Abstract：【Objective】 To explore the effect of alpinia oxyphylla extract on oxidative stress injury of kidney in diabetic nephropathy mice,and to analyze the possible mechanism. 【Methods】Forty SPF male SD mice were randomly divided into the control group,the model group,low and high dose group of alpinia oxyphylla extract (with 10 mice in each group). After establishing diabetic nephropathy mouse model (DN mouse),5 g/(kg?d) and 20 g/(kg?d) of alpinia oxyphylla extract solution with certain amount of normal saline were given to the low and high dose group mice for 4 weeks; while DN mice in the control group was given the same amount of normal saline at the same time period. Hematoxylin-eosin (HE) staining was used to observe the renal histopathology. The levels of 24 h urine protein,serum creatinine and urea nitrogen were determined by automatic biochemical analyzer,the activity of Superoxide dismutase,the content of malondialdehyde (MDA) and the content of glutathione peroxidase (GSH-px) were determined by colorimetry and ELISA,respectively. Nrf2 and HO-1 levels of nuclear factor E2 related factor 2 Heme oxygenase-1(Nrf2/HO-1) were measured by Western blotting. 【Results】Compared to SD mice in the control group,the levels of 24 h urinary protein,urea nitrogen and serum creatinine in the DN model group and mice with the low and high dose groups of alpinia oxyphylla extract increased,while the levels of SOD,GSH-px and Nrf2,HO-1 protein decreased ( P<0.05) . Compared with the DN model group,the levels of 24 h urinary protein,urea nitrogen and serum creatinine decreased in the low-and high-dose groups,and the levels of SOD,GSH-px and Nrf2,HO-1 protein increased ( P<0.05) . Levels of urine protein,urea nitrogen and serum creatinine in mice with the low-dose group of alpinia oxyphylla extract were lower than those in the high-dose group,while the levels of SOD,GSH-px and Nrf2,HO-1 protein were lower ( P<0.05). 【Conclusion】The protective effect of Alpinia Oxyphylla extract on kidney of DN mice may be realized by promoting activation of Nrf2/HO-1 pathway and inhibiting oxidative stress injury of kidney.
任爱霞, 孙艳林, 闫晓红, 王磊. 益智仁提取物对糖尿病肾病小鼠肾氧化应激损伤的影响及机制研究[J]. 医学临床研究, 2022, 39(7): 1029-1032.
REN Ai-xia, SUN Yan-lin, YAN Xiao-hong,et al. Effect and Mechanism of Alpinia Oxyphylla Extract on Oxidative Stress Injury of Kidney in Diabetic Nephropathy Mice. JOURNAL OF CLINICAL RESEARCH, 2022, 39(7): 1029-1032.
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2022, Vol. 39 
