (LSM). 【Methods】Thirty patients with lipid deposition myopathy who were treated in our hospital from January 2010 to December 2015 were selected as the study subject pool, and 15 cases were selected as the observation group by random data table method. In the same period, 15 healthy subjects who matched the observation group patients in age and sex were selected as the control group. Western blot was used to investigate the expression of autophagy and ubiquitin-related proteins in skeletal muscle tissue. 【Results】 The expression of autophagy-related proteins p62 and LC3 Ⅰ (microtubule-associated protein Ⅰ light chain 3 ) in the skeletal muscle of the observation group was significantly higher than that of the control group, while the expression level of LC3 Ⅱ was significantly lower than that of the control group; the ratio of LC3 Ⅰ to LC3 Ⅱ in the observation group was significantly higher than that of the control group. In addition, the expression of MuRF1, Astrogin-1 and ZNF216 in the skeletal muscle of the observation group was significantly higher than those in the control group (P＜0.05), and the difference was statistically significant (P＜0.05). 【Conclusion】The inhibition of autophagy and the increased expression of ubiquitination-related proteins are involved in the development of lipid-induced myopathy. 2017 Vol. 34 (7): 1271-1274,1276 [Abstract] ( 58 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 1274 Analysis of Related Factors of Cholecystitis after Radical Gastrectomy for Gastric Cancer WANG Ling, WU Shao-feng, FAN De-qing, et al DOI: 10.3969/j.issn.1671-7171.2017.07.009 【Objective】To investigate the influencing factors of postoperative cholecystitis in patients undergoing radical gastrectomy for gastric cancer. 【Methods】Clinical data of 180 cases of radical gastrectomy for gastric cancer treated in our hospital from May 2011 to April 2016 were selected. The selected subjects were followed up and divided into observation group 1 month after operation (n=59, cholecystitis complicated) and control group (n=121, without cholecystitis after operation complication). The clinical data of the two groups were compared and analyzed. The Logistic regression analysis of single factor with statistical difference was carried out.【Results】The difference in the fasting blood glucose abnormalities, the choice of surgical methods, whether No.8a lymph node dissection and No.12 lymph node dissection were compared between the observation group and the control group, the difference was statistically significant (P＜0.05).The abnormality of fasting blood glucose, the choice of operation mode, whether No.8a lymph node dissection and No.12 lymph node dissection were the independent risk factors for postoperative cholecystitis in patients with gastric cancer (P＜0.05).【Conclusion】Patients with preoperative fasting glucose abnormalities or those requiring total gastrectomy should be closely observed for postoperative cholecystitis. For patients without these conditions, care must be taken to protect the associated nerve during surgical dissection of the lymph nodes so to reduce injury as much as possible and maintain the normal function of the gallbladder after surgery, thus effectively reduce the incidence of postoperative cholecystitis. 2017 Vol. 34 (7): 1274-1276 [Abstract] ( 114 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1277 Effect of Arsenic Trioxide on Proliferation and Extracellular Matrix Metabolism of Hypertrophic Scar Fibroblasts ZHANG Qin-xue DOI: 10.3969/j.issn.1671-7171.2017.07.010 【Objective】To explore effect of arsenic trioxide (ATO) on proliferation and extracellular matrix metabolism of hypertrophic scar fibroblasts (HSF). 【Methods】HSF viability was measured by MTT assay after cell was cultured with 0, 2, 4, 8 μmol/L dose of ATO. Cell apoptosis and cell cycle was measured by flow cytometry. The level of CoLⅠ and CoL Ⅲ was detected by enzyme linked immunosorbent assay (ELISA). The expression of matrix metalloproteinase-1 (MMP-1) and MMP-13, and tissue inhibitors of matrix metalloproteinases-1 (TIMP-1) was detected by western blot. 