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Quick Search     Adv Search 2021 Vol. 38, No. 6 Published: 2021-06-26   806 Comparison of the Diagnostic Value of Serum Chitinase 3-like Protein 1 Level and Liver Elasticity in the Diagnosis of Liver Fibrosis MAO Zhuo, LIAO Ai-jun, LIAO Jin-mao, et al DOI: 10.3969/j.issn.1671-7171.2021.06.002 【Objective】To explore the diagnostic value of the serum chitinase 3-like protein 1 (CHI3L1) level and Fibroscan in patients with liver fibrosis. 【Methods】A total of 392 outpatients and inpatients in the Department of Liver Diseases of Hunan Provincial People's Hospital from September 2018 to December 2019 were collected. Fibroscan was used to measure liver elasticity (LS). LS value ≤7.3 kPa was included in the non-fibrosis group (n=115), LS value ≤17.4 kPa was included in the fibrosis group (n=85), and LS value ≥17.5 kPa was included in the cirrhosis group group (n=192).Enzyme-linked immunosorbent assay (ELISA) was used to detect serum CHI3L1 levels in all subjects, an automatic biochemical analyzer was used to determine patient liver function indexes (ALT, AST, TBIL, DBIL, TP, ALB), and Fibroscan was used to determine liver stiffness (LS). The correlation between CHI3L1 and liver function indexes was analyzed and the ROC curve of serum CHI3L1 level and LS value in the diagnosis of liver fibrosis patients was drawn.【Results】The clinical data of the three groups of patients were compared, and the difference of age, BMI, combined drinking history were statistically significant (P<0.05). The CHI3L1, LS, liver function indexes (ALT, AST, TBIL, DBIL, TP, ALB) of each group were compared, and the differences were statistically significant (P<0.05). The level of CHI3L1 in the non-fibrosis group was not correlated with various liver function indexes and LS values (P>0.05). The level of serum CHI3L1 in the fibrosis group was not correlated with various liver function indexes (P>0.05), but positively correlated with LS (P<0.05). Serum CHI3L1 level in the cirrhosis group was positively correlated with ALT and LS, negatively correlated with ALB (P<0.05), and not correlated with AST, TBIL, DBIL, TP (P>0.05). The area under curve of the serum CHI3L1 and LS value are 0.841 and 0.717, respectively. Both methods have high diagnostic efficiency for patients with liver fibrosis, and the diagnostic efficiency of serum CHI3L1 is slightly higher than the LS value. 【Conclusion】Serum CHI3L1 levels are closely related to liver function indexes such as ALT and ALB, and can reflect early liver damage. Compared with Fibroscan, it has higher diagnostic efficiency in patients with liver fibrosis and is worthy of clinical promotion. 2021 Vol. 38 (6): 806-810 [Abstract] ( 204 ) HTML (1 KB)  PDF (1182 KB)  ( 89 ) 811 Expression and Clinical Significance of DOCK5 in Cervical Cancer Based on Bioinformatics Analysis XIAO Yu-zhen, ZHAO Li-yun, XIA Xiao-meng DOI: 10.3969/j.issn.1671-7171.2021.06.003 【Objective】The Dedicator of Cytokinesis (DOCKs) family, containing 11 members, is a class of proteins with guanine nucleotide exchange factor activity and plays an important role in the processes of life. However, the expressions and clinical values of DOCKs in cervical squamous cells remain unknown. Based on bioinformatics analysis, this article aims to fully reveal the expression pattern and clinical significance of DOCKs in cervical squamous cell carcinoma.【Methods】UACLAN, GEPIA, Kaplan-Meier Plotter, cBioPortal and TIMER online databases were used to analyze the expression levels, gene mutations, prognostic value of DOCKs genes in cervical squamous cell carcinoma and their correlation with immune cell infiltration.【Results】Compared with normal tissues, the expression of DOCK5 was significantly upregulated and the expression of DOCK3/11 was downregulated in cervical squamous cell carcinoma (P<0.05), respectively. The expression of DOCK5 was significantly different among distinct tissue types of cervical squamous cell carcinoma. DOCK5 expression was significantly negatively correlated with overall survival (OS) and relapse free survival (RFS) in patients with cervical squamous cell carcinoma (P<0.05). The gene mutation rate of DOCK5 was 6%, and the OS and RFS time of patients with DOCK5 mutation were notably shorter (P<0.05). DOCK5 expression was negatively correlated with B cell and macrophage infiltration (P<0.05), positively associated with B cell marker CD19, neutrophil marker KIR2DL1, helper Th2 cell marker STAT6, helper Th17 cell marker STAT3 and the expression level of NK cell marker NCR1 (P<0.05).【Conclusion】DOCK5 expression was upregulated in cervical squamous cell carcinoma, which was positively correlated with poor prognosis of patients, and was related to the regulation of tumor immune microenvironment in patients with cervical squamous cell carcinoma, suggesting that DOCK5 is involved and plays vital roles in the occurrence, development and prognosis of cervical squamous cell carcinoma. 2021 Vol. 38 (6): 811-816 [Abstract] ( 124 ) HTML (1 KB)  PDF (1859 KB)  ( 231 ) 817 Correlation between Infertility Pressure and Cortisol Level in Women with In Vitro Fertilization ZHOU Peng-fei, WANG Cheng-xiu DOI: 10.3969/j.issn.1671-7171.2021.06.004 【Objective】To investigate the correlation between infertility pressure and cortisol level (COR) in women undergoing in vitro fertilization(IVF).【Methods】During June 2016 and January 2018, 237 couples who received IVF or intracytoplasmic sperm injection (ICSI) in our reproductive center were included in the study. Fertility Problem Inventory (FPI) was used to measure the infertility pressure of the subjects. Serum cortisol levels were measured by chemiluminescence assay. Pearson's correlation was used to compare the correlation between infertility pressure and serum cortisol level. Logistic regression was used to analyze the risk factors of pregnancy outcome in IVF patients.【Results】The average age of 237 women was (33.8±4.3) years old. The BMI was (24.14±3.17) kg/m2, and the infertility time was (2.73±1.14) years. The infertility pressure score of IVF patients was (183.47±24.27) points, and the serum COR level was (4.73±0.26) ng/mL. The correlation analysis showed that infertility pressure score was positively correlated with serum cortisol level ( P< 0.05, with Pearson's correlation coefficient r=0.878). The total fertilization rate of 237 infertile patients undergoing IVF treatment was 65.71%, and the clinical pregnancy rate was 51.24%. Multivariate logistic regression analysis showed that aged≥30 years old, smoking, alcohol consumption, high infertility pressure, and high serum cortisol level were independent risk factors for in vitro fertilization and clinical pregnancy failure (P< 0.05). 【Conclusion】Women receiving IVF treatment have higher infertility pressure and cortisol levels. Fertilization rate and clinical pregnancy rate decreased with the increase of infertility pressure and cortisol level. 2021 Vol. 38 (6): 817-819 [Abstract] ( 108 ) HTML (1 KB)  PDF (1227 KB)  ( 31 ) 820 Clinical Efficacy of Ginkgo Diterpenoid Lactone Meglumine Injection Combined with Alteplase Intravenous Thrombolysis in the Treatment of Elderly Patients with Acute Ischemic Stroke XU Hui, ZHANG Xiao-guang, YUE Yun-hua, et al DOI: 10.3969/j.issn.1671-7171.2021.06.005 【Objective】To investigate the clinical efficacy of Ginkgo diterpenoid lactone meglumine injection (DGMI) combined with alteplase (rt-PA) intravenous thrombolysis in the treatment of elderly patients with acute ischemic stroke (AIS). 【Methods】 From January 2018 to August 2020, 86 elderly patients with AIS in Shanghai Yangpu District Central Hospital were selected as the research objects, and the observation group and the control group were randomly divided into two groups, 43 cases in each group. The control group was treated with rt-PA intravenous thrombolysis, and the observation group was treated with DGMI on the basis of the control group for 2 weeks. The clinical efficacy, neurological function, activities of daily living, serum levels of amyloid A (SAA), lipoprotein associated phospholipase A2 (Lp-PLA2) and hemorheological parameters were compared between the two groups before and after treatment. 【Results】The total effective rate of the observation group was 93.02% (40/43), significantly higher than 74.42% (32/43) of the control group, the difference was statistically significant(χ2=5.460,P=0.019). After treatment, the clinical neurological deficit score (NDS), serum SAA, Lp-PLA2 levels, high shear and low shear whole blood viscosity and plasma viscosity of the two groups were significantly lower than those of the same group before treatment (mean P<0.05), and the observation group was significantly lower than that of the control group (mean P<0.05); After treatment, ADL of the two groups was significantly higher than that of the same group before treatment (mean P<0.05), and the observation group was significantly higher than that of the control group (P<0.05). 