miR-202在膀胱癌细胞中的表达水平及其对膀胱癌细胞增殖、凋亡和侵袭的影响

doi:10.3969/j.issn.1671-7171.2022.11.023

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (1251 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要【目的】探讨miR-202在膀胱癌细胞中的表达水平及其对膀胱癌细胞增殖、凋亡和侵袭的影响。【方法】实时荧光定量聚合酶链反应(qRT-PCR)检测膀胱细胞中miR-202的相对表达水平;转染技术将miR-202和miR-NC质粒转染至UM-UC-3细胞中,并设置为miR-202组和NC组。细胞增殖实验、细胞侵袭实验和凋亡实验分别检测两组细胞的增殖、迁移、侵袭和凋亡情况,比较两组裸鼠移植瘤体积和重量,Western blot实验检测两组细胞中PIK3CA蛋白的表达情况。【结果】膀胱癌细胞中miR-202的相对表达水平分别低于正常膀胱上皮细胞,差异有统计学意义(P<0.05)。miR-202组与NC组相比细胞的OD值、迁移和发生侵袭的细胞数降低,凋亡率增加,差异有统计学意义(均P<0.05)。miR-202组移植瘤的体积和重量均低于NC组,差异有统计学意义。miR-202组PIK3CA蛋白的表达低于NC组,差异有统计学意义。【结论】miR-202可能通过抑制PIK3CA蛋白的表达从而抑制膀胱癌细胞的增殖、迁移和侵袭并诱导细胞凋亡。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章

关键词 ：膀胱肿瘤/病理学, 微RNAs, 细胞增殖, 细胞凋亡, 肿瘤浸润

Abstract：【Objective】To investigate the expression of miR-202 in bladder cancer cells and its effect on proliferation, apoptosis and invasion of bladder cancer cells. 【Methods】The relative expression of miR-202 in bladder cells was detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR); The miR-202 and miR-NC plasmids were transfected into UM-UC-3 cells by transfection technology and set as miR-202 group and NC group. Cell proliferation test, cell invasion test and apoptosis test were used to detect the proliferation, migration, invasion and apoptosis of cells in the two groups respectively. The volume and weight of transplanted tumors in the two groups were compared. Western blot test was used to detect the expression of PIK3CA protein in the cells of the two groups. 【Results】The relative expression level of miR-202 in bladder cancer cells was lower than that in normal bladder epithelial cells (P<0.05). Compared with NC group, the OD value, migration and number of invasive cells in the miR-202 group decreased, and the apoptosis rate increased, with significant difference (all P<0.05). The volume and weight of transplanted tumor in the miR-202 group were lower than those in the NC group, and the difference was statistically significant. The expression of PIK3CA protein in the miR-202 group was lower than that in the NC group, and the difference was statistically significant. 【Conclusion】miR-202 may inhibit the proliferation, migration, invasion and induce apoptosis of bladder cancer cells by inhibiting the expression of PIK3CA protein.

Key words： Urinary Bladder Neoplasms/PA MicroRNAs Cell Proliferation Apoptosis Neoplasm Invasiveness

收稿日期: 2021-09-28

中图分类号:

R694

通讯作者: *E-mail:hero119525@126.com

引用本文:

钱雄贤, 禇健, 赵勇, 李立阳, 叶茂飞. miR-202在膀胱癌细胞中的表达水平及其对膀胱癌细胞增殖、凋亡和侵袭的影响[J]. 医学临床研究, 2022, 39(11): 1680-1682.
QIAN Xiong-xian, CHU Jian, ZHAO Yong, et al. Expression of miR-202 in Bladder Cancer Cells and its Effect on Proliferation, Apoptosis and Invasion of Bladder Cancer Cells. JOURNAL OF CLINICAL RESEARCH, 2022, 39(11): 1680-1682.

链接本文:

http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2022.11.023 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2022/V39/I11/1680

[1] HUANG M,ZHONG Z,LV M,et al. Comprehensive analysis of differentially expressed profiles of lncRNAs and circRNAs with associated coexpression and ceRNA networks in bladder carcinoma[J].Oncotarget,2016,7(30):47186-47200.
[2] MCCONKEY D J,CHOI W,OCHOA A,et al. Intrinsic subtypes and bladder cancer metastasis[J].Asian J Urol,2016,3(4):260-267.
[3] BARTEL D P. MicroRNAs:target recognition and regulatory functions[J].Cell,2009,136(2):215-233.
[4] MENG X,CHEN X,LU P,et al. miR-202 promotes cell apoptosis in esophageal squamous cell carcinoma by targeting HSF2[J].Oncol Res,2017,25(2):215-223.
[5] LIVAK K J,SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2[-Delta Delta C(T)] method[J].Methods,2001,25(4):402-408.
[6] ROUPRÊT M. WORDS OF WISDOM. Finasteride reduces the risk of bladder cancer in a large prospective screening study[J].Eur Urol,2016,69(3):537.
[7] MATSUYAMA H,SUZUKI H I. Systems and synthetic microRNA biology:from biogenesis to disease pathogenesis[J].Int J Mol Sci,2019,21(1):132.
[8] AVGERIS M,PANOUTSOPOULOU K,PAPADIMITRIOU M A,et al. Circulating exosomal miRNAs:clinical significance in human cancers[J].Expert Rev Mol Diagn,2019,19(11):979-995.
[9] SUN Z,ZHANG T,HONG H,et al. miR-202 suppresses proliferation and induces apoptosis of osteosarcoma cells by downregulating Gli2[J].Mol Cell Biochem,2014,397(1-2):277-283.
[10] WANG Q,HUANG Z,GUO W,et al. microRNA-202-3p inhibits cell proliferation by targeting ADP-ribosylation factor-like 5A in human colorectal carcinoma[J].Clin Cancer Res,2014,20(5):1146-1157.
[11] LIU B,ZHU Z. Decrease of miR-202-3p expression,a novel tumor suppressor,in gastric cancer[J].PLoS One,2013,8(7):e69756.
[12] CHAI R,FU L,ZHENG Z,et al. Resveratrol inhibits proliferation and migration through SIRT1 mediated post-translational modification of PI3K/AKT signaling in hepatocellular carcinoma cells[J].Mol Med Report,2017,16(6):8037-8044.
[13] DENG L,ZHU X,SUN Y,et al.Prevalence and prognostic role of PIK3CA/AKT1 mutations in Chinese breast cancer patients[J].Cancer Res Treat,2019,51(1):128-140.
[14] LI X,YANG T,LI C S,et al. Surface Enhanced Raman Spectroscopy (SERS)for the Multiplex Detection of Braf,Kras,and Pik3ca mutations in plasma of colorectal cancer patients[J].Theranostics,2018,8(6):1678-1689.