IL-35、IL-17在糖尿病视网膜病变患者血清和房水中的表达水平及其意义

doi:10.3969/j.issn.1671-7171.2022.02.028

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摘要【目的】探讨白细胞介素-35(IL-35)、白细胞介素-17(IL-17)在糖尿病视网膜病变(diabetic retinopathy,DR)患者血清和房水中的表达水平及其意义。【方法】选取2019年3月至2021年3月在泾阳县医院行白内障超声乳化手术的DR患者80例(80只眼)为研究对象,另选取同期在泾阳县医院行白内障超声乳化手术的老年白内障患者65例(65只眼)为对照组。比较两组患者临床资料;采用酶联免疫吸附法(ELISA)检测所有患者血清、房水中IL-35、IL-17水平;采用Pearson相关系数分析血清、房水中IL-35、IL-17水平的相关性;采用受试者工作特征(ROC)曲线评价血清、房水中IL-35、IL-17水平及两者联合对DR的诊断价值。【结果】与对照组比较,DR组患者血清、房水中IL-35水平明显降低(P<0.05),IL-17水平均明显升高(P<0.05),且DR患者血清IL-35、IL-17水平低于房水中IL-35、IL-17水平(P<0.05);DR患者血清、房水中IL-35与IL-17水平均呈负相关(P<0.05);血清中IL-35、IL-17水平及两者联合检测对DR诊断的曲线下面积(AUC)分别为0.843、0.863、0.957,两者联合检测诊断DR的AUC明显高于血清中IL-35、IL-17水平的单独诊断(P<0.05);房水中IL-35、IL-17水平及两者联合检测对DR诊断的AUC分别为0.830、0.882、0.963;两者联合检测诊断DR的AUC明显高于房水中IL-35、IL-17水平的单独诊断(P<0.05)。【结论】DR患者血清、房水中IL-35低表达,IL-17高表达,均对DR具有一定诊断作用,但血清标本取材方式简单,更有实用价值。

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	周艾玲
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关键词 ：糖尿病视网膜病变, 糖尿病,2型/并发症, 白细胞介素17/血液

Abstract：【Objective】To detect the levels of interleukin-35 (IL-35) and interleukin-17 (IL-17) in serum and aqueous humor of patients with diabetic retinopathy (DR), and to explore their clinical significance in the pathogenesis of DR. 【Methods】A total of 80 DR patients (80 eyes) who underwent cataract phacoemulsification in our hospital from March 2019 to March 2021 were selected as the study objects, while 65 elderly cataract patients (65 eyes) received cataract phacoemulsification in the same period were selected as the control group. The general clinical data of patients were collected and compared between the two groups. Enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of IL-35 and IL-17 in serum and aqueous humor of subjects. Pearson correlation was used to analyze the correlation of IL-35 and IL-17 in serum and aqueous humor. The diagnostic values of IL-35 and IL-17 in serum and aqueous humor alone and their combination in DR were evaluated by receiver operating characteristic curve (ROC). 【Results】Compared with the control group, the levels of IL-35 in serum and aqueous humor of DR group were significantly lower (P<0.05), while the levels of IL-17 were significantly higher (P<0.05). Furthermore, the levels of IL-35 and IL-17 in serum of DR patients were lower than those in aqueous humor (P<0.05). Results showed that the levels of IL-35 and IL-17 in serum and aqueous humor of DR patients were negatively correlated (P<0.05). The area under curve (AUC) of IL-35, IL-17 and their combination in serum in the diagnosis of DR was 0.843, 0.863 and 0.957 respectively. The AUC of the combined detection of IL-35 and IL-17 in the diagnosis of DR was significantly higher than that of the single diagnosis of IL-35 and IL-17 in the serum (P<0.05). The AUC of IL-35 and IL-17 levels in aqueous humor and their combined detection for the diagnosis of DR was 0.830, 0.882 and 0.963 respectively. The AUC of the combined detection of both IL-35 and IL-17 in the diagnosis of DR was significantly higher than that of the independent diagnosis of IL-35 and IL-17 levels in aqueous humor (P<0.05). 【Conclusion】The expression levels of IL-35 in serum and aqueous humor of DR patients are low, and the expression levels of IL-17 are high in serum and aqueous humor of DR patients, which have certain diagnostic effects on DR. However, the method of serum sample collection is simple and more practical.

Key words： Diabetic Retinopathy Diabetes Mellitus,Type 2/CO Interleukin-17/BL

收稿日期: 2021-12-06

中图分类号:

R587.1

通讯作者: *E-mail:WangChunli666@163.com

引用本文:

周艾玲, 王涵钰. IL-35、IL-17在糖尿病视网膜病变患者血清和房水中的表达水平及其意义[J]. 医学临床研究, 2022, 39(2): 259-262.
ZHOU Ai-ling, WANG Han-yu. Expression and Significance of IL-35 and IL-17 in Serum and Aqueous Humor of Patients with Diabetic Retinopathy. JOURNAL OF CLINICAL RESEARCH, 2022, 39(2): 259-262.

链接本文:

http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2022.02.028 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2022/V39/I2/259

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