【Conclusion】 DGMI combined with rt-PA intravenous thrombolysis in the treatment of elderly patients with AIS has significant curative effect, can effectively improve the hemorheology of patients, significantly reduce the serum SAA, Lp-PLA2 levels, reduce the degree of neurological deficit, and improve the ability of daily living, it is worthy of clinical application. 2021 Vol. 38 (6): 820-823 [Abstract] ( 171 ) HTML (1 KB)  PDF (1139 KB)  ( 111 ) 824 Effect of Minimally Invasive Stereotactic Soft Channel Hematoma Drainage on Postoperative Neurological Function and Serum Inflammatory Factors in Patients with Hypertensive Basal Ganglia Hemorrhage FENG Le-xiao, SU Yong-yong, XIE Jiang-tao, et al DOI: 10.3969/j.issn.1671-7171.2021.06.006 【Objective】To investigate the effect of minimally invasive stereotactic soft channel hematoma drainage on postoperative neurological function and serum inflammatory factors in patients with hypertensive basal ganglia hemorrhage. 【Methods】The clinical data of 77 patients with hypertensive basal ganglia hemorrhage admitted to our hospital from June 2018 to June 2020 were retrospectively analyzed. According to the operation scheme, they were divided into traditional group (traditional craniotomy, n=38) and minimally invasive group (minimally invasive stereotactic soft channel hematoma drainage, n=39). The hematoma clearance rate and complications were compared between the two groups, TNF- α, Procalcitonin (PCT), nerve function (NIHSS) and activity of daily living (ADL) were measured.【Results】The hematoma clearance rate of minimally invasive group was better than that of traditional group, and the difference is statistically significant(χ2=1.835,P=0.033＜0.05). One week and one month after operation, TNF -αin the two groups, the PCT level was lower than that before operation, and the minimally invasive group was lower than that of the traditional group, and the difference was statistically significant (P<0.05). The difference of NIHSS and ADL scores in minimally invasive group was higher than that of the traditional group (P<0.05). The incidence of complications in minimally invasive group was 5.12% (2/39), which was lower than that of the traditional group (23.68% (9/38), and the difference was statistically significant(χ2=5.412,P=0.02＜0.05).【Conclusion】Minimally invasive stereotactic soft channel hematoma drainage in patients with hypertensive basal ganglia hemorrhage can reduce the level of serum inflammatory factors, improve the neurological function of patients, and is conducive to the prognosis of patients. 2021 Vol. 38 (6): 824-826 [Abstract] ( 93 ) HTML (1 KB)  PDF (1113 KB)  ( 42 ) 827 Diagnostic Value of Venography and Ultrasonography in Recurrence of Varicose Veins of Lower Extremity LIU Peng, LIU Chong, ZHENG Li-hua, et al DOI: 10.3969/j.issn.1671-7171.2021.06.007 【Objective】To explore the application value of MSCTV combined with ultrasound in the analysis of the causes of recurrence of varicose veins of lower extremity. 【Methods】 From June 2017 to June 2019, 52 patients with postoperative recurrence of varicose veins of lower extremities in our hospital were selected. The clinical data of the patients were retrospectively analyzed, and the influencing factors of postoperative recurrence of varicose veins were analyzed. 【Results】 There was no significant difference in recurrence years between patients undergoing conventional exfoliation, intraoperative thermal ablation and foam sclerotherapy (P>0.05). Compared with venography, MSCTV had a higher detection rate of iliac vein compression (IVCS) and could identify the cause of compression. IVCS (18/54) was the primary cause of postoperative recurrence of varicose veins of lower extremities. BMI was negatively correlated with the recurrence time of varicose veins (P<0.05). The recurrence years of patients with family history of varicose veins, obesity and without pressure treatment were higher than those without family history of varicose veins, obesity and pressure treatment (P<0.05). The recurrence years of IVCS and RVR patients were higher than those of non IVCS and RVR patients (P<0.05). 【Conclusion】The combined application of MSCTV and color Doppler ultrasound can accurately diagnose the causes of postoperative recurrence of varicose veins of lower extremities. 2021 Vol. 38 (6): 827-830 [Abstract] ( 101 ) HTML (1 KB)  PDF (1667 KB)  ( 92 ) 831 Clinical Significance of Salivary Helicobacter Pylori (Hp) Antibody IgG and Fecal Hp Antigen Detection in Patients with Chronic Gastritis ZHANG Xue-yan, FAN Jie DOI: 10.3969/j.issn.1671-7171.2021.06.008 【Objective】To reveal the clinical significance of helicobacter pylori (Hp) antibody IgG (HpIgG) detection in saliva and Hp antigen in feces of patients with chronic gastritis. 【Methods】The patients with chronic gastritis who were treated in our hospital were selected as the observation group and healthy adults who had physical examination in our hospital during the same period were selected as the control group. The results of HpIgG in saliva and Hp antigen in feces of the two groups were observed. The differences of pepsinogen (PGⅠ and PGⅡ), G-17 (gastrin 17), cytokine hypersensitive C-reaction protein(hs-CRP) and cytokines (IL-18 and IL-6) levels between the two groups were compared. The correlation between the results of HpIgG in saliva and Hp antigen in feces of patients with chronic gastritis and the levels of PGⅠ, PGⅡ, G-17 and cytokines was analyzed. 【Results】In the observation group, the positive rates of HpIgG in saliva and Hp antigen in feces were 72.00% and 45.00%, respectively, which were higher than those in the control group (P<0.01). The levels of PGⅠ and PGⅡ in the observation group were lower than those in the control group, while the level of G-17 in the observation group was higher than that in the control group (P<0.01). The levels of IL-18, IL-6 and hs-CRP in the observation group were higher than those in the control group (P<0.01). The positive rates of HpIgG in saliva and Hp antigen in feces of patients in the observation group were negatively correlated with the levels of PGⅠ and PGⅡ, and positively correlated with the levels of G-17, IL-18, IL-6 and hs-CRP (P<0.05). 【Conclusion】The positive rate of HpIgG in saliva and Hp antigen in feces is higher in patients with chronic gastritis, and it is related to pepsin and cytokine levels. 2021 Vol. 38 (6): 831-833 [Abstract] ( 82 ) HTML (1 KB)  PDF (1118 KB)  ( 64 ) 834 Relationship between Spine Related Parameters, Clinical Characteristics of Senile Osteoporosis and Risk of Osteoporotic Fracture in the Elderly LIU Kai, ZHANG Xiao-jun, DING Tao DOI: 10.3969/j.issn.1671-7171.2021.06.009 【Objective】To investigate the relationship between spine related parameters, clinical characteristics of senile osteoporosis (SOP) and the risk of osteoporotic fracture. 【Methods】A total82 cases of elderly of patients in our hospital from April 2018 to April 2020 were selected as the research objects (observation group); In addition, 82 patients with SOP in our hospital during the same period were selected as the control group. Before treatment, the basic data of gender, age, body mass index (BMI), residential area, smoking, drinking, outdoor sports, family fracture history, previous fracture history, education level, bone mineral density, and spinal parameters [thoracic kyphosis angle (TK), thoracolumbar kyphosis angle (TLK), lumbar lordosis angle (LL)] were collected, ROC was used to analyze the value of bone mineral density, TK, TLK and LL in predicting of of patients with SOP, and then the difference information between the two groups was included in the multivariate logistic regression analysis for quantitative evaluation, so as to determine the risk factors of of of patients with SOP. 【Results】 There was no significant difference in gender, living area, family fracture history and education level between the two groups (P>0.05); The proportion of patients with age ≥ 75 years old, BMI < 18.5 kg/m2, smoking, drinking, no outdoor exercise and previous fracture history in the observation group was significantly higher than that in the control group (P<0.05); The levels of TK and TLK in the observation group were significantly higher than those in the control group, and the levels of bone mineral density and LL in the observation group were significantly lower than those in the control group (P<0.05). ROC analysis confirmed that bone mineral density, TK, TLK and LL could be used to predict of in patients with SOP, AUC were 0.716, 0.882, 0.863 and 0.831 respectively (mean P<0.05). Multivariate logistic regression analysis confirmed that age ≥ 75 years old, BMI < 18.5 kg/m2, smoking, drinking, no outdoor sports, previous history of fracture, BMD ≤ 0.695 g/cm2, TK ≥ 33.550 °,TLK≥23.985 °and LL≤31.450°. It was the risk factor of patients with SOP (P<0.05).