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Quick Search     Adv Search 2015 Vol. 32, No. 10 Published: 2015-10-26   1873 Experiment Study of Construction of Animal Plateau Frostbite Models of  Different Degree Hot! Experiment Study of Construction of Animal Plateau Frostbite Models of  Different Degree[J]. 医学临床研究, 2015,32(10): 1873-1878')" href="#"> JIAO Mingke, LOU Lin，GENG Xiliang，et al 【Objective】 To construct the animal plateau frostbite (PF) models . 【Methods】32 rats were randomly divided into in blank control group, Ⅰ plateau frostbite group, Ⅱ plateau frostbite group and Ⅲ plateau frostbite group, 8 rats in each group. A lowpressure composite cabin was used to simulate plateau environment . After 2 weeks of hypoxia in rats, I to III degree plateau frostbite models were constructed by controlling the liquid nitrogen freezing time, and the control group was not treated. 48 h after modeling in each group, ultrasonic imaging was conducted by the intravenous Sovovue on the frozen part of the body for blood microcirculation imaging, and the frostbite parts of tissues of rats in each group were stained by HE staining.The pathological changes of different degree frostbitten tissue were observed. 【Results】 Blocked blood flow of the three groups was significantly increased with the increased degree of frostbite on plateau frozen tissue (P<0.05); Ⅰ plateau frozen tissue shew epidermis injury with leakage of red blood cell; Ⅱ plateau frostbite injury was suffered to the deep dermis and muscle surface; Ⅲ degree of plateau frostbite destroyed the almost all the muscle layer (P<0.05). 【Conclusion】 Ⅰ~Ⅲ plateau frostbite models can be directly constructed by controlling the liquid nitrogen freezing time .This approach has strong operability. 2015 Vol. 32 (10): 1873-1878 [Abstract] ( 82 ) HTML (1 KB)  PDF  (0 KB)  ( 45 ) 1876 Analysis of Target Monitoring and Risk Factors of Nosocomical Infection in ICU Hot! LI Yanping,QIAO Pu,LU Tingting,et al 【Objective】 To investigate the target monitoring and risk factors of nosocomial infection in ICU, and to put forward the preventive measures specifically to control nosocomial infection. 【Methods】 1,289 patients in ICU admitted to the hospital from 1st January 2013 to 31st December 2013 were chosen to calculate the average severity of illness (ASIS), the adjusted incidence of nosocomial infection, the invasive operation related infections and other relevant indicators. High risk factors for infection were analyzed . 【Results】 ICU patients with nosocomial infection covered 66 cases (5.12%), the daily incidence of infection rate was1.036% . After adjustment the infection rate was 1.98‰. The quarterly hospital infection showed a downward trend. The average severity of illness was 2.58, and the incidence of nosocomial infection was 1.98% after adjustment of the severity of the disease,;The most common infections were those related invasive operation : ventilator associated with pneumonia, vascular catheter related bloodstream infection , and catheter related urinary tract infection. The pathogens were mainly gram negative bacteria, with a total of 183 strains (90.59%),and gram positive bacteria took 19 (9.41%). 【Conclusion】 The infection rate of ICU was so high mainly because of 3 kinds of invasive operation related infections; Dynamic changes in ICU infection information can timely be accurated through the access of target monitoring so as to develop interventions to reduce the incidence of nosocomial infection in ICU. 2015 Vol. 32 (10): 1876-1878 [Abstract] ( 60 ) HTML (1 KB)  PDF  (0 KB)  ( 46 ) 1879 Effect Analysis of DangguiShaoyaosan on Cfos and Iba1 Expression in Spinal Trigeminal Subnucleus Caudalis after the Rat Experimental Tooth Movement Hot! LI Hongshi,LI Dongxia,FENG Yan,et al 【Objective】To investigate the effect of DangguiShaoyaosan on the expression of the carcinogen cfos and Iba1(the microglia membrane specific marker) in spinal trigeminal subnucleus caudalis after the  experimental tooth movement(ETM) on rats.【Methods】36 rats were randomly divided into the sham Group , the ETM Group and the DSS Group （DangguiShaoyaosan ）, Chewinglike jaw movements(VCM) at different time points and  Western blot protein expression changes of each SpVc fos and Iba1 immunohistochemistry 1 day after tooth movement were detected respectively.