Abstract：【Objective】To investigate the relationship of innate lymphocyte (ILC) and immune factor levels in peripheral blood of patients with activity of lupus nephritis. 【Methods】A total of 94 patients with lupus nephritis admitted to our hospital were divided into the active group (SLEDAI ≥ 10 points, 54 cases) and the stable group (SLEDAI <10 points, 40 cases) according to the systemic lupus erythematosus disease activity index (SLEDAI) score. The healthy subjects in the same period were selected as the control group (40 cases). Flow cytometry was used to detect the percentage of ILC1, ILC2, ILC3 in peripheral blood, and to detect interleukin-33 (IL-33), IL-4, interferon-γ (IFN-γ), IgG, C3 and C4. The correlation among ILC, immune factors (IL-33, IL-4, IFN-γ) and SLEDAI score were analyzed. 【Results】The percentage of ILC1 in the active group was higher, while levels of ILC2 and ILC3 were lower than those in the stable group and the control group (P<0.05).The percentage of ILC1 in the stable group was higher than that of the control group; and percentages of ILC2 and ILC3 in the active group were lower than those in the control group (P<0.05). The levels of C3 and IgG level in the active group were higher than those in the stable group and the control group, while the C4 level in the active group was lower than that in the control group (P<0.05). The level of IL-33 in the active group was lower, and levels of IL-4 and IFN-γ were higher than those in the stable group and the control group (P<0.05). The level of IL-4 in the stable group was lower than that in the control group (P<0.05). Correlation analysis showed that ILC2 was positively correlated with IL-4 (P<0.05) and ILC2 was negatively correlated with SLEDAI score (P<0.05). ILC3 was positively correlated with IL-4 (P<0.05). 【Conclusion】Patients with lupus nephritis have abnormal levels of immunity and ILC, and ILC2 is associated with disease activity.
康云慧, 张星. 狼疮肾炎患者血固有淋巴细胞、免疫因子水平与疾病活动的关系[J]. 医学临床研究, 2021, 38(8): 1218-1220.
KANG Yun-hui, ZHANG Xing. Lymphocyte and Immune Factor Levels in Patients with Lupus Nephritis and Relationship with Disease Progression. JOURNAL OF CLINICAL RESEARCH, 2021, 38(8): 1218-1220.
[1] ALMAANI S, MEARA A, ROVIN B H, et al. Update on Lupus Nephritis[J].Clin J Am Soc Nephrol,2017,12(5):825-835. [2] LORENZ G,DESAI J,ANDERS H J,et al. Lupus nephritis: update on mechanisms of systemic autoimmunity and kidney immunopathology[J].Curr Opin Nephrol Ypertens,2014,23(3):211-217. [3] LORENZ G, ANDERS H J.Neutrophils, dendritic cells, toll-like receptors, and interferon-α in lupus nephritis[J].Semin Nephrol,2015,35(5):410-426. [4] RUIZ-SÁNCHEZ B P, CRUZ-ZÁRATE D, ESTRADA-GARCÁA I,et al.Innate lymphoid cells and their role in immune response regulation[J].Rev Alerg Mex,2017,64(3):347-363. [5] MORETTA L, LOCATELLI F.Innate lymphoid cells in normal and disease: An introductory overview[J].Immunol Lett,2016,179:1. [6] 顾镭,王慧娟,张缪佳, 等.B细胞与系统性红斑狼疮的发生发展及治疗[J].中华风湿病学杂志,2007,11(5):312-315. [7] 储荟,方璇,谭震, 等.系统性红斑狼疮外周血固有淋巴细胞变化与其临床相关性[J].中华医学杂志,2019,99(3):169-173. [8] 平利峰,王晓磊,孙凤艳, 等.狼疮性肾炎患者血清自身免疫抗体水平变化及相关性研究[J].疑难病杂志,2019,18(3):289-292. [9] FUCHS A, VERMI W, LEE J S,et al.Intraepithelial type 1 innate lymphoid cells are a unique subset of IL-12- and IL-15-responsive IFN-γ-producing cells[J].Immunity,2013,38(4):769-781. [10] GUO C, ZHOU M, ZHAO S,et al.Innate lymphoid cell disturbance with increase in ILC1 in systemic lupus erythematosus[J].Clin Immunol,2019,202:49-58. [11] BAL S M, BERNINK J H, NAGASAWA M,et al.IL-1β, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs[J].Nat Immunol,2016,17(6):636-645.
2021, Vol. 38 
