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Abstract 【Objective】To investigate the effect of DangguiShaoyaosan on the expression of the carcinogen cfos and Iba1(the microglia membrane specific marker) in spinal trigeminal subnucleus caudalis after the experimental tooth movement(ETM) on rats.【Methods】36 rats were randomly divided into the sham Group , the ETM Group and the DSS Group （DangguiShaoyaosan ）, Chewinglike jaw movements(VCM) at different time points and Western blot protein expression changes of each SpVc fos and Iba1 immunohistochemistry 1 day after tooth movement were detected respectively.【Results】 The VCM of ETM group reached the peak value 1 day after loading, then decreased gradually. Compared with the ETM group ,the DSS group began to decrease at 4 h, 12 h (P<0.05), the decrease appeared most significantly 1d after tooth movement (P<0.01) and the ETM group had no significant difference with the sham group at the same time point (P>0.05)；SpVc at the Sham group had very little cfos and Iba1 expression, which appeared negative or light staining, small cell body , weak projection expression, and scattered in the distribution whose morphology is unclear.The expression of positive cells in the ETM group significantly increased, the staining deepened, the cell body became larger, the morphology was clear and full, and the distribution of the cells was deeper towards SpVc ; SpVc had only a small amount of cfos and Iba1 expression in the DSS group, and the cellbody of immune positive microglia was smaller and the cytoplasm was darker, while the projection appeared weakly positive.The expression of cfos in ETM group was significantly higher than that in group Sham and DSS, and the difference was statistically significant (P<0.05), while there was no significant difference between the Sham group and the DSS group (P>0.05).【Conclusion】Experimental tooth movement on rats is able to locally activate the expression of cfos and Iba1 in SpVc area , and DSS treatment can weaken activation of SpVc neurons and glial cells by tooth remove and reduce their spontaneous pain caused by tooth moving.
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Received: 03 February 2015
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2015, Vol. 32 
