Abstract：【Objective】To investigate the protective effect of endostatin on acute lung injury in septic mice and to explore its mechanisms behind these protective effects.【Methods】One hundred and fifty male (n=150) Kunming mice were randomly divided into the following groups: sham, septic model and endostatin treatment groups. The septic model was established by cecal ligation and puncture(CLP). Mice in the treatment group were administrated endostatin (2mg/kg, ip) after the operation while those in the sham and septic model group were treated with equivalent amount of saline instead. Survival rates were observed for up to 3 days. The effects of endostatin on the expression of TNF-α, IL-6 and VEGF in the serum were assessed. In addition, we examined the effects of endostatin on Evans blue leakage, wet to dry weight ratio, histology and the phosphorylation of p38 MAPK proteins in lung tissues of septic mice.【Results】We found that endostatin increased the survival of septic mice and reduce the Evans blue leakage of lung tissues, lung wet to dry weight ratio and morphologic changes. In addition, endostatin reduced serum TNF-α, IL-6 and VEGF-C levels in septic mice and inhibit phosphorylation of p38 in lung tissues of septic mice.【Conclusions】Endostatin can inhibit inflammation and improve vasopermeability and regulate the p38 MAPK signaling pathway in lung and exert protective effect on acute lung injury of septic mice.
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2017, Vol. 34 
