GADD45α对肝硬化大鼠联合肝脏分隔和门静脉结扎术后肝脏再生的调控作用

doi:10.3969/j.issn.1671-7171.2022.12.005

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摘要【目的】探讨GADD45α对肝硬化大鼠联合肝脏分隔和门静脉结扎术(ALPPS)后肝脏再生的调控作用。【方法】选取经高脂饲料喂养及四氯化碳诱导的90只雄性SD肝硬化大鼠,随机分为三组,即ALPPS组、手术对照组及正常对照组,每组30只。ALPPS组给予联合肝脏分隔和门静脉结扎的ALPPS,手术对照组仅行开关腹及肝周韧带游离,正常对照组自然喂养。各组分别于术后1 d、7 d、14 d处死10只大鼠,采用Real-time PCR分析大鼠GADD45α基因表达情况,Western blotting检测大鼠GADD45α蛋白表达情况,分析并比较三组大鼠不同时间点肝功能及肝脏再生率。【结果】术后14 d,ALPPS组大鼠血清ALT、AST水平均低于术后1 d、术后7 d,且低于正常对照组、手术对照组,差异均有统计学意义(P<0.05);ALPPS组大鼠GADD45α mRNA、GADD45α蛋白表达均高于术后1 d、术后7 d,且高于正常对照组、手术对照组,差异均有统计学意义(P<0.05);ALPPS组大鼠肝脏再生率高于术后1 d、术后7 d,且高于正常对照组、手术对照组,差异均有统计学意义(P<0.05)。【结论】GADD45α对肝硬化大鼠联合肝脏分隔和门静脉结扎术后肝脏再生具有调控作用,可提高大鼠肝脏再生率。

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关键词 ：肝硬化,实验性, 大鼠, 疾病模型,动物, 肝再生

Abstract：【Objective】To discuss GADD45α regulatory effect of liver regeneration in cirrhotic rats after combined associating liver partition and portal vein ligation for staged hepatectomy(ALPPS). 【Methods】Ninety male SD cirrhotic rats fed with high-fat diet and induced by carbon tetrachloride were randomly divided into three groups:ALPPS group, surgical control group and normal control group, with 30 rats in each group. The ALPPS group was treated with ALPPS combined with hepatic septation and portal vein ligation. The operation control group only opened and closed the abdominal and perihepatic ligaments, and the normal control group was fed naturally. Ten rats in each group were killed at 1 day, 7 days and 14 days after operation. The expression of rat GADD45α gene was analyzed by Real time PCR, and the expression of rat GADD45α protein was detected by Western blotting. The liver function and liver regeneration rate of the three groups at different time points were analyzed and compared.【Results】On the 14th day after operation, the levels of serum ALT and AST in the ALPPS group were lower than those on the 1st and 7th day after operation, and also lower than those in the normal control group and the operation control group (P<0.05); The expression of GADD45α mRNA and GADD45α protein in the ALPPS group was significantly higher than that in the normal control group and the operation control group (P<0.05); The liver regeneration rate of rats in the ALPPS group was higher than that in 1 day and 7 days after operation, and higher than that in the normal control group and the operation control group (P<0.05). 【Conclusion】GADD45α has regulatory effect on liver regeneration after combined liver septation and portal vein ligation in cirrhotic rats, and can improve the rate of liver regeneration in rats.

Key words： Liver Cirrhosis, Experimental Rats Disease Models, Animal Liver Regeneration

收稿日期: 2022-10-31

中图分类号:

R575.2

基金资助:* 湖南省自然科学基金面上项目(2018JJ2302)

引用本文:

谭新华, 刘鹏, 魏源水, 于奕, 朱朝庚. GADD45α对肝硬化大鼠联合肝脏分隔和门静脉结扎术后肝脏再生的调控作用[J]. 医学临床研究, 2022, 39(12): 1775-1777.
TAN Xin-hua, LIU Peng, WEI Yuan-shui, et al. Regulatory Effect of GADD45α of Liver Regeneration after Combined Hepatic Septation and Portal Vein Ligation in Cirrhotic Rats. JOURNAL OF CLINICAL RESEARCH, 2022, 39(12): 1775-1777.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2022.12.005 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2022/V39/I12/1775

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