【Results】Compared to the no-added- ATO control group, 2, 4, 8 μmol/L dose of ATO reduced cell viability (P＜0.01), increased cell early apoptotic rate and late apoptotic rate (P＜0.01), made cell cycle arrested in the G1 phase (P＜0.01), reduced the level of CoLⅠ and CoL Ⅲ (P＜0.01), down-regulated the expression of TIMP-1 (P＜0.01) and up-regualted the expression of MMP-1, MMP-13 (P＜0.01). Compared to 2 μmol/L, 4and 8 μmol/L doses of ATO reduced cell viability (P＜0.01), increased cell early apoptotic rate and late apoptotic rate (P＜0.01), made cell cycle arrested in the G1 phase (P＜0.01), reduced the level of CoLⅠ and CoL Ⅲ (P＜0.01), down-regulated the expression of TIMP-1 (P＜0.01) and up-regualted the expression of MMP-1, MMP-13 (P＜0.01). Compared to 4 μmol/L, 8 μmol/L dose of ATO reduced cell viability (P＜0.01), increased cell early apoptotic rate and late apoptotic rate (P＜0.01), made cell cycle arrested in the G1 phase (P＜0.01), reduced the level of CoLⅠ and CoL Ⅲ (P＜0.01), down-regulated the expression of TIMP-1 (P＜0.01) and up-regualted the expression of MMP-1, MMP-13 (P＜0.01). 【Conclusion】The results showed that ATO significantly inhibited the proliferation of HSF, reduced the synthesis of extracellular matrix, and promoted the degradation of extracellular matrix. 2017 Vol. 34 (7): 1277-1280 [Abstract] ( 97 ) HTML (1 KB)  PDF  (0 KB)  ( 14 ) 1281 Jinwu Gutong Capsule Combined with Sodium Hyaluronate in the Treatment of Elderly Patients with Osteoarthritis DUAN Xian-bin, LU Xiao-hu, PEI Zhi-jie DOI: 10.3969/j.issn.1671-7171.2017.07.011 【Objective】To investigate the curative effect of Jinwu Gutong Capsule combined with sodium hyaluronate in the treatment of elderly patients with knee osteoarthritis . 【Methods】Ninety-two elderly patients with knee osteoarthritis were randomly divided into observation group (n=46) and control group (n=46). The control group was treated with injection of sodium hyaluronate; on the basis of the control group, the observation group was treaded with Jinwu Gutong Capsule . The course of treatment was 4 weeks in two groups. The therapeutic effects of the two groups were compared before and after treatment. The curative effect, the severity score before and after treatment, the knee function score, the changes of serum IL-1 and IL-6 levels, and the adverse reactions were compared between the two groups. 【Results】The total efficiency of the observation group 91.31%(42/46) was higher than that in the control group69.57% (32/46), the difference was statistically significant (P＜0.05); The scores of severity, serum IL-1 and IL-6 in the observation group were significantly lower than those in the control group, and the difference was statistically significant (P＜0.05); The knee function score of the observation group was significantly higher than that of the control group, the difference was statistically significant (P＜0.05); ECG, blood routine, urine routine, liver and kidney function of the two groups were not abnormal.【Conclusion】 Jinwu Gutong Capsule combined with sodium hyaluronate in the treatment of elderly patients with knee osteoarthritis can improve the knee joint function of patients, reduce the cytokines IL-1 and IL-6 levels ,and it is worthy of clinical application. 2017 Vol. 34 (7): 1281-1284,1287 [Abstract] ( 71 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 1284 Investigation and Analysis of the Influencing Factors for Delivery Mode Selection in Pregnant Women with Scarred Uterus after Cesarean Section XIONG Hai-yan, ZHONG Min, FU Zhao-xia,et al DOI: 10.3969/j.issn.1671-7171.2017.07.012 【Objective】To understand the influencing factors of delivery mode selection in pregnant women with scarred uterus after cesarean section. 【Methods】The pregnant women with scarred uterus after cesarean section and their family members, and medical staff in the Department of Obstetrics and Gynecology from 10 hospitals in Shenzhen were selected as the research subjects during January to December,2016. The awareness of delivery modes ( natural labor and cesarean section) for pregnant women with scarred uterus and their family members and the knowledge of vaginal birth after cesarean section(VBAC)for medical staff were surveyed by questionnaire. The influencing factors of delivery mode selection for pregnant women with scarred uterus were analyzed by Logistic regression . 