【Conclusion】 There are many risk factors for of in patients with SOP, among which TK, TLK and LL are the important risk factors, and clinicians should pay close attention to them. 2021 Vol. 38 (6): 834-837 [Abstract] ( 69 ) HTML (1 KB)  PDF (1221 KB)  ( 36 ) 838 Clinical Study of Ultrasound-guided Small Needle Knife in the Treatment of Cervical Shoulder Myofascial Pain Syndrome HU Wen-wen, FU Zhi-yuan, LIU Liang, et al DOI: 10.3969/j.issn.1671-7171.2021.06.010 【Objective】To explore the efficacy and safety of ultrasound-guided small needle knife in the treatment of myofascial pain syndrome (MPS). 【Methods】From December 2019 to January 2021, 73 patients diagnosed as MPS in the shoulder pain clinic of Hunan Provincial People's Hospital (the First Affiliated Hospital of Hunan Normal University) with neck and shoulder pain were prospectively collected. The subjects were randomly divided into experimental group (n=37) and control group (n=36). The experimental group was treated with small needle knife guided by visual ultrasound, and the control group was treated with traditional needle knife; The gray-scale ultrasonographic manifestations of myofascial trigger points (MTrPs), Young's modulus values of MTrPs and adjacent areas before and after treatment, visual analogue scale (VAS) before, during and after treatment of the two groups were recorded respectively, and whether there were needle syncope, pneumothorax, bleeding, pain, etc. in the whole treatment process of the two groups were recorded Nerve damage and other adverse events occurred. 【Results】The characteristics of MTrPs in gray-scale sonogram were mainly low echo area, and the young's modulus of MTrPs was higher than that of adjacent areas (P<0.05). The VAS of the two groups during and after treatment were lower than before treatment; The young's modulus after treatment was lower than that before treatment (P<0.05). There was no significant difference in VAS and Young's modulus before treatment between the two groups (P>0.05), but the VAS and Young's modulus after treatment in the experimental group were significantly lower than those in the control group (P<0.05). Pearson correlation analysis showed that MTrPs and Young's modulus were positively correlated with VAS (r=0.8, P<0.01). There were 3 cases of adverse events in the control group, which were manifested as the formation of local hematoma in the treatment area. There was no obvious bleeding in the experimental group, and there were no adverse events such as needle syncope, pneumothorax and nerve damage in the two groups. 【Conclusion】Compared with the traditional small needle knife, ultrasound-guided small needle knife in the treatment of neck and shoulder MPs has better curative effect and higher safety, which is worthy of clinical application and promotion. 2021 Vol. 38 (6): 838-842 [Abstract] ( 94 ) HTML (1 KB)  PDF (1508 KB)  ( 277 ) 843 Effects of Single Port Laparoscopic Surgery on Benign Ovarian Tumor WANG Yong-mei, LI Hong-mei DOI: 10.3969/j.issn.1671-7171.2021.06.011 【Objective】analyze the effect of single port laparoscopic surgery on benign ovarian tumor. 【Methods】The clinical data of 68 patients with benign ovarian tumor admitted to our hospital from May 2016 to December 2018 were analyzed retrospectively. Patients were divided into two groups with 34 cases each: the control group was treated with porous laparoscopic surgery, the observation group was treated with single-hole (single port) laparoscopic surgery, and the clinical efficacy of the two groups was analyzed. 【Results】The postoperative exhaust time (13.20±1.33) h hospitalization time (2.70±1.46) d in the observation group was shorter than that in the control group (P<0.05); and the operative time (63.28±8.03) min was longer than that in the control group (P<0.05). However, the intraoperative blood loss and postoperative feeding time were not statistically significant (P>0.05). Preoperatively, there were no significant differences in the levels of ovarian function indexes, inflammatory factors indexes [C-reactive protein (CRP), tumor necrosis factor-α (TNF-α)], β-endorphin (β-ep), cortisol (Cor), human epididymal protein 4 (HE4) and follistatin (FS), (P>0.05). The level of TNF- α, CRP, β-ep, Cor, HE4 and FS in the observation group was lower than that in the control group (P<0.05), but the level of estradiol (E2), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) was not statistically significant compared to that in the control group (P>0.05).There were 4 cases of abnormal menstruation in the observation group, with an incidence of 11.76% (4/34), and 12 cases of abnormal menstruation in the control group, with an incidence of 35.29% (12/34); comparison of abnormal menstruation rates in the two groups showed statistically significant difference (P<0.05). 【Conclusion】Single-port laparoscopic surgery for benign ovarian tumor can reduce postoperative exhaust time, reduce the level of inflammatory factors in patients, improve the menstrual status of patients, and does not have a significant impact on ovarian function. 2021 Vol. 38 (6): 843-845 [Abstract] ( 93 ) HTML (1 KB)  PDF (1117 KB)  ( 42 ) 846 SATB1, NF-κB and HER-2 Expression Patterns in Invasive Ductal Breast Carcinoma and Their Correlation with Clinical and Pathological Parameters WANG Wen-zhong, LI Yu-hui, TAN Bing DOI: 10.3969/j.issn.1671-7171.2021.06.012 【Objective】To investigate the expression patterns of Special AT-rich binding protein 1(SATB1), nuclear factor-kappa B p65(NF-κB p65) and HER-2 in invasive ductal breast carcinoma, and their correlation with the clinical and pathological Parameters.【Methods】The protein expression of SATB1, NF-κB p65 and HER-2 were examined by immunohistochemistry, and fluorescence in situ hybridization (FISH) was employed to detect HER2 amplification. 【Results】The expression rates of SATB1, NF-κB p65 and HER-2 in 90 cases of invasive ductal breast carcinoma were 71%(64/90), 53.3%(48/90) and 30%(27/90) respectively, and higher in comparison to normal breast tissues. The expression of SATB1 and NF-κB p65 were correlated with tumor size, histological classification, TNM stage, lymph node metastasis and ER status, and not associated with age. The over-expression of HER-2 was only correlated with lymph node metastasis, but not associated with tumor size, histological classification, TNM stage, ER status and age. The positive correlation was found between the expression of SATB1 and NF-κB, but HER2 expression was not significantly correlated with that of other proteins. 【Conclusion】The expression of SATB1 and NF-κB were closely correlated with clinical and pathological parameters of invasive ductal breast carcinoma, and may have synergistic effect in during the occurrence and development of breast carcinoma. The expression of SATB1 and NF-κB may be used as prognostic biomarkers of invasive ductal breast carcinoma. 2021 Vol. 38 (6): 846-850 [Abstract] ( 83 ) HTML (1 KB)  PDF (1747 KB)  ( 35 ) 851 Study on the Correlation of Serum Endogenous Growth Hormone Releasing Peptide and Oxidative Stress Media in Patients with Acute Pancreatitis PAN Rong, DOU Zhen-feng DOI: 10.3969/j.issn.1671-7171.2021.06.013 【Objective】To detect the changes of serum endogenous growth hormone releasing peptide (Ghrelin), paraoxonase-1 (PON-1), superoxide dismutase (SOD) and malondialdehyde (MDA) in patients with acute pancreatitis (AP). 【Methods】A total of 195 patients with AP were selected as the research object (observation group), including 106 mild cases (mild group), 59 moderate cases (moderate group) and 30 severe cases (severe group). Another 45 healthy people were selected as control group. On the day of physical examination, venous blood samples were collected from patients with AP on D1, D3 and D7 for detection of endogenous Ghrelin, PON-1, SOD and MDA. The changes of the above indexes and the correlation between endogenous Ghrelin and PON-1, SOD and MDA were analyzed. 【Results】 The levels of serum endogenous Ghrelin, PON-1 and SOD in AP patients gradually decreased with the remission of the disease, and were lower than those in the control group, while the level of serum MDA was just the opposite, and the comparison among the groups was statistically significant (P<0.05). With the extension of admission time, the serum endogenous Ghrelin, PON-1 and SOD levels of AP patients gradually increased with the improvement of the disease, while the serum MDA level gradually decreased, with statistical significance at different time points in the same group (P<0.05). Spearman correlation showed that serum endogenous Ghrelin was positively correlated with PON-1 and SOD (r=0.335 and 0.414, P<0.001), and negatively correlated with MDA (r=-0.393, P<0.001). 【Conclusion】The level of serum endogenous Ghrelin decreases with the aggravation of AP, and is related to the oxidative stress mediators such as PON-1, SOD and MDA, which provides a basis for the early diagnosis of AP, the judgment of disease severity and the search for new therapeutic targets. 2021 Vol. 38 (6): 851-854 [Abstract] ( 77 ) HTML (1 KB)  PDF (1254 KB)  ( 36 ) 855 Correlation Analysis of Metabolic Abnormality and Intestinal Flora in Male Non-obese Non-alcoholic Fatty Liver Disease Patients LIANG Can-can, YAO Ping DOI: 10.3969/j.issn.1671-7171.2021.06.014 【Objective】To investigate the correlation between metabolic indexes and intestinal flora in male patients with non obese non-alcoholic fatty liver disease (NAFLD). 