Abstract：【Objective】 To investigate the effect of poly adenosine diphosphate ribose polymerase 1 (PARP1) gene on the proliferation, migration and invasion of gastric cancer cell line (MGC-803).【Methods】 Human gastric cancer cell line MGC-803 and human normal gastric mucosal epithelial cell GES-1 were cultured in vitro, and the expression of PARP1 mRNA was compared between the two cells; MGC-803 cells were randomly divided into control group, interference PARP1 expression group (si-PARP1 group) and negative control group (si-NC group); The expression of PARP1 mRNA in MGC-803 cells was detected by qRT-PCR; MTT assay, flow cytometry, scratch test and Transwell chamber method were used to detect cell proliferation, apoptosis, migration and invasion respectively. Western blot was used to detect the expression of PARP1, matrix metalloproteinase-2 (MMP-2), MMP-9, RhoA and Rock1 proteins. 【Results】 Compared with GES-1, the expression of PARP1 mRNA in MGC-803 cells increased significantly (P<0.05); Compared with the control group and the si-NC group, the apoptosis rate and proliferation inhibition rate of MGC-803 cells in the si-PARP1 group increased significantly (P<0.05), and the migration rate and invasion rate of cell scratch, the expression levels of PARP1 mRNA and PARP1 protein, MMP-2, MMP-9, RhoA and Rock1 protein decreased significantly (P<0.05). 【Conclusion】 Interfering with the expression of PARP1 can inhibit the proliferation, migration and invasion of human gastric cancer cells, which may be related to the inhibition of RhoA/Rock signaling pathway.
曹秋祥, 南虹, 潘晓涛. 干扰PARP1基因表达对胃癌MGC-803细胞增殖、侵袭及迁移的影响[J]. 医学临床研究, 2022, 39(5): 705-708.
CAO Qiu-xiang, NAN Hong, PAN Xiao-tao. Effects of Interfering with PARP1 Gene Expression on Proliferation, Invasion and Migration of Gastric Cancer MGC-803 Cells. JOURNAL OF CLINICAL RESEARCH, 2022, 39(5): 705-708.
[1] 翟宏芳, 刘丽丽, 刘爱娟, 等.miR-582-5p靶向PDCD10对胃癌细胞迁移、侵袭及凋亡的影响[J].山东医药, 2020, 60(28):37-40.
[2] BARREIRO E, GEA J. PARP-1 and PARP-2 activity in cancer-induced cachexia:potential therapeutic implications[J].Biol Chem,2018, 399(2):179-186.
[3] LIU Y, ZHANG Y, ZHAO Y, et al. High PARP-1 expression is associated with tumor invasion and poor prognosis in gastric cancer[J].Oncol Lett,2016, 12(5):3825-3835.
[4] 高阳, 杨帆.MiR-26b靶向hENT1通过RhoA/ROCK-1通路调控肺癌细胞的侵袭和迁移[J].中南大学学报(医学版), 2017, 42(7):755-761.
[5] CHEN Z, LIU S, XIA Y, et al. MiR-31 regulates rho-associated kinase-myosin light chain (ROCK-MLC) pathway and inhibits gastric cancer invasion:roles of RhoA[J].Med Sci Monit, 2016, 22(1):4679-4691.
[6] 蒋茂芬, 李君强, 刘春姣, 等. Klotho过表达对胃癌SGC-7901细胞增殖、迁移及PI3K/AKT通路的影响[J].重庆医学, 2020, 49(13):2065-2070.[7] SON D I, HONG S, SHIN K S, et al. PARP-1 regulates mouse embryonic neural stem cell proliferation by regulating PDGFRα expression[J].Biochem Biophys Res Commun,2020,526(4):986-992.
[8] 许琦华. 胃癌患者血清中DKK-1、HE4、PARP-1水平与胃癌临床分期、淋巴结转移度及临床预后的关系[J].国际检验医学杂志, 2017, 38(23):3219-3221.
[9] 费晶, 巩平. 干扰PARP-1基因对人胃癌HGC-27细胞增殖,凋亡及放射敏感性的影响[J].郑州大学学报(医学版), 2020, 55(4):537-541.
[10] KIM J G, KIM M J, CHOI W J, et al. Wnt3A induces GSK-3β phosphorylation and β-Catenin accumulation through RhoA/ROCK[J].J Cell Physiol,2017, 232(5):1104-1113.
[11] MU G, DING Q, LI H, et al. Gastrin stimulates pancreatic cancer cell directional migration by activating the Gα12/13-RhoA-ROCK signaling pathway[J].Exp Mol Med,2018, 50(5):1-14.
[12] 廖山婴, 刘超, 王蓓蓓, 等.RhoA/ROCK信号通路在胃癌中的表达及其与胃癌生物学行为的关系[J].胃肠病学和肝病学杂志, 2017, 26(3):251-255.
[13] 蒋亦雯, 洪丹, 楼哲丰, 等.羽扇豆醇通过抑制RhoA-ROCK1信号通路抑制结肠癌细胞增殖[J].中国细胞生物学学报, 2020, 42(2):186-194.
2022, Vol. 39 