【Results】Among 993 pregnant women who chose VBAC for baby delivery, 648 cases were successful(65.25%). It was obviously much higher than pregnant women with repeated cesarean section(11.01%,χ2=31.22,P=0.000).Many factors such as living place of pregnant women, awareness of natural labor and cesarean section,tension about natural labor,previous history of vaginal delivery, and family members' suggestion for vaginal delivery had influence on the delivery mode selection(all P＜0.05).In the 738 effective questionnaires, the awareness rate of vaginal delivery by doctors was 61.65% (328/532), which was significantly higher than that of nurses (21.19%, 50/236). The difference was statistically significant (χ2=93.37,P=0.000).The awareness degree of VBAC by medical staff from tertiary hospitals was 68.46%,which was obviously higher than those from secondary (46.62%) and primary hospitals (20.69%); the difference was statistically significant(χ2=45.26,P=0.000).Furthermore, the VBAC awareness degree of medical staff in specialized hospitals was higher than that of general hospitals(P＜0.05);and the awareness degree of medical staff with senior professional title was higher than those of medical staff with junior and intermediate titles(P=0.000). 【Conclusion】The delivery mode selection of pregnant women with scarred uterus after cesarean section is made by the awareness of vaginal birth after cesarean section. It is necessary to strengthen the publicity of this knowledge. 2017 Vol. 34 (7): 1284-1287 [Abstract] ( 53 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1288 Study on the Relationship Between Serum 25-hydroxyvitamin D in Pregnant Women in the Second or Third-trimester of Pregnancy and Pregnancy Outcomes GAO Jing-jing, ZHOU Xiao-ping, ZHAI Jin-ya, et al DOI: 10.3969/j.issn.1671-7171.2017.07.013 【Objective】To investigate the relationship between serum 25-hydroxyvitamin D3in pregnant women in the second or third-trimester of pregnancy and pregnancy outcomes. 【Methods】The clinical data of 124 pregnant women in the second or third-trimester of pregnancy who received antenatal care in our hospital between February 2014 and January 2015 were analyzed retrospectively. According to the levels of serum 25 (OH)D3, the pregnant women were divided into the deficiency group [25 (OH) D3 level＜25 nmol/L,n=42], insufficiency group (25 nmol/L＜ 25 (OH) D3 level ＜ 50 nmol/L,n=52) and sufficiency group (25 (OH) D3 level ＞75 nmol/L,n=30). The adverse pregnancy outcomes and complications were compared between groups. 【Results】The incidence rates of abortion, preterm labor, preeclampsia, bacterial vaginosis and anemia in the deficiency group (35.71%, 47.62%, 30.92%, 38.1%, 28.57%) were significantly higher than those in the insufficiency group (15.38%, 26.92%, 19.23%, 6.67%, 17.31%) and the sufficiency group (6.67%,16.67%, 3.33%, 13.33%, 25.0%)(P＜0.05). The delivery cycle of the deficiency group [(37.12±0.52) weeks] was significantly shorter than that of the insufficiency group [(38.24±1.02) weeks] and the sufficiency group [(39.57±1.45) weeks](P＜0.05). 【Conclusion】Deficiency or insufficiency of serum 25-hydroxyvitamin D3 in pregnant women in the second or third trimester of pregnancy can increase the risk of adverse pregnancy outcomes and the incidence of pregnancy complications, and it can lead to fetal growth retardation. 2017 Vol. 34 (7): 1288-1290 [Abstract] ( 52 ) HTML (1 KB)  PDF  (0 KB)  ( 9 ) 1291 Analysis of Safety and Efficacy on Transforaminal Posterolateral Endoscopic Discectomy in Teenagers with Lumbar Intervertebral Disc Herniation GAO Xiao-liang, YANG Xiao-hui, HUANG Wei-min,et al DOI: 10.3969/j.issn.1671-7171.2017.07.014 【Objective】To explore the safety and efficacy of the Transforaminal Posterolateral Endoscopic Discectomy in teenagers with lumbar intervertebral disc herniation. 【Methods】A total of 72 teenagers with lumbar disc herniation were treated in our hospital from May 2013 to May 2015. According to the random number table method, patients were randomly divided into an observation group (36 cases) and a control group (36 cases). The control group was treated with conventional surgery while the observation group was treated with the transforaminal Posterolateral Endoscopic Discectomy 【Results】 The total efficiency of the observation group was 86.11% which was higher than that of the control group (63.89%) (P＜0.05). The length of the incision, operation time, hospitalization time, and postoperative ambulation time in the observation group were shorter than those in the control group; the differences were statistically significant (P＜0.05). the VAS scores of the two groups decreased 3 days, 1 months, 6 months and 12 months post-surgery (P＜0.05).Furthermore, the VAS scores in the observation group at above time points were lower than those in the control group (P＜0.05). On the other hand, the JOA scores in two groups had opposite results in the same period as above: both increased, and the control group showed higher scores than the observation group (P＜0.05). 