【Methods】 Male patients in the Department of Gastroenterology of the Second Affiliated Hospital of Xinjiang Medical University from January to June 2019 were collected, including 20 cases of non obese NAFLD group (group A), 20 cases of obese NAFLD group (group B), 20 cases of healthy control group (Group C) and 20 cases of normal metabolism obese group (Group D). Hemoglobin (HB), fasting blood glucose (FPG), creatinine (SCR), total cholesterol (TC), triglyceride (TG), short chain fatty acid (SCFA) and inflammatory factor interleukin-17 (IL-17) were detected. 16S rDNA high-throughput sequencing was used to detect cyanobacteria, Bacteroides and Firmicutes in the intestinal flora of group D, and to analyze the correlation between metabolic indexes, inflammatory factors and intestinal flora in male non obese patients with nonalcoholic fatty liver disease. 【Results】 There were significant differences in Hb and TC levels among the four groups (P<0.05); Multivariate logistic regression analysis showed that TC was an independent risk factor for NAFLD in men. The levels of serum SCFA in the group A and the group B were significantly higher than those in the Group C and the Group D. The levels of serum SCFA in the group A were significantly lower than those in the group B (P<0.05); However, there was no significant difference in serum IL-17 levels among the four groups (P>0.05). Intestinal microflora structure of four groups had their own specificity, but Bacteroides, Firmicutes, proteus were the main dominant microflora. The abundance of Bacteroides in the group A was significantly higher than that in the group B, but the abundance of Firmicutes in the group B was significantly lower than that in the group A, the differences were statistically significant (P<0.05). The content of Narcissus was the highest in the group C and the lowest in the group B. There was a negative correlation between cyanobacteria and SCR in the non obese NAFLD group (P<0.05); The abundance of Bacteroides was negatively correlated with IL-17 (P<0.05); The abundance of Firmicutes was negatively correlated with SCFA (P<0.05). There was no significant difference in Firmicutes, Bacteroides, metabolic indexes and inflammatory factors in the obese NAFLD group (P>0.05). 【Conclusion】The structure of intestinal microflora in male non obese NAFLD patients is better than that in obese NAFLD patients. It is speculated that the difference of intestinal dominant bacteria between non obese and obese NAFLD patients may be one of the reasons for their different phenotypes, but the causal relationship between dominant bacteria and different NAFLD phenotypes needs to be further studied. 2021 Vol. 38 (6): 855-858 [Abstract] ( 71 ) HTML (1 KB)  PDF (1138 KB)  ( 33 ) 859 Application of Nurse-led Multidisciplinary Pulmonary Rehabilitation Intervention in Perioperative Period of Lung Cancer Patients YAO Xiao-hua, PAN Rui-lan, YUAN Jin-quan, et al DOI: 10.3969/j.issn.1671-7171.2021.06.015 【Objective】To explore the value of nurse-led multidisciplinary pulmonary rehabilitation intervention in the perioperative period of lung cancer patients. 【Methods】Eighty lung cancer patients admitted to the thoracic surgery department of Dongguan People's Hospital from July 2018 to July 2019 were randomly divided into experimental group (40 cases) and control group (40 cases). In the experimental group, nurse-led multidisciplinary pulmonary rehabilitation comprehensive intervention measures were adopted on the basis of routine postoperative nursing, including preoperative respiratory function exercise, early respiratory function exercise and early out of bed activities. The changes of related indexes were collected. 【Results】In the experimental group, the time of bed activity, the average time of detaining the chest tube and the time of detaining the urinary tube were significantly shortened, In addition, the pulmonary function and the arterial oxygen pressure of the first to fifth days were significantly better than those of the control group. 【Conclusion】nurse-led multidisciplinary pulmonary rehabilitation intervention can rapidly and effectively improve the preoperative pulmonary function reserve, promote postoperative pulmonary rehabilitation, reduce the incidence of postoperative complications, and improve the quality of nursing. 2021 Vol. 38 (6): 859-862 [Abstract] ( 79 ) HTML (1 KB)  PDF (1151 KB)  ( 227 ) 863 Application Effect of Nano Carbon Tracer Technology in Radical Operation of Colon Cancer and its Influence on Long-term Survival Outcome of Patients WEN Peng-hao, LI Xian-zhe, XIAO Dan, et al DOI: 10.3969/j.issn.1671-7171.2021.06.016 【Objective】To explore the application effect of nano-carbon tracer technology in radical operation of colon cancer and its influence on long-term survival outcome of patients. 【Methods】 A total of 128 patients with colon cancer treated by laparoscopic radical operation in our hospital were selected as the research object, 58 patients were treated with lymph node dissection under nano-carbon tracer (observation group), and 70 patients were treated with routine lymph node dissection (control group). The operation process index, lymph node dissection index, 3-year survival rate and survival time were compared between the two groups. 【Results】The operation time and blood loss of the observation group were significantly lower than those of the control group (P<0.05); There was no significant difference in postoperative intestinal exhaust time and hospital stay between the two groups (P>0.05); The number of lymph nodes and positive lymph node detection rate in the observation group were higher than those in the control group, and the difference was statistically significant (P<0.05). The incidence of postoperative complications in the observation group was 8.62% (5/58), which was significantly lower than 12.86% (9/70) in the control group (χ2=0.584,P=0.445>0.05). There were no significant differences in the lost follow-up rate and 3-year survival rate between the observation group and the control group (P>0.05); The total survival time of the observation group was 30.0 months, which was significantly higher than that of the control group (25.0 months, P<0.05).【Conclusion】 Nano-carbon tracer technology in radical operation of colon cancer is helpful to reduce the difficulty of operation, can better clean lymph nodes, and has certain significance for prolonging the survival time of patients. 2021 Vol. 38 (6): 863-865 [Abstract] ( 79 ) HTML (1 KB)  PDF (1125 KB)  ( 36 ) 866 Effect of Labor Process Input Management in Painless Delivery on Maternal Coagulation Function and Pregnancy Outcome YANG Jing, YANG Li DOI: 10.3969/j.issn.1671-7171.2021.06.017 【Objective】To explore the effect of labor process input management in painless delivery on maternal coagulation function and pregnancy outcome.【Methods】 From May 2018 to may 2020,a total of 140 parturients who delivered in our hospital were selected as the research objects and randomly divided into observation group and control group, with 70 cases in each group. The control group received routine epidural anesthesia and diet according to the wishes of the puerpera, while the observation group received input management on the basis of the control group. The time of the first stage of labor, the second stage of labor, the third stage of labor, the amount of bleeding during labor, the interval of uterine contraction, the duration of uterine contraction, fetal heart rate and the incidence of adverse pregnancy outcomes and sensory score before and after delivery (LAS, VAS, VAS-A), coagulation function index (D-D, TT, FIB) were compared between the two groups.【Results】The second stage of labor time, the third stage of labor time, intrapartum blood loss, uterine contraction interval time in the observation group were significantly less than those in the control group, the fetal heart rate was significantly higher than that in the control group, the differences were statistically significant (P<0.05). The VAS-A score of the observation group after delivery was significantly lower than that before delivery and after delivery of the control group, the difference was statistically significant (P<0.05). FIB in the observation group after delivery was significantly lower than that before delivery, and higher than that in the control group, the difference was statistically significant (P<0.05). The total incidence of adverse pregnancy outcomes in the observation group was 12.86% (9/70), which was significantly lower than 24.29% (17/70) in the control group (χ2=4.464,P=0.035).【Conclusion】The application of labor process input management in painless delivery can significantly shorten the time of labor process, improve coagulation function index, and reduce the incidence of adverse pregnancy, which is worthy of clinical application. 