医学临床研究
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医学临床研究  2022, Vol. 39 Issue (6): 889-892    DOI: 10.3969/j.issn.1671-7171.2022.06.024
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溃疡性结肠炎患者外周血miR-21表达及其与Th17/Treg平衡的相关性
张怡, 酒梦娜*
安康市中心医院,陕西 安康 725000
Expression of miR-21 in Peripheral Blood of Patients with Ulcerative Colitis and its Correlation with Th17/Treg Balance
ZHANG Yi, JIU Meng-na
Ankang Central Hospital,Ankang Shaanxi 725000
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摘要 【目的】探讨溃疡性结肠炎患者血浆微小RNA-21(miR-21)与外周血单个核细胞辅助性T细胞亚群17(Th17)细胞、调节性T细胞(Treg)水平的相关性。【方法】选取2017年8月至2019年12月本院收治的102例溃疡性结肠炎患者(观察组),选择同期于本院体检的114例健康志愿者(对照组)。采用实时荧光定量PCR(qRT-PCR)法检测血浆miR-21水平,流式细胞术检测Th17细胞、Treg细胞水平,采用酶联免疫吸附(ELISA)法检测血浆白介素-17(IL-17)、转化生长因子-β(TGF-β)、白介素-10(IL-10)、白介素-21(IL-21)水平;分析溃疡性结肠炎患者血浆炎性因子水平与miR-21、Th17/Treg水平的相关性,分析影响溃疡性结肠炎发生的因素。【结果】观察组miR-21、Th17细胞、Th17/Treg水平高于对照组,Treg细胞水平低于对照组,差异有统计学意义(P<0.05)。观察组IL-17、IL-21水平高于对照组,TGF-β、IL-10水平低于对照组,差异有统计学意义(P<0.05)。Pearson法分析结果显示,溃疡性结肠炎患者miR-21与Th17/Treg水平呈正相关(r=0.598,P<0.05),miR-21、Th17/Treg水平与IL-17、IL-21水平呈正相关,与TGF-β、IL-10水平呈负相关(P<0.05)。Logistic多因素回归分析显示,miR-21、Th17/Treg是影响溃疡性结肠炎发生的独立危险因素(P<0.05),TGF-β是影响溃疡性结肠炎发生的保护因素(P<0.05)。【结论】溃疡性结肠炎患者Th17/Treg处于严重失衡状态,且Th17/Treg失衡状态可能与miR-21水平密切相关。
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张怡
酒梦娜
关键词 结肠炎,溃疡性/病理学微RNAsT淋巴细胞,调节性T淋巴细胞亚群    
Abstract:【Objective】To investigate the correlation between plasma microRNA-21 (miR-21) ,peripheral blood mononuclear cell helper T cell subsets 17 (Th17) cells and regulatory T cells (Treg) in patients with ulcerative colitis. 【Methods】 A total of 102 patients with ulcerative colitis (observation group) admitted to our hospital from August 2017 to December 2019 were selected,and 114 healthy volunteers (control group) who underwent physical examination in our hospital at the same time were selected. The levels of miR-21 in plasma were detected by real-time fluorescent quantitative PCR (qRT-PCR),Th17 cells and Treg cells were detected by flow cytometry,and the levels of interleukin-17 (IL-17) and transforming growth factor-β(TGF-β) in plasma were detected by enzyme-linked immunosorbent assay (ELISA),levels of interleukin-10 (IL-10) and interleukin-21 (IL-21); the correlation between plasma inflammatory factor levels and miR-21,Th17/Treg levels in patients with ulcerative colitis,and the factors affecting the occurrence of ulcerative coliti were analyzed. 【Results】 The levels of miR-21,Th17 cells and Th17/Treg in the observation group were higher than those in the control group,and the levels of Treg cells were lower than those in the control group (P<0.05). The levels of IL-17 and IL-21 in the observation group were higher than those in the control group,The level of TGF-β and IL-10 was significantly lower than that of the control group (P<0.05). Pearson analysis showed that the levels of miR-21 and Th17/Treg in patients with ulcerative colitis were positively correlated (r=0.598,P<0.05),and the levels of miR-21 and Th17/Treg were positively correlated with the levels of IL-17 and IL-21,and was negatively correlated with the level of TGF-β and IL-10 (P<0.05). Logistic regression analysis showed that miR-21 and Th17/Treg were independent risk factors for ulcerative colitis (P<0.05). TGF-β was the protective factor of ulcerative colitis (P<0.05). 【Conclusion】 Patients with ulcerative colitis have a serious imbalance of Th17/Treg,and the imbalance of Th17/Treg may be closely related to the level of miR-21.
Key wordsColitis, Ulcerative    MicroRNAs    T-Lymphocytes, Regulatory    T-Lymphocyte Subsets
收稿日期: 2021-12-17     
中图分类号:  R574.621  
通讯作者: *E-mail:1049931056@qq.com   
引用本文:   
张怡, 酒梦娜. 溃疡性结肠炎患者外周血miR-21表达及其与Th17/Treg平衡的相关性[J]. 医学临床研究, 2022, 39(6): 889-892.
ZHANG Yi, JIU Meng-na. Expression of miR-21 in Peripheral Blood of Patients with Ulcerative Colitis and its Correlation with Th17/Treg Balance. JOURNAL OF CLINICAL RESEARCH, 2022, 39(6): 889-892.
链接本文:  
http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2022.06.024     或     http://journal07.magtech.org.cn/yxlcyj/CN/Y2022/V39/I6/889
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