【Conclusion】The transforaminal posterolateral endoscopic discectomy for teenagers with lumbar intervertebral disc herniation was effective and safe, and it has important research significance. 2017 Vol. 34 (7): 1291-1293 [Abstract] ( 91 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 1294 Correlation Analysis of Early Renal Injury with HIF-1α, Cys C, and β2-MG Levels in COPD Patients CHEN Yan,JIA Qi DOI: 10.3969/j.issn.1671-7171.2017.07.015 【Objective】The aim was to investigate the correlation among serum factors such as hypoxia-inducible factor-1α (HIF-1α), cystatin C (Cys C) and β2-microglobulin (β2-MG) with early renal injury in elderly patients with chronic obstructive pulmonary disease (COPD). 【Methods】A total of 95 elderly patients with COPD in our hospital from January 2012 to January 2016 were enrolled. They were divided into three groups:mild, moderate and severe hypoxia (A1, A2, and A3) according to oxygen partial pressure (PaO2). Based on pulmonary artery systolic pressure, patients were split into two groups B1 (without pulmonary hypertension) and B2 (pulmonary hypertension) . Thirty cases of healthy persons in the same period were selected as the control group. HIF-1α, Cys C, and β2-MG levels in serum were detected by ELISA. The renal function index (SCr, BUN,RBP and eGFR) were measured as well. The changes of the indexes were compared and analyzed. 【Results】The levels of HIF-1α, Cys C and β2-MG in serum were higher in COPD group than that in healthy control group (P＜0.05); eGFR was lower than that of the control group (P＜0.05). SCr, BUN and RBP had no significant difference between COPD group and control group. The levels of HIF-1α, Cys C and β2-MG in the control group were significantly lower than those in the A1 group, while those in the A1 group were lower than those in the A2 group (P＜0.05) and those in the A2 group were lower than those in the A3 group. The eGFR in the A2 and A3 groups were lower than that in the control group (P＜0.05). There was no significant difference in eGFR between the A1 group and the control group (P＞0.05). The levels of HIF-1α, Cys C and β2-MG were in order of control group ＜ group B1＜ group B2 (P＜0.05), and eGFR in the B2 group was lower than in the control group (P＜0.05). HIF-1α, β2-MG and Cys C were positively correlated (r= 0.563,r=0.425, P＜0.05), HIF-1α, Cys C, β2-MG and eGFR were negatively correlated (r=-0.611,r=-0.569,r=-0.482,P＜0.05). 【Conclusion】The results show that chronic hypoxia in COPD can cause early renal injury. Monitoring HIF-1α, Cys C and β2-MG levels in serum can be used to evaluate early renal injury in COPD patients. Combined detection may be important for early renal injury assessment in COPD patients. 2017 Vol. 34 (7): 1294-1297,1300 [Abstract] ( 48 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1297 Infection of Human Papillomavirus in Cervical Cancer Patients ZHENG Hai-xia, CHEN Zhong-dong, YANG Jie DOI: 10.3969/j.issn.1671-7171.2017.07.016 【Objective】To study the distribution of human papillomavirus (HPV) infection in patients with cervical cancer. 【Methods】A total of 346 cervical cancer patients from January 2012 to July 2015 in The First Affiliated Hospital of University of South China were collected for the study. Luminex 200 multifunction flow lattice analyzer (made in the United States) were used to detect HPV-DNA. The data were compared, analyzed ,and summarized. 【Results】The positive rate of HPV infection in 346 cases of cervical cancer was 80.35% (278/346), and HR-HPV infection accounted for 79.77% (276/346). The main HPV infection pattern was a single type infection (91.73%), and HPV type 16 was the most common HPV type,( 70.20%), followed by type 18 (10.98%), type 58 (5.10%),type 59 and 52 (3.14%),type 33(1.96%)、type 39 and 45(1.57%)、type 31(1.18%). The ages of cervical cancer patients with different HPV infection were different, but the incidence of HPV infection rate in each age groups was not statistically significant. Among the 346 cases of cervical cancer, 324 cases showed clear histological classification, including 289 cases of cervical squamous cell carcinoma (89.20%, 289/324), 28 cases of adenocarcinoma (8.64%, 28/324), and 7 cases of adenosquamous carcinoma (2.16%, 7/324). Squamous cell carcinoma is the most common, followed by adenocarcinoma and adenosquamous carcinoma. There were no significant difference in HPV infection rate among different histopathological types (P＞0.05). Cervical cancer with different histological diagnosis mainly showed one type of HPV infection (HPV infection rates in squamous cell carcinoma, adenocarcinoma and adenosquamous carcinoma were 74.05%, 71.43%,and 71.43%,irrespectively. HPV negative patients above were 52, 8 and 2 cases, irrespectively). 