蛛网膜下腔出血大鼠鼻咽全氟化合物处理对其神经保护作用研究

doi:10.3969/j.issn.1671-7171.2021.04.029

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摘要 【目的】 探究蛛网膜下腔出血大鼠鼻咽全氟化合物处理(NP-PFC)对其神经保护作用及作用机制。【方法】 40只大鼠经造模后随机均分为模型组(B组)、NP-PFC处理组(C组),另随机选取20只大鼠作为假手术组(A组)。通过对各组大鼠进行神经功能评价、脑组织切片HE染色、MDA含量的测定、SOD及CAT活性检测、脑组织RT-PCR实验、脑组织Wenstern blot实验,对NP-PFC的神经保护作用进行探究。【结果】 A、B、C组大鼠的神经功能评分分别为(16.9±2.4)分、(11.6±2.2)分、(15.1±2.4)分,B组、C组大鼠神经功能评分低于A组,C组高于B组,差异具有统计学意义(F=26.64,P<0.001)。A组大鼠前额叶皮质中各层细胞结构清晰,神经细胞边缘清晰;B组大鼠前额叶皮质中各层细胞排列稀疏散乱,神经细胞边缘模糊;C组大鼠前额叶皮质中各层细胞排列情况较B组大鼠相比明显改善,其神经细胞边缘也较B组大鼠清晰。三组大鼠脑组织中MDA、SOD及CAT含量比较,差异具有统计学意义(F=331.94、473.27、275.82,P<0.05)。A、B、C三组大鼠脑组织中caspase-3 mRNA表达量分别为(0.63±0.11)、(1.42±0.26)、(0.77±0.19),差异具有统计学意义(F=46.04,P<0.001)。A、B、C三组大鼠脑组织中caspase-3蛋白表达量分别为(0.32±0.08)、(0.72±0.13)、(0.39±0.09),差异具有统计学意义(F=43.60,P<0.001)。【结论】 NP-PFC可通过抑制氧化应激及神经细胞的凋亡保护蛛网膜下腔出血大鼠的神经系统。

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	王传宝
	刘晶晶

关键词 ：蛛网膜下腔出血/病因学, 大鼠, Wistar, 氟化物/治疗应用, 神经保护

Abstract：【Objective】 To explore the neuroprotective effect and mechanism of nasopharynx perfluorochemical (NP-PFC) treatment in rats with subarachnoid hemorrhage (SAH). 【Methods】 After modeling, forty rats were randomly divided into two groups: model group (group B), NP-PFC treatment group (group C), and another 20 rats were randomly selected as sham operation group (group A). After administration, we evaluated neurological function and morphology of prefrontal cortex (HE staining). The number of apoptotic cells was measured by TUNEL staining. Then, we detected the content of MDA and the activity of SOD and CAT. Finally, the expression of caspase-3 mRNA and caspase-3 protein in brain tissue were detected by PCR and Western Blotting. 【Results】 The neurological function scores of rats in groups A, B, and C were (16.9±2.4), (11.6±2.2), and (15.1±2.4) points, respectively. The neurological function scores of rats in groups B and C were lower than those in group A, while the score in the group C was higher than that in the group B; and the difference was statistically significant (F=26.64, P<0.001). The cell structure of each layer in the prefrontal cortex of the group A rats was in normal pattern, and the edges of nerve cells are clear. The cells of each layer in the prefrontal cortex of group B rats were sparsely arranged, and the edges of nerve cells were fuzzy. When NP-PFC was used, the messy arrangement in the group C was significantly improved compared to the group B, and the edges of nerve cells were clearer than that of group B. Comparison of the contents of MDA, SOD and CAT in the brain tissues of the three groups of rats showed statistically significant differences (F=331.94, 473.27, 275.82, P<0.05). The expression of caspase-3 mRNA in the brain tissues of rats in groups A, B and C were (0.63±0.11), (1.42±0.26), (0.77±0.19), respectively, and the difference was statistically significant (F=46.04, P<0.001). The expression of caspase-3 protein in the brain tissues of rats in groups A, B and C were (0.32±0.08), (0.72±0.13), (0.39±0.09), respectively, and the difference was statistically significant (F=43.60, P< 0.001).【Conclusion】 NP-PFC has neuroprotective effect on the morphology of brain tissue in SAH rats. And it can decrease the quantity of apoptotic cells through oxidative stress inhibition.

