Abstract：【Objective】 To investigate the expression levels of Toll-like receptor-4(TLR-4) and high mobility group protein 1 (HMGB1) in peripheral blood of patients with preeclampsia and their diagnostic value for renal injury. 【Methods】A total of 98 patients with preeclampsia diagnosed and treated in Xi'an International Medical Center Hospital from June 2020 to December 2021 (observation group) were selected, and 92 healthy pregnant women (control group) who came to the hospital for pregnancy examination at the same time were selected. The expression levels of TLR-4 and HMGB1 in the peripheral blood of the two groups of pregnant women were compared. According to the occurrence of renal injury in patients with preeclampsia, they were divided into the concurrent group and the non concurrent group. The expression level and clinical characteristics of TLR-4 and HMGB1 in peripheral blood of pregnant women in the concurrent group and the non concurrent group were compared. Logistic regression was used to analyze the influencing factors of renal injury in preeclampsia patients, and the diagnostic value of TLR-4 and HMGB1 expression in peripheral blood in preeclampsia patients with renal injury was analyzed using the ROC curve. 【Results】 The expression levels of TLR-4 and HMGB1 in peripheral blood of pregnant women in the observation group were higher than those in the control group (P<0.05); The incidence of renal injury in the observation group was 23.47%, and the expression levels of TLR-4 and HMGB1 in the peripheral blood of the pregnant women with complications were higher than those of the pregnant women without complications (P<0.05); The 24 hour urine protein content and serum creatinine level of the pregnant women in the concurrent group were significantly higher than those in the non concurrent group (P<0.05). Logistic regression analysis showed that TLR-4, HMGB1 levels in peripheral blood and 24h urine protein content were independent risk factors for renal injury in patients with preeclampsia (P<0.05); ROC curve analysis showed that the optimal cut-off points for TLR-4 and HMGB1 expression levels in peripheral blood to diagnose renal injury in patients with preeclampsia were 3.54 ng/mL and 18.40 ng/mL, respectively, with sensitivity of 78.26%, 82.61%, specificity of 74.67%, 70.67%, and area under the curve (AUC) of 0.748, 0.782. The combined specificity and AUC of the two were 93.33% and 0.872, respectively. 【Conclusion】 The expression levels of TLR-4 and HMGB1 in peripheral blood of pre eclampsia pregnant women are abnormally elevated, and both of them are independent risk factors for renal injury in pre eclampsia pregnant women. The clinical detection of TLR-4 and HMGB1 expression levels in peripheral blood of pre eclampsia pregnant women can be used as sensitive indicators for diagnosing renal injury in pre eclampsia pregnant women.
赵静, 毛小梅. 子痫前期患者外周血中TLR-4、HMGB1的表达水平及其对肾损伤的诊断价值[J]. 医学临床研究, 2022, 39(10): 1501-1504.
ZHAO Jing, MAO Xiao-mei. Expression of TLR-4 and HMGB1 in Peripheral Blood of Patients with Preeclampsia and its Diagnostic Value for Rena Injury. JOURNAL OF CLINICAL RESEARCH, 2022, 39(10): 1501-1504.
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2022, Vol. 39 