【Conclusion】HPV-16 is the most common HPV infection type, followed by HPV-18,58,59 and 52,33,39 and 45,and 31. Accordingly, vaccines including HPV type 16 combined with HPV type 18, 58, 59,52,33,39, 45,and 31 are necessary to prevent HPV infection. HPV-16 is mainly associated with cervical squamous cell carcinoma, while HPV-18 is mainly associated with adenocarcinoma and adenosquamous carcinoma. 2017 Vol. 34 (7): 1297-1299 [Abstract] ( 52 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 1301 Correlation between Helicobacter Pylori Infection and Liver Metastases of Gastric Cancer WANG Zi-hong DOI: 10.3969/j.issn.1671-7171.2017.07.017 【Objective】 To investigate the correlation between Helicobacter pylori (Hp) infection and liver metastases of gastric cancer. 【Methods】A total of 120 patients with gastric cancer were divided according to the presence of metastases into the liver metastasis group (n=52) and the non-liver metastasis group (n=68). The risk factors of clinical data from the two groups were analyzed by univariate analysis. Factors related to liver metastases of gastric cancer were analyzed by Logistic regression analysis. Based on the results of 14C urea breath test, the patients with liver metastases of gastric cancer were further divided into Hp positive sub-group and Hp negative sub-group, and the survival curves were compared between the two sub-groups. 【Results】There were significant differences between the liver metastasis and non-liver metastasis groups in terms of tumor stage, lymph node metastasis, depth of primary tumor invasion, vascular invasion, tumor size, Borrmann type and Hp infection (P＜0.05). Logistic multivariate regression analysis showed that tumor stage, lymph node metastasis, depth of primary tumor invasion and Hp infection were independent risk factors for liver metastases of gastric cancer. The mean survival time of Hp negative group was significantly longer than that of Hp positive group (P＜0.05). 【Conclusion】Lymph node metastasis, vascular invasion and Hp infection are the independent risk factors of liver metastases of gastric cancer, and Hp infection can affect the progression-free survival time of patients with liver metastases of gastric cancer. 2017 Vol. 34 (7): 1301-1303 [Abstract] ( 61 ) HTML (1 KB)  PDF  (0 KB)  ( 14 ) 1304 Effect of Propofol Combined with Remifentanil Intravenous Anesthesia on Preoperative Signs and Related Factors in Patients with Liver Cancer during Radiofrequency Ablation XIAN Lian-yan, ZHAO Jing DOI: 10.3969/j.issn.1671-7171.2017.07.018 【Objective】To investigate the effect of propofol combined with remifentanil intravenous anesthesia on signs, serum high-mobility group protein B1 (HMGB1) and cytokines in patients with liver cancer during radiofrequency ablation (RFA). 【Methods】A total of 76 patients with liver cancer treated with RFA in our hospital were divided into the remifentanil combined with propofol group (A group) and fentanyl combined with propofol group (B group) by random, with 38 cases in each group. The recovery time, VAS pain score and incidence rate of intraoperative complications were compared between the two groups. The changes of HR, MAP and SpO2 before operation (T 0), at 5 min after the beginning of RFA (T 1 ), immediately at the end of RFA (T 2 ) and at recovery (T 3 ) were recorded. The tumor necrosis factor α (TNF- α), interleukin -6 (IL-6) and HMGB1were compared before and after operation. 【Results】The recovery time of A group was shorter than B group, and the VAS pain score was lower than B group (P＜0.05). There was no significant difference in SpO2 between the two groups at different time points (P＞0.05). Compared to T 0 , MAP of the two groups was lower at T 1 , and the MAP of A group was higher than B group. From T 1～2 , HR of the two groups decreased, and HR of A group was lower than B group (P＜0.05). There was no significant difference at other time points (P＞0.05). TNF-α, IL-6 and HMGB1 were lower in A group than B group (P ＜ 0.05). There was no significant difference in the incidence of complications between the two groups (P＞0.05). 