Abstract：【Objective】To investigate the ultrastructural changes and pathogenesis of gastrointestinal tract in diabetic gastrointestinal disorders. 【Methods】A total of 50 SD rats were randomly divided into the control group (n=25) and the observation group (n=25). Animal model of diabetes combined with gastrointestinal dysfunction in the observation group were established with streptozotocin infection and diet-controlled method. Left ventricular end-diastolic diameter (LVEDD), end-systolic diameter (LVESD), systolic ventricular septal thickness (IVSs), left ventricular posterior wall thickness (LVPWs), and left ventricular ejection fraction(LVEF) were measured by echocardiography.Meanwhile, fasting blood glucose (FPG) levels were determined by blood glucose meter and fasting insulin (FINS) was measured by enzyme-linked immunosorbent assay (ELISA), while insulin resistance index (HOMA-IR) and insulin sensitivity index (ISI) were calculated by steady state model. Finally, rat gastrointestinal tissues of the two groups were taken and the ultrastructural changes were observed by transmission electron microscopy. 【Results】The levels of LVEDD, LVESD,FPG,FINS and HOMA-IR in the observation group were higher than those in the control group (P<0.05). The levels of IVSs, LVPWs, ISI and LVEF in the observation group were lower than those in the control group (P<0.05). The results of transmission electron microscopy showed that the ultrastructure of the gastric antrum and colon in the gastrointestinal tract tissue of laboratory diabetic rats was seen dramatic changes. While the ultrastructure of the small intestine changed relatively strongly. The lesions were mainly focused on smooth muscles and microvessels. No ultrastructural changes occurred in the nerve endings. At the same time, the arrangement of muscle cells was disordered, with larger cytosolic vacuoles within the muscle cells. Overall, no significant abnormalities were observed in the control rats. 【Conclusion】Diabetic rats are often accompanied by blood glucose level disorder and gastrointestinal dysfunction, which may be related to the ultrastructural changes of the gastrointestinal tract. Our research may provide some ideas for the treatment of diabetes.
刘冬燕, 杨静. 糖尿病大鼠胃肠功能紊乱时胃肠道的超微结构变化及其发病机制研究[J]. 医学临床研究, 2020, 37(6): 860-862.
LIU Dong-yan, YANG Jing. Ultrastructural Changes of Gastrointestinal Tract in Rat with Diabetic Gastrointestinal Disorders. JOURNAL OF CLINICAL RESEARCH, 2020, 37(6): 860-862.
2020, Vol. 37 
