Abstract：【Objective】 To study the regulation effect of stachydrine on tumor growth and the expression of CyclinD1, c-myc, Bcl-2, c-Met, p53, p16, Bax, Caspase-3 in gastric cancer xenograft tumors mice. 【Methods】After cultured SGC-7901 cell line of human gastric cancer, the cell suspension was inoculated into BALB / C nude mice subcutaneously to obtain the transplanted tumor model of gastric xenograft cancer. The mice were randomly divided into two groups: the control group treated with normal saline and the STA group treated with stachydrine . The volume and weight of the transplanted tumor and the expression of these oncogenes were measured after 21 days.【Results】The volume and weight of the transplanted tumor in the STA group were significantly lower than those in the control group; the protein expression of CyclinD1, c-myc, Bcl-2 and c-Met in the transplanted tumor was significantly lower than that in the control group, while the protein expression of p53, p16, Bax and Caspase-3 were significantly higher than that in the control group. 【Conclusion】Stachydrine can inhibit the growth of gastric cancer transplanted tumor mice, down regulate the expression of proto oncogene and up regulate the expression of tumor suppressor gene.
纪红梅, 李安庆, 王峰. 水苏碱对胃癌移植瘤小鼠肿瘤生长及基因表达的影响[J]. 医学临床研究, 2020, 37(3): 347-349.
JI Hong-mei, LI An-qing, WANG Feng. Effect of Stachydrine on Tumor Growth and Gene Expression in Gastric Xenograft Cancer in Mice. JOURNAL OF CLINICAL RESEARCH, 2020, 37(3): 347-349.
[1] Casamayor M, Morlock R, Maeda H,et al. Targeted literature review of the global burden of gastric cancer[J].Ecancer,2018, 12: 883. [2] 兰蓝, 赵飞, 蔡玥, 等. 中国居民2015年恶性肿瘤死亡率流行病学特征分析[J].中华流行病学杂志, 2018, 39(1): 32-34. [3] 赵林钢, 刘兆国, 杨爱华. 水苏碱通过调控 ACTG2 蛋白表达抑制结肠癌生长[J].中国药学杂志, 2018, 53(13): 1077-1082. [4] 胡文斌, 张婷, 秦威, 等. 江苏省昆山市1981～2015年去胃癌死因期望寿命与潜在减寿年分析[J].中国肿瘤, 2017, 26(10): 786-791. [5] Wang M, Shu ZJ, Wang Y, et al. Stachydrine hydrochloride inhibits proliferation and induces apoptosis of breast cancer cells via inhibition of Akt and ERK pathways[J].Am J Transl Res,2017, 9(4):1834-1844. [6] Liu Y, Wei S, Zou Q, et al. Stachydrine suppresses viability & migration of astrocytoma cells via CXCR4/ERK & CXCR4/Akt pathway activity[J].Future Oncol,2018, 14(15):1443-1459. [7] 王军, 赵醒, 赵宇阳, 等. CyclinD1、细胞分裂周期蛋白C25B与胃癌的相关性[J].中国老年学杂志, 2018, 38(6): 1456-1458. [8] Gurung SK, Dana S, Mandal K, et al. Downregulation of c-Myc and p21 expression and induction of S phase arrest by naphthalene diimide derivative in gastric adenocarcinoma cells[J].Chem Biol Interact,2019, 1(304): 106-123. [9] Soylemez F, Ay OI, Ay E, et al. Effect of EGF on Bax, Bcl-2 and Fas expression in ulcerous disease and N87 cell line[J].Bratisl Lek Listy,2017, 118(11):695-701. [10] Huang KH, Sung IC, Fang WL, et al. Correlation between HGF/c-Met and Notch1 signaling pathways in human gastric cancer cells[J].Oncol Rep,2018, 40(1):294-302. [11] Li W, Luo S, Ma G, et al. Impact of liver kinase B1 on p53 and survivin and its correlation with prognosis in gastric cancer[J].Onco Targets Ther,2019, 22(12): 1439-1445. [12] Chung T, DO SI, Na K, et al. Stromal p16 overexpression in gastric-type mucinous carcinoma of the uterine cervix[J].Anticancer Res,2018, 38(6):3551-3558. [13] 檀碧波, 李勇, 赵群, 等. 胃癌组织中miR-218和凋亡相关蛋白Bcl-2、Bax、xIAP、Survivinm RNA的表达观察[J].山东医药, 2018, 58(47): 20-23. [14] 刘小敏, 李巧云, 陈灿斌, 等. PIG11、Caspase-3蛋白在胃癌中的表达及临床意义[J].现代生物医学进展, 2018, 18(4): 657-661. [15] 杨静, 齐洁敏, 王明娟, 等. Caspase-3和c-IAP1在胃癌组织中的表达及意义[J].医学与哲学, 2018, 39(10): 40-43.
2020, Vol. 37 