2021 Vol. 38 (6): 866-869 [Abstract] ( 66 ) HTML (1 KB)  PDF (1136 KB)  ( 97 ) 870 Correlation Analysis of BPV, Heart Rate Variability and Acute Kidney Injury in Patients with Essential Hypertension and STEMI LIU Xiu-min DOI: 10.3969/j.issn.1671-7171.2021.06.018 【Objective】To investigate the relationship between blood pressure variability (BPV), heart rate variability(HRV) and acute kidney injury (AKI) in patients with essential hypertension and ST segment elevation myocardial infarction (STEMI). 【Methods】A total of 100 patients with primary hypertension complicated with STEMI in our hospital were selected as the research objects, and they were divided into AKI group (n=23) and non AKI group (n=77) according to whether AKI occurred during hospitalization. The 24 h systolic blood pressure variability (24hSBPR), 24 h diastolic blood pressure variability (24hDBPV), standard deviation of 24 h normal ECG RR interval (SDNN) and percentage of 24 h adjacent RR interval difference > 50 ms in total RR interval (PMN50) were compared between the two groups. The correlation between BPV, heart rate variability and AKI in patients with essential hypertension and STEMI was analyzed by logistic regression analysis. 【Results】There was no significant difference in age, gender, BMI, smoking, drinking, diabetes and dyslipidemia between the group AKI and the non AKI group (P>0.05). The SCR level at admission and the time from onset to emergency in the AKI group were higher than those in the non AKI group (P<0.05). The 24hSBPV and 24hDBPV of the AKI group were higher than those of the non AKI group, and the SDNN and PMN50 of the AKI group were lower than those of the non AKI group, the differences were statistically significant (mean P<0.05). The increase of SCR level, 24hSBPV and 24hDBPV at admission, and the prolonged time from onset to emergency were the independent risk factors for AKI in patients with essential hypertension and STEMI (P<0.05); There was no correlation between SDNN, PMN50 and AKI in essential hypertension patients with STEMI (P>0.05). 【Conclusion】 The increase of BPV in patients with essential hypertension and STEMI may lead to AKI. Early monitoring and active intervention are needed for these patients. 2021 Vol. 38 (6): 870-872 [Abstract] ( 71 ) HTML (1 KB)  PDF (1119 KB)  ( 59 ) 873 Clinical Effect of Terazosin Combined with Tamsulosin in the Treatment of Benign Prostatic Hyperplasia and its Impact of the Level on Serum PSA and TNF- α XU Juan, LI Xiao-xue DOI: 10.3969/j.issn.1671-7171.2021.06.019 【Objective】To investigate the clinical effect of terazosin combined with tamsulosin on patients with benign prostatic hyperplasia and its effect on the level of the impact of serum PSA and TNF-α. 【Methods】 The clinical data of 102 patients with benign prostatic hyperplasia in our hospital from December 2017 to April 2019 were retrospectively analyzed. According to different treatment schemes, they were divided into observation group (n=50) and control group (n=52). The control group was treated with terazosin, and the observation group was treated with tamsulosin on the basis of the control group. The clinical effect, serum prostate specific antigen (PSA) and tumor necrosis factor-α (TNF)-α were compared between the two groups. The maximum urine flow, residual urine volume and International Prostate Symptom Score (IPSS) were recorded. 【Results】The total effective rate of the observation group was 92.0% (46/50), significantly higher than 86.5% (45/52) of the control group, the difference was statistically significant(χ2=2.8319,P=0.004<0.05).After treatment, the level of serum TNF- αand PSA of the observation group was significantly higher than that of the control group, there was significant difference between the two groups (P<0.05). After treatment, the maximum urine flow and IPSS score of the observation group were significantly higher than those of the control group, and the residual urine volume of the observation group was significantly lower than that of the control group (P<0.05).【Conclusion】 Terazosin combined with tamsulosin can effectively improve the maximum urine flow and residual urine volume of patients with benign prostatic hyperplasia, reduce the level of PSA, and improve the anti-inflammatory immune effect, which is worthy of clinical application. 2021 Vol. 38 (6): 873-875 [Abstract] ( 115 ) HTML (1 KB)  PDF (1115 KB)  ( 29 ) 876 Effect of Dexmedetomidine Combined with Ketorolac Tromethamine on Preventing Pain in Patients undergoing Laparoscopic Cholecystectomy ZHU Ming-xia, HE Hua-li DOI: 10.3969/j.issn.1671-7171.2021.06.020 【Objective】To investigate the analgesic effect of dexmedetomidine combined with ketorolac tromethamine for laparoscopic cholecystectomy (LC). 【Methods】A total of 120 patients undergoing laparoscopic cholecystectomy were enrolled and divided into 4 groups according to the random number table: the dexmedetomidine group, the ketorolac tromethamine group, the combination group (dexmedetomidine combined with ketorolac Triamcinol) and the control group (saline group), with 30 cases in each group. The pain level (VAS score), sedation (Ramsay score) and stress indicators of the 4 groups were recorded. 【Results】The amount of remifentanil in the combination group was lower than that in the other three groups (P<0.05). At the moment of deflation and extubation, the levels of norepinephrine, cortisol and blood glucose in the combination group were lower than those of the other three groups (P<0.05), while the SOD level in the combination group was higher than that of the other three groups (P<0.05). At 1, 4, 6, 12 and 24h after extubation, the VAS scores in the combination group were lower than those in the other three groups (P<0.05). The Ramsay score in the combination group at 1 and 4 h after extubation was higher than that in the control group, and lower than that in the dexmedetomidine group and ketorolac tromethamine group (P<0.05). 【Conclusion】The application of dexmedetomidine combined with ketorolac tromethamine in patients undergoing laparoscopic cholecystectomy can reduce the use of opioids while it has a good analgesic effect. 2021 Vol. 38 (6): 876-878 [Abstract] ( 76 ) HTML (1 KB)  PDF (1112 KB)  ( 29 ) 879 Effect of Enhanced External Counterpulsation Combined with Rosuvastatin on Prognosis of Patients with Acute Myocardial Infarction Undergoing PCI HAN Wen-he, SUN Ya-lan, ZHANG Shou-ben DOI: 10.3969/j.issn.1671-7171.2021.06.021 【Objective】To investigate the effect of enhanced external counterpulsation combined with rosuvastatin on the prognosis of patients with acute myocardial infarction (AMI) undergoing percutaneous coronary intervention (PCI).【Methods】From April 2018 to June 2020, 116 AMI patients who underwent PCI in our hospital were prospectively selected and randomly divided into observation group and control group, 58 cases in each group. The control group was treated with rosuvastatin after PCI, and the observation group was treated with enhanced external counterpulsation on the basis of the control group for 1 month. The curative effects of the two groups were compared with the indexes of cardiac function [left ventricular ejection fraction (LVEF), left ventricular end diastolic volume (LVEDV), left ventricular end systolic volume (LVESV)], hemorheological indexes (whole blood high shear viscosity, whole blood low shear viscosity, erythrocyte sedimentation rate, fibrinogen), serum vascular endothelial function indexes [nitric oxide, Endothelin-1 (ET-1) and von Willebrand factor (vWF)] before and 1 month after operation were measured. The incidence of major adverse cardiovascular events (MACE) 1 month after operation was recorded.【Results】The total effective rate of observation group was 91.38% (53/58), which was higher than that of the control group [75.86% (44/58], and the difference was statistically significant(χ2=5.098,P=0.024＜0.05). The LVEF level of the two groups was higher than that of the control group one month after operation, and the LVEDV and LVESV levels in the observation group were lower than that of the control group (P<0.05); The whole blood viscosity, whole blood low shear viscosity, erythrocyte sedimentation rate and fibrinogen were lower in the two groups than that in the control group (P<0.05); The serum NO level of the two groups was higher than that of the control group, ET-1 and vWF were lower than that of the control group (P<0.05); The incidence of mace in the observation group was 6.90% (4/58), which was lower than that of the control group (22.41% (13/58), and the difference was statistically significant(χ2=5.583,P=0.018＜0.05).【Conclusion】Enhanced external counterpulsation combined with rosuvastatin has significant effect in patients after AMI PCI, which can improve the cardiac function and reduce the risk of mace. 2021 Vol. 38 (6): 879-882 [Abstract] ( 70 ) HTML (1 KB)  PDF (1143 KB)  ( 33 ) 883 Efficacy of Apatinib Mesylate Tablets in the Treatment of Advanced Gastric Cancer LIU Fu, MA Pei, WEI Hai DOI: 10.3969/j.issn.1671-7171.2021.06.022 【Objective】To observe the efficacy of apatinib mesylate tablets in the treatment of advanced gastric cancer and to provide reference for clinical treatment. 