丹酚酸B对慢性肾盂肾炎大鼠肾间质损伤的影响及机制

doi:10.3969/j.issn.1671-7171.2022.03.013

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摘要【目的】探讨丹酚酸B对慢性肾盂肾炎大鼠肾间质损伤的影响及机制。【方法】将40只SD大鼠分为对照组(生理盐水灌胃)、模型组(生理盐水灌胃)、丹酚酸B组(12.5 mg/kg丹酚酸B灌胃)、激活剂组(12.5 mg/kg丹酚酸B灌胃+ 2.5 mg/kg Smad3激活剂SRI-011381腹腔注射),每组10只。模型组、丹酚酸B组、激活剂组建立慢性肾盂肾炎大鼠模型,建模次日开始给药,每日1次,连续6周。检测各组大鼠血肌酐、24 h尿蛋白含量、肾组织中α平滑肌肌动蛋白(α-SMA)、Smad3、转化生长因子-β1(TGF-β1)的表达情况。【结果】与对照组比较,模型组24 h尿蛋白含量升高(P<0.05);与对照组比较,模型组肾组织中α-SMA表达量升高(P<0.05);与模型组比较,丹酚酸B组及激活剂组肾组织中α-SMA表达量降低,且丹酚酸B组低于激活剂组(P<0.05)。与对照组比较,模型组肾组织中p-Smad3、TGF-β1、α-SMA蛋白相对表达量升高(P<0.05);与模型组肾组织中p-Smad3、TGF-β1、α-SMA蛋白相对表达量比较,丹酚酸B组及激活剂组均降低,且丹酚酸B组低于激活剂组(P<0.05)。【结论】丹酚酸B可抑制慢性肾盂肾炎大鼠肾间质纤维化,其调控机制可能与抑制Smad3信号通路有关。

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	刘长林
	周彬
	李永亮

关键词 ：肾盂肾炎, 疾病模型,动物, 大鼠, 肾/损伤

Abstract：【Objective】 To investigate the effect of salvianolic acid B on renal interstitial injury in rats with chronic pyelonephritis and its mechanism. 【Methods】 Forty SD rats were divided into control group (normal saline gavage), model group (normal saline gavage), salvianolic acid B group (12.5 mg/kg salvianolic acid B gavage) and activator group (12.5 mg/kg salvianolic acid B gavage + 2.5 mg/kg Smad3 activator SRI-011381 intraperitoneal injection), with 10 rats in each group. The rat model of chronic pyelonephritis was established in the model group,the salvianolic acid B group and the activator group. The drug was administered once a day for 6 weeks from the next day. Expression of the contents of serum creatinine, 24-hour urinary protein and renal tissue,αSmooth muscle actin(α-SMA), Smad3, transforming growth factor-β1(TGF-β1) were measured. 【Results】Compared with the control group, the 24-hour urinary protein content in the model group increased (P<0.05); Compared with the control group, the expression of α-SMA of the renal tissue in the salvianolic acid group B and the activator group decreased(P<0.05); Compared with the control group, the expression of α-SMA in the salvianolic acid group B was lower than that in the activator group(P<0.05). Compared with the control group, the relative expression of P-smad3,TGF-β1 andα-SMA protein of renal tissue in the model group increased(P<0.05); Compared with the relative expression of p-Smad3,α-SMA protein and TGF-β1 in kidney tissue of the model group, those of the salvianolic acid group B and the activator group decreased, and those of the salvianolic acid group B was lower than those in the activator group (P<0.05). 【Conclusion】 Salvianolic acid B can inhibit renal interstitial fibrosis in rats with chronic pyelonephritis, and its regulatory mechanism may be related to the inhibition of Smad3 signal pathway.

Key words： Pyelonephritis Disease Models, Animal Rats Kidney/IN

收稿日期: 2021-09-13

中图分类号:

R692.31

引用本文:

刘长林, 周彬, 李永亮. 丹酚酸B对慢性肾盂肾炎大鼠肾间质损伤的影响及机制[J]. 医学临床研究, 2022, 39(3): 366-369.
LIU Chang-lin, ZHOU Bin, LI Yong-liang. Effects of Salvianolic Acid B on Renal Interstitial Injury in Rats with Chronic Pyelonephritis and its Mechanism. JOURNAL OF CLINICAL RESEARCH, 2022, 39(3): 366-369.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2022.03.013 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2022/V39/I3/366

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