Abstract：【Objective】To investigate the frequency of Treg and levels of Treg-related cytokines (IL-10 and TGF-b) in patients with femoral neck fracture and post-menopausal osteoporosis(PMOP).【Methods】From October 2016 to September 2020, 30 patients with femoral neck fracture and PMOP(the PMOP fracture group),30 patients with femoral neck fracture and normal BMD (in the non-PMOP fracture group,PMOP fracture group),and 30 healthy control subjects (the control group) were enrolled for the study. Chemiluminescence method was used to detect serum osteocalcin (BGP), type Ⅰ collagen carboxy-terminal peptide (b-CTX) and type Ⅰ collagen N-terminal propeptide (PINP). Flow cytometry was used to detect the frequency of Treg cells in each group. The levels of interleukin 10 (IL-10) and transforming growth factor beta (TGF-β) were detected by ELISA.Venous blood was drawn to separate PBMCs and serum, then the frequency of Treg and level of IL-10 and TGF-β was examined and compared among the three groups. The serum level of BGP,PINP and b-CTX were tested and compared as well. 【Results】The frequency of Treg cells and the levels of serum IL-10 and TGF-β in the patients with PMOP fracture were lower than those in the non-PMOP fracture group and the control group (P<0.05). There was no statistical difference between the control group and the non-PMOP fracture group.The serum levels of b-CTX and PINP in the PMOP fracture group were higher than those in the non-PMOP fracture group and the control group, and the BGP level was lower than that in the non-PMOP fracture group and the control group, and the differences were statistically significant (P<0.05). Pearson correlation analysis showed that serum b-CTX and PINP levels were negatively correlated with Treg cell frequency (r=-0.262 and r=-0.255,respectively, both P<0.05).【Conclusion】The frequency of Treg and Treg-related cytokine levels were decreased in patients with femoral neck fracture and PMOP, which might participate in the pathogenesis of osteoporosis.
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