Abstract：【Objective】To explore the expression of miR-200b-5p and Adenosine triphosphate family protein 2 (ATAD2) in cervical cancer tissues and their relationship with prognosis. 【Methods】A total of 124 patients with cervical cancer who underwent surgical treatment in our hospital from January 2019 to March 2020 were selected in the study. All cervical cancer tissue specimens and para-cancerous tissues (≥5 cm from the cancerous tissue) were collected. The expression levels of miR-200b-5p mRNA and ATAD2 mRNA in cancer tissues and adjacent tissues were compared. After 18 months follow-up, the prognosis of cervical cancer patients was counted and divided into the death group and the survival group according to the prognosis. Compare the clinical data of patients in the death group and survival group. Logistic multivariate regression was used to analyze the risk factors affecting the prognosis of cervical cancer patients. A receiver operating characteristic curve (ROC) was made, and the area under the curve (AUC) was used to analyze the predictive power of the expression of miR-200b-5p and ATAD2 in cervical cancer tissues on the prognosis of cervical cancer patients. 【Results】The miR-200b-5p mRNA in cervical cancer tissue was lower than that in adjacent tissues (P<0.05), while ATAD2 mRNA was higher than that in adjacent tissues (P<0.05). After 18 months of follow-up, the death rate of cervical cancer patients was 17.74%. In the death group, the proportion of stage Ⅲ/Ⅳ, the proportion of lymph node metastasis, the proportion of poorly differentiated degree, the proportion of muscle invasion depth>1/2, the proportion of ATAD2 mRNA in cervical cancer tissue was higher than those in the survival group (P<0.05), while cervical cancer tissue miR-200b-5p level was lower than in the survival group (P<0.05). Logistic regression analysis showed that the clinical stage Ⅲ/Ⅳ, poorly differentiated, low expression level of miR-200b-5p mRNA and high level ATAD2 mRNA in cervical cancer tissues were all risk factors affecting the prognosis of cervical cancer patients (OR=2.835, 2.793 , 3.080, 3.364, respectively, P<0.05). ROC analysis showed that the AUCs of miR-200b-5p mRNA and ATAD2 mRNA in cervical cancer tissues to predict the prognosis of cervical cancer death were 0.793 and 0.860, respectively. 【Conclusion】The level of miR-200b-5p mRNA is low in cervical cancer tissues, while ATAD2 mRNA level is high. Both miR-200b-5p mRNA and ATAD2 mRNA in cervical cancer tissue have a high predictive power for the prognosis of cervical cancer patients. Thus they can be used as important reference indicators for evaluating the cervical cancer prognosis.
刘平, 陆美荣, 胡娜. miR-200b-5p、ATAD2在宫颈癌组织中的表达水平及其与患者预后的关系[J]. 医学临床研究, 2022, 39(8): 1182-1185.
LIU Ping, LU Mei-rong, HU Na. Expression of miR-200b-5p and ATAD2 in Cervical Cancer Tissues and Their Relationship with Prognosis. JOURNAL OF CLINICAL RESEARCH, 2022, 39(8): 1182-1185.
[1] 叶俊良,陈晓宇,赵卫东,等. EphA2和Twist蛋白在宫颈病变中的表达及与宫颈癌患者预后的关系[J].实用医学杂志,2019,35(15):2424-2428. [2] OLUSOLA P,BANERJEE H N,PHILLEY J V,et al. Human papilloma virus-associated cervical cancer and health disparities[J].Cells,2019,8(6):28-32. [3] WANG A Q,LV M,XU Y H,et al. MiR-200b-5p inhibits proliferation of ovarian cancer cells by targeting ATAD2 and regulating PI3K/AKT signaling pathway[J].Eur Rev Med Pharmacol Sci,2020,24(19):9860-9868. [4] LI W,JIA M X,DENG J,et al. Down-regulation of microRNA-200b is a potential prognostic marker of lung cancer in southern-central Chinese population[J].Saudi J Biol Sci,2019,26(1):173-177. [5] LIU Q,LIU H,LI L,et al. ATAD2 predicts poor outcomes in patients with ovarian cancer and is a marker of proliferation[J].Int J Oncol,2020,56(1):219-231. [6] 朱晓志,王冬梅,王丽蓉. miR-200b-5p靶向ATAD2调控P13K/AKT信号通路参与宫颈癌血管生成的作用机制[J].中国性科学,2019,28(7):42-46. [7] 中华医学会病理学分会女性生殖系统疾病学组,中国优生科学协会阴道镜与宫颈病理学会病理学组. 宫颈癌及癌前病变病理诊断规范[J].中华病理学杂志,2019,48(4):265-269. [8] LEE S I,ATRI M. 2018 FIGO Staging system for uterine cervical cancer:enter cross-sectional imaging[J].Radiology,2019,292(1):15-24. [9] CURTY G,DE CARVALHO P S,SOARES M A. The role of the cervicovaginal microbiome on the genesis and as a biomarker of premalignant cervical intraepithelial neoplasia and invasive cervical cancer[J].Int J Mol Sci,2019,21(1):36-42. [10] 赵方,胡昌华,张远远. 联合检测血清VEGF、Ki67、PD-L1表达水平评估宫颈癌患者预后的价值[J].中国妇幼保健,2019,34(14):3206-3209. [11] 唐慧琳,郭秀娟,应春妹. 鳞状细胞癌相关抗原、CA153、CA199联合检测对宫颈癌诊断及预后判断的临床价值[J].中国妇幼保健,2019,34(17):3935-3937. [12] PARK J Y,CHONG G O,PARK J Y,et al. Tumor budding in cervical cancer as a prognostic factor and its possible role as an additional intermediate-risk factor[J].Gynecol Oncol,2020,159(1):157-163. [13] OTERO-GARCIA M M,MESA-ALVAREZ A,NIKOLIC O,et al. Role of MRI in staging and follow-up of endometrial and cervical cancer:pitfalls and mimickers[J].Insights Imaging,2019,10(1):19-23. [14] CHANG C,CHEN J,HUANG C H,et al. Time-dependent squamous cell carcinoma antigen in prediction of relapse and death of patients with cervical cancer[J].J Low Genit Tract Dis,2020,24(1):38-42. [15] GAY J C,ECKENROTH B E,EVANS C M,et al. Disulfide bridge formation influences ligand recognition by the ATAD2 bromodomain[J].Proteins,2019,87(2):157-167. [16] 郑乐. ATAD2基因对宫颈癌增殖和侵袭迁移能力影响的相关研究[D]. 沈阳:中国医科大学,2016.
2022, Vol. 39 