【Methods】A total of 70 patients with advanced gastric cancer after first-line chemotherapy (FOLFOX regimen) were randomly divided into two groups. The control group was treated with tigio and the observation group was treated with apatinib mesylate tablets. The clinical efficacy of the two groups after treatment was analyzed. 【Results】In the observation group, there was 1 case of complete remission and 15 cases of partial remission. The objective remission rate was 45.71%. There were 0 cases of complete remission in the control group and 14 cases of partial remission. The objective remission rate was 40.00% (14/35). There was no significant difference in the objective remission rate between the two groups (P>0.05). There was no significant difference in the levels of interferon-gamma (INF- γ), IL-4 (IL-4), serum sugar chain antigen 19- 9 (CA19-9), and sugar chain antigen 72 -4 (CA72-4) between the two groups before treatment (P>0.05). After treatment, the levels of INF- γ and IL-4, CA19-9, CA72-4 in the two groups were higher than those before treatment, and the levels of INF- γ and IL-4, CA19-9, and CA72-4 in the observation group were better than those in the control group after treatment (P<0.05). The incidence of hypertension (22.86%) was higher in the observation group than in t the control group (5.71%), the incidence of diarrhea (11.43%) was lower than that in the control group (31.43%), and the incidence of nausea and vomiting (8.57%), hand and foot syndrome (2.86%), and neutropenia (0.00%) were lower than those of the control group (11.43%, 5.71%, 5.71%, respectively). The difference was statistically significant (P<0.05). 【Conclusion】Apatinib mesylate tablets and tigio tablets have similar efficacy in the treatment of advanced gastric cancer. Apatinib mesylate tablets have more obvious improvement on related cytokines, but attention should be paid to the occurrence of hypertension. 2021 Vol. 38 (6): 883-885 [Abstract] ( 71 ) HTML (1 KB)  PDF (1116 KB)  ( 47 ) 886 Influence of Pathogenic Bacteria Distribution on Fracture Healing in Patients with Open Tibial Fracture Wound Infection ZHANG Hua, LU Qian, QIAO Rong-fang DOI: 10.3969/j.issn.1671-7171.2021.06.023 【Objective】To explore the influence of pathogenic bacteria distribution on fracture healing in patients with open tibial fracture wound infection. 【Methods】From June 2016 to June 2019, 92 patients with open tibial fracture wound infection were selected as the research objects. The pathogenic bacteria of the infected exudates were identified, and the corresponding internal fixation treatment was taken according to the patients' own situation. All the patients were followed up 6 months after operation, and the factors that may affect the fracture healing were collected and analyzed. The factors that may affect the fracture healing were analyzed by logistic regression analysis. 【Results】A total of 119 strains of pathogenic bacteria were isolated from 92 patients with open tibial fracture. Among them, 79 strains were Gram-negative bacteria, mainly Escherichia coli, accounting for 40.34%; 40 strains were Gram-positive bacteria, mainly Staphylococcus aureus, accounting for 16.81%. All 92 cases were followed up for 6 months, 69 cases healed, the rate of fracture healing was 75.00%. The single factor analysis showed that the factors influencing fracture healing were related to age, fracture cause, fracture type, time from injury to treatment and time of soft tissue repair (P<0.05). Multivariate logistic regression analysis showed that: age ≥60 years old, high-energy injury, fracture type III, fracture type II, injury to treatment time ≥ 6 h and secondary repair of soft tissue were independent risk factors (OR=2.72, 3.36, 3.37, 1.26, 2.16, 2.37, P<0.05). 【Conclusion】Escherichia coli is the main pathogen of open tibial fracture wound infection. In addition, age ≥ 60 years old, high-energy injury, high fracture classification, time from injury to treatment ≥ 6h and secondary repair of soft tissue are independent risk factors for fracture healing in patients with open tibial fracture wound infection. Pre-measures should be taken to reduce the risk of delayed wound healing and promote the early recovery of patients. 2021 Vol. 38 (6): 886-889 [Abstract] ( 64 ) HTML (1 KB)  PDF (1131 KB)  ( 44 ) 890 Effect of Xingnaojing Combined with Edaravone on the Levels of VEGF, BNP and Copeptin in Patients with Cerebral Hemorrhage JIA Nan-jie, FENG Jun-jie DOI: 10.3969/j.issn.1671-7171.2021.06.024 【Objective】To analyze the effect of Xingnaojing combined with edaravone on the levels of vascular endothelial growth factor (VEGF), brain natriuretic peptide (BNP) and copeptin in patients with cerebral hemorrhage. 【Methods】A total of 96 patients with cerebral hemorrhage were selected and divided into the control group and the observation group according to different treatment methods. Both groups were given conventional treatments. Patients in the control group were treated with Xingnaojing alone, while patients in the observation group were treated with Xingnaojing assisted by edaravone. The clinical efficacy of the two groups were analyzed after treatment.【Results】The total effective rate of patients in the observation group was 95.83% (46/48), which was higher than that of the control group 75.00% (36/48, P<0.05). There was no significant difference in the levels of indicators between the two groups before treatment (P>0.05). After treatment, the levels of ET, VEGF, BNP, copeptin, SF, NO, and MDA in the two groups were lower than those before treatment; and the above indicators in the observation group were lower than those in the control group after treatment. The difference was statistically significant (P<0.05). Before treatment, there was no statistically significant difference between the two groups in terms of of NIHSS, MoCA (the Montreal cognitive assessment scale), and ADL (activity of daily living scale) scores (P>0.05). After treatment, the NIHSS scores of the two groups were lower than those before treatment, and MoCA and ADL scores were higher than those before treatment. The NIHSS, MoCA and ADL scores of the observation group after treatment were better than those of the control group (P<0.05). 【Conclusion】Xingnaojing combined with edaravone therapy can improve the efficacy of cerebral hemorrhage, reduce the levels of BNF, copeptin, SF, NO, and MDA in patients, and improve the neurological function of patients. It is worthy of clinical application. 2021 Vol. 38 (6): 890-892 [Abstract] ( 56 ) HTML (1 KB)  PDF (1115 KB)  ( 31 ) 893 Effect of GnRH-α combined with Guizhi Fuling Pill on Ovarian Reserve Function in Patients with Ovarian Endometriosis Cyst after Laparoscopic Surgery WU Lin, DENG Mao-fang DOI: 10.3969/j.issn.1671-7171.2021.06.025 【Objective】To investigate the effect of gonadotropin releasing hormone agonist (GnRH-α) combined with Guizhi Fuling pills on the improvement of basic hormones and ovarian reserve function in patients with ovarian endometriosis cysts after laparoscopy. 【Methods】A total of patients with endometriosis cysts treated in our hospital from January 2017 to December 2018 were selected as the subjects. The patients in each group were randomly divided into control group (n=53) and observation group (n=53). Both groups received laparoscopic surgery. The control group was treated with GnRH-α after operation while the observation group was treated with GnRH-α combined with Guizhi Fuling Pill after operation. The course of treatment in both groups was 6 months. The basic hormones, ovarian reserve function, dysmenorrhea, sexual intercourse pain, pelvic pain and other Kupperman scores and VAS scores were compared between the two groups before and after treatment, and the clinical efficacy of the two groups was observed. 【Results】The total effective rate in the observation group was higher than that in the control group (92.45% vs73.58%, P<0.05). There was no significant difference in the expression of basic hormones between the two groups before treatment (P>0.05). After 6 months of treatment, the expression of follicular stimulating hormone (FSH) in two groups was increased while the expression of E2 and LH (LH) were decreased with statistical significance (P<0.05). The expression of E2,LH and FSH in the observation group was significantly lower than that in the control group (P<0.05). There was no significant difference in ovarian reserve function between the two groups before treatment(P>0.05). After 6 months of treatment, the expression of anti-Muller tube hormone (AMH) decreased, the number of ovarian basal sinus follicles increased, and the peak (PSV) of ovarian stroma artery blood flow did not change significantly (P>0.05). There was no significant difference in the number of AFC in the control group after treatment (P>0.05), The levels of AMH and PSV were significantly decreased (P<0.05). Before treatment, there was no significant difference in VAS score between the two groups in dysmenorrhea, sexual intercourse pain and pelvic pain. After 6 months of treatment, the VAS scores of the two groups were lower than those before treatment, the difference was statistically significant (P<0.05). After treatment, the VAS scores of the observation group were lower than those of the control group (P<0.05). 