Abstract：【Objective】To investigate the correlation between metabolic indexes and intestinal flora in male patients with non obese non-alcoholic fatty liver disease (NAFLD). 【Methods】 Male patients in the Department of Gastroenterology of the Second Affiliated Hospital of Xinjiang Medical University from January to June 2019 were collected, including 20 cases of non obese NAFLD group (group A), 20 cases of obese NAFLD group (group B), 20 cases of healthy control group (Group C) and 20 cases of normal metabolism obese group (Group D). Hemoglobin (HB), fasting blood glucose (FPG), creatinine (SCR), total cholesterol (TC), triglyceride (TG), short chain fatty acid (SCFA) and inflammatory factor interleukin-17 (IL-17) were detected. 16S rDNA high-throughput sequencing was used to detect cyanobacteria, Bacteroides and Firmicutes in the intestinal flora of group D, and to analyze the correlation between metabolic indexes, inflammatory factors and intestinal flora in male non obese patients with nonalcoholic fatty liver disease. 【Results】 There were significant differences in Hb and TC levels among the four groups (P<0.05); Multivariate logistic regression analysis showed that TC was an independent risk factor for NAFLD in men. The levels of serum SCFA in the group A and the group B were significantly higher than those in the Group C and the Group D. The levels of serum SCFA in the group A were significantly lower than those in the group B (P<0.05); However, there was no significant difference in serum IL-17 levels among the four groups (P>0.05). Intestinal microflora structure of four groups had their own specificity, but Bacteroides, Firmicutes, proteus were the main dominant microflora. The abundance of Bacteroides in the group A was significantly higher than that in the group B, but the abundance of Firmicutes in the group B was significantly lower than that in the group A, the differences were statistically significant (P<0.05). The content of Narcissus was the highest in the group C and the lowest in the group B. There was a negative correlation between cyanobacteria and SCR in the non obese NAFLD group (P<0.05); The abundance of Bacteroides was negatively correlated with IL-17 (P<0.05); The abundance of Firmicutes was negatively correlated with SCFA (P<0.05). There was no significant difference in Firmicutes, Bacteroides, metabolic indexes and inflammatory factors in the obese NAFLD group (P>0.05). 【Conclusion】The structure of intestinal microflora in male non obese NAFLD patients is better than that in obese NAFLD patients. It is speculated that the difference of intestinal dominant bacteria between non obese and obese NAFLD patients may be one of the reasons for their different phenotypes, but the causal relationship between dominant bacteria and different NAFLD phenotypes needs to be further studied.
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