【Conclusion】Propofol combined with remifentanil intravenous anesthesia is effective in the anesthesia of RFA for liver cancer. It can better regulate inflammatory factors, although the pseudoalveolus respiratory monitoring is needed during operation. 2017 Vol. 34 (7): 1304-1306 [Abstract] ( 76 ) HTML (1 KB)  PDF  (0 KB)  ( 14 ) 1307 Effects of Dexmedetomidine Combined with Flurbiprofen Axetil on Stress Factors and Blood Glucose in Severe Burn Patients during General Anesthesia Recovery Period HUAI Qiao, YANG Yan-chao, GUO Hui-yi,et al DOI: 10.3969/j.issn.1671-7171.2017.07.019 【Objective】 To investigate the effects of dexmedetomidine combined with flurbiprofen axetil on stress factors and blood glucose in severe burn patients during general anesthesia recovery period. 【Methods】 A total of 80 cases of severe burn patients from June 2015 to January 2017 in our hospital were selected as the research subjects. According to the random number method, patients were divided into the dexmedetomidine group (DEX), flurbiprofen group ( FLU), dexmedetomidine combined flurbiprofen group (DEX + FLU), and the control group (C group). 30 min before the end of surgery, patients in the DEX group received an intravenous injection of dexmedetomidine (0.25 g/kg); the FLU group received an intravenous injection of flurbiprofen (50 mg); theDEX+FLU group received an intravenous injection of both dexmedetomidine (0.25 g/kg) and flurbiprofen (50 mg); and group C received the same amount volume as group DEX by intravenous injection. The levels of plasma epinephrine (E), norepinephrine (NE), cortisol (COR), and blood glucose (GLU) of all four groups were recorded before the induction of anesthesia (T 0), 15min before extubation (T 1), immediately after extubation (T 2), and 15min after extubation (T 3). Patients' recovery time, extubation time and incidence of restlessness during recovery were monitored as well. 【Results】Compared with T0, the levels of plasma E,NE ,COR, and GLU of the C, DEX, and FLU groups during T 1 to T 3 , were significantly higher; the difference was statistically significant (P＜0.05). Compared with group Cplasma E, NE, COR and GLU levels were significantly reduced in group DEX, FLU and DEX+FLU from T 1 ~T 3 ; and DEX+FLU group decreased the most (P＜0.05). The incidence of agitation in the DEX group and DEX+FLU group was significantly lower than that of C group; the difference was statistically significant (P＜0.05). The incidence of agitation in the FLU group was lower than that in the C group, but there was no significant difference (P＞0.05). 【Conclusion】 Dexmedetomidine combined with flurbiprofen can significantly reduce stress response and blood glucose levels in patients with severe burn during recovery period of general anesthesia and reduce the incidence of emergence agitationwithout prolonging the recovery time and extubation time. 2017 Vol. 34 (7): 1307-1310,1312 [Abstract] ( 69 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1310 Effect of Propofol Applied in LC on Cardiac Function, IR and Levels of Inflammatory Factors in Patients of Cholecystitis Complicated with T2DM YANG Hong-shen, CHEN Wei, SHU Yao DOI: 10.3969/j.issn.1671-7171.2017.07.020 【Objective】To explore the effect of propofol applied in LC on cardiac function, IR and levels of inflammatory factors in patients of cholecystitis complicated with T2DM. 【Methods】One hundred and four patients of cholecystitis complicated with T2DM treated by LC were randomized into study group(A) and control group(B), 52 in each group. The group A was treated with propofol while the group B was treated with isoflurane maintenance anesthesia. The changes of hemodynamic parameters MAP, HR were compared between the two groups before anesthesia (T 0), after anesthesia (T 1), 5min after establishment of pneumoperitoneum (T 2) and immediate after the surgery (T 3). The differences in IR index, inflammatory factors TNF-α, CRP and myocardial function indexes CK-MB, cTnI before and after treatment were recorded, and postoperative recovery was analyzed.