【Results】 The VCM of  ETM group reached the peak value 1 day after loading, then decreased gradually. Compared with the ETM group ,the DSS group began to decrease at 4 h, 12 h (P<0.05), the decrease appeared most significantly 1d after tooth movement (P<0.01) and the ETM group had no significant difference with the sham group at the same time point (P>0.05)；SpVc at the Sham group had very little cfos and Iba1 expression, which appeared negative or light staining, small cell body , weak projection  expression, and scattered in the distribution whose morphology is unclear.The expression of positive cells in the ETM group significantly increased, the staining deepened, the cell body became larger, the morphology was clear and full, and the distribution of the cells was deeper towards SpVc ; SpVc had only a small amount of cfos and Iba1 expression in the DSS group, and the cellbody of immune positive microglia was smaller and the cytoplasm was darker, while the projection appeared weakly positive.The expression of cfos in ETM group was significantly higher than that in group Sham and DSS, and the difference was statistically significant (P<0.05), while there was no significant difference between the Sham group and the DSS group (P>0.05).【Conclusion】Experimental tooth movement on rats is able to locally activate the expression of cfos and Iba1 in SpVc area , and DSS treatment can weaken activation of SpVc neurons and glial cells by tooth remove and reduce their spontaneous pain caused by tooth moving. 2015 Vol. 32 (10): 1879-1885 [Abstract] ( 56 ) HTML (1 KB)  PDF  (0 KB)  ( 34 ) 1882 Effect of Edaravone Combined with Mannitol on Expression of AQP4 mRNA After ischemic Brain Edema in Rats Hot! LI Dongmei,ZHANG Hongyan,ZHANG Lixin,et al 【Objective】The aim of this study was to investigate the change of aquaporin4（AQP4）after treatment edaravone and mannitol in ischemic brain edema rats.【Methods】150 adult male Wistar rats were randomly divided into randomized to six groups：normal, false surgery, surgery, mannitol（M ), edaravone（E）, and edaravone combined with mannitol（E with M）．Modified Longa rat brain infarction model (middle cerebral artery occlusion) were applied. The morphological changes of ischemic brain and AQP4 mRNA expression were detected after surgery of middle cerebral artery occlusion and measured treatment at 6h, 1d, 3d, 5d and 7d after each treatment。【Results】Within the ischemic brain, the intercellular space and perivascular space became larger, some neurons showed necrosis phenotype, and the edema was most obvious at 3d and was relieved at 7d. After the treatment with mannitol, the edema was not alleviated and few neurons were seen. In the groups of E and E with M, the cerebral edema was alleviated and more neurons were found seen. After the cerebral infarction, the expression of AQP4 mRNA increased, peaked at 3d, then decreased and returned to almost normal almostly at 7d（P<0.05）. AQP4 mRNA of group M was expressed less at the beginning first time, but then increased rapidly（P<0.05） .The expression of AQP4 in the group E and E with M remained low P<0.05）.【Conclusion】AQP4 plays an important role in the formation of ischemic cerebral edema. The expression of AQP4 mRNA increased after the cerebral infarction increased. Through the treatment of edaravone combined with mannitol, the expression of AQP4 mRNA decreased and the brain edema was alleviated. 2015 Vol. 32 (10): 1882-1885 [Abstract] ( 26 ) HTML (1 KB)  PDF  (0 KB)  ( 35 ) 1886 Study of LowDose CT with Fast Reconstruction upon the GPU Applying in the Temporal Bone Hot! LING Shoujia，MO Yan，HUANG Linbin,et al 【Objective】 To search the available scanning program of temporal bone to reduce radiation dose and meet the diagnostic requirements upon the Graphic processing unit (GPU) improvement of the speed of SAFIRE reconstructions. 【Methods】 80 cases of adult patients with temporal bone CT scan in our hospital were selected and randomly assigned to the control group (routine scanning, 120 kV tube voltage, tube current 120 MAS) and the observation group (80 kV tube voltage, tube current 120 MAS), each group of 40 cases. The control group images were reconstructed by conventional FBP, and the observation group was treated with conventional FBP, SAFIRE and SAFIRE reconstructed by GPU. The dose length product (DLP) of the two groups were recorded to calculate the effective radiation dose of ED, and the time of the reconstruction method was recorded, the noise (SD) and CT values of the images were recorded, and the signal to noise ratio (SNR) was calculated. All data were analyzed by statistical methods.