Abstract：【Objective】To investigate the effect of icariin(ICA) on the expression of miR-21-5p in osteolysis induced by titanium particles. 【Methods】Six-week-old male Balb/c mice were randomly divided into sham operation group(SOP group), the titanium particle group(TiP group) and the icariin group (ICA group).In the ICA Group, titanium particles were used to induce osteolysis in mice, and 300 mg/kg of icariin was given daily for 14 days by intragastric administration after the operation. Real-time PCR was used to detect the relative expression of miR-21-5p in mice serum before the operation and 3 days, 7 days, 14 days after administration of icariin. The relative expression of miR-21-5p in skull of mice was detected by Real-time PCR at 14 days after icariin administration. 【Results】There was no difference in the relative expression of serum miR-21-5p in each group before osteolysis-induced by titanium particles. Compared to the SOP Group, the relative expression of miR-21-5p in serum and skull from the TiP Group was significantly decreased after the operation (P<0.01). Compared to the TiP Group, the relative expression of miR-21-5p in serum and skull of the ICA group was significantly increased (P<0.01). 【Conclusion】Icariin can significantly promote the expression of miR-21-5p in serum and skull of mice after osteolysis induced by titanium particles. These results suggest that mir-21-5p can be used as a target for osteolysis therapy.
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2019, Vol. 36 