【Conclusion】GnRH-α combined with Guizhi Fuling Pill is effective in improving laparoscopic surgery in patients with ovarian Endometriosis cysts. It can regulate the level of sex hormones, improve ovarian reserve function.It is worthy of clinical application. 2021 Vol. 38 (6): 893-896 [Abstract] ( 63 ) HTML (1 KB)  PDF (1140 KB)  ( 63 ) 897 Correlation between Mean Platelet Volume, Red Blood Cell Distribution Width and Systemic Lupus Erythematosus Activity Index Score ZHANG Mo-yin, QIN Shu-fang, LAI Yan-jun DOI: 10.3969/j.issn.1671-7171.2021.06.026 【Objective】To investigate the correlation between mean platelet volume (MPV), red blood cell distribution width (RDW) and systemic lupus erythematosus activity index score (SLEDAI). 【Methods】According to SLEDAI score, 120 SLE patients were divided into active group and inactive group. 50 healthy volunteers in the same period were selected as the control group. The correlation between MPV and RDW and SLEDAI score was analyzed. ROC curve was used to evaluate the value of MPV and RDW in predicting patients' activity.【Results】(1) RDW, MPV, ESR, CRP and IgG in active group were significantly higher than those in inactive group and control group (P<0.05); HGB, C3 and C4 levels in active group were significantly lower than those in inactive group and control group (P<0.05). The levels of RDW, MPV, ESR, CRP and IgG in the inactive group were significantly higher than those in the control group (P<0.05); the levels of HGB, C3 and C4 in the inactive group were significantly lower than those in the control group (P<0.05). (2) Pearson correlation analysis showed that SLEDAI score was positively correlated with MPV and RDW (P<0.05). (3) The AUC and 95%CI of MPV were 0.594 and 0.468-0.697, respectively. When the cutoff point was 12.98 fL, the sensitivity and specificity were 56.9% and 77.4%, respectively.The prediction of RDW level shows that the AUC and 95%CI of activity were 0.687 and (0.417-0.783), respectively. When the cutoff point was 14.23%, the sensitivity and specificity were 67.8% and 86.7%, respectively.【Conclusion】 MPV and RDW have potential clinical value in assessing SLE disease activity. 2021 Vol. 38 (6): 897-899 [Abstract] ( 86 ) HTML (1 KB)  PDF (1116 KB)  ( 257 ) 900 Relationship of ALC1 and PLR-3 Expression with Pathological Features of Esophageal Cancer ZHAO Min, SUN Feng-ling, XUE Niu-yan DOI: 10.3969/j.issn.1671-7171.2021.06.027 【Objective】To investigate the relationship of ALC1 (amplification of liver cancer protein) and PLR-3 (protein tyrosine phosphatase-3) expression with the pathological features of esophageal cancer. 【Methods】Eighty cases of esophageal cancer tissue specimens obtained by surgical treatment in our hospital and 40 specimens of adjacent paracancerous tissues were selected. Western blotting analysis was used to detect the relative expression levels of ALC1 protein and PLR-3 protein in the two groups. The expression intensities of ALC1 protein and PLR-3 protein in esophageal carcinoma tissues with different TNM stage, lymph node metastasis, tumor differentiation degree and depth of invasion were analyzed. 【Results】The relative expression levels of ALC1 protein and PLR3 protein in esophageal carcinoma were significantly higher than those in adjacent tissues (P<0.05). The relative expression intensity of ALC1 protein in esophageal carcinoma tissues with different TNM staging and lymph node metastasis did show differently. The difference was statistically significant (P<0.05). The relative expression intensity of PLR3 protein in esophageal carcinoma tissues with lymph node metastasis was statistically significant difference from that in esophageal cancer tissues without lymph node metastasis (P<0.05). 【Conclusion】The expression of ALC1 and PLR-3 in esophageal cancer tissues was significantly up-regulated and closely related to the occurrence and development of tumors. 2021 Vol. 38 (6): 900-902 [Abstract] ( 69 ) HTML (1 KB)  PDF (1239 KB)  ( 37 ) 903 Expression and Clinical Significance of PSA and P504S in Prostate Cancer SONG Ya-dong, LI Ping DOI: 10.3969/j.issn.1671-7171.2021.06.028 【Objective】To investigate the expression and clinical significance of PSA and P504S in prostate cancer. 【Methods】 A total of 62 cases of prostate cancer and 32 cases of benign prostatic hyperplasia from May 2018 to May 2020 were selected for the study. The expression of P504S and PSA in prostate cancer tissues was detected by immunohistochemistry. The relationship of PSA and P504S expression with clinical pathological characteristics was analyzed. 【Results】The positive expression rates of P504S and PSA in prostate cancer were 93.55% (58/62) and 90.32% (56/62), which were significantly higher than their 0% (0/32) and 3.13% (1 /32), and the differences between the two groups were significant (all P<0.05). The positive expression of P504S and PSA in prostate cancer and benign prostatic hyperplasia was significantly different (P<0.05).The positive expression rate of P504S and PSA in prostate cancer increased with the increase of pathological grade, and the difference was statistically significant (P<0.05).In prostate cancer, the expression of P504S and PSA in high stage was significantly higher than that in low stage (P<0.05). The expression of PSA decreased in prostate cancer patients with higher Gleason score. While the expression of P504S increased with higher Gleason score. The difference was statistically significant (P<0.05). 【Conclusion】The expression levels of P504S and PSA can properly reflect the biological behavior of prostate cancer. 2021 Vol. 38 (6): 903-905 [Abstract] ( 71 ) HTML (1 KB)  PDF (1116 KB)  ( 24 ) 906 Analysis of Risk Factors of Perioperative Ischemic Stroke in Patients with Cerebrovascular Stenosis Treated by Stent Implantation DU Fang-lei, CAO Hong-bin DOI: 10.3969/j.issn.1671-7171.2021.06.029 【Objective】To investigate the risk factors of perioperative ischemic stroke in patients with cerebrovascular stenosis treated by stent implantation. 【Methods】The clinical data of 160 patients with cerebrovascular stenosis treated by stent implantation in our hospital from May 2015 to may 2019 were retrospectively analyzed. According to the occurrence of perioperative ischemic stroke, they were divided into non stroke group (n=139) and stroke group (n=21). The general information, NIHSS before and after operation, the use of vasoactive drugs before and after treatment, imaging examination before and after operation, the mean value of systolic blood pressure (SBP) in the morning and afternoon 1 day before operation, and the slowest value of heart rate (HR) were recorded. Logistic multivariate analysis was used to analyze the risk factors of perioperative ischemic stroke.【Results】 Among 160 patients, 21 (13.13%) had ischemic stroke after stent implantation. There were significant differences between the two groups in average age, antiplatelet drugs before admission, calcified plaque, preoperative combined with untreated vascular stenosis, and the slowest HR < 60 beats/min (P<0.05). Increased age, calcified plaque and preoperative combined with untreated vascular stenosis were independent risk factors for perioperative ischemic stroke in patients with stent implantation cerebral vascular stenosis (P<0.001). The use of antiplatelet drugs before admission and the slowest HR value during operation were protective factors (P<0.001). 【Conclusion】The older age, calcified plaque and preoperative combined with untreated vascular stenosis are independent risk factors of perioperative ischemic stroke in patients with stent implantation cerebral vascular stenosis, and a safe surgical treatment plan should be formulated according to individual differences of patients before operation. 2021 Vol. 38 (6): 906-908 [Abstract] ( 74 ) HTML (1 KB)  PDF (1114 KB)  ( 40 ) 909 Clinical Application value of MSCT in Diagnosis of Left Atrial Thrombosis YANG Xiao-jun, SUN Hong-tao DOI: 10.3969/j.issn.1671-7171.2021.06.030 【Objective】To explore the clinical value of multi-slice spiral CT (MSCT) in the diagnosis of left atrial thrombosis. 【Methods】A total of 163 patients with atrial fibrillation admitted to our hospital were enrolled in the study. Among them, 38 patients were diagnosed with atrial thrombosis by esophageal echocardiography (TEE). All patients underwent MSCT examination. The TEE results were used as the gold standard. The sensitivity, specificity, missed diagnosis rate, misdiagnosis rate and consistency kappa value of the diagnosis of left atrial thrombosis by MSCT were calculated. 