【Results】① At T 1 , MAP and HR of both groups were significantly lower than those at T 0 (P＜0.05) but there were no significant differences between groups at different time points (P＞0.05). ②After surgery, IR and levels of TNF-α, CRP were significantly higher than those before operation, and those in the group A were higher than those of the group B (P＜0.05). There were no significant changes of CK-MB and cTnI in group A, compared with those before operation (P＞0.05) while those in group B were significantly higher than those before operation (P＜0.05). ③The time of spontaneous breathing recovery, duration of eye opening, extubation time and time of directive force recovery in the group A were significantly shorter than those of the group B (P＜0.05). 【Conclusion】 The application of propofol combined with fentanyl anesthesia in LC of patients with cholecystitis complicated with T2DM mellitus is of positive significance in improving the state of IR, regulating the surgical trauma stress response and protecting cardiovascular system. 2017 Vol. 34 (7): 1310-1312 [Abstract] ( 50 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1313 Effect of Subclinical Hypothyroidism with Gestational Hypertension on Maternal and Neonatal Outcomes CHEN Xiao-ling DOI: 10.3969/j.issn.1671-7171.2017.07.021 【Objective】To investigate the effect of subclinical hypothyroidism (SCH) and gestational hypertension on maternal and neonatal outcomes. 【Methods】 A total of 90 patients with gestational SCH treated in the hospital from May 2014 to March 2016 and 40 healthy volunteers (the healthy group) who received antenatal care in the same period were selected as subjects. According to the blood pressure measured after 25~28 weeks of pregnancy, the patients with gestational SCH were divided into the combined group (48 cases of SCH with gestational hypertension) and the control group (42 cases of SCH without hypertension). The thyroid function [thyroid stimulating hormone (TSH), free thyroxine (FT4), thyroid peroxidase antibody (TPO-Ab)], blood lipid levels [triglyceride (TG), serum total cholesterol (TC), low-density lipoprotein (LDLC)], maternal and neonatal outcomes, and the incidence of pregnancy complications were compared among the three groups. 【Results】 The levels of TSH and TPO-Ab in the combined group were significantly higher than those in both the control group and the healthy group, while FT4 was lower. The levels of TG, TC and LDLC were the highest in the combined group and followed by the control group, and those in the healthy group were the lowest. There were significant differences between any two groups (P＜0.05). The total incidence of adverse maternal and neonatal outcomes, and pregnancy complications in the combined group were the highest (47.92%, 31.25%), followed by the control group (16.67%, 11.90%), and the incidence was lowest in the healthy group (2.50%, 0.00%). There were significant differences between any two groups (P＜0.05). 【Conclusion】 SCH is closely related to pregnancy-induced hypertension, which may be related to abnormal lipid metabolism. The total incidence of adverse maternal and neonatal outcomes in patients with SCH complicated with pregnancy-induced hypertension is significantly higher than that in pregnant women with single disease or healthy pregnant women. 2017 Vol. 34 (7): 1313-1315 [Abstract] ( 41 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1316 Application of Precise Nursing Intervention in Improving Postoperative Inflammatory Factors and Immune Status in Patients with Renal Tumor TANG Yu-ping, LIU Yao DOI: 10.3969/j.issn.1671-7171.2017.07.022 【Objective】To study the effect of precise nursing intervention in improving postoperative inflammatory factors and immune status in patients with renal neoplasms. 【Methods】 A total of 90 cases of patients with renal tumor resection from October 2015 to December 2016 in Xiangya hospital were selected as study subjects. All of the patients were divided into two groups according to the random number methodwith 37 cases in each group. The control group was treated with routine care, and the observation group was treated with precise nursing interventions on the basis of routine nursing. The levels of interleukin-1 (IL-1), serum tumor necrosis factor (TNF-α), immunological status and renal function were analyzed before and after treatment. 【Results】There was no significant difference in IL-1 and TNF-α levels between the two groups before treatment (P＞0.05). After treatment, the levels of IL-1 and TNF-α in the two groups were lower than those before treatment, and the descending trend of the observation group was more obvious than that of control group (P＜0.05). The levels of CD4+, CD3+, CD8+, IgA, IgG and IgM in the observation group were significantly higher than those in the control group (P＜0.05). 