Abstract：【Objective】 To investigate the changes of gut microbiota in ovariectomized osteoporosis mice fed with Lactobacillus reuteri and Lactobacillus acidophilus.【Methods】 C57BL/6J female mice (n=80) were randomly divided into eight different groups based on treatments: sham surgery (sham), sham with Lactobacillus reuteri feeding, sham with Lactobacillus acidophilus feeding, sham with both Lactobacillus reuteri and Lactobacillus acidophilus co-feeding, OVX surgery (OVX), OVX with Lactobacillus reuteri feeding, OVX with Lactobacillus acidophilus feeding, OVX and both Lactobacillus reuteri and Lactobacillus acidophilus co-feeding. There were 10 mice in each group. Data of 16rRNA high-throughput sequencing were used to analyze and compare the taxonomic composition, alpha diversity index and beta diversity index of gut microbiota in each group. 【Results】 Mice fed with probiotics had changes in the composition of their gut microbiota. Compared to the mice fed without probiotics, Firmicutes and Erysipelotrichia decreased in the OVX with probiotics fed groups. Proteobacteria, gamma proteobacteria and Enterobacterales increased in lactobacillus acidophilus fed groups. Verrucomicrobiota and Akkermansiaceae increased except for group of OVX with lactobacillus acidophilus fed. After the mice were fed with probiotics, the richness of gut microbiota was significantly increased (P<0.05). Ovariectomy made the richness and diversity of gut microbiota decreased significantly (P<0.05).The same surgery modeling and probiotic feeding patterns had similar and regular effects on gut microbiota. Mice fed with probiotics had a greater effect on their gut microbiota than the ovariectomized intervention.【Conclusion】 Lactobacillus reuteri and Lactobacillus acidophilus can change the composition of gut microbiota in ovariectomized mice with osteoporosis. There are certain rules to this change. This can provide experimental basis for studying the relationship of osteoporosis with probiotics and gut microbiota.
陈镜臣, 黎松波, 黎建文, 刘先银. 罗伊乳杆菌及嗜酸乳杆菌对去卵巢骨质疏松小鼠肠道菌群的影响[J]. 医学临床研究, 2022, 39(5): 721-724.
CHEN Jing-chen, LI Song-bo, LI Jian-wen, et al. Effects of Lactobacillus Reuteri and Lactobacillus Acidophilus on Gut Microbiota in Ovariectomized Osteoporosis Mice. JOURNAL OF CLINICAL RESEARCH, 2022, 39(5): 721-724.
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2022, Vol. 39 
