Abstract：【Objective】To investigate the effect of 4-methylumbelliferone (4MU) on monocyte derived dendritic cells (Mo-DCs) in human peripheral blood.【Methods】Human monocytes were obtained by density gradient centrifugation and cultured for 5 days by human granulocyte monocyte colony stimulating factor and interleukin-4, Mo-DCs were treated with different concentrations of 4mu, the activity of Mo-DCs in each group was detected by CCK-8 method, the immunophenotypic marker level of Mo-DCs in each group was detected by flow cytometry, and the IL-6 level of Mo-DCs in each group was detected by electrochemiluminescence method.【Results】 When the concentration of 4mu was 0.1～1 mmol/L, it has little effect on the activity of Mo-DCs. 4MU inhibited the expression of immunophenotypic markers CD40, CD83, CD80, HLA-DR and Mo-DCs secreting IL-6.【Conclusion】 4MU inhibits the maturation of Mo-DCs and reduces the secretion of IL-6, which provides a new idea for the prevention and treatment of infectious inflammation. 4MU may become a new drug for immune regulation.
[1] XU Z,SHI L,WANG Y,et al. Pathological findings of COVID-19 associated with acute respiratory distress syndrome[J].Lancet Respir Med,2020,8(4):420-422. [2] SALLENAVE E M,GUILLOT L. Innate immune signaling and proteolytic pathways in the resolution or exacerbation of SARS-CoV-2 in Covid-19:key therapeutic targets? [J].Front Immunol,2020,28,11:1229. [3] NAGY N,KUIPERS H F,FRYMOYER A R,et al. 4-Methylumbelliferone treatment and hyaluronan inhibition as a therapeutic strategy in inflammation,autoimmunity,and cancer[J].Front Immunol,2015,23,6:123. [4] UGUR M,MUELLER S N.T cell and dendritic cell interactions in lymphoid organs:more than just being in the right place at the right time[J].Immunol Rev,2019,289(1) :115-128. [5] 曹雪涛. 免疫学前沿进展[M]. 北京:人民卫生出版社,2011. [6] TANAKA T,NARAZAKI M,KISHIMOTO T.Immunotherapeutic implications of IL-6 blockade for cytokine storm[J].Immunotherapy,2016,8(8):959-970. [7] COPAESCU A,SMIBERT O,GIBSON A,et al.The role of IL-6 and other mediators in the cytokine storm associated with SARS-CoV-2 infection[J].J Allergy Clin Immunol,2020,146(3):518-534. [8] XU Z,SHI L,WANG Y,et al.Pathological findings of COVID-19 associated with acute respiratory distress syndrome[J].Lancet Respir Med,2020,8(4):420-422. [9] MCKALLIP R J,HAGELE H F,UCHAKINA O N. Treatment with the hyaluronic acid synthesis inhibitor 4-methylumbelliferone suppresses SEB-induced lung inflammation[J].Toxins (Basel),2013,5:1814-1826. [10] LI F,HAO P,LIU G,et al. Effects of 4-methylumbelliferone and high molecular weight hyaluronic acid on the inflammation of corneal stromal cells induced by LPS[J].Graefes Arch Clin Exp Ophthalmol,2017,255(3):559-566.
2022, Vol. 39 