【Conclusion】 The precise nursing intervention can effectively reduce the inflammatory reaction in the patients after renal surgery, improve the function of the immune system and renal function, and promote the prognosis of patients with rehabilitation and quality of life. It is worthwhile to put precise nursing intervention into clinical application. 2017 Vol. 34 (7): 1316-1319,1321 [Abstract] ( 60 ) HTML (1 KB)  PDF  (0 KB)  ( 14 ) 1319 Effect of Dexmedetomidine Combined with Sevoflurane Anesthesia on Circulatory Indicators and Recovery Quality in Pediatric Hernia Surgery WANG Hui, CHEN Yong-jun, XU Shen DOI: 10.3969/j.issn.1671-7171.2017.07.023 【Objective】To investigate the effect of dexmedetomidine combined with sevoflurane on the circulatory indicators and recovery quality in pediatric hernia surgery. 【Methods】 Seventy-six children with hernia surgery were randomized into two groups, with 38 cases in each. The Dexmedetomidine group (A group) was treated with intravenous injection of dexmedetomidine 0.2 μg/(kg·h) by micro pump after tracheal intubation, while the control group (B group ) was treated with intravenous infusion of normal saline in the same dose. The operation time, recovery time, extubation time and agitation scores at 0 min and 30 min after entering the recovery room were compared between the two groups. Heart rate (HR) and mean arterial pressure (MAP) were recorded before operation (T 0), after anesthesia induction (T 1), 5min (T 2) and 10min (T 3) after the beginning of operation, and the end of operation (T 4). 【Results】 There were no significant differences between the two groups in terms of recovery time and extubation time (P＞0.05). At 0 min and 30min after entering the recovery room, the agitation scores of patients in A group were lower than those in B group (P＜0.05). Compared to T 0 , HR of A group was increased at T 1 and decreased at T 2 . From T 1 ~T 4 , HR of B group increased, and MAP increased at T 1 ~T 2 (P＜0.05). From T 1 ~T 4 , HR of A group was lower than of B group, and at T 2 ~T 3 , MAP was lower than that of B group (P＜0.05). The incidence rate of emergence agitation (EA) during the recovery period of general anaesthesia in A group was 7.89% which was lower than that in B group (39.47%) (P＜0.05). 【Conclusion】 Using dexmedetomidine combined with sevoflurane in pediatric hernia surgery not only can maintain hemodynamic stability during operation, but also can improve the recovery quality, and reduce the incidence rate of EA. 2017 Vol. 34 (7): 1319-1321 [Abstract] ( 68 ) HTML (1 KB)  PDF  (0 KB)  ( 14 ) 1322 Correlation between Serum DBiL Levels and Blood Lipids in Patients with Type 2 Diabetes Mellitus LIU Qiong, WANG Bin, WANG Tian-hui,et al DOI: 10.3969/j.issn.1671-7171.2017.07.024 【Objective】To analyze the correlation between normal (0~6.89 μmol/L) serum direct bilirubin (DBiL) levels and blood lipids in patients with type 2 diabetes mellitus (T2DM) and to predict the related risk factors of dyslipidemia. 【Methods】The clinical data of 380 cases with T2DM were collected, and the levels of DBil, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), triglyceride (TG) and total cholesterol (TC) were recorded. According to the DBil levels, the subjects were divided into group A (＜ 2.2 μmol/L,n=92), group B (2.2~2.9 μmol/L,n=88), group C (2.9~3.9 μmol/L,n=102), and group D (3.9~6.89 μmol/L,n=98). The correlation between TBil and serum lipid was analyzed. 【Results】The proportions of male patients, smoking and drinking, high TC hyperlipidemia, high TG hyperlipidemia, insulin treatment, and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), postprandial 2h blood glucose (2hPG), glycosylated hemoglobin (HbA1c), total bilirubin (TBil), DBil, TC, TG and LDL-C showed significant differences among the four groups (P＜0.05). The incidence of dyslipidemia and levels of TC, TG and LDL-C in group D were lower than those in group A. The DBil level was negatively correlated with the levels of TC, TG and LDL-C in patients with T2DM (P＜0.05). The risk of dyslipidemia was the lowest in group D among the four groups. 【Conclusion】There is a correlation between normal DBil and blood lipids in patients with T2DM, and high DBil can decrease the risk of dyslipidemia. 2017 Vol. 34 (7): 1322-1324 [Abstract] ( 50 ) HTML (1 KB)  PDF  (0 KB)  ( 12 )

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