Effects of Metabolites of Endophytic Bacterium RPB-32 from Radix Pseudostellariae on Blood Biochemical Indexes in Mice
XIE Hong-ya, SU Song, WANG Jun-li, et al
Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education; School of Public Health,Guizhou Medical University,Guiyang Guizhou 550025
Abstract：【Objective】 To investigate the effect of fermentation metabolites of endophytic bacterium RPB-32 from Radix Pseudostellariae on blood biochemical indexes in mice. 【Methods】Petroleum ether,ethyl acetate and n-butanol were successively used to extract the broth of strain RPB-32;The organic solvent extracts of endophytic bacterium RPB-32 were prepared by decompressing.A certain concentration of organic solvent extracts were prepared with 3% ethanol (solvent control),and then gavaged into KM mice. The eyeballs were removed in batches on the 7th and 14th days,and the blood biochemical indexes were detected by automatic biochemical analyzer. 【Results】Of different organic extracts,there was no significant difference in total protein (TP),albumin (ALB),alanine aminotransferase (ALT),aspartate aminotransferase (AST) and urea nitrogen (BUN) between the three concentration treatment groups and the solvent control group (P>0.05);The activity of alkaline phosphatase (ALP) in the high-dose n-butanol extract group was lower than that in the solvent control group,and the difference was statistically significant (P<0.05).【Conclusion】The metabolites of endophytic bacterium RPB-32 of Radix Pseudostellariae heterophylla had no harmful effect on the body. This suggests that it may have the effects of preventing arteriosclerosis,hypertension and diabetes,protecting kidney and liver,inhibiting harmful bacteria such as bacillus coli,and promoting the growth of beneficial bacteria such as Lactobacillus,but its specific mechanism needs to be further studied.
谢虹雅, 苏松, 王俊丽, 欧阳湖, 焦松林, 任建国. 太子参内生菌RPB-32发酵代谢物对小鼠血液生化指标的影响[J]. 医学临床研究, 2022, 39(6): 804-807.
XIE Hong-ya, SU Song, WANG Jun-li, et al. Effects of Metabolites of Endophytic Bacterium RPB-32 from Radix Pseudostellariae on Blood Biochemical Indexes in Mice. JOURNAL OF CLINICAL RESEARCH, 2022, 39(6): 804-807.
2022, Vol. 39 
