高压氧对脑缺血再灌注大鼠神经功能的影响

doi:10.3969/j.issn.1671-7171.2021.09.022

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (1413 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要【目的】探讨高压氧对脑缺血再灌注大鼠神经功能的影响。【方法】选取60只Wistar大鼠,随机分成3组,假手术组、模型组和实验组,每组20只。假手术组大鼠仅进行手术过程而不造成缺血,模型组大鼠直接行大脑中动脉缺血再灌注,实验组大鼠采用高压氧预处理再行大脑中动脉缺血再灌注,对比三组大鼠再灌注24 h后神经功能缺损评分、脑梗死体积、缺血病灶脑组织神经细胞凋亡指数(AI),脑组织超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)活力、丙二醛(MDA)、一氧化氮(NO)含量变化。【结果】假手术组的脑组织没有受损,其神经功能缺损评分和脑梗死体积占比均为0。模型组和实验组脑组织均受到损伤,神经功能缺损评分和脑梗死体积及AI 这3种指标均升高,但实验组损伤较轻,其数据低于模型组,差异有统计学意义(P<0.05);三组大鼠再灌注24 h后脑组织SOD、LDH活力及MDA、NO含量比较,差异均有统计学意义(P<0.05)。模型组大鼠脑组织SOD、LDH活力均低于假手术组(P<0.05),但实验组大鼠脑组织SOD、LDH活力均高于模型组(P<0.05)。模型组大鼠脑组织MDA、NO均高于假手术组(P<0.05),但实验组大鼠脑组织MDA、NO活力均低于模型组(P<0.05)。【结论】高压氧可通过改善脑组织氧化应激损伤抑制大鼠脑缺血再灌注损伤。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章

关键词 ：脑缺血/治疗, 再灌注损伤, 高压氧, 疾病模型,动物, 神经系统, 大鼠

Abstract：【Objective】To investigate the effect of hyperbaric oxygen on neural function in rats with cerebral ischemia-reperfusion. 【Methods】A total of 60 Wistar rats was randomly divided into 3 groups: sham operation group, model group and experimental group, with 20 rats in each group. The rats in the sham operation group were only operated without ischemia. The rats in the model group were directly subjected to middle cerebral artery ischemia-reperfusion. The rats in the experimental group were subjected to high-pressure preconditioning and then subjected to middle cerebral artery ischemia-reperfusion. The neurological deficit score, cerebral infarct volume and neuronal apoptosis index (AI) of ischemic lesions in the three groups were compared 24 hours after reperfusion, The activities of superoxide dismutase (SOD), lactate dehydrogenase (LDH), malondialdehyde (MDA) and nitric oxide (NO) in brain tissue changed. 【Results】 The brain tissue in the sham operation group was not damaged, and the neurological deficit score and the proportion of cerebral infarction volume were 0. The brain tissue in the model group and the experimental group was damaged, and the neurological deficit score, cerebral infarction volume and AI were increased, but the injury in the experimental group was mild, and the data were lower than those in the model group (P<0.05); There were significant differences in the activities of SOD and LDH and the contents of MDA and NO in the brain tissue of the three groups 24 hours after reperfusion (P<0.05). The activities of SOD and LDH in the model group were lower than those in the sham operation group (P<0.05), but the activities of SOD and LDH in the experimental group were higher than those in the model group (P<0.05). The activities of MDA and NO in the model group were higher than those in the sham operation group (P<0.05), but the activities of MDA and NO in the experimental group were lower than those in the model group (P<0.05). 【Conclusion】 Hyperbaric oxygen can inhibit cerebral ischemia-reperfusion injury in rats by improving oxidative stress injury in brain tissue.

Key words： Brain Ischemia/TH Reperfusion Injury Hyperbaric Oxygenation Disease Models, Animal Nervous System Rats

收稿日期: 2021-06-16

中图分类号:

R743.31

基金资助:榆林市科协青年人才托举计划项目(20190115);榆林市科技计划项目(YF-2020-046)

通讯作者: **E-mail:46966592@qq.com

引用本文:

张伟靖, 赵斌, 刘霞, 董建彬, 乔晶晶. 高压氧对脑缺血再灌注大鼠神经功能的影响[J]. 医学临床研究, 2021, 38(9): 1356-1360.
ZHANG Wei-jing, ZHAO Bin, LIU Xia, et al. Effect of Hyperbaric Oxygen on Neurological Function in Rats with Cerebral Ischemia-Reperfusion. JOURNAL OF CLINICAL RESEARCH, 2021, 38(9): 1356-1360.