【Results】The effective radiation dose in observation group was lower than that in control group, and the SD value in the observation group was significantly higher than that in control group, SNR significantly decreased, the difference was statistically significant (P<0.05), and the SD of the observation group was significantly lower than that of FBP reconstruction, SNR was significantly increased, and the difference was statistically significant (P<0.05); After SAFIRE reconstruction, the images of the observation group were significantly lower than that of SD reconstruction, and the SNR was significantly higher, and the difference was statistically significant (P< 0.05); The image quality of the observation group was poor, but the image quality was only slightly lower than the A group after SAFIRE reconstruction, it can meet the diagnostic requirements; the SD value was slightly higher, and the SNR value was slightly lower, but the difference does not have statistical significance (P>0.05).【Conclusion】 The scanning program (80KV, 120mAs) of temporal bone reconstructed by SAFIRE can meet the diagnostic requirements and reduce the radiation dose obviously, and GPU can reduce the time of reconstruction and improve the work efficiency. 2015 Vol. 32 (10): 1886-1889 [Abstract] ( 52 ) HTML (1 KB)  PDF  (0 KB)  ( 47 ) 1889 Effect Analysis of the Combined Application of Laparoscope and Uterine Artery Embolization in the Treatment of Uterine Fibroids Hot! LI Ruijuan，SUN Yalin，LIU Qinghua，et al 【Objective】To investigate the clinical efficacy of the combined application of laparoscope and uterine artery embolization in the treatment of uterine fibroids. 【Methods】120 patients with uterine fibroids were selected and divided into 3 groups based on different operation mode,40 cases for each. Group A were applicated with laparoscopic surgery, while group B  with uterine artery embolization, and group C combined application of laparoscope and uterine artery embolization. Surgery time, the amount of bleeding during surgery, the hospitalization time, recovery and efficacy after surgery were observed.【Results】Relapse rates were 22.5%, 7.5% and 2.5% for A,B, and C group respectively. Compared with the other two groups, relapse rates of the combined application of laparoscope and uterine artery embolization was significantly lower (χ2＝7.312, 7.433; P＝0.001, 0.001). Efficiency were 77.5%, 82.5% and 95% each group. Compared with the other two groups, the efficiency of group C was significantly higher (χ2＝6.831, 7.125；P＝0.004, 0.002).  【Conclusion】 The effect of the combined application of laparoscope and uterine artery embolization in the treatment of uterine fibroids was better than laparoscopic surgery or uterine artery embolization individually with lower recurrence rate. It is worthy of popularization and application. 2015 Vol. 32 (10): 1889-1891 [Abstract] ( 97 ) HTML (1 KB)  PDF  (0 KB)  ( 39 ) 1892 Experience of TransUmbilical SingleIncision Laparoscopic Cholecystectomy with Traditional Instruments：31 Case Report Hot! XU Dong,JIANG Honglei,YU Hao,et al 【Objective】The objective of this study is to discuss the security and feasibility of transumbilical singleincision laparoscopic cholecystectomy with the use of traditional instruments. 【Methods】Retrospective analysis of the Modus Operandi and postoperative recovery of 31 patients who received transumbilical singleincision laparoscopic cholecystectomy with traditional instruments was conducted.【Results】The operation process went smoothly in all of the 31 patients with the time of operation between 40 and 205 minutes(mean 88.9±123.6 min). There were no complications like bleeding, biliary fistula, or incisional hernia postoperation. The length of stay in hospital after the operation was between two to six days, with no obvious scar on the napes. 【Conclusion】Transumbilical singleincision laparoscopic cholecystectomy with traditional instruments is therefore deemed safe, feasible, effective, and beautiful. 2015 Vol. 32 (10): 1892-1893 [Abstract] ( 60 ) HTML (1 KB)  PDF  (0 KB)  ( 37 ) 1894 Expression of HE4 and CA125 in Ovarian Cancer Ascites Hot! CHEN Ying,ZHENG Lilin，YUAN XiaoQing 【Objective】To investigate the clinical significance of HE4 protein and cancer antigen125（CA125）in ovarian cancer ascites．【Methods】51 cases of epithelial ovarian cancer (the ovarian cancer group), 21 cases of the ovarian borderline tumor group and 15 cases of the control group (including 2 cases of tuberculous peritonitis, 2 cases of liver cirrhosis, 11 cases of acute and chronic pelvic inflammatory disease)were selected.ELISA and radioimmunity method were used to detect ascites, serum HE4 and CA125 protein expression.【Results】The positive rates of HE4 protein in ascites and serum of the ovarian borderline tumor group and the ovarian cancer group were significantly higher in than that in the control group（P<0.05）．In ovarian cancer of different histological types, the positive expression of ascites in the endometrium ,the serous carcinoma and HE4   was significantly increased. In the early stage (Ⅰ/Ⅱ)of ovarian cancer, the sensitivity of HE4  in serum was significantly higher than that of CA125 (P<0.05). In ovarian cancer, the specificity of ascites and serum HE4 was 100%, which was significantly higher than that of CA125 (P<0.05).