Abstract：【Objective】 To investigate the effects of microRNA (miR)-21-5p on the proliferation, migration, invasion, signal transduction and activator of transcription 3 (STAT3) signal pathway of multiple myeloma (MM). 【Methods】 Human MM RPMI-8226 cells were cultured in vitro and divided into control group (without transfection), siRNA NC group (transfected with nonsense siRNA) and miR-21-5p siRNA group (transfected with miR-21-5p siRNA). The expressions of miR-21-5p and STAT3 in RPMI-8226 cells were detected by real-time fluorescence quantitative PCR (qRT-PCR); The proliferation of RPMI-8226 cells was detected by CCK-8 method; The migration and invasion of RPMI-8226 cells were detected by Transwell method; Western blot (WB) was used to detect the expression of p-STAT3, STAT3, Ki-67 and matrix metalloproteinase-9 (MMP-9) in RPMI-8226 cells.【Results】 Compared with the control group and the siRNA NC group, the expression levels of miR-21-5p, STAT3 mRNA, cell OD value, number of migrating cells, number of invasive cells, p-STAT3/STAT3, Ki-67 and MMP-9 protein in RPMI-8226 cells in the miR-21-5p siRNA group were significantly lower (P<0.05). 【Conclusion】 Down-regulating the expression of miR-21-5p can inhibit the proliferation, migration and invasion of MM RPMI-8226 cells, and block the activation of STAT3 signaling pathway.
白涛, 鲁良, 郭晓宁, 宫惠琳. miR-21-5p对多发性骨髓瘤细胞增殖、迁移、侵袭及STAT3信号通路的影响[J]. 医学临床研究, 2022, 39(5): 671-674.
BAI Tao, LU Liang, GUO Xiao-ning, et al. Effects of miR-21-5p on Proliferation, Migration, Invasion and STAT3 Signaling Pathway of Multiple Myeloma Cells. JOURNAL OF CLINICAL RESEARCH, 2022, 39(5): 671-674.
[1] BARWICK B G, GUPTA V A, VERTINO P M, et al. Cell of origin and genetic alterations in the pathogenesis of multiple myeloma[J].Front Immunol,2019, 10(1):1121-1201. [2] YANAMANDRA U, KUMAR S K. Minimal residual disease analysis in myeloma-when, why and where[J].Leuk Lymphoma,2018, 59(8):1772-1784. [3] SEKAR D, KRISHNAN R. Significance of microRNA 21 in gastric cancer[J].Clin Res Hepatol Gastroenterol,2016, 40(5):538-545. [4] 宋慧慧, 李原, 马钰, 等. 靶向抑制miR-21对多发性骨髓瘤细胞凋亡、增殖的影响及其作用机制的初步研究[J].东南大学学报(医学版),2018, 37(5):77-81. [5] WANG Y, WU L, CAI H, et al. YL064 directly inhibits STAT3 activity to induce apoptosis of multiple myeloma cells[J].Cell Death Discov,2019, 4(1):44-75. [6] 张熙辉, 龚健, 黄胜, 等. Jagged1调控STAT3信号通路影响多发性骨髓瘤细胞的生长和凋亡[J].中国病理生理杂志, 2018, 34(7):1232-1238. [7] YANG Y, WANG S, LI J, et al. CUL4A as a marker and potential therapeutic target in multiple myeloma[J].Tumour Biol,2017, 39(7):1-7. [8] QIAN C, XIN L, JINGRU L, et al. Multiple myeloma-derived exosomes regulate the functions of mesenchymal stem cells partially via modulating miR-21 and miR-146a[J].Stem Cells Int,2017, 2017(1):1-9. [9] 袁中健, 王龙, 卢秀娟, 等. miRNA和Th17相关细胞因子在多发性骨髓瘤患者发病中的作用[J].国际免疫学杂志, 2019, 42(6):583-587. [10] WANG J H, ZHOU W W, LIU B X, et al. Expression and significance of miR-21 in multiple myeloma patients[J].Genet Mol Res,2016, 15(1):1-8. [11] PING L, XUE W, LU D, et al. Gambogenic acid exerts antitumor activity in hypoxic multiple myeloma cells by regulation of miR-21[J].J Cancer,2017, 8(16):3278-3286. [12] 黄朝友, 李响, 赖飞, 等. 肾母细胞瘤组织中Par-4基因表达量检测及其与肿瘤增殖、侵袭的关系研究[J].陕西医学杂志, 2018, 47(10):8-10. [13] 吴迪, 刘芳, 余艳丽, 等. 大蒜素抑制人类多发性骨髓瘤细胞增殖和侵袭能力的实验研究[J].国际肿瘤学杂志, 2018, 45(6):325-330. [14] 邹杨, 刘园, 黄薇, 等. STAT3抗肿瘤抑制剂的研究进展[J].药学学报, 2018, 53(10):20-30.
2022, Vol. 39 
