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医学临床研究  2022, Vol. 39 Issue (8): 1135-1137    DOI: 10.3969/j.issn.1671-7171.2022.08.005
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糖尿病视网膜病变模型的构建及评价方法
林新文1, 陈娜2, 陈文明2, 王适3*, 唐闪光4
1.湖南省药品审核查验中心,湖南 长沙 410001;
2.湖南中医药大学第一附属医院,湖南 长沙 410021;
3.湖南中医药大学第二附属医院,湖南 长沙 410005;
4.湖南一格制药有限公司,湖南 湘潭 411100
Construction and Evaluation of Diabetes Retinopathy Model
LIN Xin-wen, CHEN Na, CHEN Wen-ming, et al
Hunan Drug Examination and Inspection Center,Changsha Hunan 410001
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摘要 【目的】探讨链脲佐菌素(STZ)诱导的糖尿病视网膜病变模型的构建及评价方法。【方法】雄性SD大鼠30只,随机分为正常组(10只)、模型组(20只)。模型组腹腔注射STZ(65 mg/kg)3 d后测空腹血糖,选取血糖>14 mmol/L 10只糖尿病大鼠作为模型组。每隔4周称重、检测空腹血糖,采用视网膜光学相干层析成像(OCT)技术观察糖尿病大鼠视网膜血管变化情况,并通过HE染色分析糖尿病大鼠的视网膜组织形态变化。【结果】与正常组比较,模型组大鼠建模3 d后至20周内空腹血糖水平均升高;OCT成像结果显示:模型组大鼠建模第12周时,其眼底血管弯曲,排列散乱;建模第20周时,可观察到明显异常新生血管形成;病理结果显示:糖尿病大鼠第12~20周视网膜内核层结构排列紊乱,神经纤维层和神经节细胞层水肿,管腔扩张,部分细胞核固缩,视网膜神经节细胞数量显著减少。【结论】采用OCT技术动态监测视网膜病变发展过程和检测空腹血糖,可成功评估糖尿病视网膜病变模型。
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关键词 糖尿病视网膜病变大鼠疾病模型, 动物    
Abstract:【Objective】 To establish the model of streptozotocin (STZ)-induced diabetes retinopathy and its evaluation method. 【Methods】 Thirty male SD rats were randomly divided into normal group (10 rats) and model group (20 rats). In the model group, fasting blood glucose was measured 3 days after intraperitoneal injection of STZ (65 mg/kg). Ten diabetes rats with blood glucose >14 mmol/L were selected as the model group. Fasting blood glucose was measured and weighed every 4 weeks. Retinal optical coherence tomography (OCT) was used to observe the changes of retinal blood vessels in diabetes rats, and the morphological changes of retinal tissues in diabetes rats were analyzed by HE staining.【Results】Compared with the normal group, the fasting blood glucose level in the model group increased from 3 days to 20 weeks; OCT imaging results showed that at the 12th week of modeling, the blood vessels in the fundus of the model group were bent and arranged in disorder; At the 20th week of modeling, obvious abnormal neovascularization was observed; The pathological results showed that the retinal nuclear layer of diabetes rats was disorganized from 12 to 20 weeks, the nerve fiber layer and ganglion cell layer were edema, the lumen was dilated, some nuclei were pyknosis, and the number of retinal ganglion cells was significantly reduced.【Conclusion】OCT can be used to dynamically monitor the development of retinopathy and detect fasting blood glucose, which can successfully evaluate the model of diabetes retinopathy.
Key wordsDiabetic Retinopathy    Rats    Disease Models, Animal
收稿日期: 2022-07-21     
中图分类号:  R587.26  
通讯作者: *E-mail:2545411618@qq.com   
引用本文:   
林新文, 陈娜, 陈文明, 王适, 唐闪光. 糖尿病视网膜病变模型的构建及评价方法[J]. 医学临床研究, 2022, 39(8): 1135-1137.
LIN Xin-wen, CHEN Na, CHEN Wen-ming, et al. Construction and Evaluation of Diabetes Retinopathy Model. JOURNAL OF CLINICAL RESEARCH, 2022, 39(8): 1135-1137.
链接本文:  
http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2022.08.005     或     http://journal07.magtech.org.cn/yxlcyj/CN/Y2022/V39/I8/1135
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