Abstract：【Objective】To explore the relationship between high risk human papillomavirus (HR-HPV)-negative cervical lesions and cervical microenvironment. 【Methods】A total of 90 patients with HR-HPV negative cervical lesions were selected as the study subjects, including 26 patients with low-grade squamous intraepithelial lesion (LSIL group), 31 patients with high-grade squamous intraepithelial lesion (HSIL group), and 33 patients with cervical squamous cell carcinoma (CSCC group). The positive expression levels of CD4, CD8, Foxp3 and CD163 in the cervical lesions and the levels of IL-2 and IL-10 in the vaginal lavage fluid were detected and compared. 【Results】 Lactobacillus, Gardnerella and Bacillus were the most common vaginal flora in HPV negative cervical lesions; The detection rate of Lactobacillus in the CSCC group was lower than that in the LSIL group and the HSIL group, while the detection rate of Gardnerella and bacillus was higher than that in the LSIL group and the HSIL group. Compared with patients in LSIL and HSIL groups, the number of CD4+ T cells in cervical lesions of the CSCC group decreased, while the number of CD8+ T cells, Foxp3+ T cells and CD163+ tumor associated macrophages increased (P<0.05). Compared with the patients in the LSIL and HSIL groups, the level of IL-2 in vaginal lavage fluid in the CSCC group decreased, but the level of IL-10 increased (P<0.05). 【Conclusion】Vaginal micro ecology and immune imbalance are closely related to the progression of HR-HPV negative cervical lesions. Targeted treatment of vaginal infection and improvement of immunity can prevent the progression of cervical lesions.
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2022, Vol. 39 
