Abstract：【Objective】 To to study the effects of acute cerebral infarction on the levels of dendritic cells (DCS) and inflammatory factors in rats.【Methods】 Twenty 8-week-old female SD rats were randomly divided into experimental group and control group, with 10 rats in each group. The middle cerebral artery occlusion (MACO) rat model was made by suture method. The operation steps of the control group were the same as those of the experimental group, but the suture was not inserted. Immunofluorescence, immunohistochemistry and ELISA were performed 7 days after operation. Expression levels of DCs and interleukin-1β (IL-1 β), Interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) in the experimental group and the control group were observed.【Results】 Compared with the control group, the quantity of positive cells of CD74 and integrinαE in the ischemic brain tissue of the experimental group was bigger, and the difference was statistically significant (P<0.05);The expression of IL-1β,IL-6 and TNF-α in ischemic brain tissue of rats in experimental group was significantly higher than that in the control group (P<0.05);The difference of the contents of serum IL-1β,IL-6 and TNF- α of the two groups was statistically significant (P<0.05). 【Conclusion】Cerebral infarction induces the expression of DCs and inflammatory factors in rats, and the inflammatory response is a pathological process of acute cerebral infarction.
钟真真, 徐平, 王凯, 张小波. 急性脑梗死对大鼠树突状细胞和炎症因子水平的影响[J]. 医学临床研究, 2021, 38(10): 1515-1518.
ZHONG Zhen-zhen, XU Ping, WANG Kai, et al. Effects of Acute Cerebral Infarction on the Levels of Dendritic Cells and Inflammatory Factors in Rats. JOURNAL OF CLINICAL RESEARCH, 2021, 38(10): 1515-1518.
[1] NAGHAVI M,WANG H,LOZANO R, et al.Global, regional, and national age-sex specific all-cause and cause-specific mortality for 240 causes of death, 1990-2013[J].Lancet,2015,385(9963):117-171. [2] JEONG HG,KO SB,KIM CK,et al. Tachycardia burden in stroke unit is associated with functional outcome after ischemic stroke[J].Int J Stroke,2016,11(3):313-20. [3] SU J,LIU J,YAN X Y,et al. Cytoprotective effect of the UCP2-SIRT3 signaling pathway by decreasing mitochondrial oxidative stress on cerebral ischemia-reperfusion injury[J].Int J Mol Sci,2017,18(7):1599-1607. [4] LAING R J,JAKUBOWSKI J,LAING R W.Middle cerebral artery occlusion without craniectomy in rats.Which method works best?[J].Stroke,1993,24(2):294-297. [5] LONGA E Z,WEINSTEIN P R,CARLSON S,et al.Reversible middle artery occlusion without craniectomy in rats[J].Stroke,1989,20(1):84-91. [6] IADECOLA C,J ANRATHER.The immunology of stroke: from mechanisms to translation[J].Nat Med,2011,17(7):796-808. [7] SANTOS S C,PELOSI P,LEME S P.Immunomodulation after ischemic stroke: potential mechanisms and implications for therapy[J].Crit Care,2016,20(1):391. [8] KOSTULAS N,LI H L,XIAO B G,et al. Dendritic cells are present in ischemic brain after permanent middle cerebral artery occlusion in the rat[J].Stroke,2002,33(4):1129-1134. [9] TUTTOLOMONDO A,MAIDA C,PINTO A.Inflammation and inflammatory cell recruitment in acute cerebrovascular diseases[J].Curr Immunol Re,2015,11(1):24-32. [10] BOROVACANIN M,JOVANOVIC I,DEJANVIC SD,et al. Increase systemic levels of IL-23 as a possible constitutive marker in schizophrenia[J].Psychoneuroendocrinology,2015,56(1):143-147. [11] IBRAHIM R R,AMER R A,ABOZEID A A,et al. Micro RNA 146a gene variant / TNF-α/IL-6/IL-1 β; A cross-link axis inbetween oxidative stress,endothelial dysfunction and neuro-inflammation in acute ischemic stroke and chronic schizophrenic patients[J].Arch Biochem Biophys,2020,679(1):108-193.
2021, Vol. 38 