【Results】A total of 163 patients with atrial fibrillation were enrolled in the MSCT scan. Among them, 62 patients had filling defects in the first phase scan, 57 patients had filling defects in the second phase scan; There were 53 patients who were diagnosed as filling defects in both first and second phase scans, of which 44 cases of intracavitary thrombosis and 9 cases of left atrial appendage thrombus were diagnosed. A total of 163 patients with atrial fibrillation underwent MSCT scan and 53 cases were diagnosed as atrial thrombosis. Of which, 36 cases were completely consistent with TEE. Among 108 patients who were diagnosed with no left atrial thrombus, only 2 cases were missed. The sensitivity of MSCT scan for diagnosis of left atrial thrombosis was 94.74%, specificity was 86.40%, missed diagnosis rate was 5.26%, misdiagnosis rate was 13.60%, and the kappa value was 0.713 with TEE examination. 【Conclusion】MSCT has a high consistency in the diagnosis of left atrial thrombosis and TEE, and its non-innovative characteristics have high application value in clinical. 2021 Vol. 38 (6): 909-911 [Abstract] ( 56 ) HTML (1 KB)  PDF (1112 KB)  ( 33 ) 912 Relationship of Serum IL-38, sTREM-1, and IL-6 Levels with Status of Chronic Obstructive Pulmonary Disease LU Sha-sha, DUAN Yong-bo, SONG Yu, et al DOI: 10.3969/j.issn.1671-7171.2021.06.031 【Objective】To investigate the changes of serum interleukin-38 (IL-38), interleukin-6 (IL-6) and soluble myeloid receptor-1 (sTREM-1) in patients with chronic obstructive pulmonary disease (COPD) and their significance. 【Methods】A total of 100 patients with COPD were selected as the COPD group while 100 healthy volunteers without lung disease were selected as the control group. Serum IL-38, sTREM-1, and IL-6 levels were measured in both groups. According to COPD condition, patients were divided into a stable phase group and an acute stratification group. 【Results】The levels of serum IL-38, sTREM-1, and IL-6 in the COPD group were significantly higher than those in the control group (P<0.05). Serum IL-38, sTREM-1, IL-6 levels of patients in the acute stratification group were significantly higher than those of patients in the stable phase group (P<0.05). FEV1%pred (the forced expiratory volume in the first second as a percentage of the predicted value) and FEV1/FVC (forced vital capacity) in patients with acute exacerbation of COPD were lower than that in stable phase, with statistically significant difference (P<0.05). The CAT score of the acute stratification group was higher than that of the stable phase group (P<0.05). The CAT score of COPD patients was positively correlated with IL-38, sTREM-1 and IL-6 levels (P<0.05). 【Conclusion】The levels of serum IL-38, sTREM-1, and IL-6 in patients with COPD increased significantly and were related to the severity of the disease. 2021 Vol. 38 (6): 912-914 [Abstract] ( 70 ) HTML (1 KB)  PDF (1115 KB)  ( 45 ) 915 Clinical effect of Modified Swenson Operation Assisted by Laparoscopy in the Treatment of Hirschsprung's Disease in Children LI Yan-li DOI: 10.3969/j.issn.1671-7171.2021.06.032 【Objective】To investigate the short-term and long-term effects of modified Swenson and Soave operation assisted by laparoscopy in the treatment of short segment Hirschsprung's disease in children. 【Methods】 Eighty children with short segment Hirschsprung's disease who underwent modified Swenson operation or Soave operation assisted by laparoscope were selected and divided into Swenson group and Soave group, 40 cases in each group. The operation indexes, short-term postoperative recovery and complications were compared between the two groups. The quality of life score, anal function score and the incidence of long-term complications were observed. 【Results】There was no significant difference in the length of bowel resection, indwelling catheter time and eating time between the two groups (P>0.05); The intraoperative blood loss, operation time and postoperative defecation frequency of the Swenson group were lower than those of the Soave group, and the postoperative intestinal peristalsis recovery time and postoperative hospital stay of Swenson group were shorter than those of the Soave group (P<0.05). There was no significant difference in the incidence of perianal dermatitis, anastomotic leakage, pelvic infection, enterocolitis and urinary retention between the two groups in 1 months after operation (P>0.05). The incidence of constipation, fecal contamination and anastomotic stenosis in the Swenson group was lower than that in the Soave group (P<0.05). After 2 years of follow-up, there was no significant difference in the incidence of enterocolitis, constipation and fecal contamination between the two groups (P>0.05); The excellent and good rate of quality of life and anal function in the two groups were higher, and the difference was not statistically significant (P>0.05). 【Conclusion】In the treatment of short segment Hirschsprung's disease in children, laparoscopic modified Swenson operation has more advantages than Soave operation in the incidence of short-term complications, postoperative recovery and intraoperative bleeding. 2021 Vol. 38 (6): 915-917 [Abstract] ( 76 ) HTML (1 KB)  PDF (1118 KB)  ( 32 ) 918 Expression and significance of Nrf2 in endometrioid adenocarcinoma GENG Wen-jing, WU You-min, WANG Jin-sheng, et al DOI: 10.3969/j.issn.1671-7171.2021.06.033 【Objective】To investigate the expression and significance of Nrf2 in endometrioid adenocarcinoma. 【Methods】From January 2012 to December 2015, 5 cases of normal endometrium, 12 cases of endometrial hyperplasia, 11 cases of endometrial atypical hyperplasia and 26 cases of endometrioid adenocarcinoma were collected in the Department of pathology of our hospital. The expression level of Nrf2 in each tissue was detected by immunohistochemical method, and its clinical significance was analyzed. 【Results】 The high expression rate of Nrf2 in endometrioid adenocarcinoma, endometrial hyperplasia, atypical hyperplasia and normal endometrium was 88.5% (23/26), 66.7% (8/12), 63.6% (7/11) and 40% (2/5), respectively. The high expression rate of Nrf2 in endometrioid adenocarcinoma was significantly higher than that in normal endometrium, and the difference was statistically significant(χ2=6.31,P<0.05); There was no significant difference in the high expression rate between the other groups (mean P>0.05). The high expression rate of Nrf2 was not related to the age, stage, grade, tumor thrombus in vessels and the depth of myometrial invasion (mean P>0.05), but related to lymph node metastasis (P<0.05). 【Conclusion】 Nrf2 is overexpressed in endometrioid adenocarcinoma, which may play a role in promoting the occurrence, development and metastasis of endometrioid adenocarcinoma. 2021 Vol. 38 (6): 918-920 [Abstract] ( 65 ) HTML (1 KB)  PDF (1667 KB)  ( 29 ) 921 Observation on the Dosage of Propofol in Abdominal Operation of Patients with Different Degrees of Hepatitis B Cirrhosis BAI Wei-Jie DOI: 10.3969/j.issn.1671-7171.2021.06.034 【Objective】To investigate the dosage of propofol in esophagogastric fundus venous devascularization in patients with different degrees of hepatitis B cirrhosis.【Methods】 A total of 93 patients with hepatitis B cirrhosis who received esophagogastric fundus venous devascularization in our hospital from November 2018 to November 2019 were included in the study. All patients were divided into group A (n=36), group B (n=30) and group C (n=27) according to Child-Pugh liver function grading. In addition, 30 cases of abdominal surgery without hepatitis B cirrhosis admitted to our hospital during the same period were included in the study and set as the control group. All patients in the four groups received anesthesia induction of propofol, and the concentration of ventricular effect, target concentration and usage of propofol when the four groups lost consciousness and the BIS was 50 were compared, as well as the occurrence of hemodynamic events in the four groups.【Results】 Compared to the control group, there were statistically significant differences in the concentration, target concentration and dosage of propofol in the four groups (P<0.05). The comparison of propofol effect concentration, target concentration and usage amount at the moment of lost consciousness and BIS reaching 50 showed that the control group>A group>B group>C group, and the differences showing a decreasing trend among the groups were all significant(P<0.05). The incidence of hypotension, arrhythmia and tachycardia in group C was significantly higher than those in control group and group A, and the difference was statistically significant(P<0.05).【Conclusion】 In patients with hepatitis B cirrhosis, with the aggravation of cirrhosis, the dosage of propofol is also significantly reduced, and the incidence of hypotension, arrhythmia and tachycardia is also significantly increased 2021 Vol. 38 (6): 921-922 [Abstract] ( 56 ) HTML (1 KB)  PDF (1096 KB)  ( 21 )

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