大鼠切口痛模型中经皮穴位电刺激对瑞芬太尼诱发的痛觉过敏的影响

doi:10.3969/j.issn.1671-7171.2022.02.001

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摘要【目的】探讨大鼠切口痛模型中经皮穴位电刺激(Transcutaneous electric acupoint stimulation,TEAS)对瑞芬太尼诱发的术后痛觉过敏的影响。【方法】对所有大鼠进行编号,按随机数表法将其分为三组,即切口痛组、瑞芬太尼组及TEAS治疗组,每组30只。各组分别参照Brennan法建立大鼠切口痛模型。瑞芬太尼组造模成功后术中尾静脉输注瑞芬太尼,TEAS治疗组麻醉后先取同侧足三里穴、三阴交穴电针刺激治疗30 min,电针治疗后尾静脉输注瑞芬太尼。于术前24 h(T₀)及术后6 h(T₁)、24 h(T₂)、48 h(T₃)应用智能热板仪和纤维丝测痛仪检测各组大鼠热刺激缩足反射阈值(PWTL)和机械刺激缩足反射阈值(PMWT),采用免疫组化法对大鼠脊髓组织进行染色,计算神经细胞平均数目,获得c-fos阳性神经细胞的数目。【结果】T₀时,三组大鼠PWTL、PMWT值比较,差异无统计学意义(P>0.05);与T₀时比较,T₁、T₂、T₃时三组大鼠PWTL、PMWT值均呈下降趋势,差异有统计学意义(P<0.05);T₁、T₂、T₃时,瑞芬太尼组、TEAS治疗组大鼠PWTL、PMWT值均高于切口痛组,TEAS治疗组高于瑞芬太尼组,差异均有统计学意义(P<0.05)。大鼠脊髓组织c-fos阳性神经细胞位于胞核,呈深灰褐色、圆形、均一的颗粒,阴性神经细胞为中部深灰色,边缘浅灰色,颜色不均一的颗粒。TEAS治疗组大鼠脊髓组织c-fos阳性神经细胞计数显著低于切口痛组、瑞芬太尼组,差异有统计学意义(P<0.05)。【结论】c-fos可能参与瑞芬太尼诱发切口痛大鼠的术后痛觉过敏,TEAS治疗可延缓切口痛大鼠的术后痛觉过敏。

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关键词 ：疼痛, 经皮神经电刺激, 芬太尼/毒性, 痛觉过敏/化学诱导, 疾病模型,动物, 大鼠

Abstract：【Objective】To investigate the effect of transcutaneous electrical acupoint stimulation (TEAS) on postoperative hyperalgesia induced by remifentanil in rat incision pain model. 【Methods】 All rats were numbered and randomly divided into three groups: incision pain group, remifentanil group and TEAS treatment group, with 30 rats in each group. The rat incision pain model was established by Brennan method in each group. After successful modeling in remifentanil group, remifentanil was infused into caudal vein during operation. After anesthesia in TEAS treatment group, electroacupuncture stimulation at Zusanli and Sanyinjiao on the same side was for 30 minutes, and remifentanil was infused into caudal vein after electroacupuncture treatment. At 24 h (T₀) before operation and 6 h (T₁), 24 h (T₂) and 48 h (T₃) after operation, the thermal stimulation foot contraction reflex threshold (pwtl) and mechanical stimulation foot contraction reflex threshold (pmwt) of rats in each group were measured by intelligent hot plate instrument and filament pain meter. The spinal cord tissue of rats was stained by immunohistochemical method, the average number of nerve cells was calculated, and the number of c-fos positive nerve cells was obtained. 【Results】At T₀, there was no significant difference in terms of pwtl and pmwt values among the three groups (P>0.05). Compared with T₀, pwtl and pmwt in T₁, T₂ and T₃ groups showed a downward trend, and the difference was statistically significant (P<0.05). At T₁, T₂ and T₃, the pwtl and pmwt values of the remifentanil group and the TEAS treatment group were higher than those of the incision pain group, and those values in TEAS treatment group were higher than those in the remifentanil group (P<0.05). The c-fos positive neurons in rat spinal cord were located in the nucleus and they were stained in dark grayish brown, round and uniform granules. The negative neurons were dark gray in the middle and light gray at the edge. The difference in TEAS treatment group was significantly lower than that in the incision pain group and the remifentanil group (P<0.05). 【Conclusion】The c-fos may be involved in postoperative hyperalgesia in rats with incision pain induced by remifentanil. TEAS treatment can delay postoperative hyperalgesia in rats with incision pain.

Key words： Pain Transcutaneous Electric Nerve Stimulation Fentanyl/TO Hyperalgesia/CI Disease Models,Animal Rats

收稿日期: 2021-09-29

中图分类号:

R619.9

基金资助:湖南省科技创新计划项目(S2017FYLJS0165)

引用本文:

张行, 朱立, 李娜, 郑方坤, 刘帆. 大鼠切口痛模型中经皮穴位电刺激对瑞芬太尼诱发的痛觉过敏的影响[J]. 医学临床研究, 2022, 39(2): 161-164.
ZHANG Hang, ZHU Li, LI Na, et al. Effect of Transcutaneous Electrical Acupoint Stimulation on Postoperative Hyperalgesia Induced by Remifentanil in Rat Incision Pain Model. JOURNAL OF CLINICAL RESEARCH, 2022, 39(2): 161-164.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2022.02.001 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2022/V39/I2/161

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