Abstract：【Objective】To investigate protective effect of Shenqi Dihuang decoction on mesangial proliferative glomerulonephritis (MsPGN) rats and its effect on 38 mitogen-activated protein kinase/nuclear transcription factor Kappa B (p38MAPK/NF-κB) pathway. 【Methods】Forty rats were randomly divided into the sham operation group,MsPGN model group and low and high dose groups of shenqi dihuang decoction,with 10 rats in each group. The rats in the sham-operated group were exposed to the left kidney only,and the rest were the same as those in the model group. The rats in the MsPGN group were exposed to the left kidney only after anesthesia,the rats in the low and high dose groups were given 10 mL/(kg·d) of shenqi dihuang decoction (1.4 mg/mL and 2.8 mg/mL solution) for 4 weeks after the MsPGN model was established,rats in the sham operation group and model group were given the same amount of normal saline at the same time. The 24 h urine protein,urea nitrogen and blood creatinine were measured. The levels of IL-8 and TNF-α in renal tissues were detected by ELISA,and p38MAPK/NF-κB pathway proteins (p38MAPK,p-p38MAPK,NF-κB p65) were detected by Western blotting.【Results】Compared with the sham operation group,the levels of 24 h urinary protein,urea nitrogen,serum creatinine,IL-8,TNF-α,p-p38MAPK/p38MAPK and NF-κB p65 in the model group and Shenqi Dihuang decoction group were all increased (P<0.05). Compared with the model group,the levels of 24 h urinary protein,urea nitrogen,serum creatinine,IL-8,TNF-α,p-p38MAPK/p38MAPK and NF-κB p65 in the low and high dose groups were decreased (P<0.05). Compared with the low-dose group,the levels of all indexes in the high dose group were decreased (P<0.05). 【Conclusion】The protective effect of Shenqi Dihuang decoction on MsPGN rats may be realized by inhibiting the activation of p38MAPK/NF-κB pathway and thus inhibiting the inflammatory reaction of renal tissue.
王生辉, 赵强. 参芪地黄汤对系膜增生性肾小球肾炎大鼠的作用及对p38MAPK/NF-κB信号通路的影响[J]. 医学临床研究, 2022, 39(8): 1153-1156.
WANG Sheng-hui, ZHAO Qiang. Protection of Shenqi Dihuang Decoction on Mesangial Proliferative Glomerulonephritis Rats and Its Effect on p38MAPK/NF-κB Signal Pathway. JOURNAL OF CLINICAL RESEARCH, 2022, 39(8): 1153-1156.
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2022, Vol. 39 
