Abstract：【Objective】 The aim of this study is to explore the expression and significance of Cdc42 (cell division cyclin42) in the hippocampus tissue of rats with neurotoxicity of sevoflurane. 【Methods】 Forty-two Sprague Dawley (SD) rats of 7 days old were randomly divided into three groups (n=14): group A, group B and group C. Rats in group A were exposed to 2% sevoflurane and 80% oxygen inhalation once for 2 hours. Rats in group B were exposed to 2% sevoflurane and 80% oxygen inhalation 2 hours/day for three consecutive days. Rats in group C received only 80% oxygen inhalation for 2 hours. Rats were sacrificed at 3 hours after experimental models completed and the hippocampus tissues were collected to measure the expression of Cdc42 and cleaved caspase3 (cysteine proteolytic enzyme 3, CI-Csp3) by western blot. Spatial learning and memory ability of adult rats were detected by Morris water maze (MWM) test at 45-49 days after birth. 【Results】 Compared to the group C, the expression of Cdc42 and Cl-Csp3 in the hippocampus of the group A had no significant difference (P>0.05), whereas the expression of Cdc42 and Cl-Csp3 in the hippocampus of group B increased significantly (P<0.05). In place navigation test, there was no significant difference of the escape latency between group C and group A (P>0.05). The escape latency of rats in the group B were significantly longer than that of C group (P<0.05). In probe trial test, the percent time in target quadrant in group A was not different from that of group C (P>0.05), While the percent time in target quadrant in group B decreased (P<0.05). 【Conclusion】 Multiple inhalation of sevoflurane can induce neurotoxicity and memory dysfunction, which may be related to the increase in the expression of Cdc42.
韩涛, 陈琴. Cdc42在七氟烷神经毒性反应大鼠海马组织中的表达及其意义[J]. 医学临床研究, 2020, 37(9): 1343-1346.
HAN Tao, CHEN Qin. Expression and Significance of Cdc42 in the Hippocampus Rat Tissue Caused by Sevoflurane Neurotoxicity. JOURNAL OF CLINICAL RESEARCH, 2020, 37(9): 1343-1346.
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2020, Vol. 37 