链接本文:

http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2021.09.022 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2021/V38/I9/1356

[1] KONG Q, MA X, WANG C, et al. Patients with acute ischemic cerebrovascular disease with coronary artery stenosis have more diffused cervicocephalic atherosclerosis[J].J Atheroscler Thromb,2019, 26(9):792-804.
[2] WU M Y, YIANG G T, LIAO W T, et al. Current mechanistic concepts in ischemia and reperfusion injury [J].Cell Physiol Biochem,2018, 46(4):1650-1667.
[3] CHENG J, ZHU P, QIN H, et al. Dexmedetomidine attenuates cerebral ischemia/reperfusion injury in neonatal rats by inhibiting TLR4 signaling [J].J Int Med Res,2018, 46(7):2925-2932.
[4] HUANG L, CHEN C, ZHANG X, et al. Neuroprotective effect of curcumin against cerebral ischemia-reperfusion via mediating autophagy and inflammation [J].J Mol Neurosci,2018, 64(1):129-139.
[5] SACLI H, KARA I, DILER M S, et al. The relationship between the use of cold and isothermic blood cardioplegia solution for myocardial protection during cardiopulmonary bypass and the ischemia-reperfusion injury [J].Ann Thorac Cardiovasc Surg,2019, 25(6):296-303.
[6] LI H Z, CHEN J F, LIU M, et al. Effect of hyperbaric oxyge patients with acute ischemic cerebrovascular disease with coronary artery stenosis have more diffused cervicocephalic atherosclerosis n on the permeability of the blood-brain barrier in rats with global cerebral ischemia/reperfusion injury [J].Biomed Pharmacother,2018, 108(7):1725-1730.
[7] 张小冲, 赵浩, 马建华, 等. 线栓法大鼠MCAO模型的实验研究[J].医学研究杂志, 2013, 42(6):49-55.
[8] 王龙, 金保哲, 张新中. 脑血流监测对大鼠脑缺血模型制备的评价作用[J].中国脑血管病杂志, 2017, 14(5):254-260.
[9] 张根葆. 病理生理学[M]. 北京市:高等教育出版社, 2014:89-90.
[10] LONGA E Z, WEINSTEIN P R, CARLSON S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats [J].Stroke,1989, 20(1):84-91.
[11] ZHANG Y P, ZHANG Y, XIAO Z B, et al. CFTR prevents neuronal apoptosis following cerebral ischemia reperfusion via regulating mitochondrial oxidative stress [J].J Mol Med (Berl),2018, 96(7):611-620.
[12] LU H, WANG B, CUI N, et al. Artesunate suppresses oxidative and inflammatory processes by activating Nrf2 and ROS-dependent p38 MAPK and protects against cerebral ischemia reperfusion injury [J].Mol Med Rep,2018, 17(5):6639-6646.
[13] ZORINA I I, FOKINA E A, ZAKHAROVA I O, et al. Features of the changes in lipid peroxidation and activity of Na⁺/K⁺-ATPase in the brain of the aged rats in the conditions of two-vessel cerebral ischemia/reperfusion [J].Adv Gerontol,2019, 32(6):941-947.
[14] HENTIA C, RIZZATO A, CAMPORESI E, et al. An overview of protective strategies against ischemia/reperfusion injury: The role of hyperbaric oxygen preconditioning [J].Brain Behav,2018, 8(5):51-59.
[15] RAJAN W D, WOJTAS B, GIELNIEWSKI B, et al. Dissecting functional phenotypes of microglia and macrophages in the rat brain after transient cerebral ischemia [J].Glia,2019, 67(2):232-245.
[16] 张爱民, 蒋宗滨. 高压氧预处理通过HIF-1α/VEGF通路减轻大脑缺血再灌注损伤[J].中国病理生理杂志, 2018, 34(11):136-141.
[17] ZHAO Y J, NAI Y, LI S Y, et al. Retigabine protects the blood-brain barrier by regulating tight junctions between cerebral vascular endothelial cells in cerebral ischemia-reperfusion rats [J].Eur Rev Med Pharmacol Sci,2018, 22(23):8509-8518.
[18] SHI Y S, ZHANG Y, LIU B, et al. Nomilin protects against cerebral ischemia-reperfusion induced neurological deficits and blood-brain barrier disruption via the Nrf2 pathway [J].Food Funct,2019, 10(9):5323-5332.
[19] 陈涵, 江春霞, 邵凌, 等. 高压氧预处理对大鼠小肠缺血再灌注损伤的保护作用[J].中华航海医学与高气压医学杂志, 2020, 27(1):64-68.
[20] 卞合涛, 段春霞, 黄卫. 高压氧对脑梗死后出血转化的治疗作用及其机制研究[J].中华神经医学杂志, 2019, 18(5):442-446.
[21] 余智, 于民, 邵晓莉, 等.Gly14-Humanin抑制脑缺血再灌注损伤炎症反应及细胞凋亡与NF-κB p65的相关性分析[J].中华全科医学, 2018, 16(2):214-217,256.
[22] LEE J W, LEE D H, PARK J K, et al. Sodium nitrite-derived nitric oxide protects rat testes against ischemia/reperfusion injury [J].Asian J Androl,2018, 21(1):92-97.
[23] 李静, 王继光, 张俊领. 高压氧预处理对CIRI大鼠神经功能的影响及机制[J].基因组学与应用生物学, 2018, 37(1):38-46.