【Conclusions】Increased protein expression of HE4 were closely associated with tumorigenesis and pathological behaviors of ovarian cancer, which  may be used as specific tumor markers to predict the earlier ovarian cancer．Combination of HE4 and CA125 measurement in ascites has an higher diagnostic value in differentiate ovarian benign and malignant tumors. 2015 Vol. 32 (10): 1894-1889 [Abstract] ( 78 ) HTML (1 KB)  PDF  (0 KB)  ( 39 ) 1897 Study of Rosuvastatin Combined with Irbesartan for the Improvement of Carotid Artery Flow Hot! ZENG Yan，CHEN Weiqin 【Objective】To investigate the efficacy of rosuvastatin combined with irbesartan on the improvement of carotid artery flow in patients with carotid artery atherosclerosis.【Methods】Sixty outpatients in our hospital from 2013 January to January,2014 who were confirmed of carotid atherosclerotic plaque by ultrasonography were randomly divided into two groups(Group A and Group B ), 30 patients in each group. Patients in Group A were given rosuvastatin (10mg/day, Oral)and irbesartan(150mg/day, Oral) for 12 months while Group B was given rosuvastatin (150mg/day, Oral ) only in the same period. The changes of serum lipid levels, carotid artery and carotid blood flow velocity were examined before and after treatment.【Results】Of 60 patients after treatment, the levels serum total cholesterol (TC), Triacylglycerol(TG), low density lipoprotein cholesterol (LDLC) were significantly lower than those before treatment (P<0.05). After the treatment, the carotid blood flow of Group A was improved obviously compared with those before treatment, and the difference was statistically significant (P<0.05) , which was better than that of Group B, the difference is statistically significant (P<0.05), and the carotid artery blood flow in Group B had no significant difference before and after the treatment (P>0.05).【Conclusion】Rosuvastatin combined or not combined with irbesartan can reduce TC, TG, LDLC in carotid atherosclerosis patients with hyperlipidemia, but the combination can effectively improve the carotid artery flow. 2015 Vol. 32 (10): 1897-1899 [Abstract] ( 76 ) HTML (1 KB)  PDF  (0 KB)  ( 43 ) 1900 Effects of VATS, Minimally Invasive Incision and Conventional Thoracotomy on Perioperative Pulmonary Function in Patients Undergoing Pulmonary Lobectomy Hot! JIANG Wenjun,LIU Siyang,TIAN Ye,et al 【Objective】To detect the effects of videoassisted thoracoscopic surgery (VATS), minimally invasive incision and conventional thoracotomy on perioperation pulmonary function in patients undergoing pulmonary lobectomy. 【Methods】Retrospective analysis of Our hospital's Cardiothoracic Surgery Department, October 2011 to July 2014, were treated 100 cases of lobectomy patients with clinical data, according to the surgical route divided into VATS group (30 cases), minimally invasive group (34 cases) , thoracotomy group (36 cases). compared Three groups of changes in lung function in patients before and after surgery were compared in retrospective analysis. 【Results】Preoperative, three groups of patients with differences in FVC, FEV1, MVV, PEF values of three groups of patients were measured value difference was not statistically significant (P>0.05); postoperative, 1,3,5,and 7d of FVC, FEV1, MVV, PEF measured values were measured and the differences were statistically significant (P<0.05). FVC, FEV1, MVV,and PEF measurements in the VATS group was significantly higher than that of minimally invasive thoracic surgery group and thoracotomy group (P<0.05); minimally invasive thoracic surgery group and the group the differences between values in the latter two groups were was not statistically significant (P>0.05). Preoperative and 1, 3d postoperative 1,3d the three groups of patients had no statistically significant differences in with PaO2 and PaCO2 measured value difference was not statistically significant (P>0.05),however,5 and 7d postoperative,PaO2 values of the VATS group were significantly higher than those of the other two groups. the VATS group PaO2 after the first minimally invasive group 5,7d and thoracotomy group, PaCO2 measured values were significantly lower than those of the other two groups minimally invasive thoracic surgery group and group (P<0.05). 【Conclusion】Implemented using The use of VATS lobectomy has a smaller effect on lung function and blood gas index of patients than the on the more minimally invasive and conventional thoracotomy lobectomy. small, The differences between minimally invasive and conventional thoracotomy lobectomy for lung function and blood gas index the impact is insignificant. 