Key words： Subarachnoid Hemorrhage/ET Rats, Wistar Fluorides/TU Neuroprotection

收稿日期: 2019-06-28

中图分类号:

R743.35

通讯作者: * E-mail:420854344@qq.com

引用本文:

王传宝, 刘晶晶. 蛛网膜下腔出血大鼠鼻咽全氟化合物处理对其神经保护作用研究[J]. 医学临床研究, 2021, 38(4): 579-582.
WANG Chuan-Bao, LIU Jing-jing. Neuroprotective Effect and Mechanism of NP-PFC Treatment in Rats with Subarachnoid Hemorrhage. JOURNAL OF CLINICAL RESEARCH, 2021, 38(4): 579-582.

链接本文:

http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2021.04.029 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2021/V38/I4/579

[1] 张光绪,马骏,王成斌,等.自发性蛛网膜下腔出血后早期脑损伤发生机制及实验性治疗研究进展[J].临床神经外科杂志,2016,13(6):474.
[2] CUI J B,CHEN Q Q,LIU T T, et al. Risk factors for early-onset ventilator-associated pneumonia in aneurysmal subarachnoid hemorrhage patients[J].Braz J Med Biol Res,2018,51(7):25.
[3] WOLFSON M R,MALONE D J,WU J, et al. Intranasal perfluorochemical spray for preferential brain cooling in sheep[J].Neurocrit Care,2008,8(3):437-447.
[4] WANG K C,TANG S C,LEE J E,et al. Impaired microcirculation after subarachnoid hemorrhage in an in vivo animal model[J].Sci Rep,2018,8(1):133.
[5] LI R,LIU W,YIN J,et al.TSG-6 attenuates inflammation-induced brain injury via modulation of microglial polarization in SAH rats through the SOCS3/STAT3 pathway[J].J Neuroinflammation,2018,15(1):231.
[6] HU Q, LI T, WANG L, et al. Neuroprotective effects of a smoothened receptor agonist against early brain injury after experimental subarachnoid hemorrhage in rats[J].Front Cell Neurosci,2016,10:306.
[7] 涂盛,邵安文,盛吉芳.富氢盐水对蛛网膜下腔出血大鼠神经保护作用的机制研究[J].浙江医学,2018,40(8):803.
[8] MA J. Pramipexole-induced hypothermia reduces early brain injury via PI3K/AKT/GSK3β pathway in subarachnoid hemorrhage rats[J].Sci Rep,2016,6:23817.
[9] AN H, DUAN Y,WU D, et al. Phenothiazines enhance mild hypothermia-induced neuroprotection via PI3K/Akt regulation in experimental stroke[J].Sci Rep,2017,7(1):7469.
[10] FU P,HU Q. 3,4-dihydroxyphenylethanol alleviates early brain injury by modulating oxidative stress and Akt and nuclear factor-κB pathways in a rat model of subarachnoid hemorrhage[J].Exp Ther Med,2016,11(5):1999-2004.
[11] DONG Y S,WANG J L,FENG D Y, et al. Protective effect of quercetin against oxidative stress and brain edema in an experimental rat model of subarachnoid hemorrhage[J].Int J Med Sci,2014,11(3):282-290.
[12] LI H,LV B,KONG L,et al.Nova1 mediates resistance of rat pheochromocytoma cells to hypoxia-induced apoptosis via the Bax/Bcl-2/caspase-3 pathway[J].Int J Mol Med,2017,40(4):1125-1133.