干扰PARP1基因表达对胃癌MGC-803细胞增殖、侵袭及迁移的影响

doi:10.3969/j.issn.1671-7171.2022.05.018

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摘要【目的】探讨多聚腺苷二磷酸核糖聚合酶1(PARP1)基因对胃癌细胞(MGC-803)增殖、迁移、侵袭的影响。【方法】体外培养人胃癌细胞系MGC-803细胞和人正常胃黏膜上皮细胞GES-1,比较两种细胞PARP1 mRNA表达差异;将MGC-803细胞随机分为对照组、干扰PARP1表达组(si-PARP1组)及阴性对照组(si-NC组);qRT-PCR法检测各组MGC-803细胞PARP1 mRNA表达;MTT法、流式细胞术、划痕实验、Transwell小室法分别检测细胞增殖、凋亡、迁移与侵袭,Western blot检测PARP1、基质金属蛋白酶-2(MMP-2)、MMP-9、RhoA、Rock1蛋白表达。【结果】与GES-1比较,MGC-803细胞PARP1 mRNA表达显著升高(P<0.05);与对照组和si-NC组相比,si-PARP1组MGC-803细胞凋亡率和增殖抑制率显著升高(P<0.05),细胞划痕迁移率、侵袭率、PARP1 mRNA和PARP1蛋白、MMP-2、MMP-9、RhoA、Rock1蛋白表达水平显著降低(P<0.05)。【结论】干扰PARP1表达可抑制人胃癌细胞细胞增殖、迁移及侵袭,其可能与抑制RhoA/Rock信号通路有关。

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	曹秋祥
	南虹
	潘晓涛

关键词 ：胃肿瘤/病理学, 基因表达, 肿瘤转移, 细胞增殖

Abstract：【Objective】 To investigate the effect of poly adenosine diphosphate ribose polymerase 1 (PARP1) gene on the proliferation, migration and invasion of gastric cancer cell line (MGC-803).【Methods】 Human gastric cancer cell line MGC-803 and human normal gastric mucosal epithelial cell GES-1 were cultured in vitro, and the expression of PARP1 mRNA was compared between the two cells; MGC-803 cells were randomly divided into control group, interference PARP1 expression group (si-PARP1 group) and negative control group (si-NC group); The expression of PARP1 mRNA in MGC-803 cells was detected by qRT-PCR; MTT assay, flow cytometry, scratch test and Transwell chamber method were used to detect cell proliferation, apoptosis, migration and invasion respectively. Western blot was used to detect the expression of PARP1, matrix metalloproteinase-2 (MMP-2), MMP-9, RhoA and Rock1 proteins. 【Results】 Compared with GES-1, the expression of PARP1 mRNA in MGC-803 cells increased significantly (P<0.05); Compared with the control group and the si-NC group, the apoptosis rate and proliferation inhibition rate of MGC-803 cells in the si-PARP1 group increased significantly (P<0.05), and the migration rate and invasion rate of cell scratch, the expression levels of PARP1 mRNA and PARP1 protein, MMP-2, MMP-9, RhoA and Rock1 protein decreased significantly (P<0.05). 【Conclusion】 Interfering with the expression of PARP1 can inhibit the proliferation, migration and invasion of human gastric cancer cells, which may be related to the inhibition of RhoA/Rock signaling pathway.

Key words： Stomach Neoplasms/PA Gene Expression Neoplasm Metastasis Cell Proliferation

收稿日期: 2021-12-10

中图分类号:

R735.2

通讯作者: *E-mail:708805858@qq.com

引用本文:

曹秋祥, 南虹, 潘晓涛. 干扰PARP1基因表达对胃癌MGC-803细胞增殖、侵袭及迁移的影响[J]. 医学临床研究, 2022, 39(5): 705-708.
CAO Qiu-xiang, NAN Hong, PAN Xiao-tao. Effects of Interfering with PARP1 Gene Expression on Proliferation, Invasion and Migration of Gastric Cancer MGC-803 Cells. JOURNAL OF CLINICAL RESEARCH, 2022, 39(5): 705-708.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2022.05.018 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2022/V39/I5/705