2015 Vol. 32 (10): 1900-1906 [Abstract] ( 93 ) HTML (1 KB)  PDF  (0 KB)  ( 36 ) 1903 Expression of Neutrophil Surface Ahesion Molecule Lymphocyte Function Associated Antigen 1 in Patients with the Preeclampsia Disease Hot! XIANG Lanhua,TAN Xiaoping 【Objective】The purpose of this study is to examine the expression of the neutrophil surface adhesion molecule lymphocyte function associated antigen 1（LFA1） and its relationship with preeclampsia disease (PE) patients. 【Methods】Between June 2012 and June 2014, 50 patients with PE were selected as subjects for a research project. These patients were divided into two groups in accordance with their specific medical condition: the severe PE group (20 cases), and the mild PE group (30 cases). During the duration of the experiment, 40 healthy pregnant women were selected as normal control group. The mother's blood and the blood in the umbilical cord were both found to contain neutrophils and neutrophil surface adhesion molecules FLA1 expression. Correlation analysis wasconducted. 【Results】The neutrophil count and neutrophil surfaceadhesion molecule LFA1 positive rate in the blood of the PE group mothers was found to be higher than the control group, with the difference being quite statistically significant (P<0.05). It was also found that as the PE illness aggravated, both the maternal bloodneutrophil count and neutrophil surfaceadhesion molecule LFA1 positive rate showed an increasing trend (P<0.05). In addition, maternal blood neutrophil count, neutrophil surface adhesion molecules LFA1 positive rate in the gestational diabetes group were higher than that of group without gestational diabetes, and the difference was statistically significant (P<0.05); In another analysis of the PE group , the umbilical cord blood neutrophil count and neutrophil surface adhesion molecules LFA1 positive rate were also higher than the control group, with the difference being statistically significant once again (P<0.05), and as the PE illness aggravated, both umbilical cord blood neutrophil count as well as neutrophil surface adhesion molecules LFA1 positive rate showed a trend of increased (P<0.05); Mother blood neutrophils surface expression of adhesion molecules LFA1 positive rate showed a trend of increased positively correlated with systolic blood pressure in patients with PE (r=0.597,P=0.597). Results showed that a mother's bloodneutrophil surface expression of adhesion molecules (LFA1) positive rate directly correlated with the systolic blood pressure in patients with PE (r=0.597,P=0.597), the diastolic blood pressure also showed a direct correlation (r= 0.526,P=0.526). 【Conclusion】Excessive neutrophil activation in patients with PEas well as excessive neutrophil activation is associated with PE severity. From our findings, we can now inhibit the excessive activation of neutrophils in PE pregnancy cases in complications such as prevention and control. 2015 Vol. 32 (10): 1903-1906 [Abstract] ( 67 ) HTML (1 KB)  PDF  (0 KB)  ( 38 ) 1907 Clinical Characteristics of Obese and Non Obese Women with Polycystic Ovary Syndrome Hot! XU Zhaoxia 【Objective】To analyze different type clinical features of patients with polycystic ovary syndrome. 【Methods】110 cases of patients with multiple ovarian syndrome were selected in our hospital from January 2012 to December 2012 , patients were divided into obese and nonobese group according to BMI (BMI≥25 kg/m2 ). Clinical index, sex hormone levels and complications of the two groups were compared . 【Results】The incidence of hirsutism, acne of the nonobese group was higher than that of the obese patients, and the incidence of amenorrhea, menstrual thinning and high androgen levels was lower, and the difference had statistical significance (P<0.05). There was no significant difference in the levels of estrogen (E2), prolactin (PRL) and serum follicle stimulating hormone (FSH) in the two groups (P>0.05). The patients with hyperinsulinemia , insulin resistance and diabetes were higher than those in non obese group, the difference was statistically significant (P<0.05), There was no significant difference in the incidence of impaired glucose tolerance between the two groups (P>0.05) .【Conclusion】The clinical characteristics of the patients of polycystic ovary syndrome either with obesity or non obesity have a big difference, thus a personalized treatment program should be developed on the patient's own situation . 2015 Vol. 32 (10): 1907-1912 [Abstract] ( 54 ) HTML (1 KB)  PDF  (0 KB)  ( 27 )

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